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CO-EXPRESSION OF MACROPHAGE COLONY-STIMULATING FACTOR WITH ITS RECEPTOR IN HUMAN HEPATOMA CELLS AND ITS POTENTIAL ROLES

查看全文 作　　者：杨文清;吴克复;宋玉华;赵明河;张陆松;宋乃国;张丽娜 高影响力作者 出　　处：《Chinese Journal of Cancer Research》索引1999年第11卷第2期,共6页高影响力期刊 摘　　要：Objective: To investigate the potential role of macrophage colony-stimulating factor (M-CSF) and macrophage colony-stimulating factor receptor (M-CSFR) on the growth of human hepatoma cells. Methods:Specimens of different origin, including tissues of human hepatocellular carcinoma (HCC), human fetal liver (FL) and normal liver (NL), the hepatoma cell lines,as well as the peripheral blood mononuclear cells (PBMC) from patients with HCC or liver metastatic tumor (LMT), were used to detect the expression levels of M-CSF and M-CSF-R by ABC immunohistochemistry staining and reverse transcription polymerase chain reaction methods the expression levels of M-CSF and MCSF’R. Influence of monoclonal antibody against MCSF (BS) or M-CSF’R (RE2) on proliferation ability of hepatoma cell tilles in vitro was also studied. Results: The results showed that hepatoma tissues produced elevated levels of both M-CSF and M-CSF-R compared with those of fetal liver (P<0.001). The M-CSFIM’CSF-R expression levels of PBMC from hepatoma patients were higher than those of LMT patients (P<0.01, P<0.05) and the normal people (P<0.001). The hepatoma cell lines showed strong positive for M-CSF and M-CSF-R production. Both BS and REZ displayed a dosedependent inhibitory effect on the growth and proliferation of hepatoma cells. Conclusion: The study indicates a co-expression model for M-CSF-R in hepatoma cells, suggesting an involvement of M-CSFIMCSF-R in growth signaling of those malignant cells. The M-CSFIM-CSF-R seems to function through an autonomy mechanism in human hepatoma. 关 键 词：MACROPHAGE colony-stimulating FACTOR (MCSF) MACROPHAGE colony-stimulating FACTOR receptor(M-CSF-R) Hepatoma Co-expression, AUTOCRINE
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