|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Magnetic resonance evaluation of transplanted mesenchymal stem cells after myocardial infarction in swine显示文摘Objectives To trace and evaluate intracoronary transplanted mesenchymal stem cells(MSCs) labeled with superparamagnetic iron oxide(SPIO) by using magnetic resonance imaging(MRI) in a swine model of myocardial infarction (MI).Methods MSCs were transfected with a lentiviral vector carrying the gene encoding green fluorescent protein (GFP) and labeled in vitro with SPIO.Two weeks after MI, swine were randomized to intracoronary transplantation of dual -labeled MSCs(n = 10),MSCs-GFP(n = 10) and saline(n = 5).MRI examination was performed with a 1.5T clinical scanner at 24 hours,3 weeks and 8 weeks after cells transplantation. Signal intensity(SI) changes,cardiac function and MI size were measured using MRI.Correlation between MR findings and histomorphologic findings was also investigated. Results The labeling efficiency at a combination of 25μg Fe/ml SPIO and 0.8 pi/ml Lipofectamine 2000 reached 100%.SPIO labeling did not affect GFP fluorescence and dual-labeling did not affect cell proliferation(P>0.05). Multipotentiality was not affected especially for cardiomyocyte-like cells differentiation.Cardiac cell marker of a-MHC and actinin were positively expressed by immunofluorescence staining after induction.SI on T2 * WI decreased substantial- ly in the interventricular septum 24 hours after injection of MSCs.The intensity of hypo-intense signals appeared to increase throughout the later time points.Changes in SI at 24 hours,3 weeks and 8 weeks were 52.98%±10.74%,21.53%±5.40%and 6.23%±2.01%,respectively(P<0.01).DE-MRI demonstrated both dual-labeled MSCSs and MSCs-GFP could dramatically reduce the size of MI and improve cardiac function. Histological data revealed that prussian blue stain-positive cells were found mainly in the border zone which also showed green fluorescence but negative for macrophage marker(CD68).Gross pathologic examination revealed that engrafted MSCs dramatically reduce the extent of necrotic myocardium and promote the regeneration of new,contractile myocardium along the subendocardial surface of the MI. Conclusions MSCs could be efficiently and safely labeled with SPIO and GFP,and could be detected reproducibly and noninvasively in vivo using cardiac MRI.Intracoronary transplantation of dual-labeled MSCs could increase cardiac function and reduce the size of MI. | KE Yang~1,PENG Chao-quan~1,XIANG Peng~1,ZHANG Cheng-xi~1, ZOU Li-yuan~1,WU Xiao~1,YA Gao~1,KANG Z huang~2,HE Ke-ke~2,LIU Jin-lai~1 (1.Department of Cardiology,The Third Affiliated Hospital, Sun Yat-sen University,Guangzhou 2.Department of Radiology, the Third Affiliated Hospital,Sun Yat-sen University, Guangzhou 5100630 3.Center for Stem Cell Biology and Tissue Engineering,Sun Yat-sen University,Guangzhou 5100120) | 2011 | 岭南心血管病杂志2011，17，S1： | 7 |
| 2 | High-pressure high-temperature X-ray diffraction of β-boron to 30GPa显示文摘 | Ma Y Z Prewitt C T Zou G T | 2003 | Phys Rev B2003，67，17： | 1 |
| 3 | Effect of β-mannanase (Hemicell) on growth performance and immunity of broilers显示文摘 | Zou X T Qiao X J Xu Z R | 2006 | Poult Sci2006，85，12： | 1 |
| 4 | Binding of puerarin to human serum albumin:a spectroscopic analysis and molecular docking显示文摘 | HE Y WANG Y W TANG L F LIU H CHEN W ZHENG Z L ZOU G L | 2008 | J Fluoresc2008，18，2： | 1 |
| 5 | The addition of toeilizumab to DMARD therapy for rheumatoid arthritis: a meta-analysis of randomized controlled trials显示文摘 | An MM Zou Z Shen H | 2010 | Eur J Clin Pharmacol2010，66，1： | 1 |
| 6 | Photocatalytic decomposition of organic contaminants by Bi2WO6 under visible light irradiation显示文摘 | TANG J W ZOU Z G YIE J H | 2004 | Catal Lett2004，92，12： | 1 |
| 7 | Severe dendritic cell perturbation is actively involved in the pathogenesis of acute-on-chronic hepa- titis B liver failure显示文摘 | Zhang Z Zou ZS Fu JL | 2008 | J Hepatol2008，49，3： | 1 |
| 8 | Prevalen of idiopathic hypertrophic cardiomyopathy in China: a population-based echocardiographic analysis of 8080 adults 显示文摘 | Zou Y Song L Wang Z | 2004 | Am J Med2004，116，1： | 1 |
| 9 | Photocatalytic decomposition of organic contaminants by Bi2WO6 under visible light irradiation 显示文摘 | Tang J Zou Z Ye J | 2004 | Catalysis Letters2004，92，12： | 1 |
| 10 | Down-regulation of RhoE is associated with progression and poor prognosis in hepatocellular carcinoma显示文摘 | Luo H Dong Z Zou J | 2012 | J Surg Oncol2012，105，7： | 1 |
| 11 | Abiogenic gas: should the carbon isotope order be reversed? 显示文摘 | Wei Z F Zou Y R Cai Y L | 2012 | Journal of Petroleum Science and Engineering2012，84，85： | 1 |
| 12 | Distinct roles of isoforms of the heme-liganded nuclear receptor E75, an insect ortholog of the vertebrate Rev-erb, in mosquito reproduction 显示文摘 | Cruz J Mane-Padros D Zou Z | 2012 | Molecular and Cellular Endocrinology2012，349，2： | 1 |
| 13 | Cancer statistics, 2014 显示文摘 | Siegel R Ma J Zou Z | 2014 | CA Cancer J Clin2014，64，： | 1 |
| 14 | Photocatalytic decomposition of organic contaminants by Bi2WO6 under visible light ir- radiation 显示文摘 | Tang J W Zou Z G Ye J H | 2004 | Catal Lett2004，92，12： | 1 |
| 15 | Detection of Fabric Defects with Fuzzy Label Co-occurrence Matrix Set显示文摘 | Zou C Wang B W Sun Z G | 2009 | Journal of Donghua University(English Version)2009，26，5： | 1 |
| 16 | A semi-fragile lossless digital watermarking scheme based on integer wavelet transform 显示文摘 | Zou D SHX Y Q NI Z C | 2006 | IEEE Transactions on Circuits Systems for Video Technology2006，16，10： | 1 |
| 17 | Sensitive and rapid detection of the new Delhi metallo-beta- lactamase gene by loop-mediated isothermal amplification 显示文摘 | Liu W Zou D Li Y Wang X He X Wei X Shao C Li X Shang W Yu K Liu D Li Y Guo J Yin Z Yuan J | 2012 | J Clin Microbiol2012，50，5： | 1 |
| 18 | Finite-time attitude tracking control for spacecraft using terminal sliding model and chebyshev neural network显示文摘 | ZOU A KUMAR K D HOU Z | | 0，，： | 1 |
| 19 | Cancer statistics, 2014 显示文摘 | Siegel R Ma J Zou Z | 2014 | CA Cancer J Clin2014，64，1： | 1 |
| 20 | Cancer Statistics, 2014显示文摘 | Siegel R Ma J Zou Z | 2014 | CA Cancer J Clin2014，64，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员