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| 1 | Curative effect of heat-sensitive moxibustion on chronic persistent asthma: a multicenter randomized controlled trial显示文摘OBJECTIVE:To compare the curative effects of heat-sensitive moxibustion with conventional drugs on chronic persistent asthma and seek a valuable therapy to replaceWestern Medicine.METHODS:The participants in this multi-center,randomized,and controlled study were randomly divided into two groups:group A(n=144),treated with heat-sensitive moxibustion(50 sessions)and group B(n=144),treated with Seretide(salmeterol50μg/fluticasone 250μg,twice a day).The scores of asthma control test(ACT),forced expiratory volume in 1 second(FEV1),peak expiratory flow(PEF),and attack frequency were measured after 15,30,60,and 90 days of treatment.Patients followed up3 and 6 months after treatment.RESULTS:There was a significant difference(P=0.0002)in the ACT score and lung function between the two groups after 3 months of treatment and(P=0.000 03)during the follow-up visits.In addition,heat-sensitive moxibustion reduced attack frequency in the period from inclusion to the6-month follow-up visit.CONCLUSION:This study shows that heat-sensitive moxibustion may have a comparable curative effect toSeretide(salmeterol/fluticasone)on asthma. | Rixin Chen Mingren Chen Jun Xiong Zhenhai Chi B0 Zhang Ning Tian Zhenhua Xu Tangfa Zhang Wanyao Li Wei Zhang Xiaofeng Rong Zhen Wang Gang Sun Baohe Ge Guoxiong Yu Nanchang Song | 2013 | Journal of Traditional Chinese Medicine2013，33，5： | 20 |
| 2 | The Predictive Value of Baseline HBs Ag Level and Early Response for HBs Ag Loss in Patients with HBe Ag-positive Chronic Hepatitis B during Pegylated Interferon Alpha-2a Treatment显示文摘Objective To explore the predictive value of baseline HBsAg level and early response for HBsAg loss in patients with HBeAg-positive chronic hepatitis B during pegylated interferon alpha-2a treatment. Methods A total of 121 patients with HBeAg-positive chronic hepatitis B who achieved HBsAg loss were enrolled; all patients were treated with PEG-IFNα-2a 180 μg/week. Serum HBV DNA and serological indicators(HBsAg, anti-HBs, HBeAg, and anti-HBe) were determined before and every 3 months during treatment. Results The median treatment time for HBsAg loss was 84 weeks(7-273 weeks), and 74.38%(90 cases) of the patients needed extended treatment(> 48 weeks). The correlation between baseline HBsAg levels and the treatment time of HBsAg loss was significant(B = 14.465, t = 2.342, P = 0.021). Baseline HBsAg levels together with the decline range of HBsAg at 24 weeks significantly correlated with the treatment time of HBsAg loss(B = 29.862, t = 4.890, P = 0.000 and B = 27.993, t = 27.993, P = 0.005). Conclusion Baseline HBsAg levels and extended therapy are critical steps toward HBsAg loss. Baseline HBsAg levels together with early response determined the treatment time of HBsAg loss in patients with HBeAg-positive chronic hepatitis B during pegylated interferon alpha-2a treatment. | LI Ming Hui ZHANG Lu QU Xiao Jing LU Yao SHEN Ge LI Zhen Zhen WU Shu Ling LIU Ru Yu CHANG Min HU Lei Ping HUA Wen Hao SONG Shu Jing WAN Gang XIE Yao | 2017 | Biomedical and Environmental Sciences2017，30，3： | 14 |
| 3 | Design of the Full-disk Magneto Graph (FMG) onboard the ASO-S显示文摘The Full-disk Magneto Graph(FMG),a payload onboard the Advanced Space Solar Observatory(ASO-S),will measure the vector magnetic field in the photosphere.The instrument consists of a frontwindow filter,a telescope,an LCVR polarimeter,an image-stabilization system,a seven-stage tunable Lyot filter,a CMOS camera with 4096×4096 pixels and a pair of calibration/focus wheels.In this paper,we describe the design of the FMG instrument and provide a summary of test observations carried out with the FMG prototype. | Yuan-Yong Deng Hai-Ying Zhang Jian-Feng Yang Fu Li Jia-Ben Lin Jun-Feng Hou Zhen Wu Qian Song Wei Duan Xian-Yong Bai Dong-Guang Wang Juan Lv Wei Ge Jia-Ning Wang Zhao-Ying Zheng Chen-Jie Wang Nan-Ge Wang Hou-Kun Ni Yi-Zhong Zeng Yang Zhang Xiao Yang Ying-Zi Sun Zhi-Yong Zhang Xiao-Fan Wang | 2019 | Research in Astronomy and Astrophysics2019，19，11： | 14 |
| 4 | Comprehensive analysis of NAC transcription factors in diploid Gossypium: sequence conservation and expression analysis uncover their roles during fiber development显示文摘Determining how function evolves following gene duplication is necessary for understanding gene expansion.Transcription factors(TFs)are a class of proteins that regulate gene expression by binding to specific cis-acting elements in the promoters of target genes,subsequently activating or repressing their transcription.In the present study,we systematically examined the functional diversification of the NAC transcription factor(NAC-TFs)family by analyzing their chromosomal location,structure,phylogeny,and expression pattern in Gossypium raimondii(Gr)and G.arboreum(Ga).The 145 and 141 NAC genes identified in the Gr and Ga genomes,respectively,were annotated and divided into 18 subfamilies,which showed distinct divergence in gene structure and expression patterns during fiber development.In addition,when the functional parameters were examined,clear divergence was observed within tandem clusters,which suggested that subfunctionalization had occurred among duplicate genes.The expression patterns of homologous gene pairs also changed,suggestive of the diversification of gene function during the evolution of diploid cotton.These findings provide insights into the mechanisms underlying the functional differentiation of duplicated NAC-TFs genes in two diploid cotton species. | Haihong Shang Zhongna Wang Changsong Zou Zhen Zhang Weijie Li Junwen Li Yuzhen Shi Wankui Gong Tingting Chen Aiying Liu Juwu Gong Qun Ge Youlu Yuan | 2016 | Science China(Life Sciences)2016，59，2： | 12 |
| 5 | Association Between Diurnal Temperature Range and Respiratory Tract Infections显示文摘Objective This study aimed to assess the association between emergency-room visits for respiratory tract infection (RTI) with diurnal temperature range (DTR), a weather parameter closely associated with urbanization and global climate change. Methods We conducted a semiparametric time-series analysis to estimate the percentage increase in emergency-room visits for RTI associated with changes in DTR after adjustment for daily weather conditions (temperature and relative humidity) and outdoor air pollution. Results DTR was significantly associated with daily emergency-room visits for RTI. An increase of 1 °C in the current-day (L0) and in the 2-day moving average (L01) DTR corresponded to a 0.94% [95% confidence interval (CI), 0.34%-1.55%] and 2.08% (95% CI, 1.24%-2.93%) increase in emergency-room visits for RTI, respectively. Conclusion DTR was associated with increased risk of RTI. More studies are needed to understand the impact of DTR on respiratory health. | GE Wen Zhen XU Feng ZHAO Zhuo Hui ZHAO Jin Zhuo KAN Hai Dong | 2013 | Biomedical and Environmental Sciences2013，26，3： | 10 |
| 6 | In vitro assessment of the glucose-lowering effects of berberrubine-9-O-β-D-glucuronide, an active metabolite of berberrubine显示文摘Berberrubine (BRB ) 是在 vivo 比 BBR 显示出更强壮的葡萄糖阴沉的效果的 berberine (BBR ) 的主要代谢物。在另一方面, BRB 快速并且广泛地被使产生代射变化进 berberrubine-9-O -- 在在口头的管理以后的老鼠的 D-glucuronide (BRBG ) 。在这研究,我们在老鼠比较了 BRB 和 BRBG 的 pharmacokinetic 性质,并且探索了位于他们的葡萄糖阴沉的活动下面的机制。有导致 HFD 的多糖症的 C57BL/6 老鼠被管理 BRB (50 mg 吗?? | Na YANG Run-bin SUN Xing-long CHEN Le ZHEN Chun GE Yu-qing ZHAO Jun HE Jian-liang GENG Jia-hua GUO Xiao-yi YU Fei FEI Si-qi FENG Xuan-xuan ZHU Hong-bo WANG Feng-hua FU Ji-ye AA Guang-ji WANG | 2017 | Acta Pharmacologica Sinica2017，38，3： | 9 |
| 7 | Geometrical feature analysis and disaster assessment of the Xinmo landslide based on remote sensing data显示文摘At 5:39 am on June 24, 2017, a landslide occurred in the village of Xinmo in Maoxian County, Aba Tibet and Qiang Autonomous Prefecture(Sichuan Province, Southwest China). On June 25, aerial images were acquired from an unmanned aerial vehicle(UAV), and a digital elevation model(DEM) was processed. Landslide geometrical features were then analyzed. These are the front and rear edge elevation, accumulation area and horizontal sliding distance. Then, the volume and the spatial distribution of the thickness of the deposit were calculated from the difference between the DEM available before the landslide, and the UAV-derived DEM collected after the landslide. Also, the disaster was assessed using high-resolution satellite images acquired before the landslide. These include Quick Bird, Pleiades-1 and GF-2 images with spatial resolutions of 0.65 m, 0.70 m, and 0.80 m, respectively, and the aerial images acquired from the UAV after the landslide with a spatial resolution of 0.1 m. According to the analysis, the area of the landslide was 1.62 km2, and the volume of the landslide was 7.70 ± 1.46 million m3. The average thickness of the landslide accumulation was approximately 8 m. The landslide destroyed a total of 103 buildings. The area of destroyed farmlands was 2.53 ha, and the orchard area was reduced by 28.67 ha. A 2-km section of Songpinggou River was blocked and a 2.1-km section of township road No. 104 was buried. Constrained by the terrain conditions, densely populated and more economically developed areas in the upper reaches of the Minjiang River basin are mainly located in the bottom of the valleys. This is a dangerous area regarding landslide, debris flow and flash flood events Therefore, in mountainous, high-risk disaster areas, it is important to carefully select residential sites to avoid a large number of casualties. | FAN Jian-rong ZHANG Xi-yu SU Feng-huan GE Yong-gang Paolo TAROLLI YANG Zheng-yin ZENG Chao ZENG Zhen | 2017 | Journal of Mountain Science2017，14，9： | 8 |
| 8 | C/EBPα regulates SIRT1 expression during adipogenesis显示文摘SIRT1 在 adipogenesis 起一个重要作用,但是 SIRT1 怎么在 adipogenesis 被调整,大部分是未知的。在这研究,我们证明 SIRT1 蛋白质和 mRNA 层次与 CCAAT/enhancer-binding 蛋白质伪一起被增加(C/EBP 伪) 在 adipocyte 区别期间。C/EBP 伪,然而并非 C/EBP 伪 p 30,在 HeLa 房间和 3T3-L1 的激活的 SIRT1 倡导者 preadipocytes。而且, C/EBP 伪 upregulated SIRT1 mRNA 和蛋白质在 Soas2 房间以一种 p53 独立的方式在 HeLa 铺平房间和增加的 SIRT1 表示。在 preadipocytes, C/EBP 伪 upregulated SIRT1 蛋白质水平的宫外的表示并且导致的 C/EBP 伪击倒 SIRT1 的减少蛋白质水平。而且由倡导者删除分析,胶化移动试金和染色质 immunoprecipitation,我们发现了那 C/EBP,伪在一个一致 C/EBP 伪绑定地点跳了到 SIRT1 倡导者。这些数据证明那 C/EBP 伪在 adipogenesis 期间调整 SIRT1 表示由对 SIRT1 倡导者直接有约束力。 | Qihuang Jin Fang Zhang Tingting Yan Zhen Liu Chunxi Wang Xinjian Ge Qiwei Zhai | 2010 | Cell Research2010，20，4： | 8 |
| 9 | Discovering differential protein expression caused by CagA-induced ERK pathway activation in AGS cells using the SELDI-ProteinChip platform显示文摘AIM: To identify the protein expression differences related to the CagA-induced ERK pathway activation in AGS cells. METHODS: Human AGS cells transfected with cagA and blank vector were treated with specific mitogen-activated protein kinase kinase (MEK) inhibitor. Total cell proteins were combined by strong anion exchange (SAX2) and weak cation exchange (CM10) ProteinChip arrays and analyzed using surface-enhanced laser desorption/ ionization time-of-flight mass spectrometry (SELDI-TOF-MS) proteomics technology. Protein expression profi les were compared with those of inhibitor-untreated cagA transfectants. SwissProt/TrEMBL database searching for differentially expressed proteins was carried out using the TagIdent tool with the pI and mass information. RESULTS: When a total of 16 proteins that showed expression differences in inhibitor-untreated cagA transfectants were compared with vector transfectants,three proteins with m/z 4229,8162 and 9084 were found to have no expression differences after treatment with MEK inhibitor,while the other 13 maintained the same expression differences after inhibitor treatment. Seven pieces of meaningful matching information for the three proteins were obtained from database searching. CONCLUSION: Biomarkers with m/z 4229,8162 and 9084 are ERK1/2 phosphorylation dependent,and therefore are the downstream molecules of ERK1/2 in the ERK/MAPK signaling pathway. The three biomarkers may be important cancer-associated proteins according to SwissProt/TrEMBL database information. | Zhen Ge Yong-Liang Zhu Xian Zhong Jie-Kai Yu Shu Zheng | 2008 | World Journal of Gastroenterology2008，14，4： | 8 |
| 10 | Distribution and characteristic of PAHs in snow of Fildes Peninsula显示文摘Polycyclic aromatic hydrocarbons (PAHs) investigation in different matrices has been reported largely,whereas reports on snow samples were limited.Snow,as the main matrix in the polar region,has an important study meaning.PAHs in snow samples were analyzed to investigate the distribution and contamination status of them in the Antarctic,as well as to provide some references for global migration of PAHs.Snow samples collected in Fildes Peninsula were enriched and separated by solid-phase membrane disks and eluted by methylene dichloride,then quantified by gas chromatography/mass spectrometry.All types of PAHs were detected except for Benzo(a)pyrene.Principal component analysis method was applied to characterize them.Three factors (Naphthalene,Fluorene and Phenanthrene) accounted for 60.57%,21.61% and 9.80%,respectively.The results showed that the major PAHs sources maybe the atmospheric transportation,and the combustion of fuel in Fildes Peninsula.The comparison of concentration and types of PAHs between accumulated snow and fresh snow showed that the main compound concentrations in accumulated snow samples were higher than those in fresh ones.The risk assessment indicated that the amount of PAHs in the snow samples would not lead to ecological risk. | Guangshui Na Chunyang Liu Zhen Wang Linke Ge Xindong Ma Ziwei Yao | 2011 | Journal of Environmental Sciences2011，23，9： | 8 |
| 11 | The critical roles of m6A modification in metabolic abnormality and cardiovascular diseases显示文摘N6-methyladenosine(m6A)RNA methylation is an emerging area of epigenetics,which is a reversible and dynamic modification mediating by‘writers’(methylase,adding methyl groups,METTL3,METTL14,and WTAP),‘erasers’(demethylase,deleting methyl groups,FTO and ALKBH5),and‘readers’(YTHDF1-3,YTHDC1 and YTHDC2).Recent studies in human,animal models and cell levels have disclosed a critical role of m6A modification in regulating the homeostasis of metabolic processes and cardiovascular function.Evidence from these studies identify m6A as a candidate of biomarker and therapeutic target for metabolic abnormality and cardiovascular diseases(CVD).Comprehensive understanding of the complexity of m6A regulation in metabolic diseases and CVD will be helpful for us to understand the pathogenesis of CVD.In this review,we discuss the regulatory role of m6A in metabolic abnormality and CVD.We will emphasize the clinical relevance of m6A dysregulation in CVD. | Beijian Zhang Hao Jiang Zhen Dong Aijun Sun Junbo Ge | 2021 | Genes & Diseases2021，8，6： | 8 |
| 12 | Synthesis of a Novel Core-shell Type Acrylic-polyurethane Hybrid Emulsion Containing Siloxane and Fluorine as well as Water and the Oil Resistances of Cured Film显示文摘Siliconated polyurethane (Si-PU) was synthesized using isophorone diisocyanate (IPDI), hydroxybutyl-terminated polydimethylsiloxane (PDMS), polytetramethylene ether glycol (PTMG), polypropylene glycol (PPG), 1,6-hexanediol (HDO), dimethylol propionic acid (DMPA) and triethylamine (TEA). Based on butyl acrylate (BA), 2, 2, 2-trifluoroethylmethacrylate (TFEMA) and Si-PU as a seed emulsion, a novel core-shell type acrylic-polyurethane hybrid emulsion, containing siloxane and fluorine (F-Si-PU), was prepared by seeded emulsion polymerization. The contents of siloxane and fluorine were determined according to the feed ratio. Fourier transform infrared spectroscopy (FTIR) was used to identify the chain structures of Si-PU and F-Si-PU. Investigation of transmission electron microscopy (TEM) confirmed the core-shell structure of F-Si-PU emulsion. Measurement results of water contact angle and the swelling ratio in water and n-octane for cured film showed that the water and the oil resistances for F-Si-PU had been significantly improved at a suitable content of fluorine and siloxane. | Jing CHAO Xing Yuan ZHANG Jia Bing DAI Zhen GE Lin Lin FENG | 2006 | Chinese Chemical Letters2006，17，8： | 7 |
| 13 | Characteristics of transmitting channel wave in a coal seam显示文摘An embedded underground coal seam carries channel waves of low seismic velocity along a stratigraphic rock-coal-rock sequence.In a homogeneous and isotropic seam, seismic waves propagate as trapped waves within the seam, which leads to propagation of channel waves.We describe how to set up a field test for transmission in order to acquire channel waves in a coal seam.Because channel wave signals are non-stationary in their frequencies and amplitudes, a necessary velocity spectrum and wavelet transformation analysis are applied to interpret the characteristics of channel waves.The advantage of using a wavelet transformation is that different resolutions can be obtained at different times and different frequencies.According to analysis of the seismic signals acquired in the S7 sensor hole, it was clearly shown that the characteristics of channel waves are lower frequencies and attenuation which can guide an effective wave for detecting voids, boundaries and faults in coal seams with strong roofs and floors. | YANG Zhen GE Mao-chen WANG Shu-gang | 2009 | Mining Science and Technology2009，19，3： | 7 |
| 14 | An experimental study on astrocytes promoting production of neural stem cells derived from mouse embryonic stem cells显示文摘Background The production of neural stem cells (NSCs) derived from embryonic stem (ES) cells was usually very low according to previous studies, which was a major obstacle for meeting the needs of clinical application. This study aimed at investigating whether astrocytes could promote production of NSCs derived from ES cells in vitro.Methods Mouse ES cells line-D3 was used to differentiate into NSCs with astrocytes as inducing stromal cells by means of three-stage differentiation procedure. Another group without astrocytes served as control. The totipotency of ES cells was identified by observation of cells’ morphology and formation of teratoma in severe combined immunodeficiency disease (SCID) mice. The quantity and purity of NSCs derived from ES cells were analyzed using clonogenic assay, immunohistochemical staining and flow cytometry assay. The plasticity of NSCs was detected by differentiating test. Octamer-binding transcription factor 4 (Oct-4) and nestin, the specific marker genes of ES cells and NSCs respectively, were detected continuously using reverse transcription-polymerase chain reaction (RT-PCR) method to monitor the process of cell differentiation.Results The ES cells of D3 line could maintain the ability of differentiating into cellular derivations of all three primary germ layers after continuous passage culture. At the end of two-stage of inducing process, 23.2±3.5 neurospheres per plate formed in astrocyte-induced group and only 0.8±0.3 per plate in the control group (clonogenic assay, P<0.01), and the ratio of nestin positive cells was (50.2±2.8)% in astrocyte-induced group and only (1.4±0.5)% in the control group (flow cytometry, P<0.01). With the induction undergoing, the expression of Oct-4 gradually decreased and then disappeared, while the expression of nestin was increased step by step, and the ratio of nestin positive cells was up to 91.4% by the three-stage differentiation. The nestin positive cells could be further induced into neurons, astrocytes, and oligodendrocytes in differentiating medium supplemented with fetal calf serum. The results of differentiating test showed that the ratio of NF-200 and NSE positive cells was (42.7±2.6)% in astrocyte-induced group and only (11.2±1.8)% in the control group (P<0.01).Conclusions Astrocytes can not only increase the production of NSCs derived from ES cells but also promote the differentiation of NSCs toward neuronal lineage. | ZHOU Yu-feng FANG Feng FU Jin-rong DONG Yong-sui YE Du-yun SHU Sai-nan ZHEN Hong LI Ge | 2005 | Chinese Medical Journal2005，，23： | 7 |
| 15 | Massive cranium from Harbin in northeastern China establishes a new Middle Pleistocene human lineage显示文摘It has recently become clear that several human lineages coexisted with Homo sapiens during the late Middle and Late Pleistocene.Here,we report an archaic human fossil that throws new light on debates concerning the diversification of the Homo genus and the origin of H.sapiens.The fossil was recovered in Harbin city in northeastern China,with a minimum uranium-series age of 146 ka.This cranium is one of the best preserved Middle Pleistocene human fossils.Its massive size,with a large cranial capacity(1,420 mL)falling in the range of modern humans,is combined with a mosaic of primitive and derived characters.It differs from all the other named Homo species by presenting a combination of features,such as long and low cranial vault,a wide and low face,large and almost square orbits,gently curved but massively developed supraorbital torus,flat and low cheekbones with a shallow canine fossa,and a shallow palate with thick alveolar bone supporting very large molars.The excellent preservation of the Harbin cranium advances our understanding of several less-complete late Middle Pleistocene fossils from China,which have been interpreted as local evolutionary intermediates between the earlier species Homo erectus and later H.sapiens.Phylogenetic analyses based on parsimony criteria and Bayesian tip-dating suggest that the Harbin cranium and some other Middle Pleistocene human fossils from China,such as those from Dali and Xiahe,form a third East Asian lineage,which is a part of the sister group of the H.sapiens lineage.Our analyses of such morphologically distinctive archaic human lineages from Asia,Europe,and Africa suggest that the diversification of the Homo genus may have had a much deeper timescale than previously presumed.Sympatric isolation of small populations combined with stochastic long-distance dispersals is the best fitting biogeographical model for interpreting the evolution of the Homo genus. | Xijun Ni Qiang Ji Wensheng Wu Qingfeng Shao Yannan Ji Chi Zhang Lei Liang Junyi Ge Zhen Guo Jinhua Li Qiang Li Rainer Grun Chris Stringer | 2021 | The Innovation2021，2，3： | 6 |
| 16 | m6A demethylase FTO attenuates cardiac dysfunction by regulating glucose uptake and glycolysis in mice with pressure overload-induced heart failure显示文摘Dear Editor,N6-methyladenosine(m6A)is the most common posttranscriptional mammalian mRNA modification involved in multiple biological processes and diseases.1 However,its role in cardiac energy metabolism changes during heart failure(HF)remains elusive.Here we explored the role of m6A demethylase fat mass and obesity-associated protein(FTO)in glucose metabolism during pressure overload-induced HF.Methylated RNA immunoprecipitation sequencing(MeRIP-seq)was performed to map transcriptome-wide m6A modifications in transverse aortic constriction(TAC)and sham hearts.The number of identified m6A peaks was higher in the TAC than in the sham hearts(Fig.1a).Consistent with previous reports,the m6A peaks were enriched in 3’-untranslated regions in both the TAC and sham hearts(Fig.1b).2 The m6A peaks were characterized by the canonical RGAAR(R=A or G)motif(Fig.1c). | Beijian Zhang Hao Jiang Jian Wu Yun Cai Zhen Dong Yongchao Zhao Qinfeng Hu Kai Hu Aijun Sun Junbo Ge | 2021 | Signal Transduction and Targeted Therapy2021，6，12： | 6 |
| 17 | Preparation and characterization of Al/B/Fe_2O_3 nanothermites显示文摘A sol-gel synthetic approach combined with an ultrasonic method was utilized to prepare Al/B/Fe2O3 nanothermites.The structure and properties of the prepared nanothermites were characterized by thermogravimetric analysis,differential scanning calorimetry,scanning electron microscopy,X-ray diffraction,and an impact sensitivity test.The results verified that the nano-aluminum and the micro-boron were uniformly dispersed in the pores of the iron oxide gel.The heat of the prepared Al/B/Fe2O3 nanothermites was 1.3 times that of the simple physically mixed sample.In addition,the heat of the combustion test showed that these materials were indeed energetic.Small-scale safe experiments also showed that the prepared materials through sol-gel were relatively insensitive to standard impact. | SHEN LianHua LI GuoPing LUO YunJun GAO Kun GE Zhen | 2014 | Science China Chemistry2014，57，6： | 6 |
| 18 | A phosphatidic acid-binding lncRNA SNHG9 facilitates LATS1 liquid–liquid phase separation to promote oncogenic YAP signaling显示文摘Long noncoding RNAs(lncRNAs)are emerging as a new class of important regulators of signal transduction in tissue homeostasis and cancer development.Liquid–liquid phase separation(LLPS)occurs in a wide range of biological processes,while its role in signal transduction remains largely undeciphered.In this study,we uncovered a lipid-associated lncRNA,small nucleolar RNA host gene 9(SNHG9)as a tumor-promoting lncRNA driving liquid droplet formation of Large Tumor Suppressor Kinase 1(LATS1)and inhibiting the Hippo pathway.Mechanistically,SNHG9 and its associated phosphatidic acids(PA)interact with the C-terminal domain of LATS1,promoting LATS1 phase separation and inhibiting LATS1-mediated YAP phosphorylation.Loss of SNHG9 suppresses xenograft breast tumor growth.Clinically,expression of SNHG9 positively correlates with YAP activity and breast cancer progression.Taken together,our results uncover a novel regulatory role of a tumor-promoting lncRNA(i.e.,SNHG9)in signal transduction and cancer development by facilitating the LLPS of a signaling kinase(i.e.,LATS1). | Rui-Hua Li Tian Tian Qi-Wei Ge Xin-Yu He Cheng-Yu Shi Jun-Hong Li Zhen Zhang Fang-Zhou Liu Ling-Jie Sang Zuo-Zhen Yang Ya-Zhuo Liu Yan Xiong Qingfeng Yan Xu Li Huai-Qiang Ju Jian Liu Liang-Jing Wang Jian-Zhong Shao Wenqi Wang Tianhua Zhou Aifu Lin | 2021 | Cell Research2021，31，10： | 6 |
| 19 | 成年大鼠睾丸经EDs处理后再生睾丸间质细胞基因图谱的改变显示文摘成年睾丸间质细胞(ALC)是睾丸中产生雄激素的唯一内分泌细胞,只占有睾丸细胞的1%-5%。大鼠睾丸中的睾丸间质细胞被EDs去除后能够再生。本研究中,我们主要研究了大鼠睾丸经EDS处理后睾丸间质细胞再生及基因的变化图谱。EDS处理后7、21、35、90天后分析血清中睾酮、睾丸基因图谱和一些类固醇生成相关的蛋白变化情况。EDS处理后第7天睾丸中睾酮水平几乎检测不到,然后开始恢复。处理后7天,81个基因下调2倍或者更多,其中48个基因检测不到,包括类固醇合成通路中的蛋白Star、Scarbl、Cypllal、Cypl7al、Hsd3bl、CyplblICyp2al。这些基因在21天开始表达增多,到90天已经完全恢复到正常水平。89个基因上调2倍或者更高,nPkib。这些上调的基因到90天恢复正常;其中Cyp2al直到35天才开始恢复,表明这个基因只在成年睾丸间质细胞中表达,不在前体细胞中表达。qPCR,Westernblot、半定量免疫组织染色检测了随机抽取的基因和蛋白确认其变化情况。 | Yu-Fei Zhang Kai-Ming Yuan Yong Liang Yan-Hui Chu Qing-Quan Lian Yu-Fei Ge Wei Zhen Chantal M Sottas Zhi-Jian Su Ren-Shan Ge | 2015 | Asian Journal of Andrology2015，17，2： | 5 |
| 20 | Alda-1 treatment promotes the therapeutic effect of mitochondrial transplantation for myocardial ischemia-reperfusion injury显示文摘Mitochondrial damage is a critical driver in myocardial ischemia-reperfusion(I/R)injury and can be alleviated via the mitochondrial transplantation.The efficiency of mitochondrial transplantation is determined by mitochondrial vitality.Because aldehyde dehydrogenase 2(ALDH2)has a key role in regulating mitochondrial homeostasis,we aimed to investigate its potential therapeutic effects on mitochondrial transplantation via the use of ALDH2 activator,Alda-1.Our present study demonstrated that time-dependent internalization of exogenous mitochondria by cardiomyocytes along with ATP production were significantly increased in response to mitochondrial transplantation.Furthermore,Alda-1 treatment remarkably promoted the oxygen consumption rate and baseline mechanical function of cardiomyocytes caused by mitochondrial transplantation.Mitochondrial transplantation inhibited cardiomyocyte apoptosis induced by the hypoxia-reoxygenation exposure,independent of Alda-1 treatment.However,promotion of the mechanical function of cardiomyocytes exposed to hypoxia-reoxygenation treatment was only observed after mitochondrial Alda-1 treatment and transplantation.By using a myocardial I/R mouse model,our results revealed that transplantation of Alda-1-treated mitochondria into mouse myocardial tissues limited the infarction size after I/R injury,which was at least in part due to increased mitochondrial potential-mediated fusion.In conclusion,ALDH2 activation in mitochondrial transplantation shows great potential for the treatment of myocardial I/R injury. | Xiaolei Sun Rifeng Gao Wenjia Li Yongchao Zhao Heng Yang Hang Chen Hao Jiang Zhen Dong Jingjing Hu Jin Liu Yunzeng Zou Aijun Sun Junbo Ge | 2021 | Bioactive Materials2021，6，7： | 5 |
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