|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Soil Microbial Characteristics Under Long-Term Heavy Metal Stress:A Case Study in Zhangshi Wastewater Irrigation Area,Shenyang显示文摘Soil samples were collected from Zhangshi Wastewater Irrigation Area in the suburb of Shenyang City,China,an area with a 30-year irrigation history with heavy metal-containing wastewater.The chemical properties and microbial characteristics of the soils were examined to evaluate the present situation of heavy metal pollution and to assess the soil microbial characteristics under long-term heavy metal stress.In light of the National Environmental Quality Standards of China,the soil in the test area was heavily polluted by Cd and to a lesser degree by Zn and Cu,even though wastewater irrigation ceased in 1993.Soil metabolic quotient (qCO_2) had a significant positive correlation,while soil microbial quotient (qM) had a negative correlation with content of soil heavy metals.Soil microbial biomass carbon (MBC) had significantly negative correlation with Cd,but soil substrate-induced respiration (SIR),dehydrogenase activity (DHA),cellulase activity, and culturable microbial populations had no persistent correlations with soil heavy metal content.Soil nutrients,except for phosphorous,showed positive effects on soil microbial characteristics,which to a certain degree obscured the adverse effects of soil heavy metals.Soil Cd contributed more to the soil microbial characteristics,but qM and qCO_2 were more sensitive and showed persistent responses to heavy metals stress.It could be concluded that qM and qCO_2 can be used as bioindicators of heavy metal pollution in soils. | ZHANC Yan ZHANG Hui-Wen SU Zhen-Cheng ZHANG Cheng-Gang | 2008 | Pedosphere2008，18，1： | 12 |
| 2 | Elevated IL-37 levels in the plasma of patients with severe coronary arterycalcification显示文摘Background Coronary artery calcification (CAC) is a predictor of cardiovascular events and plaque burden and is closely associatedwith chronic inflammation. Intedeukin (IL)-37 is a newly discovered member of the IL-1 family and is considered an anti-inflammatorycytokine. Our recent study on mice indicated that IL-37 could attenuate atherosclerosis and vascular calcification, which suggests that IL-37could be associated with the development of atherosclerosis and related diseases. The aim of this study was to investigate if IL-37 plays arole in the progression of CAC in patients. Methods Two hundred participants with suspected cardiovascular disease were recruited. Thelevels of plasma IL-37, osteoprotegerin (OPG), hypersensitive C-reactive protein (hsCRP) together with other biochemical parameters weremeasured, and a coronary calcium assessment was carried out by multi-detector row CT. A score of 〈 10 AU (Agatston units) denotes anabsence of CAC, a score of 11-100 AU denotes mild CAC, 101-400 denotes moderate CAC, and 〉 400 AU denotes severe CAC. ResultsOur initial data showed that there were no apparent differences in plasma IL-37 levels among patients with or without mild or moderate CAC.However, IL-37 levels were significantly increased in patients with severe CAC (P 〈 0.001). Similar results were observed for plasma OPGand hsCRP levels. When IL-37 levels in patients with severe calcification were compared with that in all of the other non-severe CAC groups,it became apparent that there was a significant positive correlation between IL-37 level and severe CAC (r = 0.360, P 〈 0.001; OR = 1.033)using Spearrnan's correlation and binary logistic regression analysis. Conclusions This study demonstrates that the anti-inflammatory cy-tokine IL-37 is associated with high coronary calcium levels, suggesting that IL-37 expression may be caused by the activation ofinf/amma-tion and that IL-37 might become a predictor of severe CAC in the future, which requires further investigation. | Meng CHAI Hai-Tao ZHANG Yu-Jie ZHOU Qing-Wei JI Qing YANG Yu-Yang LIU Ying-Xin ZHAO Dong-Mei SHI Wei LIU Li-Xia YANG Lin-Lin ZHANC Jing LIANG | 2017 | Journal of Geriatric Cardiology2017，14，5： | 11 |
| 3 | Non-Fusion and Fusion Expression of β-Galactosidase from Lactobacillus bulgaricus in Lactococcus lactis显示文摘Objective To construct four recombinant Lactococcus lactis strains exhibiting high β-galactosidase activity in fusion or non-fusion ways,and to study the influence factors for their protein expression and secretion.Methods The gene fragments encoding β-galactosidase from two strains of Lactobacillus bulgaricus,wch9901 isolated from yogurt and 1.1480 purchased from the Chinese Academy of Sciences,were amplified and inserted into lactococcal expression vector pMG36e.For fusion expression,the open reading frame of the β-galactosidase gene was amplified,while for non-fusion expression,the open reading frame of the β-galactosidase gene was amplified with its native Shine-Dalgarno sequence upstream.The start codon of the β-galactosidase gene partially overlapped with the stop codon of vector origin open reading frame.Then,the recombinant plasmids were transformed into Escherichia coli DH5α and Lactococcus lactis subsp.lactis MG1363 and confirmed by determining β-galactosidase activities.Results The non-fusion expression plasmids showed a significantly higher β-galactosidase activity in transformed strains than the fusion expression plasmids.The highest enzyme activity was observed in Lactococcus lactis transformed with the non-fusion expression plasmids which were inserted into the β-galactosidase gene from Lactobacillus bulgaricus wch9901.The β-galactosidase activity was 2.75 times as high as that of the native counterpart.In addition,β-galactosidase expressed by recombinant plasmids in Lactococcus lactis could be secreted into the culture medium.The highest secretion rate(27.1%) was observed when the culture medium contained 20 g/L of lactose.Conclusion Different properties of the native bacteria may have some effects on the protein expression of recombinant plasmids.Non-fusion expression shows a higher enzyme activity in host bacteria.There may be a host-related weak secretion signal peptide gene within the structure gene of Lb.bulgaricus β-galactosidase,and its translation product may introduce the enzyme secretion out of cells in special hosts. | CHUAN WANG CHAO-WU ZHANC HENG-CHUAN LIU QIAN YU AND XIAO-FANG PEI | 2008 | Biomedical and Environmental Sciences2008，21，5： | 7 |
| 4 | Upregulation of MiR-126 Delays the Senescence of Human Glomerular Mesangial Cells Induced by High Glucose via Telomere-p53-p21-Rb Signaling Pathway显示文摘Diabetic kidney disease (DKD)is a microvascular complication of type 2 diabetes.The study of DKD mechanisms is the most important target for the prevention of DKD.Renal senescence is one of the important pathogeneses for DKD,but the mechanism of renal and cellular senescence is unclear.Decreased expression of circulating miR-126 is associated with the development of DKD and may be a promising blood-based biomarker for DKD.This study is to probe the effect and mechanism of miR-126 on the aging of human glomerular mesangial cells (HGMCs)induced by high glucose.HGMCs were cultured with Roswell Park Memorial Institute (RPMI-1640)in vitro.The effect of high glucose on morphology of HGMCs was observed 72h after intervention.The cell cycle was examined by flow cytometry.The telomere length was measured by Southern blotting.The expression levels of p53,p21 and Rb proteins in p53-p21-Rb signaling pathway and p-statl,p-stat3 in JAK/STAT signaling pathway were detected by Western blotting respectively.The expression of miR-126 was examined by qRT-PCR.MiR-126 mimics was transfected into HGMCs.The effects of miR-126 mimics transfection on cell morphology,cell cycle,telomere length,p53,p21,Rb,p-stat1 and p-stat3 were observed. The results showed that high glucose not only arrested the cell cycle in G1phase but also shortened the telomere length.High glucose led to high expression of p53,p21,Rb,p-statl and p-stat3 and premature senescence of HGMCs by activating the telomere-p53-p21-Rb and JAK/STAT signaling pathways.Moreover,the miR-126 was decreased in HGMCs induced by high glucose.It was suggested that the transfection of miR-126 mimics could inhibit the telomere-p53-p21-Rb and JAK/STAT signaling pathway activity in vitro and delay the senescence of HGMCs.The results may serve as a new strategy for the treatment of DKD. | Dong-wei CAO Chun-ming JIANG Cheng WAN Miao ZHANC Qing-yan ZHANG Min ZHAO Bo YANG Da-long ZHU Xiao HAN | 2018 | Current Medical Science2018，38，5： | 5 |
| 5 | Effects of natriuretic peptides on ventricular myocyte contraction and role of cyclic GMP signaling显示文摘 | Zhanc Q M0alem J Tse J | 2005 | Eur J Pharmcol2005，510，3： | 1 |
| 6 | Vanadium metabolism investigation using substance flow and scenario analysis显示文摘钒是一个重要战略资源,并且钒新陈代谢是中国的国家社会经济的系统的重要部分。这研究在中国在钒新陈代谢的生命周期上进行财务和情形分析。基于钒生命周期和物质流动分析(SFA ) 框架的特征,我们在场为一年 2010 的一个静态的人为的钒生命周期的量的评估。结果证明那人为的钒消费,股票,和新国内碎片分别地在 98.2, 21.2,和 4.1 kt;新碎片通常被丢弃。钒的全面利用比率是 32.2% 。大量钒是进跟踪,碎片,炉渣,和另外的花的固体的 stockpiled。情形分析被进行分析未来在中国的钒新陈代谢的发展趋势基于 SFA 框架和技术前进和社会经济的开发的质的分析。基线年被放作为 2010。几指示物被建议从 2010 ~ 2030 模仿不同情形。情形分析下一 20 年显示那是为在中国的钒工业的一个批评时期。这份报纸讨论在中国贡献持续的钒利用的改进的相关政策。 | Fangfang ZHANC Huiquan LI Bo CHEN Xue GUAN YiZHANG | 2014 | Frontiers of Environmental Science & Engineering2014，8，2： | 1 |
| 7 | On Einstein Kropina metrics显示文摘 | ZHANC X L SHEN Y B | 2013 | Differential Geometry and Its Applications2013，，31： | 1 |
| 8 | Cu/Ti3SiC2 composite: a new eleetrofriction material显示文摘 | ZHANC Y SUN Z M ZHOU Y C | 1999 | Mater Res lnnovat1999，，3： | 1 |
| 9 | ComparativestudyoftheperformanceoftheMcyclecounterflowandcrossflowheatexchangersforindirectevaporativecoolingPavingthepathtowardsustainablecoolingofbuildings显示文摘 | ZhanC DuanZ ZhaoX etal | 2011 | Energy2011，36，： | 1 |
| 10 | Multi-view imaging and 3DTV 显示文摘 | KUBOTA A SMOLIC A MAGNOR M TANIMOTO M CHEN T ZHANC C | 2007 | IEEE Signal Processing Magazine2007，11，4： | 1 |
| 11 | Study on ultrasonic vibration assisted grinding in theory 显示文摘 | ZHANC H L ZHANG J H | 2006 | Materials Science Forum2006，53，2533： | 1 |
| 12 | Estimation of glacier runoff and future trends in the Yangtze river source region, China显示文摘 | LIU Shi-yin ZHANG Yong ZHANC Yin-song | 2009 | Journal of Glaciology2009，55，190： | 1 |
| 13 | CSM communication apply in control of agriculture irrigation显示文摘 | ZHANC F YANG M YIN J | 2004 | Xinjiang Agriculture Technology2004，1，： | 1 |
| 14 | Endomorphin-1 analogs with enhanced metabolic stability and systemic analgesic activity: design, synthesis, and pharmacological characteri- zation显示文摘 | LIu Hong-mei ZHANC Bang-zhi LIu Xue-feng | 2007 | Bioorgan Med Chem2007，15，4： | 1 |
| 15 | Separation Control of Axial Compressor Cascade by Fluidic-Based Excitations 显示文摘 | ZHENG XinQian ZHANC YangJun XING WeiDong et al | 2011 | ASME Journal of turboma- chinery2011，133，04： | 1 |
| 16 | The enhancement of completely an- totrophic nitrogen removal over nitrite (CANON) by N2H4 addition显示文摘 | YAO Z B CAI Q ZHANC D J | 2013 | Bioresource Technology2013，146，： | 1 |
| 17 | A new data clustering algorithm and its application显示文摘 | RAMAKRISHNAN R LIVNY M | 1997 | Data Mining and Knowledge Discovery1997，1，2： | 1 |
| 18 | Preparation of L-phenylalanine imprinted polymer based on monodisperse hybrid silica microsphere and its application on chiral separation of phenylalanine racemates as HPLC stationary phase 显示文摘 | ZHANC Zhao-hui ZHANG Ming-lei LIU Yu-nan | 2012 | Separation and Purification Technology2012，87，： | 1 |
| 19 | Symhesis of an amino-terminated hyperbranched polymer and its application in reactive dyeing on cot- ton as a salt-free dyeing auxiliary显示文摘 | ZHANC F CHEN Y LIN H | 2007 | Coloration Technology2007，123，： | 1 |
| 20 | Influenee of two systematic parameters on thegeothermal heat pump system operation显示文摘 | L ZHAO T J ZHANG Q ZHANC | 2003 | Renewable Energy2003，28，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员