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| 1 | Signaling transduction pathways involved in basophil adhesion and histamine release显示文摘Background Little is known about basophil with respect to the different signaling transduction pathways involved in spontaneous, cytokine or anti-IgE induced adhesion and how this compares to IgE-dependent and IgE-independent mediator secretion. The purpose of the present study was to investigate the roles of β1 andβ2 integrins in basophil adhesion as well as hosphatidylinositol 3-kinase (PI3K), src-kinases and extracellular signal regulated kinase (ERK)1/2 in basophil adhesion and histamine release (HR). Methods Basophils (purity of 10%-50%) were preincubated with anti-CD29 or anti-CD18 blocking antibodies before used for adhesion study. Basophils were preincubated with the pharmacological inhibitors wortmannin, PP1, PD98059 before used for adhesion and HR study. Cell adherence to bovine serum albumin (BSA) or fibronectin (Fn) was monitored using cell associated histamine as a basophil marker and the histamine was measured by the glass fiber assay. Results Basophil spontaneous adhesion to Fn was inhibited by anti-CD29. Interleukin (IL)-3, granulocyte/macrophage colony stimulating factor (GM-CSF) induced adhesion to BSA was inhibited by anti-CD18. Wortmannin at 1 μmol/L and PP1 at 20 μmol/L strongly interfered with, whereas PD98059 at 50 μmol/L weakly inhibited basophil spontaneous adhesion to Fn. One μmol/L wortmannin strongly inhibited IL-3, IL-5, GM-CSF and anti-IgE induced adhesion to BSA. PP1 at 20 μmol/L partly inhibited anti-IgE induced adhesion. Fifty μmol/L PD98059 marginally inhibited IL-5, weakly inhibited anti-IgE, partly inhibited GM-CSF induced adhesion. Wortmannin, PP1 and PD98059 inhibited anti-IgE (1:100 or 1:1000) induced basophil HR in a dose dependent manner. They inhibited calcium ionophore A23187 (10 μmol/L, 5 μmol/L) induced basophil HR in a dose dependent manner, but to different extend with PP1 being the most efficient. Conclusions Basophil spontaneous adhesion to Fn is mediated by β1-integrins whereas cytokine induced adhesion to BSA is mediated by β2-integrins. PI3K, src-kinases and ERK1/2 play distinct signaling roles in basophil adhesion and HR. PI3K is the key player whileERK1/2 is the weakest participant. | SHA Quan Lars K. Poulsen Jens Gerwien NielsФdum Per Stahl Skov | 2006 | Chinese Medical Journal2006，，2： | 8 |
| 2 | DNA polymorphism of HLA class II genes in primary biliary cirrhosis显示文摘 | Niels Morling Kim Dalhoff Lars Fugger J?rgen Georgsen Bodil Jakobsen Leo Ranek Niels ?dum Arne Svejgaard | 1992 | Immunogenetics1992，，2： | 2 |
| 3 | Comparing the BDI-II and the PHQ-9 without patient substance abusers 显示文摘 | Dum M Pickren J Sobell MB | 2008 | Addict Behav2008，33，2： | 1 |
| 4 | A model of periodontitis in the rat effect of lipopolysaccharide on bone resorption, osteoclast activity, and local peptidrgic innervation 显示文摘 | Dum ltrescu AL Abd-EI-Aleem S Morales-Aza B | 2004 | J Clin Periodontol2004，31，8： | 1 |
| 5 | A simple and convenient microtiter plate assay for the detection of bactericidal antibodies to Vibrio cholerae O1 and Vibrio cholerae O139显示文摘 | Boutonnier A Dassy B Duménil R | 2003 | J Microbiol Methods2003，55，3： | 1 |
| 6 | Giant cell tumor of the thoracic spine: case report and review of the literature显示文摘 | Refai D Dum G Pv Santiago P | 2009 | Surg Neurol2009，71，2： | 1 |
| 7 | Thrombopoietin Induces Histidine Decarboxylase Gene Expression in c-mpl Transfected UT7 Cells显示文摘 | Maria Pacilio Najet Debili Anne Arnould Fran?ois Machavoine Malvyne Rolli-Derkinderen Michael Bodger Michel Arock Dominique Duménil Michel Dy Elke Schneider | 2001 | Biochemical and Biophysical Research Communications2001，，5： | 1 |
| 8 | Lowe-temperature responsive burley genes have different control mechanisms显示文摘 | Dum M A | 1994 | Plant Mol Biol1994，24，： | 1 |
| 9 | Prevalence and significance ofprostatic inflammution显示文摘 | Schaeffer AJ Wendel EF Dum JK et a1 | 1981 | J Urol1981，，： | 1 |
| 10 | The basal ganglia communicate with the cerebellum显示文摘 | Bostan AC Dum RP Strick PL | 2010 | Proc Natl Acad Sci USA2010，107，18： | 1 |
| 11 | Mechanisms of plasma enhanced silicon nitride deposition using SiH4/ N2 mixture显示文摘 | DUM H PAN P WHITE F R | 1981 | J Electrochem Soc1981，128，7： | 1 |
| 12 | Calculation of acoustic impendence of multi-layer absorber显示文摘 | Dum I P Daven W A | 1986 | Applied Acoustics1986，，19： | 1 |
| 13 | Prospective evaluation of health- related quality of life in patients with deep venous thrombosis显示文摘 | Kahn SR Dum'uet T Lamping DL | 2005 | Arch Intern Med2005，92，165： | 1 |
| 14 | Characterizationand evaluation of carbon nanotube bucky-paper membranes fordirectcontact membrane distillation显示文摘 | Ludovic F Dumée Kallista Sears Jürg Schütz | 2010 | Journal of MembraneScience2010，351，： | 1 |
| 15 | The origin of corticospinal projections from the premotor areas in the frontal lobe显示文摘 | Dum RP Strick PL | 1991 | Journal of Neuroscience1991，11，3： | 1 |
| 16 | Removal of metal ions from aqueous solution on low cost natural polysaccharides sorption mechanism approach显示文摘 | Claire G Pierre C duM Yves A etal | 2000 | React Funct Polym2000，46，2： | 1 |
| 17 | A study abrasive wear under three-body conditions显示文摘 | Rabinowicz E Dum L A Rusell P G | 1961 | Wear1961，4，： | 1 |
| 18 | Research on some metabolic and enzymatic modification in experimental hypertensive myocardium 显示文摘 | Balta N Dum itriu IF Stoian G | 1995 | Rom J Physiol1995，32，14： | 1 |
| 19 | Beyond the Transformation Riddle: A Labor Theory of Value显示文摘 | Duménil Gérard | 1983 | Science and Society1983，47，4： | 1 |
| 20 | McCune - Albright syndrome显示文摘 | Dum itrescu CE Collins MT | 2008 | Or-phanet J Rare Dis2008，3，5： | 1 |
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