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您的检索式：作者名="Zehra Hashim"
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| 1 | Therapeutic effect of hydrogen peroxide via altered expression of glutathione S-transferase and peroxiredoxin-2 in hepatocellular carcinoma显示文摘Background:Hepatocellular carcinoma(HCC)has a high incidence and mortality that epitomizes one of the prominent causes of cancer-related death globally.Novel therapeutic approaches are therefore required.Reactive oxygen species(ROS)are necessary for maintaining cell cycle.Although ROS is involved in HCC progression,hydrogen peroxide(H2O2)has anti-proliferative effect on HCC.Method:HCC Huh-7 cells were cultured and incubated with various concentrations of H2O2.Paraoxonase activity,levels of malondialdehyde,glutathione and protein oxidation were measured in treated and untreated Huh-7 cells.Furthermore,untreated and treated Huh-7 cells were subjected to two dimensional gel electrophoresis and identified protein spots which were differentially expressed by LC-MS/MS analysis.q RT-PCR was performed to validate the identified proteins.Results:H2O2 depleted glutathione(GSH)with the concomitant up-regulation of GSTP1 and Prx2.H2O2 also increased malondialdehyde and protein oxidation,decreased the activity of paraoxonase in Huh-7 cells.Conclusion:H2O2 could be used as a novel therapeutic agent that might be beneficial in inducing cell cytotoxicity and hence suppress HCC proliferation. | Zehra Hashim Amber Ilyas Shamshad Zarina | 2020 | Hepatobiliary & Pancreatic Diseases International2020，19，3： | 1 |
| 2 | Osmotic stress induced oxidative damage: Possible mechanism of cataract formation in diabetes显示文摘 | Zehra Hashim Shamshad Zarina | 2012 | Journal of Diabetes and Its Complications2012，，4： | 1 |
| 3 | Alendronate and FTI-277 combination as a possible therapeutic approach for hepatocellular carcinoma:An in vitro study显示文摘Background: An important product of mevalonate pathway is downstream synthesis of isoprenoid units that has long been implicated in development and progression of tumor. It has been speculated that inhibition of protein prenylation might be therapeutically beneficial. The objective of current study was to evaluate antitumor potential of a novel therapeutic combination of mevalonate pathway inhibitors, FTI-277 and alendronate. We also examined differentially expressed proteins in response to treatment using proteomics approach.Methods: Huh-7 cells were incubated with different concentrations of FTI-277 alone and in combination with alendronate. Differential protein and gene expression was examined through two dimensional gel electrophoresis and real-time quantitative polymerase chain reaction(q PCR), respectively. Proteins were identified using tandem mass spectrometry analysis.Results: Treatment of hepatocellular carcinoma(HCC) cell line with FTI-277 alone showed cell death in a time and dose dependent manner while in combination with alendronate, a synergistic apoptotic effect at 24 h was observed. Proteomic studies on the 20 μmol/L FTI-277 and 5 μmol/L alendronate +20 μmol/L FTI-277 treated cells revealed altered expression of different proteins including peroxiredoxin 2(Prx2),glutathione S transferase 1(GSTP1), Rho GTPase activating protein(Rho GAP), triosephosphate isomerase(TPI), and heat shock protein 60(HSP60). Down-regulated expression of Prx2 and GSTP1 in treated cells was also confirmed by real-time q PCR analysis.Conclusions: Combined treatment of FTI-277 and alendronate on Huh-7 HCC cells showed cell death suggesting their anticancer potential. Such treatment approaches are likely to offer new therapeutic strategies. | Amber Ilyas Zehra Hashim Iffat Saeed Channa Shamshad Zarina | 2018 | Hepatobiliary & Pancreatic Diseases International2018，17，3： | 0 |
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