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| 1 | Ultrafast photonic PCR显示文摘Nucleic acid amplification and quantification via polymerase chain reaction(PCR)is one of the most sensitive and powerful tools for clinical laboratories,precision medicine,personalized medicine,agricultural science,forensic science and environmental science.Ultrafast multiplex PCR,characterized by low power consumption,compact size and simple operation,is ideal for timely diagnosis at the point-of-care(POC).Although several fast/ultrafast PCR methods have been proposed,the use of a simple and robust PCR thermal cycler remains challenging for POC testing.Here,we present an ultrafast photonic PCR method using plasmonic photothermal light-to-heat conversion via photon–electron–phonon coupling.We demonstrate an efficient photonic heat converter using a thin gold(Au)film due to its plasmon-assisted high optical absorption(approximately 65%at 450 nm,the peak wavelength of heat source light-emitting diodes(LEDs)).The plasmon-excited Au film is capable of rapidly heating the surrounding solution to over 150℃ within 3 min.Using this method,ultrafast thermal cycling(30 cycles;heating and cooling rate of 12.7960.93℃ s^(-1) and 6.660.29℃ s^(-1),respectively)from 55℃(temperature of annealing)to 95℃(temperature of denaturation)is accomplished within 5 min.Using photonic PCR thermal cycles,we demonstrate here successful nucleic acid(λ-DNA)amplification.Our simple,robust and low cost approach to ultrafast PCR using an efficient photonic-based heating procedure could be generally integrated into a variety of devices or procedures,including on-chip thermal lysis and heating for isothermal amplifications. | Jun Ho Son Byungrae Cho SoonGweon Hong Sang Hun Lee Ori Hoxha Amanda J Haack Luke P Lee | 2015 | Light(Science & Applications)2015，4，1： | 2 |
| 2 | Impact of baemoglobin- variabiIity in haemodialysis patients receiving erythropoie- sis stimulating agents for the management of renal anaemi- a 显示文摘 | Mac dougall IC son P Roche A | 2005 | J Am Soc Nephrol2005，16，： | 1 |
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| 4 | An integrated hy- pothesis that considers drusen as biomarkers of immune- mediated processes at the RPE-Bruch' s membrane inter- face in aging and age-related macular degeneration显示文摘 | HAGEMAN GS LUTHERT P J VICTOR CHONG NH JOHN- SON LV ANDERSON DH MULLINS RF | 2001 | Prog Retin Eye Res2001，20，6： | 1 |
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| 7 | Generalized inverses of a 2 × 2 block partitioned matrix显示文摘 | Xu H W Son L P Wang G R | 2007 | Journal of Shanghai Normal University2007，36，3： | 1 |
| 8 | Sex attractants for moths of Vietnam: Field attraction by synthetic lures baited with known lep- idopteran pheromones 显示文摘 | Hai T V Vang L V Son P K | 2002 | J Chem Ecol2002，28，： | 1 |
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| 10 | Numerical simulation of sleeve repair welding of in-service gas pipelines显示文摘 | Bang I W Son Y P Oh K H | 2002 | Weld- ing Journal2002，12，： | 1 |
| 11 | Quereetin inhibits angiogenesis mediated human prostate tumor growth by targeting VEGFR-2 regulated AKT/mTOR/P70S6K signaling pathways 显示文摘 | Pratheeshkumar P Budhraja A Son YO | 2012 | PLoS One2012，7，47： | 1 |
| 12 | Progress and challen- ges in translating the biology of atherosclerosis 显示文摘 | Libby P Ridker PM Hans son GK | 2011 | Na- ture2011，473，7347： | 1 |
| 13 | Long- term changes in arch form after orthodontic treament and retention显示文摘 | De La CruzA Samp son P Little R | 1995 | Am J Orthod Dentofaeial Orthop1995，107，5： | 1 |
| 14 | Oxidative stress and metal carcinogenesis 显示文摘 | Lee JC Son YO Pratheeshkumar P | 2012 | Free Radic Biol Med2012，53，4： | 1 |
| 15 | Luteo- lin inhibits human prostate tumor growth by suppressing vascular endothelial growth factor receptor 2 - mediated angiogenesis 显示文摘 | Pratheeshkumar P Son Y O Budhraja A | 2012 | Plos One2012，7，52： | 1 |
| 16 | Role of vascular endothelial growth factor and placental growth factors during retinal vascular devel- opment and hyaloid regression 显示文摘 | FEENEY SA SIMPSON DA GARDINER TA BOYLE C JAMI- SON P STITI' AW | 2003 | Invest Ophthalmol Vis Sci2003，44，2： | 1 |
| 17 | Evaluation of candidate vaccine approaches for mers-cov显示文摘 | Wang L Shi W Joyce M G Modjarrad K Zhang Y Leung K Lees C R Zhou T Yassine H M KanekiyoM Yang Z Y Chen X Becker M M Freeman M Vogel L Johnson J C Olinger G Todd J P Bagci U Solomon J Mollura D J Hensley L Jahrling P Deni- son M R Rao S S Subbarao K Kwong P D Mascola J R Kong W P Graham B S | 2015 | Nat Commun2015，6，： | 1 |
| 18 | Patients admitted to the emergency room with symptoms indicative of acute myocardial infarction显示文摘 | Karl son BW Herlitz J Pettersson P | 1991 | J Intern Med1991，230，： | 1 |
| 19 | Prominent Defects in Semi-insulating SiC Substrates 显示文摘 | SON N T CARLSSON P GALLSTROM A | 2007 | Physica B2007，，1： | 1 |
| 20 | Aaptation of the MIMICS Backscattering Model to the Agricultural Context - wheat and Canola at L and C Bands显示文摘 | Toure A Thom son K P B Edwards G | 1994 | IEEE Trans on G&RS1994，32，： | 1 |
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