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| 1 | 上海地区臭氧周末效应研究显示文摘分析了2006年上海5个臭氧监测站(徐家汇、崇明、宝山、浦东和金山)周末与工作日臭氧浓度的变化规律,发现上海徐家汇与国外许多城市中心一样,存在周末臭氧浓度比工作日高,而臭氧前体物NO,NO2,CO和VOCs的浓度却是周末要比工作日低的“臭氧周末效应”.一方面,上海徐汇区NO2/NO在周末比工作日要高25.61%,NO排放的减少是造成上海臭氧周末效应可能的化学原因;另一方面,上海徐家汇由于周末NOx(NO+NO2)比工作日在清晨(05:00-09:00)平均减少近12.13%,使清晨NO抑制臭氧生成的持续时间比工作日少近半个小时,周末臭氧积累持续时间更长,臭氧平均生成速率更大.臭氧的产生率是关于环境中VOCs与NOx混合比率的函数.上海徐家汇VOCs与NOx比率周末为4.55,工作日为4.37,位于VOC敏感区.由于周末NOx和VOCs减少,VOCs/NOx比率增加,使臭氧从73mL/L增加到80nL/L,这与上海徐家汇的“周末效应”基本一致.利用MICAPS云量资料做进一步分析.周末、工作日臭氧值都随云量增加而降低,并且明显发现徐家汇臭氧“周末效应”随云量增加而逐渐减弱.云量的增加最终结果使臭氧“周末效应”几乎消失,说明徐家汇臭氧“周末效应”是由于臭氧光化学生成引起的. | 唐文苑 赵春生 耿福海 彭丽 周广强 高伟 许建明 Xuexi Tie | 2009 | 中国科学(D辑)2009，39，1： | 103 |
| 2 | Coding metamaterials, digital metamaterials and programmable metamaterials显示文摘Metamaterials are artificial structures that are usually described by effective medium parameters on the macroscopic scale,and these metamaterials are referred to as‘analog metamaterials’.Here,we propose‘digital metamaterials’through two steps.First,we present‘coding metamaterials’that are composed of only two types of unit cells,with 0 and p phase responses,which we name‘0’and‘1’elements,respectively.By coding‘0’and‘1’elements with controlled sequences(i.e.,1-bit coding),we can manipulate electromagnetic(EM)waves and realize different functionalities.The concept of coding metamaterials can be extended from 1-bit coding to 2-bit coding or higher.In 2-bit coding,four types of unit cells,with phase responses of 0,p/2,p,and 3p/2,are required to mimic the‘00’,‘01’,‘10’and‘11’elements,respectively.The 2-bit coding has greater freedom than 1-bit coding for controlling EM waves.Second,we propose a unique metamaterial particle that has either a‘0’or‘1’response controlled by a biased diode.Based on this particle,we present‘digital metamaterials’with unit cells that possess either a‘0’or‘1’state.Using a field-programmable gate array,we realize digital control over the digital metamaterial.By programming different coding sequences,a single digital metamaterial has the ability to manipulate EM waves in different manners,thereby realizing‘programmable metamaterials’.The above concepts and physical phenomena are confirmed through numerical simulations and experiments using metasurfaces. | Tie Jun Cui Mei Qing Qi Xiang Wan Jie Zhao Qiang Cheng | 2014 | Light(Science & Applications)2014，3，1： | 130 |
| 3 | Black carbon aerosols and their radiative properties in the Pearl River Delta region显示文摘The climatic and environmental effects of atmospheric aerosols are a hot topic in global science community, and radiative properties of the aerosols are one of the important parameters in assessing climatic change. Here we studied the black carbon concentration and absorption coefficient measured with aethalometers, scattering coefficient measured with nephelometers, and single scattering albedo derived at an atmospheric composition watch station in Guangzhou from 2004 to 2007. Our main results are as follows. The data of black carbon concentration and absorption coefficients measured with instruments cannot be directly used until they are measured in parallel with internationally accepted instruments for comparison, calibration, and reduction. After evaluation of the data, the result shows that the monthly mean of BC concentration varies 3.1―14.8 μg·m-3 and the concentration decreases by about 1 μg·m-3 in average over the four years; It is higher in the dry season with a multi-year mean of 8.9 μg/m3 and lower in the rainy season with a multi-year mean of 8.0 μg·m-3; The extreme maximum of monthly mean concentration occurred in December 2004 and extreme minimum in July 2007, and a 4-year mean is 8.4 μg·m-3. It is also shown that monthly mean scattering coefficient derived varies 129 -565 Mm-1, monthly mean absorption coefficient 32-139 Mm-1, and monthly mean single scattering albedo 0.71-0.91, with annual mean values of 0.80, 0.82, 0.79 and 0.84 for 2004, 2005, 2006 and 2007, respectively. Three instruments were used to take simultaneous measurements of BC in PM10, PM2.5, and PM1 and the results showed that PM2.5 took up about 90% of PM10 and PM1 accounted for about 68% of PM2.5, and BC aerosols are mainly present in fine particulates. The variability of BC concentrations is quite consistent between the Nancun station (141 m above sea level) and the Panyu station (13 m above sea level), which are 8 km apart from each other. The concentration in higher altitude station (Panyu) is consistently lower than the lower altitude station (Nancun), and the difference of annual mean is about 4 μg·m-3. | WU Dui MAO JieTai DENG XueJiao TIE XueXi ZHANG YuanHang ZENG LiMin LI Fei TAN HaoBo BI XueYan HUANG XiaoYing CHEN Jing DENG Tao | 2009 | Science China Earth Sciences2009，52，8： | 58 |
| 4 | Evolution of planetary boundary layer under different weather conditions,and its impact on aerosol concentrations显示文摘一个领域实验在天津被进行,从 2010 年 9 月 930 日的中国,在表面空气污染物质上集中了于行星的边界层(PBL ) 和它的影响的进化。实验使用了三台遥感仪器,风侧面雷达( WPR ),微波辐射计( MWR )和微脉搏的激光雷达( MPL ),到检测风,温度,和喷雾器 backscattering 系数并且到的垂直侧面测量表面污染物质的垂直侧面(喷雾器,公司, SO2 , NOx ),并且也从 255-m 收集了声音的风速表数据气象学的塔。把大小基于这些, PBL 的进化被估计。平均 PBL 高度在夜间期间在 noon/afternoon-time,和 200300 m 期间是大约 10001300 m。PBL 高度和喷雾器集中是相关反的在期间清楚并且薄雾条件。当平均喷雾器集中分别地是在薄雾和晴朗天空条件下面的 52 和 17 g/m3 时,平均最大的 PBL 高度是 1.08 和 1.70 km。太阳的放射上的喷雾器和云的影响基于从 255-m 收集的声音的风速表数据被观察气象学的塔。当 PBL 的镇压结构进一步削弱了污染物质的散开时,热流动被发现被薄雾(重污染) 或云显著地减少了,它趋于压抑 PBL 的发展,导致重污染。这个可能的积极反馈周期(更多的喷雾器降低 PBL 高度更多的喷雾器) 将在大城市为重地面污染导致一个加速过程。 | Jiannong Quan Yang Gao Qiang Zhang Xuexi Tie Junji Cao Suqin Han Junwang Meng Pengfei Chen Delong Zhao | 2013 | Particuology2013，11，1： | 51 |
| 5 | Aerosol pollution in China:Present and future impact on environment显示文摘With its dense population, rapid economic growth and dramatic rate of urbanization, China is experiencing extreme air pollution problems. This is particularly the case in Central-Eastern China (CEC), where the two major cities of Beijing and Tianjin are located, in the Yangtze-River Delta (YRD) with the city of Shanghai, and in the Pearl-River Delta (PRD) with the mega-city of Guangzhou. Space observations show that the atmospheric aerosol load in these three regions is considerably higher than, for example, in the urbanized regions of Europe and North America. The high aerosol concentrations in these regions have raised many environmental problems, such as impact on human health, visibility, and climate changes. In this paper, several crucial issues regarding aerosol pollution in these highly populated regions (CEC, YRD, and PRD) are discussed, including (1) when the aerosol load starts to rapidly increase in these regions; (2) how the high aerosol concentrations affects the environment; and (3) what the potential consequences are under possible low aerosol load in these regions. Discussion on these crucial issues might lead to some insight for better understanding of the characterizations of aerosol pollution due to the rapid economical development in China. | Xuexi Tie Junji Cao | 2009 | Particuology2009，7，6： | 48 |
| 6 | Identification and expansion of cancer stem cells in tumor tissues and peripheral blood derived from gastric adenocarcinoma patients显示文摘胃的癌症是世界范围的第四很普通的癌症,与死亡的高率和低 5 年的幸存率。迄今为止,有为胃的癌症的有效治疗学的协议的缺乏。最近的研究建议癌症干细胞(CSC ) 为肿瘤开始,侵略,转移,和抵抗负责到 anticancer 治疗。因此,指向胃的 CSC 的治疗是吸引人的。然而,在人的胃的腺癌(GAC ) 的 CSC 没被描述。这里,我们从 GAC 病人在肿瘤纸巾和外部血识别 CSC。从肿瘤纸巾和病人的外部血被孤立的人的 GAC (GCSC ) 的 CSC 带了 CD44 和 CD54 表面标记,当注入了 immunodeficient 老鼠时,高度类似于原来的人的肿瘤的产生肿瘤,在 vitro 区分了进胃的上皮的房间,并且在 vivo 并且在 vitro 自我更新。我们的调查结果建议有效治疗学的协议必须指向 GCSC。从 GAC 病人的循环的 GCSC 的俘获也为为肿瘤转移潜在地负责的一张批评房间人口的鉴定显示出大潜力,并且为早诊断并且胃的癌症的纵的监视提供一个有效协议。 | Tie Chen Kun Yang Jianhua Yu Wentong Meng Dandan Yuan Feng Bi Fang Liu Jie Liu Bing Dai Xinzu Chen Fang Wang Fan Zeng Hong Xu Jiankun Hu Xianming Mo | 2012 | Cell Research2012，22，1： | 50 |
| 7 | Programmable time-domain digital-coding metasurface for non-linear harmonic manipulation and new wireless communication systems显示文摘Optical non-linear phenomena are typically observed in natural materials interacting with light at high intensities, and they benefit a diverse range of applications from communication to sensing. However,controlling harmonic conversion with high efficiency and flexibility remains a major issue in modern optical and radio-frequency systems. Here, we introduce a dynamic time-domain digital-coding metasurface that enables efficient manipulation of spectral harmonic distribution. By dynamically modulating the local phase of the surface reflectivity, we achieve accurate control of different harmonics in a highly programmable and dynamic fashion, enabling unusual responses, such as velocity illusion. As a relevant application, we propose and realize a novel architecture for wireless communication systems based on the time-domain digital-coding metasurface, which largely simplifies the architecture of modern communication systems, at the same time yielding excellent performance for real-time signal transmission. The presented work, from new concept to new system, opens new pathways in the application of metamaterials to practical technology. | Jie Zhao Xi Yang Jun Yan Dai Qiang Cheng Xiang Li Ning Hua Qi Jun Chen Ke Guo Dong Bai Shuo Liu Shi Jin Andrea Alù Tie Jun Cui | 2019 | National Science Review2019，6，2： | 46 |
| 8 | Major complications after radiofrequency ablation for liver tumors:Analysis of 255 patients显示文摘AIM:To investigate the major complications after radiofrequency ablation(RFA) for the treatment of liver tumors and analyze possible risk factors that precipitate these complications.METHODS:From March 2001 to April 2008,255 patients with liver tumors(205 male,50 female;age range,18-89 years;mean age,56.0 years) who received RFA were enrolled in this study.Of these patients,212 had hepatocellular carcinoma,39 had metastatic liver tumors and four had cholangiocellular carcinoma.One hundred and forty eight patients had a single tumor,and 107 had multiple tumors.Maximum diameter of the tumors ranged 1.3-20 cm(mean,5.1 cm).All patients were treated with a cooled-tip perfusion electrode attached to a radiofrequency generator(Radionics,Burlington,MA,USA).RFA was performed via the percutaneous approach(n = 257),laparoscopy(n = 7),or open surgical treatment(n = 86).The major complications related to RFA were recorded.The resultant data were analyzed to determine risk factors associated these complications.RESULTS:Among the 255 patients,425 liver tumors were treated and 350 RFA sessions were performed.Thirty-seven(10%) major complications were observed which included 13 cases of liver failure,10 cases of hydrothorax requiring drainage,three casesof tumor seeding,one case of upper gastrointestinal bleeding,one case ofintrahepatic abscess,one case of bile duct injury,one case of cardiac arrest,and five cases of hyperglycemia.Seven patients had more than two complications.Liver failure was the most severe complication and was associated with the highest mortality.Eleven patients died due to worsening liver decompensation.Child-Pugh classification(P = 0.001) and choice of approach(P = 0.045) were related to post-treatment liver failure,whereas patient age,tumor size and number were not significant factors precipitating this complication.CONCLUSION:RFA can be accepted as a relatively safe procedure for the treatment of liver tumors.However,attention should be paid to possible complications even though the incidences of these complications are rare.Careful patient selection and the best approach choice(percutaneous,laparoscopy,or laparotomy) will help to minimize the incidence and morbidity rate of complications which occur after RFA. | Wen-Tao Kong Wei-Wei Zhang Yu-Dong Qiu Tie Zhou Jun-Lan Qiu Wei Zhang Yi-Tao Ding | 2009 | World Journal of Gastroenterology2009，15，21： | 35 |
| 9 | 基于正交拉丁方的数字图像置乱方法显示文摘以图象信息安全问题为背景 ,介绍了正交拉丁方用于图像置乱的理论基础 。 | 李国富 Tie Xiaoyun | 2001 | 北方工业大学学报2001，13，1： | 39 |
| 10 | Anisotropic coding metamaterials and their powerful manipulation of differently polarized terahertz waves显示文摘Metamaterials based on effective media can be used to produce a number of unusual physical properties(for example,negative refraction and invisibility cloaking)because they can be tailored with effective medium parameters that do not occur in nature.Recently,the use of coding metamaterials has been suggested for the control of electromagnetic waves through the design of coding sequences using digital elements‘0’and‘1,'which possess opposite phase responses.Here we propose the concept of an anisotropic coding metamaterial in which the coding behaviors in different directions are dependent on the polarization status of the electromagnetic waves.We experimentally demonstrate an ultrathin and flexible polarization-controlled anisotropic coding metasurface that functions in the terahertz regime using specially designed coding elements.By encoding the elements with elaborately designed coding sequences(both 1-bit and 2-bit sequences),the x-and y-polarized waves can be anomalously reflected or independently diffused in three dimensions.The simulated far-field scattering patterns and near-field distributions are presented to illustrate the dual-functional performance of the encoded metasurface,and the results are consistent with the measured results.We further demonstrate the ability of the anisotropic coding metasurfaces to generate a beam splitter and realize simultaneous anomalous reflections and polarization conversions,thus providing powerful control of differently polarized electromagnetic waves.The proposed method enables versatile beam behaviors under orthogonal polarizations using a single metasurface and has the potential for use in the development of interesting terahertz devices. | Shuo Liu Tie Jun Cui Quan Xu Di Bao Liangliang Du Xiang Wan Wen Xuan Tang Chunmei Ouyang Xiao Yang Zhou Hao Yuan Hui Feng Ma Wei Xiang Jiang Jiaguang Han Weili Zhang Qiang Cheng | 2016 | Light(Science & Applications)2016，5，1： | 36 |
| 11 | Osteogenic effects of D(+)β-3,4-dihydroxyphenyl lactic acid (salvianic acid A, SAA) on osteoblasts and bone marrow stromal cells of intact and prednisone-treated rats显示文摘 | Liao CUI Yu-yu LIU Tie WU Chun-mei AI Huai-qing CHEN | 2009 | Acta Pharmacologica Sinica2009，30，3： | 29 |
| 12 | Independent control of harmonic amplitudes and phases via a time-domain digital coding metasurface显示文摘Harmonic manipulations are important for applications such as wireless communications,radar detection and biological monitoring.A general approach to tailor the harmonics involves the use of additional amplifiers and phase shifters for the precise control of harmonic amplitudes and phases after the mixing process;however,this approach leads to issues of high cost and system integration.Metasurfaces composed of a periodic array of subwavelength resonators provide additional degrees of freedom to realize customized responses to incident light and highlight the possibility for nonlinear control by taking advantage of time-domain properties.Here,we designed and experimentally characterized a reflective time-domain digital coding metasurface,with independent control of the harmonic amplitude and phase.As the reflection coefficient is dynamically modulated in a predefined way,a large conversion rate is observed from the carrier signal to the harmonic components,with magnitudes and phases that can be accurately and separately engineered.In addition,by encoding the reflection phases of the meta-atoms,beam scanning for multiple harmonics can be implemented via different digital coding sequences,thus removing the need for intricate phase-shift networks.This work paves the way for efficient harmonic control for applications in communications,radar,and related areas. | Jun Yan Dai Jie Zhao Qiang Cheng Tie Jun Cui | 2018 | Light(Science & Applications)2018，7，1： | 29 |
| 13 | 利用原生质体融合技术选育微生物菌种显示文摘对原生质体融合技术的遗传标记筛选、原生质体的制备与再生、原生质体融合等的方法 ,以及原生质体融合技术在提高抗生素生产效价、酵母菌工程菌株的构建、多功能菌株的选育以及污水处理工程菌的构建等方面的研究情况进行了综述。就原生质体融合技术在微生物育种技术上的应用前景进行了分析。 | 谭周进 杨海君 林曙 吴铁 WU Tie | 2005 | 核农学报2005，19，1： | 28 |
| 14 | Study of ozone “weekend effect” in Shanghai显示文摘Analysis of observed ozone data in 2006 from five monitoring sites (Xujiahui, Chongming, Baoshan, Pudong, Jinshan) in Shanghai reveals that ozone (O_3) concentrations in Xujiahui are higher at week-ends than those on weekdays, despite the fact that emissions of ozone precursor substances, such as oxides of nitrogen (NOx), carbon monoxide (CO) and volatile organic compounds (VOCs) are lower at weekends than those on weekdays. The possible chemical cause of ozone 'weekend effect' is that NO_2/NO ratio increases at weekends by 25.61% compared with those on weekdays. In addition, because of an average 12.13% reduction in NOx (NO + NO_2) in the early morning (05:00-09:00) at weekends compared with that on weekdays, the ozone inhibition period ends 0.5 h earlier at weekends resulting in the longer duration of ozone accumulation and the higher ozone production rate. The rate of ozone production is a function of VOCs and NOx in the atmosphere. VOCs/NOx ratio in Xujiahui is 4.55 at weekends, and 4.37 on weekdays, belonging to the 'NOx-limited'. The increasing VOCs/NOx ratio at weekends leads to ozone enhancement from 73 ppbv to 80 ppbv, which are consistent with ozone 'weekend effect' in Xujiahui. Furthermore, combining with MICAPS cloud amount data, the fact that ozone 'weekend effect' in Xujiahui weakens gradually along with the increasing of cloud amount indicates that ozone photochemical production leads to ozone 'weekend effect' in Xujiahui of Shanghai. | TANG WenYuan1↑, ZHAO ChunSheng1, GENG FuHai1,2, PENG Li2, ZHOU GuangQiang2, GAO Wei2, XU JianMing2 & TIE XueXi2,3 1 Department of Atmospheric Science, School of Physics, Peking University, Beijing 100871, China 2 Atmospheric Chemistry Laboratory of Shanghai Meteorological Bureau, Shanghai 200030, China 3 Atmospheric Chemistry Division, NCAR, Boulder CO 80301, USA | 2008 | Science China Earth Sciences2008，51，9： | 23 |
| 15 | A Review on Grain Refinement of Aluminum Alloys:Progresses,Challenges and Prospects显示文摘Aluminum becomes the most popular nonferrous metal and is widely used in many fields such as packaging,building transportation and electrical materials due to its rich resource, light weight, good mechanical properties, suitable corrosion resistance and excellent electrical conductivity. Grain refinement, which is obtained by changing the size of grain structure by different techniques, is a preferred method to improve simultaneously the strength and plasticity of metallic materials. Therefore, grain refining of aluminum is regarded as a key technique in aluminum processing industry.Up to now, there have been a number of techniques for aluminum grain refining. All the techniques can be classified as four categories as follows: grain refining by vibration and stirring during solidification, rapid solidification, the addition of grain refiner and severe plastic deformation. Each of them has its own merits and demerits as well as applicable conditions, and there are still some arguments in the understanding of the mechanisms of these techniques. In this article, the research progresses and challenges encountered in the present techniques and the future research issues and directions are summarized. | Ren-Guo Guan Di Tie | 2017 | Acta Metallurgica Sinica(English Letters)2017，30，5： | 23 |
| 16 | Wireless Communications with Programmable Metasurface:Transceiver Design and Experimental Results显示文摘Metasurfaces have drawn significant attentions due to their superior capability in tailoring electromagnetic waves with a wide frequency range, from microwave to visible light. Recently, programmable metasurfaces have demonstrated the ability of manipulating the amplitude or phase of electromagnetic waves in a programmable manner in real time, which renders them especially appealing in the applications of wireless communications. In this paper, we present the fundamental principle of applying programmable metasurface as transmitter for wireless communications. Then, we establish a prototype system of metasurface-based transmitter to conduct several experiments and measurements over the air, which practically demonstrate the feasibility of using programmable metasurfaces in future communication systems. By exploiting the dynamically controllable property of programmable metasurface, the design, implementation and experimental evaluation of the proposed metasurface-based wireless communication system are presented with the prototype, which realizes single carrier quadrature phase shift keying(QPSK) transmission over the air. In the developed prototype, the phase of the reflected electromagnetic wave of programma-ble metasurface is directly manipulated in real time according to the baseband control signal, which achieves 2.048 Mbps data transfer rate with video streaming transmission over the air. In addition, experimental result is provided to compare the performance of the proposed metasurface-based architecture against the conventional one. With the slight increase of the transmit power by 5 dB, the same bit error rate(BER) performance can be achieved as the conventional system in the absence of channel coding. Such a result is encouraging considering that the metasurface-based system has the advantages of low hardware cost and simple structure, thus leading to a promising new architecture for wireless communications. | Wankai Tang Xiang Li Jun Yan Dai Shi Jin Yong Zeng Qiang Cheng Tie Jun Cui | 2019 | China Communications2019，16，5： | 23 |
| 17 | Direct Transmission of Digital Message via Programmable Coding Metasurface显示文摘In modern wireless communications,digital information is frstly converted to analog signal by a digital-analog convertor,which is then mixed to high-frequency microwave to be transmitted through a series of devices including modulator,mixer,amplifer,flter,and antenna and is fnally received by terminals via a reversed process.Although the wireless communication systems have evolved signifcantly over the past thirty years,the basic architecture has not been challenged.Here,we propose a method to transmit digital information directly via programmable coding metasurface.Since the coding metasurface is composed of‘0’and‘1’digital units with opposite phase responses,the digital information can be directly modulated to the metasurface with certain coding sequences and sent to space under the illumination of feeding antenna.Te information,being modulated in radiation patterns of the metasurface,can be correctly received by multiple receivers distributed in diferent locations.Tis method provides a completely new architecture for wireless communications without using complicated digital-analog convertor and a series of active/passive microwave devices.We build up a prototype to validate the new architecture experimentally,which may fnd promising applications where information security is highly demanded. | Tie Jun Cui Shuo Liu Guo Dong Bai Qian Ma | 2019 | Research2019，，1： | 20 |
| 18 | APPLYING PPE MODEL BASED ON RAGA TO CLASSIFYAND EVALUATE SOIL GRADE显示文摘The research of soil classification and soil grade evaluation is often based on fuzzy theory. So, the traditional method has an inevitable problem about weight matrix which given by some experts, and the final result can be influenced by artificial factors. The essentials of fuzzy synthetically judge is to handle the data of high dimension. That is to reducing the dimension number. The weight matrix in fuzzy theory is corresponding to low dimension projection value of each index. But we can′t define whether the weight matrix given by experts is the best projection value or not. So, the authors apply a new technique of falling dimension named projection pursuit to soil study, through using the improved real coding based accelerating genetic algorithm to optimize the projection direction. Thus, it can transfer multi dimension data into one dimension data, through searching for the optimum projection direction to realize the soil classification and its grade evaluation. The method can avoid the artificial disturbance, and acquire preferably effect. Thus, the paper provides a new method to the research of soil classification and grade evaluation. | FU Qiang1,2, LU Tie guang2, FU Hong2(1. College of Hydraulic Power, Sichuan University, Chengdu 610065, P.R.China 2. Northeast Agricultural University, Harbin 150030, P. R. China) | 2002 | Chinese Geographical Science2002，12，2： | 19 |
| 19 | Transurethral seminal vesiculoscopy for recurrent hemospermia: experience from 419 cases显示文摘 | Rui Chen Lei Wang Xia Sheng Shu-Guang Piao Xin-Wen Nian Xin Cheng Tie Zhou Hui-Zhen Li Ya-Wei Liu Guang-Hua Chen Chun-Lei Zhang De-Pei Kong Guang-An Xiao Xin Lu Zhen-Yu Jia Zhi-Yong Liu Ying-Hao Sun | 2018 | Asian Journal of Andrology2018，20，5： | 18 |
| 20 | Suppression of matrix metalloproteinase-2 via RNA interference inhibits pancreatic carcinoma cell invasiveness and adhesion显示文摘AIM:To investigate the inhibitory effects of RNA interference (RNAi) on expression of matrix metalloproteinase-2 (MMP-2) gene and invasiveness and adhesion of human pancreatic cancer cell line,BxPC-3.METHODS:RNAi was performed using the vector (pGPU6)-based small interference RNA (siRNA) plasmid gene silence system to specifically knock down MMP-2 expression in pancreatic cancer cell line,BxPC-3. Four groups of different specific target sequence in coding region of MMP-2 and one non-specific sequence were chosen to construct four experimental siRNA plasmids of pGPU6-1,pGPU6-2,pGPU6-3 and pGPU6-4,and one negative control siRNA plasmid of pGPU6 (-). MMP-2 expression was measured by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Cell proliferation and apoptosis were examined by methyl thiazolyl tetrazolium (MTT) and flow cytometry,respectively. The abilities of adhesion and invasion were detected by cell adhesion assay and cell invasion assay using Transwell chambers.RESULTS:The expression of MMP-2 was inhibited and the inhibitory effects of different sequence varied. pGPU6-1 group had the most efficient inhibitory effect,followed by pGPU6-2 and pGPU6-3 groups.Invasiveness and adhesion were more significantly reduced in pGPU6-1,pGPU6-2 and pGPU6-3 groups as compared with pGPU6 (-) and blank control groups. However,no difference concerning cell proliferation and apoptosis was observed after transfection between experiment groups and control groups.CONCLUSION:RNAi against MMP-2 successfully inhibited the mRNA and protein expression of MMP-2 in the pancreatic cancer cell line,BxPC-3,leading to a potent suppression of tumor cell adhesion and invasion without affecting cell proliferation and apoptosis. These findings suggest that the RNAi approach towards MMP-2 may be an effective therapeutic strategy for the clinical management of pancreatic tumor. | Ying-Hui Zhi Mao-Min Song Pi-Lin Wang Tie Zhang Zi-Yi Yin | 2009 | World Journal of Gastroenterology2009，15，9： | 16 |
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