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| 1 | 纳米二氧化钛的发育毒性研究显示文摘为了了解纳米TiO2对动物生长发育的影响,取怀孕昆明小鼠在妊娠6~17天内口服纳米TiO2,临产前剖腹产。检测其体重增加、脏器变化、胚胎重量、胚胎体长、胚胎尾长、死胎、畸胎、吸收胎等指标。统计分析显示,该纳米材料对怀孕雌鼠影响不大,但对胚胎却有一定的毒性作用。首次阐述了纳米TiO2具有一定的发育毒性。 | 薛猛 朱融融 孙晓宇 汪世龙 | 2009 | 材料导报2009，23，4： | 8 |
| 2 | 纳米TiO_2对PCR体系中DNA合成的影响及其机理研究显示文摘研究纳米TiO2对PCR体系中DNA合成的影响及其作用的分子机制.方法是把不同浓度的纳米TiO2直接加入PCR体系中,观察其对DNA合成量的影响;把与纳米TiO2预先混合的聚合酶、引物或模板的上层清液及沉淀分别加入到PCR体系中,观察预先相互作用对DNA合成量的影响;并用聚丙烯酰胺凝胶电泳和紫外可见光谱方法分析其作用的分子机制.结果表明锐钛矿型纳米TiO2对PCR体系中的DNA合成有浓度依赖性的抑制作用,且抑制作用强于微米级;聚合酶的上层清液、引物的沉淀、模板的上层清液与沉淀均有DNA的合成,但合成量均小于纳米TiO2直接加入PCR时的DNA的合成量;综合紫外可见光谱和电泳实验,认为避光条件下,锐钛矿型纳米TiO2抑制DNA合成的主要原因是对聚合酶、引物和模板的吸附作用,其中包括化学吸附和物理吸附,作用强度以引物最强,模板居中,聚合酶最弱,为进一步研究纳米TiO2对DNA的损伤及其生物毒性提供了理论基础. | 李世强 朱虹 朱融融 孙晓宇 姚思德 汪世龙 | 2008 | 中国科学(B辑)2008，38，3： | 3 |
| 3 | Impact and mechanism of TiO_2 nanoparticles on DNA synthesis in vitro显示文摘The impact of TiO2 nanoparticles on DNA synthesis in vitro in the dark and the molecular mechanism of such impact were studied. The impact of TiO2 nanoparticles on DNA synthesis was investigated by adding TiO2 nanoparticles in different sizes and at various concentrations into the polymerase chain reaction (PCR) system. TiO2 nanoparticles were premixed with the DNA polymerase, the primer or the template, respectively and then the supernatant and the precipitation of each mixture were added into the PCR system separately to observe the impact on DNA synthesis. Sequentially the interaction be- tween TiO2 nanoparticles and the DNA polymerase, the primer or the template was further analyzed by using UV-visible spectroscopy and polyacrylamide gel electrophoresis (PAGE). The results suggest that TiO2 nanoparticles inhibit DNA synthesis in the PCR system in the dark more severely than mi- croscale TiO2 particles at the equivalent concentration and the inhibition effect of TiO2 nanoparticles is concentration dependent. The molecular mechanism of such inhibition is that in the dark, TiO2 nanoparticles interact with the DNA polymerase through physical adsorption while TiO2 nanoparticles do with the primer or the template in a chemical adsorption manner. The disfunction levels of the bio-molecules under the impact of TiO2 nanoparticles are in the following order: the primer > the tem- plate > the DNA polymerase. | LI ShiQiang, ZHU Hong, ZHU RongRong, SUN XiaoYu, YAO SiDe & WANG ShiLong School of Life Science and Technology, Tongji University, Shanghai 200092, China | 2008 | Science China Chemistry2008，51，4： | 3 |
| 4 | Antitumor activity and pharmacokinetics of podophyllotoxin incorporated into solid lipid nanoparticles显示文摘To evaluate the antitumor activity and pharmacokinetics of podophyllotoxin(PPT) incorporated into solid lipid nanoparticles(SLN),Kunming mice inoculated with flesh tumor were used as animal model.The mice received a single daily intraperitoneal injection of PPT in 20% ethanol(5 mg/kg) and PPT-SLN(5 mg/kg in PPT) for 3 weeks.Gross tumor volumes,body weight and clinical observations were recorded daily.The mice were sacrificed for 24 h after the last administration,and the tumor inhibition rate was calculated with the tumor weight.For the pharmacokinetics research,the mice were treated with intraperitoneal injection of PPT(10 mg/kg) and PPT-SLN(10 mg/kg in PPT).Blood samples were collected at different time to determine the PPT concentration in plasma by HPLC.Blood drug level-time curve was made and pharmacokinetic parameters were calculated.As a result of drug administration,the tumor volume and weight of the mice injected with PPT-SLN were significantly restrained compared with mice treated with PPT or negative control.The tumor inhibition rate of 58.13% showed a significant antitumor activity of PPT-SLN.At the same time,the increased weight gain of the mice injected with PPT-SLN suggested a reduced toxicity of PPT in SLN.Pharmacokinetics study displayed a higher blood concentration,a prolonged circulation time,and an increased bioavailability of PPT-SLN compared with those of PPT.Our results demonstrated that PPT-SLN could optimize pharmacokinetics,enhance antitumor activity and attenuate toxicity,so it has a promising prospect for the application in anti-tumor treatment. | XUE Meng ZHU RongRong QIN LiLi LI FaJie LIU ZhiXue SUN XiaoYu WANG ShiLong | 2009 | Science China Chemistry2009，52，8： | 0 |
| 5 | DNA损伤检验点ATM-chk2通路影响衰老的作用机制分析显示文摘目的分析DNA损伤检验点ATM-chk2通路对影响衰老的作用机制。方法选择SD大鼠4个月龄、12个月龄、20个月龄和28个月龄各10只,分别取4个月龄、12个月龄、20个月龄和28个月龄大鼠的骨髓组织进行ATM-chk2通路的观察,并对结果进行分析。结果 4个月龄、12个月龄、20个月龄和28个月龄SD大鼠的ATM基因表达随着月龄增加,呈逐渐下降趋势,不同月龄大鼠间比较,差异具有显著性(P<0.05);ATM-chk2通路基因蛋白表达呈逐渐上升趋势,组间比较差异具有显著性(P<0.05)。结论 ATM基因表达及ATM-chk2通路基因蛋白表达水平和大鼠月龄直接相关,年龄越大ATM基因表达日渐降低,ATM-chk2通路基因蛋白表达呈逐渐上升趋势。 | 陈小敏 张素琴 | 2013 | 中国现代医生2013，51，15： | 0 |
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