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| 1 | 壳寡糖对脂多糖诱导猪空肠上皮细胞氧化损伤的作用显示文摘本试验旨在通过脂多糖(LPS)诱导猪空肠上皮细胞(IPEC-J2)来建立氧化应激模型,探讨壳寡糖(COS)的抗氧化作用效果。采用噻唑蓝(MTT)法检测LPS和COS对IPEC-J2作用12、24、48 h后细胞增殖活性的变化;选择适宜浓度LPS(1.0μg/m L)和COS(200μg/m L)作用于IPEC-J2,分为对照组、LPS组、COS组、LPS+COS组。采用Western Blot法检测核因子E2相关因子2(Nrf2)、血红素氧合酶-1(HO-1)蛋白的表达;试剂盒检测超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性及丙二醛(MDA)的含量。结果表明:1.0μg/m L的LPS对IPEC-J2作用24 h,细胞增殖的抑制率为41.6%,为造模的最适浓度和作用时间;COS在一定浓度范围内对IPEC-J2有增殖作用,其浓度为200μg/m L时效果最佳,作用时间为24 h时,细胞增殖率达到126.3%。LPS+COS组较对照组的SOD、CAT活性和M DA含量差异不显著(P>0.05),Nrf2和HO-1蛋白相对表达量显著升高(P<0.05);LPS+COS组较LPS组的Nrf2蛋白相对表达量显著升高(P<0.05),HO-1有升高趋势,但差异不显著(P>0.05),SOD、CAT活性显著升高(P<0.05),M DA含量显著降低(P<0.05)。由此可见,LPS诱导IPEC-J2氧化应激,而COS可进一步促进Nrf2和HO-1蛋白的高表达,并提高抗氧化酶的活性,降低氧化产物含量,从而增强细胞对氧化应激的抵抗力,起到保护作用。 | 肖定福 钟佳 刘进辉 李文平 | 2016 | 动物营养学报2016，28，7： | 9 |
| 2 | Natural heme oxygenase-1 inducers in hepatobiliary function显示文摘Many physiological effects of natural antioxidants,their extracts or their major active components,have been reported in recent decades.Most of these compounds are characterized by a phenolic structure,similar to that of α-tocopherol,and present antioxidant proper-ties that have been demonstrated both in vitro and in vivo.Polyphenols may increase the capacity of en-dogenous antioxidant defences and modulate the cellu-lar redox state.Changes in the cellular redox state may have wide-ranging consequences for cellular growth and differentiation.The majority of in vitro and in vivo studies conducted so far have attributed the protective effect of bioactive polyphenols to their chemical reac-tivity toward free radicals and their capacity to prevent the oxidation of important intracellular components.However,in recent years a possible novel aspect in the mode of action of these compounds has been sug-gested; that is,the ultimate stimulation of the heme oxygenase-1 (HO-1) pathway is likely to account for the established and powerful antioxidant/anti-inflam-matory properties of these polyphenols.The products of the HO-catalyzed reaction,particularly carbon mon-oxide (CO) and biliverdin/bilirubin have been shown to exert protective effects in several organs against oxidative and other noxious stimuli.In this context,it is interesting to note that induction of HO-1 expression by means of natural compounds contributes to protec-tion against liver damage in various experimental mod-els.The focus of this review is on the significance of targeted induction of HO-1 as a potential therapeutic strategy to protect the liver against various stressors in several pathological conditions. | Giovanni Li Volti David Sacerdoti Claudia Di Giacomo Maria Luisa Barcellona Antonio Scacco Paolo Murabito Antonio Biondi Francesco Basile Diego Gazzolo Raul Abella Alessandro Frigiola Fabio Galvano | 2008 | World Journal of Gastroenterology2008，14，40： | 2 |
| 3 | 关键的信号网络节点作为五没食子酰基葡萄糖的药效靶位的研究进展显示文摘五没食子酰基葡萄糖(PGG)是一种来自于多种药用植物的天然多酚化合物。使用体外细胞或体内动物模型的研究表明,PGG具有抗癌、抗糖尿病以及细胞保护的药物效应。对PGG生物学活性分子机制的研究逐渐发现其在细胞中具有各种药效靶位,而这些靶位是细胞信号转导网络中的关键节点,PGG的多靶位性与其药效多样性是相对应的。本文综述了PGG作用于细胞信号转导网络中各个节点从而实现其药学效应的研究进展。 | 石大禹 金小宝 朱家勇 | 2011 | 中国药理学与毒理学杂志2011，25，4： | 1 |
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