|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 幽门螺杆菌细胞毒导致胃癌发生的作用机制显示文摘幽门螺杆菌(Helicobacter pylori,Hp)感染导致胃癌的重要原因之一是它的细胞毒作用。细胞毒素相关基因致病岛(cytotoxin-associated gene pathogenicity island,cagPAI)和空泡毒素A(vacuolatingcytotoxin A,vacA)是Hp最典型的细胞毒代表。cagPAI可诱发促炎因子的释放及增强促上皮细胞增殖信号的兴奋程度;vacA则导致上皮空泡化和病原体黏附。明确cagPAI和vacA在胃癌发生发展过程中的作用,将有利于全面理解Hp感染对机体造成的危害以及根治Hp感染的重要意义。 | 胡洁 孙哲 梅林 | 2014 | 中国药理学通报2014，30，2： | 16 |
| 2 | Helicobacter pylori infection- recent developments in diagnosis显示文摘Considering the recommended indications for Helicobacter pylori(H.pylori)eradication therapy and the broad spectrum of available diagnostic methods,a reliable diagnosis is mandatory both before and after eradication therapy.Only highly accurate tests should be used in clinical practice,and the sensitivity and specificity of an adequate test should exceed 90%.The choice of tests should take into account clinical circumstances,the likelihood ratio of positive and negative tests,the cost-effectiveness of the testing strategy and the availability of the tests.This review concerns some of the most recent developments in diagnostic methods of H.pylori infection,namely the contribution of novel endoscopic evaluation methodologies for the diagnosis of H.pylori infection,such as magnifying endoscopy techniques and chromoendoscopy.In addition,the diagnostic contribution of histology and the urea breath test was explored recently in specific clinical settings and patient groups.Recent studies recommend enhancing the number of biopsy fragments for the rapid urease test.Bacterial culture from the gastric biopsy is the gold standard technique,and is recommended for antibiotic susceptibility test.Serology is used for initial screening and the stool antigen test is particularly used when the urea breath test is not available,while molecular methods have gained attention mostly for detecting antibiotic resistance. | Ana Isabel Lopes Filipa F Vale Mónica Oleastro | 2014 | World Journal of Gastroenterology2014，20，28： | 12 |
| 3 | 幽门螺杆菌多态性与胃癌发生的风险的相关性研究显示文摘幽门螺杆菌(Helicobacter pylori,H.pylori)与胃癌的发生密切相关。不同菌株甚至同一菌株的不同株系之间存在遗传多样性,在基因的表型特征上有明显的分化,这些分化与基因组遗传信息的差异有关。基因组缩减可使细菌消耗更低的能量,需要较少的营养成分,这使东亚地区的H.pylori在增殖和生长方面优于其他地区。本文总结了H.pylori基因组的基本特征,分析了H.pylori基因组遗传多样性,探讨了基因组遗传多样性与胃癌的关系,有利于深入理解H.pylori的致病机理,为临床预防和治疗提供理论依据。 | 孔玲玲 董全江 田字彬 | 2016 | 胃肠病学和肝病学杂志2016，25，11： | 4 |
| 4 | CagA EPIYA polymorphisms in Colombian Helicobacter pylori strains and their influence on disease-associated cellular responses显示文摘AIM: To investigate the influence of the CagA diversity in Helicobacter pylori (H. pylori ) strains from Colombia on the host cell biology. METHODS: Eighty-four H. pylori-cagA positive strains with different Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs patterns, isolated from patients with gastritis (n=17), atrophic gastritis (n=17), duodenal ulcer (n=16), intestinal metaplasia (n=16) and gastric cancer (n=18), were included. To determine the integrity of the cag pathogenicity island (cag PAI) we evaluated the presence of cagA, cagT, cagE, and cag10 genes by polymerase chain reaction. AGS gastric epithelial cellswere infected with each strain and assayed for translo-cation and tyrosine phosphorylation of CagA by western blot, secretion of interleukin-8 (IL-8) by enzyme-linked immuno sorbent assay after taking supernatants from cocultures and cell elongation induction. For cell elongation quantification, coculture photographs were taken and the proportion of 'hummingbird' cells (>15 μm) was determined. RESULTS: Overall 72% (60/84) of the strains were found to harbor a functional cag PAI. Levels of phos-phorylated CagA were significantly higher for isolates from duodenal ulcer than the ones in strains from gas-tritis, atrophic gastritis, intestinal metaplasia and gastric cancer (49.1% ± 23.1% vs 21.1% ± 19.5%, P < 0.02; 49.1% ± 23.1% vs 26.2%±14.8%, P<0.045; 49.1% ± 23.1% vs 21.5% ± 19.5%, P<0.043 and 49.1% ± 23.1% vs 29.5% ± 27.1%, P < 0.047 respectively). We observed variable IL-8 expression levels ranging from 0 to 810 pg/mL and from 8.8 to 1442 pg/mL at 6 h and 30 h post-infection, respectively. cagPAI-defective strains did not induce detectable levels of IL-8 at 6 h post-infection. At 30 h post-infection all strains induced IL-8 expression in AGS cells, although cagPAI-defective strains induced significantly lower levels of IL-8 than strains with a functional cagPAI (57.1 ± 56.6 pg/mL vs 513.6 ± 338.6 pg/mL,P < 0.0001). We did not observe differences in the extent of cell elongation induction between strains with a functional or a defective cagPAI in 6 h cocultures. At 24 h post infection strains with functionalcagPAI showed high diversity in the extent of hummingbird phenotype induction ranging from 7% to 34%. cag PAI defective strains induced significantly lower levels of elongation than strains with functional cag-PAI with one or more than one EPIYA-C motif (15.1% ± 5.2%vs 18.9% ± 4.7%,P < 0.03; and 15.1% ± 5.2% vs 20.0% ± 5.1%, P < 0.003 respectively). No differences were observed in cellular elongation inductionor IL-8 expression among H. pylori strains bearing one and more than one EPIYA-C motifs, neither at 6 h nor at 24 h of coculture. There were no associations between the levels of induction of cell elongation or IL-8 expression and number of EPIYA motifs or pathology. CONCLUSION: The present work describes a lack of association between H. pylori CagA protein EPIYA motifs variations from Colombian isolates and disease-associated cellular responses. | Carlos Alberto Fajardo Andrés Javier Quiroga Andrea Coronado Karen Labrador Nicole Acosta Pilar Delgado Carlos Jaramillo María Mercedes Bravo | 2013 | World Journal of Gastrointestinal Oncology2013，5，3： | 3 |
| 5 | 幽门螺杆菌EPIYA多态性与胃癌关系的研究进展显示文摘胃癌是世界第二位致死性肿瘤。大量研究表明,胃癌的发生与幽门螺杆菌(Hp)合成分泌的CagA蛋白密切相关。CagA蛋白羧基末端EPIYA重复序列是酪氨酸磷酸化的主要位点,EPIYA的不同基序片段磷酸化后会引起细胞信号转导通路改变,进而影响细胞有丝分裂,引起细胞形态发生改变,最终导致胃黏膜细胞癌变。因此EPIYA基序多态性可能与胃癌发生密切相关,但目前有关这一关系的前瞻性流行病学研究结果存在争议。此文从EPIYA结构的多态性出发,对比不同方法、不同地域的研究,就EPIYA多态性与胃癌发生的关系作一综述。 | 林震 郑建 | 2015 | 国际消化病杂志2015，35，2： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员