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| 1 | Altered Lignin Biosynthesis Improves Cellulosic Bioethanol Production in Transgenic Maize Plants Down-Regulated for Cinnamyl Alcohol Dehydrogenase显示文摘Cinnamyl 白酒脱氢酶(CAD ) 是涉及 monolignol 生合成的最后步的关键酶。木质素生产上的 CAD 下面规定的效果在玉米通过一条转基因的途径被调查。转基因的 CAD-RNAi 植物显示出在房间墙作文取决于分析织物和表演改变的酶的减小的不同的度。没有影响全部的木质素内容, CAD-RNAi 茎的细胞墙在 S-to-G 比率与细微减小包含木质素聚合物。另外,这些房间墙积累纤维素和 arabinoxylans 的高水平。相反, CAD-RNAi 中肋的房间墙在全部的木质素内容并且房间墙多糖介绍减小。,在 vitro degradability,试金显示出那到不同程度, CAD 活动的压抑导致的变化生产了中肋和比野类型的植物更可能减解的茎。在这块地里种的 CAD-RNAi 植物介绍了野类型的显型并且生产了干燥生物资源的更高的数量。有纤维质的 bioethanol 试金表明 CAD-RNAi 生物资源生产了乙醇的高水平与相比野类型,使 CAD 成为一个好目标两个都改进玉米 lignocellulosic 生物资源的营养、精力充沛的值。 | Silvia Fornale Montserrat Capellades Antonio Encina Kan Wang Sami Irara Catherine Lapierre Katia Ruel Jean-Paul Joseleaue Jordi Berenguer Pere Puigdomenech Joan Rigau David Caparros-Ruiz | 2012 | Molecular Plant2012，5，4： | 14 |
| 2 | Changes in Cinnamic Acid Derivatives Associated with the Habituation of Maize Cells to Dichlobenil显示文摘到象 dichlobenil (DCB ) 那样的纤维素生合成禁止者的房间文化的使习惯表示改进我们机制的知识的一个珍贵工具在植物房间墙中包含了结构的粘性。玉米房间线使熟习到 DCB 的致命的集中能通过在哪个纤维素被 arabinoxylans 的一个更广泛的网络部分代替的一面修改房间墙的获得成长。这个工作的目的是调查非使熟习、使熟习 DCB 的玉米房间文化的酉分的新陈代谢。玉米房间文化被喂[14C ] cinnamate 和在在整个文化周期的不同 intra 原形质、墙局部性的部分的放射性的命运被 autoradiography 并且焕发数分析。非使熟习、使熟习的文化显著地没处于他们举起的能力不同外长[14C ] 苯乙烯的酸。然而,有趣的差别在 radiolabeling 被发现低 -- 先生和高度 -- 先生代谢物。使熟习的文化显示了 radiolabeled 的一个更高的数字和数量低 -- 先生加重,它能充当以后为多糖 feruloylation 使用的储备。使熟习 DCB 的文化高度在 esterified 被充实[14C ] dehydrodiferulates 和更大的联合产品。在结论, hydroxycinnamates 的广泛、早的 cross-linking 在使熟习 DCB 的文化被观察,可能加强他们的纤维素缺乏的墙。 | Hugo Melida Jesus Alvarez Jose Luis Acebes Antonio Encina Stephen C. Fry | 2011 | Molecular Plant2011，4，5： | 4 |
| 3 | The biosynthesis and wall-binding of hemicelluloses in cellulose-deficient maize cells:An example of metabolic plasticity显示文摘Cell-suspension cultures(Zea mays L.,Black Mexican sweet corn) habituated to 2,6-dichlorobenzonitrile(DCB) survive with reduced cellulose owing to hemicellulose network modification. We aimed to de fine the hemicellulose metabolism modifications in DCB-habituated maize cells showing a mild reduction in cellulose at different stages in the culture cycle. Using pulse-chase radiolabeling, we fed habituated and non-habituated cultures with [3H]arabinose,and traced the distribution of 3H-pentose residues between xylans, xyloglucans and other polymers in several cellular compartments for 5 h. Habituated cells were slower taking up exogenous [3H]arabinose. Tritium was incorporated into polysaccharide-bound arabinose and xylose residues, but habituated cells diverted a higher proportion of their new [3H]xylose residues into(hetero) xylans at the expense of xyloglucan synthesis. During logarithmic growth, habituated cells showed slower vesicular traf ficking of polymers,especially xylans. Moreover, habituated cells showed a decrease in the strong wall-binding of all pentose-containing polysaccharides studied; correspondingly, especially in log phase cultures, habituation increased the proportion of 3H-hemicelluloses([3H]xylans and [3H]xyloglucan) sloughed into the medium. These findings could be related to the cel walls' cellulose-deficiency, and consequent reduction in binding sites for hemicelluloses; the data could also re fl ect the habituated cells' reduced capacity to integrate arabinox ylans by extra-protoplasmic phenolic cross-linking, as well as xyloglucans, during wall assembly. | María de Castro Janice G.Miller José Luis Acebes Antonio Encina Penélope García-Angulo Stephen C.Fry | 2015 | Journal of Integrative Plant Biology2015，57，4： | 2 |
| 4 | 纤维素生物合成抑制剂(CBI)类除草剂研究进展显示文摘在开发具有新作用机制的除草剂品种过程中,纤维素生物合成是潜在的理想靶标。此类除草剂既具有植物专一性又能有效避免田间抗性产生。概述了纤维素的生物合成以及CBI类除草剂的研究进展,包括种类、主要品种和相应的作用机制。 | 张秀兰 李正名 | 2013 | 世界农药2013，35，2： | 0 |
| 5 | Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures显示文摘Maize(Zea mays L.) suspension-cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil(DCB), a cellulose biosynthesis inhibitor. Cellulose de ficiency was accompanied by marked changes in cell wall matrix polysaccharides and phenolics as revealed by Fourier transform infrared(FTIR) spectroscopy.Cell wall compositional analysis indicated that the cellulosede ficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol. In accordance with this, cellulose-de ficient cell walls showed a fivefold increase in Klason-type lignin.Thioacidolysis/GC-MS analysis of cellulose-de ficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to shortterm DCB-treatments. Gene expression analysis of these cells indicated an overexpression of genes specific for the biosynthesis of monolignol units of lignin. A study of stress signaling pathways revealed an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions. In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment. | Hugo Mélida Asier Largo-Gosens Esther Novo-Uzal Rogelio Santiago Federico Pomar Pedro García Penélope García-Angulo José Luis Acebes Jesús álvarez Antonio Encina | 2015 | Journal of Integrative Plant Biology2015，57，4： | 0 |
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