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| 1 | Acclimatization of in Vitro-derived Dendrobium显示文摘The successful ex vitro establishment of Dendrobium plantlets raised in vitro determines the quality of the end product(cut flowers or potted plants) in commercial production for economic gain. When in vitro Dendrobium plantlets are transplanted from the culture room to greenhouse conditions, they may desiccate or wilt rapidly and can die as a result of changes in the environment, unless substantial precautions are taken to adapt plantlets to a new environment. The acclimatization of in vitro-grown Dendrobium plantlets to an ex vitro environment by gradually weaning them towards ambient relative humidity and light levels facilitates better survival of young and physiologically sensitive plantlets.Dendrobium plantlets raised in vitro must thus undergo a period of acclimatization or transitional development to correct anatomical abnormalities and to enhance their physiological performance to ensure survival under ex vitro conditions. The most common approach to improve the survival of Dendrobium plantlets upon transfer to an ex vitro environment is their gradual adaptation to that environment. Under such conditions, plants convert rapidly from a heterotrophic or photomixotrophic state to an autotrophic growth, develop a fully functional root system,and better control their stomatal and cuticular transpiration. Gradual adaptation is carried out in a greenhouse by decreasing relative humidity using fog or mist chambers and by increasing light intensity using shading techniques. This review details the acclimatization and ex vitro survival of Dendrobium plants produced in vitro. This advice is also useful for other orchids. | Jaime A.Teixeira da Silva Mohammad Musharof Hossain Madhu Sharma Judit Dobránszki Jean Carlos Cardoso ZENG Songjun | 2017 | Horticultural Plant Journal2017，3，3： | 4 |
| 2 | Symmetry-adapted spherical harmonics method for high- resolution 3D single particle reconstructions显示文摘 | Liu Hongrong Cheng Lingpeng Zeng Songjun | 2008 | Journal of Structural Biology2008，161，1： | 1 |
| 3 | Intense blue photo-luminescence of the Tm3+/Yb3+co-doped single-crystallinehexagonal phase NaYF4nanorods?显示文摘 | Zeng Songjun Ren Guozhong Xu Changfu | 2011 | J Alloy Compd2011，509，5： | 1 |
| 4 | Intense blue photoluminescence of the Tm 3+ /Yb 3+ co-doped single-crystalline hexagonal phase NaYF 4 nanorods显示文摘 | Songjun Zeng Guozhong Ren Changfu Xu | 2010 | Journal of Alloys and Compounds2010，，5： | 1 |
| 5 | Photoinitiator grafted styrene-butadiene-styrene triblock copolymer显示文摘 | Guoliang Wu Songjun Zeng Encai Ou | 2010 | Materials Science and Engineering:C2010，30，： | 1 |
| 6 | Upconversion luminescence and magnetic properties of ligand-free monodisperse lanthanide doped BaGdF 5 nanocrystals显示文摘 | Changfu Xu Mo Ma Liwen Yang Songjun Zeng Qibin Yang | 2011 | Journal of Luminescence2011，，12： | 1 |
| 7 | Direct somatic embryogenesis and related gene expression networks in leaf explants of Hippeastrum ‘Bangkok Rose’显示文摘Hippeastrum, a highly diverse genus in the Amaryllidaceae family, is a valuable ornamental bulbous flowering plant. Somatic embryogenesis(SE) is an efficient method for mass production of Hippeastrum plantlets. Previous studies have been devoted to the in vitro propagation of Hippeastrum, but the SE and its regulatory networks are rarely reported. In this study, we established a direct SE method of Hippeastrum Bangkok Rose' using leaf bases as explants. MS supplemented with 1.00 mg·L^(-1)NAA +1.00 mg·L^(-1)KT + 0.25 mg·L^(-1)TDZ was the optimal medium for SE. Histological observations showed that the bipolar somatic embryo originated from the epidermal cell layer and underwent initiation,globular, scutellar and coleoptile stages. During SE, endogenous hormones of IAA, CTK, ABA, and SA were highly accumulated. Transcriptomic analysis revealed the genes encoding auxin biosynthesis/metabolic enzymes and efflux carriers were induced, while the auxin receptor of TIR1 and ARF transcriptional repressor of Aux/IAA were down-regulated and up-regulated, respectively, leading to suppression of auxin signaling. In contrast, cytokine signaling was promoted at the early stage of SE, as biosynthesis, transport, and signaling components were up-regulated.Various stress-related genes were up-regulated at the early or late stages of SE. Chromatin remodeling could also be dynamically regulated via distinct expression enzymes that control histone methylation and acetylation during SE. Moreover, key SE regulators, including WOXs and SERKs were highly expressed along with SE. Overall, the present study provides insights into the SE regulatory mechanisms of the Hippeastrum. | Jingjue Zeng Yi Deng Shahid Iqbal Jiarui Zhang Kunlin Wu Guohua Ma Lin Li Guangyi Dai Rufang Deng Lin Fang Songjun Zeng | 2024 | Horticultural Plant Journal2024，10，2： | 0 |
| 8 | In vivo optical bioimaging by using Nd-doped LaF3 luminescent nanorods in the second near-infrared window显示文摘Fluorescent imaging in the second near-infrared(NIR-Ⅱ,1000-1700 nm)region has attracted enormous research interest due to its advantages of unprecedentedly improved imaging sensitivity and spatial resolution.Herein,high quality NIR-Ⅱemissive LaF3:Nd3+nanorods(NRs)with hexagonal phase structure and controlled size were synthesized by a hydrothermal method.And the Nd^3+doping induced NIRⅡluminescent intensity evolution was studied,In vivo NIR-Ⅱbioimaging and real-time tracking reveal that these LaF3:Nd^3+NRs are mainly accumulated in liver and spleen.Moreover,in vivo toxicity assessment demonstrates that LaF3:Nd^3+NRs feature low biotoxicity and good biocompatibility in living animals.Therefore,the NIR-Ⅱemitting hexagonal phase LaF3 NRs with controlled size are promising probes for optical bioimaging. | Shouyan Cheng Liu Liu Qiuhua Yang Youbin Li Songjun Zeng | 2019 | Journal of Rare Earths2019，37，9： | 0 |
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