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| 1 | 1株阿魏酸酯酶产生菌的筛选鉴定及发酵条件优化显示文摘从浙江绍兴鉴湖附近土样中分离筛选到1株产阿魏酸酯酶(ferulic acid esterase,FAE)的菌株FD-8,通过菌落形态、分生孢子梗形态对比和ITS/18S rDNA分子生物学同源性分析,鉴定该菌株为溜曲霉,命名为Aspergillus tamarii FD-8。FD-8生长最适碳源和氮源分别为蔗糖和酵母浸提物,最适生长温度和pH分别为32℃和5.0,最适产酶条件为32℃、pH 6.0,发酵周期为120 h。在最适培养基中、分阶段控制pH培养条件下,FAE比酶活达到231.34 mU/mg。去淀粉麸皮可诱导FAE的合成,在发酵48 h添加20.0 g/L去淀粉麸皮,FAE最高比酶活可达到573.61 mU/mg,比对照提高1.48倍。 | 曹艳 夏其乐 陈剑兵 陆胜民 | 2019 | 微生物学杂志2019，39，5： | 6 |
| 2 | Identification of Quantitative Trait Loci Affecting Hemicellulose Characteristics Based on Cell Wall Composition in a Wild and Cultivated Rice Species显示文摘房间墙 hemicellulosic 多糖是复杂、多样的在结构上。房间墙 hemicelluloses 和他们的生物角色的合成的知识是有限的。印射的量的特点 loci (QTL ) 是为为基因鉴定的复杂显型的解剖的一个有用工具。在这研究,我们在在普通野米饭, Yuanj,和与他们的基因渗入印射的栽培变种, Teqing,和执行 QTL 衬里的精英 indica (IL ) 之间的房间墙 monosaccharide 层次利用了自然变化。在 Yuanj 和 Teqing 的灰煤杆上进行的化学分析证明主要改变在葡萄糖和木糖层次被发现,它与特定的 hemicellulosic 聚合物被相关。Glycosidic 连接检查表明在 Yuanj,葡萄糖内容的增加源于混合连接 -glucan (MLG ) 的高水平,而在木糖内容的减小反映木聚糖脊梁和改变的 arabinoxylan (斧子) 结构的底层。为 monosaccharides 的十七 QTL 通过 95 核心 IL 的灰煤杆残余的作文分析被识别了。影响木糖和葡萄糖层次的四主要 QTL 分别地为 19 phenotypic 变化和 21% 负责。这研究为米饭房间墙形成的基因解剖提供一个唯一的资源并且在植物的机关改变。 | Si-Ju Zhang Xue-Qin Song Bai-Sheng YU Bao-Cai Zhang Chuan-Qing Sun J.Paul Knox Yi-Hua Zhou | 2012 | Molecular Plant2012，5，1： | 6 |
| 3 | Unraveling the Biochemical and Molecular Networks Involved in Maize Cell Habituation to the Cellulose Biosynthesis Inhibitor Dichlobenil显示文摘面对纤维素生合成禁止者 dichlobenil (DCB ) 涉及玉米细胞的使习惯到生长的生物化学、分子的过程被调查。DCB 在活跃、静止的生长阶段两个都影响纤维素的合成并且改变几 CesA 基因的表示。这些, ZmCesA5 和 ZmCesA7 似乎在使熟习起一个主要作用房间到生长面对 DCB。作为在纤维素的减小的后果,涉及 hydroxycinnamates 的合成的几基因的表示被增加,导致有 ferulic 和 p-coumaric 酸的高水平的房间墙。proteomic 分析表明到 DCB 的使习惯在几条新陈代谢的小径被连接到修正。最后,使熟习房间在除去的谷胱甘肽 S-transferase 介绍减小活动和抗氧化剂活动。到纤维素生合成要求的如此的关键进程的骚乱的植物房间改编在几个新陈代谢的网络变化,以便修改房间墙建筑学和新陈代谢,并且面对禁止者幸存。一些这些修正在这份报纸被描述。 | Hugo Melida Antonio Encina Jesus Alvarez Jose Luis Acebes David Caparros-Ruiz | 2010 | Molecular Plant2010，3，5： | 5 |
| 4 | Changes in Cinnamic Acid Derivatives Associated with the Habituation of Maize Cells to Dichlobenil显示文摘到象 dichlobenil (DCB ) 那样的纤维素生合成禁止者的房间文化的使习惯表示改进我们机制的知识的一个珍贵工具在植物房间墙中包含了结构的粘性。玉米房间线使熟习到 DCB 的致命的集中能通过在哪个纤维素被 arabinoxylans 的一个更广泛的网络部分代替的一面修改房间墙的获得成长。这个工作的目的是调查非使熟习、使熟习 DCB 的玉米房间文化的酉分的新陈代谢。玉米房间文化被喂[14C ] cinnamate 和在在整个文化周期的不同 intra 原形质、墙局部性的部分的放射性的命运被 autoradiography 并且焕发数分析。非使熟习、使熟习的文化显著地没处于他们举起的能力不同外长[14C ] 苯乙烯的酸。然而,有趣的差别在 radiolabeling 被发现低 -- 先生和高度 -- 先生代谢物。使熟习的文化显示了 radiolabeled 的一个更高的数字和数量低 -- 先生加重,它能充当以后为多糖 feruloylation 使用的储备。使熟习 DCB 的文化高度在 esterified 被充实[14C ] dehydrodiferulates 和更大的联合产品。在结论, hydroxycinnamates 的广泛、早的 cross-linking 在使熟习 DCB 的文化被观察,可能加强他们的纤维素缺乏的墙。 | Hugo Melida Jesus Alvarez Jose Luis Acebes Antonio Encina Stephen C. Fry | 2011 | Molecular Plant2011，4，5： | 4 |
| 5 | Identification of QTLs and candidate genes for physiological traits associated with drought tolerance in cotton显示文摘Background:Cotton is mainly grown for its natural fiber and edible oil.The fiber obtained from cotton is the indispensable raw material for the textile industries.The ever changing climatic condition,threatens cotton production due to a lack of sufficient water for its cultivation.Effects of drought stress are estimated to affect more than 50%of the cotton growing regions.To elucidate the drought tolerance phenomenon in cotton,a backcross population was developed from G.tomentosum,a drought tolerant donor parent and G.hirsutum which is highly susceptible to drought stress.Results:A genetic map of 10888 SNP markers was developed from 200 BC_2F_2 populations.The map spanned 4191.3 centi-Morgan(c M),with an average distance of 0.1047 c M,covering 51%and 49%of At and Dt sub genomes,respectively.Thirty stable Quantitative trait loci(QTLs)were detected,in which more than a half were detected in the At subgenome.Eighty-nine candidate genes were mined within the QTL regions for three traits:cell membrane stability(CMS),saturated leaf weight(SLW)and chlorophyll content.The genes had varied physiochemical properties.A majority of the genes were interrupted by introns,and only 15 genes were intronless,accounting for 17%of the mined genes.The genes were found to be involved molecular function(MF),cellular component(CC)and biological process(BP),which are the main gene ontological(GO)functions.A number of mi RNAs were detected,such as mi R164,which is associated with NAC and MYB genes,with a profound role in enhancing drought tolerance in plants.Through RT-q PCR analysis,5 genes were found to be the key genes involved in enhancing drought tolerance in cotton.Wild cotton harbors a number of favorable alleles,which can be exploited to aid in improving the narrow genetic base of the elite cotton cultivars.The detection of 30 stable QTLs and 89 candidate genes found to be contributed by the donor parent,G.tomentosum,showed the significant genes harbored by the wild progenitors which can be exploited in developing more robust cotton genotypes with diverse tolerance levels to various environmental stresses.Conclusion:This was the first study involving genome wide association mapping for drought tolerance traits in semi wild cotton genotypes.It offers an opportunity for future exploration of these genes in developing highly tolerant cotton cultivars to boost cotton production. | MAGWANGA Richard Odongo LU Pu KIRUNGU Joy Nyangasi CAI Xiaoyan ZHOU Zhongli AGONG Stephen Gaya WANG Kunbo LIU Fang | 2020 | Journal of Cotton Research2020，3，1： | 2 |
| 6 | 高效木聚糖降解酶系定制的新进展与挑战显示文摘木聚糖是植物细胞壁中含量最丰富的非纤维素多糖,大约占陆地生物质资源的20%-35%。不同物种来源的木聚糖结构因取代方式不同而具有广泛的异质性,这对生物质资源向生物燃料和其他高值产品高效转化提出了重大挑战。因此,需要开发由不同类型酶组成的最佳混合物以有效糖化木聚糖类底物。但是针对特定类型的底物设计高效降解酶系十分困难,应考虑底物的类型、底物的组成和物理性质、多糖的聚合度以及不同降解酶组分的生化性质等。本文从不同植物木聚糖的结构异质性与合成复杂性方面展示了其抗降解屏障,同时介绍了木聚糖主链降解酶系及侧链降解酶系的多样性以及协同降解作用,综述了复杂生境中微生物种群产生的混合酶系、降解菌株产生的高效酶系,以及基于特定木聚糖底物改造并定制简化高效的酶系统。随着不同种类木聚糖精细结构和木聚糖降解酶底物特异性的深入研究,针对特定底物类型进行绿色高效木聚糖酶系定制,加速木聚糖类底物的降解,从而实现木质纤维素资源的绿色高值化利用。 | 吴秀芸 戴琳 张舒 尚伟昊 余俊红 黄小平 王禄山 | 2020 | 微生物学通报2020，47，7： | 2 |
| 7 | Facile fabrication of the porous adsorbent from natural plant Angelica Sinensis stabilized liquid foam for dye removal显示文摘Porous materials as emerging high-efficiency adsorbents have attracted increasing attention in recent years,due to the high specific surface area,fast adsorption rate,favorable adsorption capacity to heavy metal,dyes,and other pollutants,etc.Among various approaches,the foam template method with the advantage of flexible operation and controllable pores structure is proposed to prepare net type of porous adsorbents with superior adsorption capacity and rapid adsorption rate.In this process,natural plant Angelica Sinensis(AS)was treated with a simple alkalineheat process,and the resultant product was used firstly to prepare foam with high stability,and the positive effect of the cellulose dissociation and the conversion of ligustilide into amphiphilic moleculars on improving the stability of Pickering foam was revealed.The liquid foam can serve as pore-foaming template and precursor of polymerization for synthesizing porous adsorbent by one-step integrated process of free radical polymerization and silane hydrolysis.The porous adsorbent showed the sufficient porous structure and excellent adsorption performance for cationic dye,with maximum adsorption capacities of 386.13 mg g^(-1) and 284.20 mg g^(-1) for methyl violet(MV)and malachite green(MG).In a word,the novel approach can provide an important reference for green synthesis of high stable foam and the superporous material,and the adsorbent showed great application potential in the field of water treatment. | Fangzhi Duan Yongfeng Zhu Hui Yu Aiqin Wang | 2022 | Green Chemical Engineering2022，3，1： | 1 |
| 8 | 高产阿魏酸酯酶菌株的筛选鉴定及产酶条件优化显示文摘为提高白酒酿造过程中阿魏酸的含量,该研究从浓香型白酒大曲中分离筛选产阿魏酸酯酶的菌株,通过形态观察、生理生化试验及分子生物学技术对其进行菌种鉴定,并以阿魏酸酯酶活力为评价指标,采用单因素试验及响应面试验对其发酵条件进行优化。结果表明,筛选得到一株产阿魏酸酯酶的菌株,编号为M2。经鉴定,其为一株蜡样芽孢杆菌(Bacillus cereus),该菌株产阿魏酸酯酶的最佳发酵条件为接种量5%,发酵温度30℃,初始pH值5.5,发酵时间72 h。在此优化条件下,阿魏酸酯酶活力为33.89 U/L,比优化前提高10.03%,具有较强的产阿魏酸酯酶能力。 | 王清龙 陈园园 刘延波 刘洪亮 王永华 朱金晓 张晓静 潘春梅 | 2022 | 中国酿造2022，41，10： | 1 |
| 9 | 阿魏酸酯酶及其产生菌株的功能特性及应用研究显示文摘阿魏酸酯酶(FAE)在生物加工、食品、医药、造纸、饲料等领域有很高的应用价值,目前已发现的产阿魏酸酯酶菌株的生产能力远远不能满足工业生产应用的要求,因此,开发适用于工业化生产和应用的阿魏酸酯酶尤为重要。从阿魏酸酯酶及其产生菌株的来源、分类、理化特性、形态特征、作用机制、功能及在畜禽生产中应用等方面进行了系统的阐述,为探索产阿魏酸酯酶菌株资源和研发廉价、高活力的阿魏酸酯酶及其相关研究提供参考。 | 杨晶晶 曹阳 | 2021 | 黑龙江八一农垦大学学报2021，33，6： | 1 |
| 10 | 二穗短柄草(Brachypodium distachyon)BdAD1基因的克隆、表达及功能分析显示文摘本研究利用生物信息学结合RT-PCR技术从二穗短柄草(Brachypodium distachyon)中克隆出BdAD1的c DNA基因,该基因编码一个包含500个氨基酸残基的乙醛脱氢酶家族蛋白。系统进化关系分析表明,该BdAD1蛋白序列与小麦(Triticum aestivum)、羊草(Leymus chinensis)和大麦(Hordeum vulgare)的同源蛋白具有较近的亲缘关系。BdAD1基因在植物细胞的细胞核和细胞质中均有表达,而且BdAD1蛋白兼具松柏醛脱氢酶和芥子醛脱氢酶的活性(CALDH/SALDH),可将松柏醛与芥子醛分别酶解生成阿魏酸和芥子酸,但它对松柏醛的催化效率显著高于芥子醛,因此推测BdAD1可能在苯丙烷代谢途径中对阿魏酸的合成具有重要的调控作用。 | 齐旭莉 肖亮 刘奕彤 沈红祥 刘清波 蒋建雄 | 2017 | 激光生物学报2017，26，2： | 0 |
| 11 | 水稻铝害研究进展显示文摘水稻(Oryza sativa L.)是世界上最重要的粮食作物之一,其种植的土壤pH常为酸性,易产生铝胁迫,导致产量下降且影响稻米的食用安全.水稻受到铝害时,会显著抑制根系生长,影响其生长发育.本文综述了水稻铝害发生的条件、缓解铝害的主要途径及作用机制、耐铝基因的鉴定和转录调控机制,为耐铝水稻品种的选育提供理论基础. | 李晟昱 周琪 何汶珈 乔景璇 李晓琳 包格根 | 2023 | 仲恺农业工程学院学报2023，36，1： | 0 |
| 12 | Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures显示文摘Maize(Zea mays L.) suspension-cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil(DCB), a cellulose biosynthesis inhibitor. Cellulose de ficiency was accompanied by marked changes in cell wall matrix polysaccharides and phenolics as revealed by Fourier transform infrared(FTIR) spectroscopy.Cell wall compositional analysis indicated that the cellulosede ficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol. In accordance with this, cellulose-de ficient cell walls showed a fivefold increase in Klason-type lignin.Thioacidolysis/GC-MS analysis of cellulose-de ficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to shortterm DCB-treatments. Gene expression analysis of these cells indicated an overexpression of genes specific for the biosynthesis of monolignol units of lignin. A study of stress signaling pathways revealed an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions. In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment. | Hugo Mélida Asier Largo-Gosens Esther Novo-Uzal Rogelio Santiago Federico Pomar Pedro García Penélope García-Angulo José Luis Acebes Jesús álvarez Antonio Encina | 2015 | Journal of Integrative Plant Biology2015，57，4： | 0 |
| 13 | 细胞壁结构对粗饲料纤维降解的因素分析显示文摘过去一直采用木质素的含量以及酸性洗涤纤维的含量来对饲草NDF降解率进行评估和预测,然而大量的研究表明,ADF和ADL的含量并不能说明NDF消化率的所有变化,说明细胞壁其它组分也影响NDF消化率。文章综合国内外相关研究来阐述木质素浓度、酚酸结构、木质素单体构成对NDF降解率的影响。 | 曹升旭 吴晓辉 郑飞 刘大森 | 2019 | 饲料工业2019，40，17： | 0 |
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