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| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | Up-regulation of PIK3CA promotes metastasis in gastric carcinoma显示文摘AIM:To explore expressions of PIK3CA in the progression of gastric cancer from primary to metastasis and its effects on activation of phosphatidylinositol 3-kinase(PI3K)/Akt pathway.METHODS:mRNA and protein levels of PIK3CA were assessed,respectively,by real-time quantitative polymerase chain reaction and immunohistochemistry in specimens of normal gastric mucosa,primary foci and lymph node and distant metastasis of gastric cancer.Akt and phosphorylated Akt protein were also examined by Western blotting in these tissues,in order to analyze the effect of PIK3CA expression level changes on the activation of PI3K/Akt signaling pathway.RESULTS:PIK3CA mRNA in lymph node metastasis were approximately 5 and 2 folds higher,respectively,than that in the corresponding normal gastric mucosa and primary gastric cancer tissues(P<0.05),while no statistical significance was found compared with distant metastasis.Immunohistochemically,PIK3CA protein expression was discovered in 7(35%)specimens of 20 primary foci vs 10(67%)of 15 of lymph node metastasis or 11(61%)of 18 of distant metastasis(35%vs 67%,P=0.015;35%vs 61%,P=0.044).With the increased level of PIK3CA expression,the total Akt protein expression remained almost unchanged,but p-Akt protein was upregulated markedly.CONCLUSION:Increased expression of PIK3CA is expected to be a promising indicator of metastasis in gastric cancer.Up-regulation of PIK3CA may promote the metastasis of gastric cancer through aberrant activation of PI3K/Akt signaling. | Liu JF Zhou XK Chen JH Yi G Chen HG Ba MC Lin SQ Qi YC. | 2010 | World Journal of Gastroenterology2010，16，39： | 20 |
| 3 | Expression of perforin and granzyme B mRNA in judgement of immunosuppressive effect in rat liver transplantation显示文摘AIM To explore the expression of perform andgranzyme B genes mRNA to judge the effect ofimmunosuppression in acute rejection of livertransplantation.METHODS The expression of perform andgranzyme B genes mRNA was examined byreverse transcriptase-polymerase chain reaction(RT--PCR) in hamster to rat liver grafts under theimmunosuppression of cyclosporine or/andsplenectomy. Histological findings were studiedcomparatively.RESULTS Cyclosporine could obviouslydecrease the cellular infiltration, and completelyrepress the expression of mRNA for perform andgranzyme B, but could not change severehepatocyte necrosis and hemorrhage.SPlenectomy could significantly lightenhepatocyte necrosis, and completely eliminatehemorrhage, but not atfect the cellularinfiltration and the expression of perform andgranzyme B genes mRNA. Cyclosporine orsplenectomy alone could not prolong theSurvival time, however, their combination couldcompletely repress the rejection of liver grafts.The survival time of animals were significantlyprolonged (37.1 days ). The architecture ofhepatic lobules was preserved. There was slightcellular infiltration in the portal tracts and noexpression of perform and granzyme B genesmRNA could be seen in three weeks aftertransplantation.CONCLUSION Perform and granzyme B genesare valuable in judging the effect ofimmunosuppression in liver transplantation. | Zhang SG Wu MC Tan JW Chen H Yang JM Qian QJ | 1999 | World Journal of Gastroenterology1999，5，3： | 15 |
| 4 | 急性缺血性脑卒中后低脉压与不良预后相关显示文摘众所周知,脉压与心脏功能、动脉僵硬度、体液状态和血管事件有关。该研究旨在探讨基于中国台湾地区脑卒中登记资料的急性缺血性脑卒中(acute ischemic stroke,AIS)患者入院时脉压的预后作用。 | 刘莉 叶鹏 Tang SC Yin JH Liu CH Sun MH Lee JT Sun Y Hsu CS Sun MC Lin CH Chen CH Lien LM Muo CH Jeng JS Hsu CY | 2017 | 中华高血压杂志2017，25，7： | 5 |
| 5 | 2,3-Diaryl-3H-imidazo[4,5-b]pyridine derivatives as potential anticancer and anti-inflammatory agents显示文摘In this study we examined the suitability of the 3H-imidazo[4,5-b]pyridine ring system in developing novel anticancer and anti-inflammatory agents incorporating a diaryl pharmacophore. Eight 2,3-diaryl-3H-imidazo[4,5-b]pyridine derivatives retrieved from our in-house database were evaluated for their cytotoxic activity against nine cancer cell lines. The results indicated that the compounds showed moderate cytotoxic activity against MCF-7, MDA-MB-468, K562 and SaOS2 cells, with K562 being the most sensitive among the four cancer cell lines. The eight 2,3-diaryl-3H-imidazo[4,5-b]pyridine derivatives were also evaluated for their COX-1 and COX-2 inhibitory activity in vitro. The results showed that compound 3f exhibited 2-fold selectivity with IC_(50) values of 9.2 and 21.8 mmol/L against COX-2 and COX-1, respectively. Molecular docking studies on the most active compound 3f revealed a binding mode similar to that of celecoxib in the active site of the COX-2 enzyme. | Erin Marie Kirwen Tarun Batra Chandrabose Karthikeyan Girdhar Singh Deora Vandana Rathore Chaitanya Mulakayala Naveen Mulakayala Amy Catherine Nusbaum Joel Chen Haneen Amawi Kyle Mc Intosh Sahabjada Neelam Shivnath Deepak Chowarsia Nisha Sharma Md Arshad Piyush Trivedi Amit KTiwari | 2017 | Acta Pharmaceutica Sinica B2017，7，1： | 3 |
| 6 | Medial sural artery perforator flap for intraoral reconstruction following cancer ablation 显示文摘 | Chen SL Yu CC Chang MC | 2008 | Ann Plastic Surgery2008，61，3： | 1 |
| 7 | Optimal surgical strategy for potentially curable serosa-involved gastric carcinoma with intraperitontoneal free cancer cells 显示文摘 | Wu CC Chen JT Chang MC | 1997 | J Am Coll Surg1997，184，6： | 1 |
| 8 | Effect of adsorbents coated with titanium dioxide on the photocatalytic degradation of propoxur 显示文摘 | Lu MC Chen J N Chang K T etal | 1999 | Chemosphere1999，38，： | 1 |
| 9 | International Nanotechnology Development in 2003 : Country, Institution, and Technology Field Analysis Based on USPTO Patent Data- base显示文摘 | Huang Z Chen H Chen Z Roco MC | 2004 | Journal of Nanoparticle Research2004，6，4： | 1 |
| 10 | Self-heated fiber Bragg grating sensors显示文摘 | Chen K P Mc Millen B Buric M | 2005 | Applied Physics Letters2005，86，14： | 1 |
| 11 | Physicochemical basis for binding and voltage-dependent block of hERG channels by structurally diverse drugs显示文摘 | Sanguinetti MC Chen J Fernandez D | 2005 | Novartis Found Symp2005，266，： | 1 |
| 12 | Chronic obstructive pulmonary disease is associated with lung cancer mortality in a prospective study of never smokers 显示文摘 | TURNER MC CHEN Y KREWSKI D | 2007 | Am J Respir Crit Care Med2007，176，3： | 1 |
| 13 | A plase Ⅱ trail of vinorelbine plus gemcitabine in previously untreated inoperable(stage Ⅲ b/Ⅳ)non-small-cell lung cancer patients aged 80 or older显示文摘 | Chen YM Peng RP Chen MC | 2003 | Lung Cancer2003，40，2： | 1 |
| 14 | Combination transcatheter hepatic arterial chemoembolization with thymosin alpha 1 on recurrence prevention of hepatocellular carcinoma显示文摘 | Cheng SQ Wu MC Chen H | 2004 | Hepatogastroenterology2004，51，59： | 1 |
| 15 | Worldwide Nanotech- nology Development: A Comparative Study of USPTO, EPO, and JPO Patents ( 1976 - 2004) 显示文摘 | Li X Lin Y Chen H Roco MC | 2007 | Journal of Nanoparticle Research2007，9，6： | 1 |
| 16 | Mortality risk factors in patients with Acinetobacter baumannii ventilator-associated pneumonia显示文摘 | Chang HC Chen YC Lin MC | 2011 | J Formos Med Assoc2011，110，9： | 1 |
| 17 | Stabilization of blood meth- ylmalonic acid level in methylmalonie acidemia after liver transplantation显示文摘 | Chen PW Hwu WL Ho MC | 2010 | Pediatr Transplant2010，14，3： | 1 |
| 18 | lnvolvement of cyclooxyge- nase 2 and prostaglandin E(2) in the effects of insulin on gastric emp- tying in male rats显示文摘 | Huang WJ Hung CR Chen MC | 2009 | Acta physiologica Polonica2009，60，3： | 1 |
| 19 | Structural Biological Materials:Critical Mechanics-Materials Connections显示文摘 | Meyers M A Mc Kittrick J Chen P Y | 2013 | Science2013，339，6121： | 1 |
| 20 | Clinical features and outcome of coronary artery aneurysm in patients with acute myocardial infarction undergoing a primary percutaneous coronary intervention显示文摘 | Yip HK Chen MC Wu CJ | 2002 | Cardiology2002，98，3： | 1 |
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