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| 1 | Isolation and Sequence Analysis of NSP2, ORF3 and ORF5 of PRRSV XH-GD Strain from Guangdong Province显示文摘The diseased samples collected from Guangdong Province were inoculated to Marc-145 cells,PRRSV-specific cytopathogenic effects( CPE) such as cell aggregation,cell shrinkage and cell exfoliation were observed and a PRRSV strain XH-GD was obtained. By using primers designed in accordance with the published gene sequences of PRRSV in GenBank,NSP2,ORF3 and ORF5 fragments were amplified by reverse transcription polymerase chain reaction and analyzed by sequence alignment. The results demonstrated that PRRSV strain XH-GD belonged to the North American genotype and the NSP2 gene contained discontinuous deletions of 30 amino acids. NSP2,ORF3 and ORF5 genes of XH-GD shared 83. 3%- 98. 9%,88. 6%- 99. 2% and 88. 1%- 99. 2% nucleotide homology,and76. 8%- 98. 3%,83. 7%- 98. 8% and 88. 1%- 99. 2% amino acid homology with HUB1,NX06,BJsy06,VR-2332,HB-1( sh) 2002,HB-2( sh) 2002,CH-la and RespPRRS MLV,respectively; however,NSP2,ORF3 and ORF5 genes of XH-GD shared only 52. 9%,65. 0% and 64. 3% nucleotide homology,and31. 3%,57. 5% and 58. 9% amino acid homology with LV,respectively. Phylogenetic analysis revealed that XH-GD had a closer relationship with HUB1,NX06,BJsy06,VR-2332,HB-1( sh) 2002,HB-2( sh) 2002,CH-la and RespPRRS MLV strains compared with LV strain. | Zongxi CAO Quanhui PAN Zhemin LIN Liuwu KONG Yimin HE Guihong ZHANG | 2014 | Agricultural Biotechnology2014，3，1： | 2 |
| 2 | EM algorithm for estimating the noise deviation of the image in the wavelet domain 显示文摘 | Lin Zhemin Kang Xuelei Zhang Liming | 2004 | Journal of Infrared and Millimeter Waves2004，23，4： | 1 |
| 3 | 中国柯蚱属的研究及一新种记述(直翅目:蚱科)(英文)显示文摘系统记录中国柯蚱属的种类,以检索表形式进行分类研究,共记述柯蚱属31种,其中包括1新种,即短翅柯蚱Coptotettix brachypterus sp.nov.。文中附有柯蚱属名录和种类分布。 | ZHENG Zhemin LIN Liliang ZHANG Hongli 郑哲民 林立亮 张红利 | 2013 | Entomotaxonomia2013，35，1： | 1 |
| 4 | Effects of Compound Acidifier on Growth Performance and Serum Biochemical Parameters of Heat-stressed Wenchang Chickens显示文摘[Objective]The paper was to investigate the effects of compound acidifier on growth performance and serum biochemical parameters of heat-stressed Wenchang chickens.[Method]A total of 28881-day-old Wenchang chickens with the same genetic background and similar initial weight(985.63±20.35 g)were randomly divided into four groups,four replicates per group and 18 chickens per replicate.Birds were fed the basal diet supplemented with 0 g/kg(control group),1 g/kg(groupⅠ),2 g/kg(groupⅡ)and 3 g/kg(groupⅢ)compound acidifier.The trial lasted 35 d.[Result]The daily gain of experimental groupⅠwas significantly higher than those of control group and groupⅢ(P<0.05),and the feed intake of experimental groupⅠwas also markedly higher than that of control group(P<0.05),but the feed gain ratio of experimental groupⅠwas significantly lower than that of groupⅢ(P<0.05).Compared with control group,the serum total protein and albumin levels of experimental groupⅠincreased(P>0.05),but the serum uric acid concentration decreased(P>0.05).[Conclusion]Supplementing 1 g/kg compound acidifier could improve the growth performance of heat-stressed Wenchang chickens. | Liu Quanwei Gu Lihong Xing Manping Lin Dajie Liu Hailong Cao Zongxi Jiang Shouqun Lin Xiajing Lin Zhemin | 2020 | Animal Husbandry and Feed Science2020，12，2： | 0 |
| 5 | Study on Antibody Dynamics against Porcine Contagious Pleuropneumonia in Piglets显示文摘In order to provide basis for establishing a scientific and reasonable immune procedure against porcine contagious pleuropneumonia in pig farms,antibody dynamics in piglets inoculated with two vaccines against porcine contagious pleuropneumonia( APP-1,2,7) were detected. The result showed that average antibody blocking rate and antibody positive rate in piglets inoculated with two vaccines against porcine contagious pleuropneumonia exhibited similar variation trend. At 21 day after primary vaccination,average antibody blocking rate and antibody positive rate against porcine contagious pleuropneumonia in piglets reached the peak and then declined gradually. After secondary vaccination,average antibody blocking rate and antibody positive rate against porcine contagious pleuropneumonia in piglets increased gradually,which reached the second peak at 30 day after secondary vaccination( 60 day after primary vaccination); subsequently,average antibody blocking rate declined gradually but still remained at a high level; antibody positive rate had little change. Average antibody blocking rate against APP-1 was slightly lower than the critical level of antibody protection at 44 day after primary vaccination; antibody positive rate was slightly higher than the qualification rate at37 day after primary vaccination; average antibody blocking rate against APP-7 was close to the critical level of antibody protection at 30 day after primary vaccination,suggesting that piglets should be inoculated twice with polyvaccine against porcine contagious pleuropneumonia during the entire growth period,and the secondary vaccination should be performed at 30-37 day after primary vaccination. | Hailong LIU Yan ZHANG Lili HUANG Zongxi CAO Zhemin LIN | 2018 | Agricultural Biotechnology2018，7，1： | 0 |
| 6 | Preventive Effects of Five Drugs on Mycoplasma Pneumonia of Swine (MPS)显示文摘The paper was to explore the preventive effects of five drugs on mycoplasma pneumonia of swine (MPS) and to provide reference for clinical medication of pig farms in Hainan Province.A total of 444 health piglets were randomly divided into 6 groups,including five medication groups (72 piglets in group A,74 piglets in group B,72 piglets in group C,76 piglets in group D,76 piglets in group E) and one control group (74 piglets).The piglets in experimental groups were treated drugs once a day for successive 5 days at 30,60,90,120 and 150 of age.The piglets in control group were free of medication.At 70 and 140 days of age,15 piglets of each group were randomly selected to collect their blood serum.The Mycoplasma hyopneumoniae (M-Hyo) antibodies in serum were measured by enzyme-linked immunosorbent assay (ELISA).During the experiment,the incidence rates of respiratory disease,lung lesion,feed conversion rate,average daily gain (ADG),and mortality rate of pigs were also observed and recorded.The results showed that the five drugs had significant difference in preventative effects.Group C (Zhiyuanjing group) received the best preventive effect and the highest economic benefits.Compared with control group,the ADG and feed conversion rate in group C were increased by 7.53% and 9.09%,respectively; the incidence rate of respiratory disease was reduced by 13.44% and lung lesion was alleviated by81.43%; and the earnings of each pig could rise by 132.70 yuan.The preventative effect and economic benefit of the drugs was sequenced by Chansu Kechuanling and Bingchan Kechuanwang.Wante Feilin and amoxicillin had weaker preventive effects against MPS but greatly influenced growth performance of pigs,so they should be used alternatively with other drugs. | Zhang Yan Liu Hailong Lin Zhemin Cao Zongxi Tan Shuyi Chen Xiaojie Xie Yueshan | 2016 | Animal Husbandry and Feed Science2016，8，5： | 0 |
| 7 | Study on Growth and Decline Law of Contagious Pleuro-pneumonia in Piglets显示文摘In order to establish the immune procedure for Actinobacillus pleuropneumonia and to determine the date for the first immunization on piglets,the growth and decline law of A. pleuropneumonia material antibodies in the piglets borne by cows inoculated with A. pleuropneumonia vaccines( type I,Ⅱ,and Ⅶ) before delivery was detected. The results showed that type Ⅰ,Ⅱ,and Ⅶ maternal antibodies in piglets decreased gradually with the age growing overall,and was at the critical protection value at the ages of 42-50 days( type Ⅰ) and 28 days( type Ⅶ),lower than the quantification rate; and the antibodies all turned to be negative until the ages of 70 days( type Ⅰ),60 days( type Ⅶ) and 35 days( type Ⅱ). The first immunization should be carried out at the age of 42-50 days using type Ⅰ A. pleuropneumonia vaccine,and at the age of 28 days using type Ⅶ A. pleuropneumonia vaccine. However,type Ⅱ A. pleuropneumonia maternal antibody had lower level and positive rate and could not well protect piglets,so the various A. pleuropneumonia vaccines differed in the date for the first immunization. In order to achieve a better immunization effect,A. pleuropneumonia vaccines with different valences should be further researched and developed. | Yan ZHANG Hailong LIU Zhemin LIN Zongxi CAO Shuyi TAN | 2018 | Agricultural Biotechnology2018，7，5： | 0 |
| 8 | Phylogenetic Relationship Analysis of the Complete Genomes of Porcine Circovirus Type 2( PCV2) Strains Isolated from Hainan Province显示文摘[Objective] This study aimed to investigate the molecular characteristics of porcine circovirus type 2( PCV2) strains isolated from Hainan Province.[Method]The complete genome of PCV2 was amplified from PMWS-suspected samples by PCR for sequence analysis. [Result]A total of eight PCV2 strains were isolated and identified. All the eight isolates belonged to genotype PCV2 b,among which seven isolates belonged to subgenotype PCV2b-1C,and one isolate belonged to subgenotype PCV2b-1A /1B. ORF2 gene of PCV2 isolates from Hainan Province was 705 bp in length,encoding 234 amino acids. Antigenic epitopes of Cap protein exhibited certain changes. Nucleotide sequences and deduced amino acid sequences of ORF2 gene shared 95. 3%- 99. 7% and 93. 6%- 100% similarities among eight PCV2 isolates from Hainan Province,respectively. Moreover,nucleotide sequences and deduced amino acid sequences of ORF2 gene of PCV2 isolates from Hainan Province shared 91. 0%- 99. 9% and 91. 0%- 99. 6% similarities with other PCV2 strain isolated from China( AY682994,AF381175,JX945577,JX682407,AY180397),respectively; nucleotide sequences and deduced amino acid sequences of ORF2 gene of PCV2 isolates from Hainan Province shared 90. 0%- 97. 0% and 88. 0%- 97. 9% similarities with PCV2 isolates from other countries( NC_005148,JQ994268,KJ187306,AF201307,AF454546,AY754020),respectively; nucleotide sequences and deduced amino acid sequences of ORF2 gene of PCV2 isolates from Hainan Province shared 98. 2%- 100%and 94. 9%- 100% similarities with vaccine strain SH,respectively; nucleotide sequences and deduced amino acid sequences of ORF2 gene of PCV2 isolates from Hainan Province shared 90. 6%- 91. 7% and 89. 7%- 91. 0% similarities with vaccine strain LG,respectively. [Conclusion]This study provided theoretical basis for the prevention and control of PCV2 and selection of vaccine strains in Hainan Province. | Baoguo YE Xinli ZHENG Yan ZHANG Zhemin LIN Feng WANG Zongxi CAO | 2015 | Agricultural Biotechnology2015，4，5： | 0 |
| 9 | Isolation and PCR Identification of Duck Tembusu Virus显示文摘Duck tembusu virus disease is one of the most serious infectious diseases endangering duck industry. A strain of virus was isolated from a dead duck,and performed PCR identification and sequence analysis. The results showed that the sequence of the isolate shared above 99% homology with duck tembusu virus( DTMUV) sequence on Gen Bank. The result indicated that the isolated virus was DTMUV. | Huang Lili He Dongmei Ye Baoguo Zhang Yan Yang Shaoxiong Chen Chaoxi Lin Zhemin Cao Zongxi | 2017 | Animal Husbandry and Feed Science2017，9，5： | 0 |
| 10 | Cloning and Bioinformatics Analysis of TLR4 Gene from Wuzhishan Pig in Hainan显示文摘In order to obtain the TLR 4 gene sequence of Hainan Wuzhishan pig and analyze its molecular structure characteristics,its genomic sequence was cloned and sequenced by PCR and subjected to related bioinformatics analysis with the genomic sequence of the TLR 4 gene of pig in GenBank as a reference sequence.The full-length DNA sequence of Wuzhishan pig TLR 4 gene was 10 435 bp in length,of which CDS was 2 526 bp in length and encoded 841 amino acids.Compared with the common pig TLR 4 gene sequence published by GenBank,there were 38 mutations,of which two mutations were in the CDS region.The results of homology analysis showed that the amino acid sequence of the TLR 4 gene of Wuzhishan pig shared 99.9%,80.7%,79.9%,74.8%,74.7%,73.1%,73.0%,72.7%,62.5% and 43.4% homology with those of common pig,cattle,sheep,dog,horse,macaque,human,chimpanzee,mouse and chicken,respectively.The protein encoded by the TLR 4 of Wuzhishan pig belonged to secreted protein and transmembrane protein.It was hydrophilic,and had 8 glycosylation modification sites,17 serines (ser),7 threonines (Thr) and 8 tyrosine (TYR) phosphorylation sites.The amino acid phylogenetic tree analysis showed that the TLR 4 genes of different species are highly conserved during evolution.This result lays a foundation for further study of the function of TLR 4 gene. | Yan ZHANG Lili HUANG Hailong LIU Zhemin LIN Shuyi TAN | 2019 | Agricultural Biotechnology2019，8，5： | 0 |
| 11 | Advances of Studies on the Viral Proteins of PRRSV显示文摘Porcine reproductive and respiratory syndrome( PRRS) is one of viral diseases with severe reproductive obstacle of pregnant sows and respiratory tract symptoms and higher mortality of piglets as characteristics,which is caused by porcine reproductive and respiratory syndrome virus( PRRSV). PRRS has brought great threats to swine industry in the world. The advances of studies on the viral proteins of PRRSV were reviewed from the genome,non-structural proteins and structural proteins of PRRSV. | Cao Zongxi Shi Zhihai Lin Zhemin Jiao Peirong Zhang Guihong | 2014 | Animal Husbandry and Feed Science2014，6，2： | 0 |
| 12 | Comparison of Immune Effect of Four Commercial Marek's Disease Vaccines in Wenchang Chicken显示文摘Marek's disease(MD) is a lymphoproliferative disease of domestic chickens caused by a highly infectious,oncogenic alpha-herpesvirus known as Marek's disease virus(MDV).The aim of this study was to compare the efficacy of four commercial MDV vaccines in Wenchang chicken.The 1-day old Wenchang chickens tested were injected with one of four different vaccines or not unvaccinated as control;five days later,they were then challenged by virulent MDV strain MD5.The results showed that,in comparison with HVT vaccines,the CVI988 vaccine gave the immunized chickens more potent immunities against challenges of MDV strain MD5. | Cao Zongxi Zhang Yan Tan Shuyi Ye Baoguo Lin Zhemin | 2015 | Animal Husbandry and Feed Science2015，7，1： | 0 |
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