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| 1 | CO-EXPRESSION OF MACROPHAGE COLONY-STIMULATING FACTOR WITH ITS RECEPTOR IN HUMAN HEPATOMA CELLS AND ITS POTENTIAL ROLES显示文摘Objective: To investigate the potential role of macrophage colony-stimulating factor (M-CSF) and macrophage colony-stimulating factor receptor (M-CSFR) on the growth of human hepatoma cells. Methods:Specimens of different origin, including tissues of human hepatocellular carcinoma (HCC), human fetal liver (FL) and normal liver (NL), the hepatoma cell lines,as well as the peripheral blood mononuclear cells (PBMC) from patients with HCC or liver metastatic tumor (LMT), were used to detect the expression levels of M-CSF and M-CSF-R by ABC immunohistochemistry staining and reverse transcription polymerase chain reaction methods the expression levels of M-CSF and MCSF’R. Influence of monoclonal antibody against MCSF (BS) or M-CSF’R (RE2) on proliferation ability of hepatoma cell tilles in vitro was also studied. Results: The results showed that hepatoma tissues produced elevated levels of both M-CSF and M-CSF-R compared with those of fetal liver (P<0.001). The M-CSFIM’CSF-R expression levels of PBMC from hepatoma patients were higher than those of LMT patients (P<0.01, P<0.05) and the normal people (P<0.001). The hepatoma cell lines showed strong positive for M-CSF and M-CSF-R production. Both BS and REZ displayed a dosedependent inhibitory effect on the growth and proliferation of hepatoma cells. Conclusion: The study indicates a co-expression model for M-CSF-R in hepatoma cells, suggesting an involvement of M-CSFIMCSF-R in growth signaling of those malignant cells. The M-CSFIM-CSF-R seems to function through an autonomy mechanism in human hepatoma. | 杨文清 吴克复 宋玉华 赵明河 张陆松 宋乃国 张丽娜 | 1999 | Chinese Journal of Cancer Research1999，11，2： | 4 |
| 2 | 叶酸缺乏致胎鼠宫内发育迟缓及肝脏胰岛素生长因子系统表达变化显示文摘目的:叶酸是一种水溶性B族维生素,在体内氨基酸与核苷酸代谢中起重要作用,是胎儿生长发育所必须的营养素。本文通过建立叶酸缺乏的孕鼠模型,探讨叶酸缺乏对胎鼠宫内发育的影响,并研究胎鼠肝脏组织中胰岛素生长因子(IGF)系统的表达变化。方法:雌性C57BL/6J小鼠叶酸缺乏组6只、正常对照组6只,分别饲以不含叶酸和含2 mg叶酸/kg的纯合饲料。四周后与雄鼠交配,于怀孕第13.5天(13.5 dpc)对孕鼠剖腹取胎,观察和评价胎鼠发育指标,并对宫内发育迟缓(IUGR)比率进行统计。用Real-time PCR法检测胎鼠肝脏组织中胰岛素生长因子Ⅰ(IGFⅠ)、胰岛素生长因子Ⅰ受体(IGFⅠR)、胰岛素生长因子Ⅱ(IGFⅡ)、胰岛素生长因子Ⅱ受体(IGFⅡR)、胰岛素生长因子结合蛋白1(IGFBP-1)和胰岛素生长因子结合蛋白3(IGFBP-3)mRNA的相对表达水平。结果:叶酸缺乏组雌鼠合笼前每日体重增长量降低,13.5 dpc胎鼠吸收胎和死胎比率升高,胎重下降,IUGR比率显著升高,差异有统计学意义(P<0.05);叶酸缺乏组胎鼠肝脏组织中IGFⅡ和IGFⅡR mRNA的相对表达水平均低于正常对照组(P<0.05),IGFⅠ、IGFⅠR、IGFBP-1和IGFBP-3 mRNA的相对表达水平两组间没有差异(P>0.05)。结论:叶酸缺乏会导致小鼠孕中期胎鼠IUGR比率升高及胎肝IGFⅡ和IGFⅡR mRNA的表达水平降低,提示叶酸缺乏对IGF系统基因的调控,可能与胎鼠IUGR发生机制有关。 | 李屾 梁良 姚志刚 王理 李睿 梁春联 刘驰 | 2014 | 现代生物医学进展2014，14，4： | 2 |
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