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| 1 | Manipulation of spermatogonial stem cells in livestock species显示文摘We are entering an exciting epoch in livestock biotechnology during which the fundamental approaches(such as transgenesis, spermatozoa cryopreservation and artificial insemination) will be enhanced based on the modern understanding of the biology of spermatogonial stem cells(SSCs) combined with the outstanding recent advances in genomic editing technologies and in vitro cell culture systems. The general aim of this review is to outline comprehensively the promising applications of SSC manipulation that could in the nearest future find practical application in livestock breeding. Here, we will focus on 1) the basics of mammalian SSC biology;2) the approaches for SSC isolation and purification;3) the available in vitro systems for the stable expansion of isolated SSCs;4) a discussion of how the manipulation of SSCs can accelerate livestock transgenesis;5) a thorough overview of the techniques of SSC transplantation in livestock species(including the preparation of recipients for SSC transplantation,the ultrasonographic-guided SSC transplantation technique in large farm animals, and the perspectives to improve further the SSC transplantation efficiency), and finally, 6) why SSC transplantation is valuable to extend the techniques of spermatozoa cryopreservation and/or artificial insemination. For situations where no reliable data have yet been obtained for a particular livestock species, we will rely on the data obtained from studies conducted in rodents because the knowledge gained from rodent research is translatable to livestock species to a great extent. On the other hand, we will draw special attention to situations where such translation is not possible. | Filipp Savvulidi Martin Ptacek Karina Savvulidi Vargova Ludek Stadnik | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 6 |
| 2 | In- situ remediation of chromate contaminated groundwater using permeable reactive walls显示文摘 | Blowes D W Ptacek C J Jambor J L | 1997 | Environ Sci Technol1997，31，： | 1 |
| 3 | Sediment as a source of nutrients to hypereutropic marshes of Point Pelee,Ontario,Canada显示文摘 | MAYER T PTACEK C ZANINI L | 1999 | Water Research1999，33，6： | 1 |
| 4 | Determination of finasteride in human plasma by liquid-liquid extraction and high-performance liquid chromatography显示文摘 | Macek J Klimat | 2000 | J Chromatogr B Biomed Sci Appl2000，738，2： | 1 |
| 5 | Rapid determination of citalopram in human plasma by high - performance liquid chromatography显示文摘 | Macek J Ptacek P Klima J | 2001 | MJ Chromatogr B Biomed Sci Appl2001，755，12： | 1 |
| 6 | Determination of finaste-ride in human plasma by liquid- liquid extraction and high- performance liquid chromatography显示文摘 | Ptacek P Macek J Klima J | 2000 | J Chromatogr B Biomed Sci Appl2000，738，2： | 1 |
| 7 | Rates of sulfate reduction and metal sulfide precipitation in a permeable reactive barrier显示文摘 | Benner S G Blowes D W Ptacek C J | 2002 | Appl Geochem2002，17，： | 1 |
| 8 | Long-term perfo- mance of in situ reactive barriers for nitrate remediation 显示文摘 | Robertson W D Blowes D W Ptacek C J | 2000 | Ground- water2000，38，5： | 1 |
| 9 | Dilhydropyridine receptor mutations cause hypokalemic periodic paralysis显示文摘 | Ptacek LJ Tawil R Griggs RC | 1994 | Cell1994，77，6： | 1 |
| 10 | Rapid determination of pseudoephedrine in human plasma by high-performance liquid chromatography 显示文摘 | MAC EK J PTACEK P KLIMA J | 2002 | J Chromatogr B2002，766，2： | 1 |
| 11 | In-situ remediation of Cr(VI)-contaminated groundwater using permeable reactive walls: Laboratory studies显示文摘 | Blowes D W Ptacek C J Jambor J L | 1997 | Environ Sci Technol1997，31，12： | 1 |
| 12 | Microbial and nutrient investigations into the use of in situ layers for treatment of tailings effluent 显示文摘 | Hulshof A H M Blowes D W Ptacek C J | 2003 | Environmental Science and Technology2003，37，21： | 1 |
| 13 | Geochemical and Hydrogeological Impacts of a Wood Particle Barrier TreatingNitrate and Perchlorate in Ground Water显示文摘 | Robertson W D Ptacek C J Brown S J | 2007 | Ground Water Monitoring & Remediation2007，27，2： | 1 |
| 14 | Determination of alendronate in human urine as 9-fluorenylmethyl derivative by high-performance liquid chromatography 显示文摘 | Ptacek P Klima J Macek J | 2002 | J Chromatogr B Analyt Technol Biomed Life Sci2002，767，1： | 1 |
| 15 | Long-term performance of in situ reactive barriers for nitrate remedia- tion显示文摘 | Robertson W D Blowes D W Ptacek C J | 2000 | Ground Water2000，38，5： | 1 |
| 16 | Numerical modelling of groundwater flow and contaminant transport to Point Pelee marsh, Ontario, Canada 显示文摘 | CROWE A S SHIKAZE S G PTACEK C J | 2004 | Hydrological Processes2004，2，18： | 1 |
| 17 | Selection of reactive mixtures for the prevention of acid mine drainage using in situ porous reactive walls显示文摘 | Waybrant K R Blowes D W Ptacek C J | 1995 | Mining and the Environment1995，3，： | 1 |
| 18 | Rapid determination of citalopram in human plasma by high-performance liquid chromatography显示文摘 | Macek J Ptacek P Klima J | 2001 | MJ Chromatogr B Biomed Sci Appl2001，755，12： | 1 |
| 19 | Determination of cetirizine in human plasma by high-performance liquid chromatography显示文摘 | MACEK J PTACEK P KLIMA J | 1999 | Journal of Chromatography B1999，736，： | 1 |
| 20 | Protein microarray technology 显示文摘 | HALL D A PTACEK J SNYDER M | 2007 | Mech Ageing Dev2007，128，1： | 1 |
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