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| 1 | CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients显示文摘AIM To characterize peripheral blood natural killer(NK) cells phenotypes by flow cytometry as potential biomarker of liver fibrosis in human immunodeficiency virus(HIV)/hepatitis C virus(HCV) coinfected patients.METHODS Peripheral mononuclear cells from 24 HIV/HCV(HBVnegative) coinfected and 5 HIV/HCV/HBV seronegative individuals were evaluated. HIV/HCV coinfected patients were divided in to groups: G1, patients with METAVIR F0-F2 and G2, patients with METAVIR F3-F4. NK surface cell staining was performed with: AntiCD3(APC/Cy7), anti-CD56(PE/Cy5), anti-CD57(APC), anti-CD25(PE), anti-CD69(FITC), anti-NKp30(PE), antiNKp46(PE/Cy7), anti-NKG2D(APC), anti-DNAM(FITC); anti-CD62L(PE/Cy7), anti-CCR7(PE), anti-TRAIL(PE), anti-Fas L(PE), anti CD94(FITC). Flow cytometry data acquisition was performed on BD FACSCanto, analyzed using Flow Jo software. Frequency of fluorescence was analyzed for all single markers. Clinical records were reviewed, and epidemiological and clinical data were obtained.RESULTS Samples from 11 patients were included in G1 and from 13 in G2. All patients were on ARV, with undetectable HIV viral load. Liver fibrosis was evaluated by transient elastography in 90% of the patients and with biopsy in 10% of the patients. Mean HCV viral load was(6.18 ± 0.7 log10). Even though, no major significant differences were observed between G1 and G2 regarding NK surface markers, it was found that patients with higher liver fibrosis presented statistically lower percentage of NK cells than individual with low to mild fibrosis and healthy controls(G2: 5.4% ± 2.3%, G1: 12.6% ± 8.2%, P = 0.002 and healthy controls 12.2% ± 2.7%, P = 0.008). It was also found that individuals with higher liver fibrosis presented lower CD4 LT count than those from G1(G2: 521 ± 312 cells/μL, G1: 770 ± 205 cells/μL; P = 0.035).CONCLUSION Higher levels of liver fibrosis were associated with lower percentage of NK cells and LTCD4+ count; and they may serve as noninvasive biomarkers of liver damage. | Natalia Laufer Diego Ojeda María Laura Polo Ana Martinez Héctor Pérez Gabriela Turk Pedro Cahn Norberto Walter Zwirner Jorge Quarleri | 2017 | World Journal of Hepatology2017，9，25： | 2 |
| 2 | Human plasmacytoid dendritic cells express receptors for anaphylatoxins c3a and c5a and are chemoattracted to c3a and c5a显示文摘 | K(o)ther B Zwirner J | 2006 | J Invest Dermatol2006，126，11： | 1 |
| 3 | Participation in multi-criteria decision support for the resolution of a waterallocation problem in the Spree River basin显示文摘 | FRANK MESSNER OLIVER ZWIRNER MATTHIAS KARKUSCHKE | 2006 | Land Use Policy2006，23，1： | 1 |
| 4 | Cytokine regulation of natural killer cell effector functions显示文摘 | Zwirner NW Domaica CI | 2010 | Biofactors2010，36，4： | 1 |
| 5 | Expression of the anaphylatoxin C5α receptor in non-myeloid cells显示文摘 | Zwirner J Fayyazi A Gotze O | 1999 | Mol Immunol1999，36，1314： | 1 |
| 6 | Functional assessment of patients with spasmodic dysphonia显示文摘 | WOODSON G E ZWIRNER P MURRY T | 1992 | Journal of Voice1992，6，： | 1 |
| 7 | Stability of insulin-like growth factor (IGF)-1 and IGF binding protein (IGFBP-3) measured by the IMMULITE automated chemiluminescence assay system in different blood specimens显示文摘 | Elmlinger MW Zwirner M Kühnel W | 2005 | Clin Lab2005，51，： | 1 |
| 8 | Cytokine-driven regulation of NK cell functions in tumor immunity:role of the MICA-NKG2D system显示文摘 | Zwirner N W Fuertes M B Girart M V | 2007 | Cytokine Growth Factor Rev2007，18，： | 1 |
| 9 | Targeted inhibition of galectin-1 gene expression in tumor cells results in heightened T cell-mediated rejection:A potential mechanism of tumor-immune privilege显示文摘 | Natalia Rubinstein Mariano Alvarez Norberto W Zwirner | 2004 | Cancer Cell2004，5，： | 1 |
| 10 | Targeted inhibition of galectin-1 gene expression in tumor cells results in heightened T cell-mediated rejection:A potential mechanism of tumor-immune privilege显示文摘 | Rubinstein N Alvarez M Zwirner NW | 2004 | Cancer Cell2004，5，3： | 1 |
| 11 | Expression of the anaphylatoxin C5a receptor in non-myeloid cells显示文摘 | Zwirner J Fayyazi A Gotze O | 1999 | Mol Immunol1999，36，4： | 1 |
| 12 | Differential surface expression of MIC by endothelial ceils,fibmblasts,keratinecytes,and monocytes显示文摘 | Zwirner NW Dole K Stasmy P | 1999 | Hum Immunol1999，60，4： | 1 |
| 13 | Identification of MICAas a new polymorphic alloantigen recognized by antibodies in seraof organ transplant recipients显示文摘 | Zwirner NW Marcos CY Mirbaha F | 2000 | Hum Immunol2000，61，9： | 1 |
| 14 | Participation in multi-criteria decision support for the resolution of a water allocation problem in the Spree River basin显示文摘 | Frank Messner Oliver Zwirner Matthias Karkuschke | 2006 | Land Use Policy2006，23，1： | 1 |
| 15 | Targeted in- hibition of galectin-I gene expression in tumor cells re- suits in heightened T cell-mediated rejeetion A potential mechanism of tumor-immune privilege显示文摘 | Rubinstein N Alvarez M Zwirner NW | 2004 | Cancer Cell2004，5，3： | 1 |
| 16 | Dendritic cells:limited potential in immunotherapy显示文摘 | Soruri A Zwirner J | 2005 | The International Journal of Biochemistry & Cell Biology2005，37，： | 1 |
| 17 | Activated complement C3: a potentially novel predictor of progressive IgA nephropathy显示文摘 | Zwirner J Burg M Schulze M | 1997 | Kidney Int1997，51，： | 1 |
| 18 | Complement activation in human lymphoid germinal centres显示文摘 | Zwirner J Felber E Schmidt P | 1989 | Immunology1989，66，2： | 1 |
| 19 | Targeted inhibition of Galectin-1 gene expression in tumor cells results in heightened T cell-mediated rejection; a potential mechanism of tumor-immune privilege显示文摘 | Rubinstein N Alvarez M Zwirner NW | 2004 | Cancer Cell2004，5，3： | 1 |
| 20 | Examination of telomere length in muscle tissue casts doubt on replicative aging as cause of progression in Duchenne muscular dystrophy显示文摘 | Oexle K Zwirner A Freudenberg AA | 1997 | Pediatr Res1997，42，： | 1 |
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