| 1 | The antiapoptotic effect of insulin against anoxia/reoxygenation injury in cultured cardiomyocyte of neonatal rat显示文摘Objective: To study protective effect of insulin against cardiomyocyte apoptosis in anoxia/reoxygenation (A/R) injury of neonatal rat. Methods:The model of A/R injury was finished through receiving anoxia for 2 h and reoxygenation for 4 h in cultured cardiomyocytes of neonatal rat. The cardiomyocytes were divided randomly into 3 groups: control group (CON), anoxia/reoxygenation group (A/R) and insulin-treated group (INS). At the end of reoxygenation of 4 hours, activities of lactate dehydrogenase (LDH), contents of malondialdehyde (MDA) were assessed through spectrophotometric procedures, myocyte apoptosis were detected through TUNEL and DNA Ladder. Results: MDA, LDH, and Apoptosis Index were significantly decreased in INS group compared with A/R group (P<0.01). Conclusion: Insulin has a protective effect against A/R injury in cultured cardiomyocyte of neonatal rat; the protective mechanism may contribute to antiapoptosis of insulin. | Xiang Gu Chunzhi Shi Yibai Feng Ming Li Zuolin Fu Xinping Zhang | 2005 | Journal of Nanjing Medical University2005，19，5： | 1 |
| 2 | Release of myocardial noradrenaline during acute hibernation in the isolated rat heart显示文摘Objective: To explore the release of myocardial noradrenaline during acute hibernation. Methods: The hearts were gained from rats and set up as modified Langendorf preparations beating isometrically. They were perfused with modified Krebs-Henseleit buffer under controlled pressure. Mechanical measurements and coronary effluent were recorded simultaneously at 30 min intervals for 150 min. Lactate dehydrogenase in coronary effluent was assayed at the beginning, 60 min and 120 min low-flow ischemia. Noradrenaline in coronary effluent was determined at the beginning of low-flow and 120 min of low-flow ischemia and also in control, during hibernation and after 30 min reperfusion during stimulation, myocardial noradrenaline response on tyramine was investigated in absence or presence of desipramine after 30 min reperfusion. Results: In the control, there was nosignificant chant in noradrenaline overflow during 120 min perfusion; In the acute myocardial hibernation group, there was also nosignificant difference in noradrenaline overflow between the beginning and 120 min low-flow ischemia. The electrical field stimulation-induced overflow of noradrenaline during hibernation myocardium was significantly less than preischemia or after reperfusion, but there was nosignificant difference between preischemia and reperfusion group. Tyramine induced significant noradrenaline release in absence of desipramine after 30 min reperfusion, but this increase in noradrenaline release had nosignificant in the presence of desipramine. These studies indicated that there was not significant spontaneous noadrenaline overflow during acute myocardial hibernation in isolated rat hearts, the stimulation-induced noradrenaline overflow decreased during hibernation and restored to the level of preischemia after reperfusion, myocardial noradrenaline response to tyramine remained after 30 min reperfusion. Conclusion: Myocardial noradrenaline overflow may not contribute to the development of acute myocardial hibernation and the function of sympathetic nerve may also maintain in hibernation as myocardium does during acute myocardial hibernation, reperfusion of myocardium may contribute to restoring the function of sympathetic nerve. | Zuolin Fu Yibai Feng Hongxia Xu Jiang Xie Xinping Zhang Chunzhi Shi Xiang Gu Ming Li | 2005 | Journal of Nanjing Medical University2005，19，5： | 1 |
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