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| 1 | 昆虫造血作用和造血干细胞研究进展显示文摘昆虫血细胞(insect hemocyte)在昆虫代谢、发育变态以及先天免疫等方面承担着重要的作用。昆虫只有先天免疫系统,血细胞所行使的免疫功能对于昆虫对抗外源病菌尤为重要。本文主要介绍了昆虫血细胞类型、造血作用、造血干细胞及造血相关因子的相关研究。通过特殊染色和形态学观察,果蝇Drosophila血细胞主要由3类细胞组成,而鳞翅目等大部分昆虫血细胞由5类细胞组成。昆虫血细胞主要存在于循环血液环境及造血器官内,而在这两个系统中都存在有进行复制的血细胞,这为研究昆虫造血干细胞特性和其定位提供了一个很好的系统。果蝇血细胞祖细胞来自于胚胎中胚层细胞,然后再分化为各种血细胞,这一系列分化过程由造血因子所调控。 | 谈娟 周其明 崔红娟 | 2011 | 昆虫学报2011，54，10： | 6 |
| 2 | Integrin β2 and β3:Two plasmatocyte markers deepen our understanding of the development of plasmatocytes in the silkworm Bombyx mori显示文摘Insect hemocytes play important biological roles at developmental stages,metamorphosis,and innate immunity.As one of the most abundant cell types,plasmatocytes can participate in various innate immune responses,especially in encapsulation and node formation.Here,2 molecular markers of plasmatocytes,consisting of integrinβ2 andβ3,were identified and used to understand the development of plasmatocytes.Plasmatocytes are widely distributed in the hematopoietic system,including circulating hemolymph and hematopoietic organs(HPOs).HPOs constantly release plasmatocytes with high proliferative activity in vitro;removal of HPOs leads to a dramatic reduction in the circulating plasmatocytes,and the remaining plasmatocytes gradually lose their ability to proliferate in vivo.Our results demonstrated that the release of plasmatocytes from HPOs is regulated by insulin-mediated signals and their downstream pathways,including PI3K/Akt and MAPK/Erk signals.The insulin/PI3K/Akt signaling pathway can significantly irritate the hematopoiesis,and its inhibitor LY294002 could inhibit the hemocytes discharged from HPOs.While the insulin/MAPK/Erk signaling pathway plays a negative regulatory role,inhibiting its activity with U0126 can markedly promote the discharge of plasmatocytes from HPOs.Our results indicate that the circulating plasmatocytes are mainly generated and discharged by HPOs.This process is co-regulated by the PI3K/Akt and MAPK/Erk signals in an antagonistic manner to adjust the dynamic balance of the hemocytes.These findings can enhance our understanding of insect hematopoiesis. | Kui Zhang Jingjing Su Xin Hu Xiaomin Yan Siyuan Chen Chongyang Li Guangzhao Pan Hongbo Chang Wenli Tian Muhammad Nadeem Abbas Hongjuan Cui | 2022 | Insect Science2022，29，6： | 0 |
| 3 | 利用不同染料复合染色判定家蚕血细胞的存活状况显示文摘家蚕有5种血细胞,可以通过吖啶橙(acridine orange,AO)和碘化丙啶(propidium iodide,PI)染色来区分。溴化噻唑蓝四氮唑[3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide,MTT]是一种广泛应用于细胞增殖和活细胞鉴定的染色剂。通过对家蚕血细胞进行复染,即先用MTT染色,再用AO和PI染色,发现以下2种情况:很多颗粒细胞、浆细胞和所有的原血球细胞不能被MTT染色,但AO染色显示为活的细胞;被MTT染色的细胞表面有紫色纤维状物伸出,除了一部分具有伪足的颗粒细胞外,所有被MTT染色的血细胞也被PI染色,细胞核是红色。因此,利用这3种染料对家蚕血细胞复合染色来判断细胞的存活与死亡,需要仔细分析并分别对待。 | 李旭全 杨兵 路岸瑞 刘朝良 凌尔军 | 2011 | 蚕业科学2011，37，4： | 0 |
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