2篇
您的检索式：作者名="ZOU Guiwei"
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| 1 | Design of Vibrio 16S rRNA Gene Specific Primers and Their Application in the Analysis of Seawater Vibrio Community显示文摘类 Vibrio 原因 vibriosis 的病原的种, maricultured 动物和海味消费者的最流行的疾病之一。在海味生产的链监视他们的动力学,处理并且消费为食物和海洋生物养殖安全是很重要的。为了充实代表 Vibrio 16S ribosomal RNA 基因(rDNA ) ,碎裂并且识别进一步真实及时的这些细菌并且同时地在在链的细菌的植物群之中,有选择地放大 Vibrio 16S rDNA 碎片的一双教材与他们在海水 Vibrio 的分析证明的特性和报道被设计社区。二份教材, VF169 和 VR744 的 Thespecificities 和范围,被使用强风节目在在 GenBank 可得到的细菌的 16S rDNAs 之中理论上决定,实际上,由放大 Vibrio16S, rDNA 从海水 DNA 碎裂。超过 88.3% 序列在 GenBank,与 VR744 显示出相同火柴,属于 Vibrio 类。33 克隆的一个总数随机被选择并且定序。所有序列显示出他们的最高的类似到并且多样的已知的 Vibrio 种类在那些附近聚类。设计的教材能够检索宽范围 ofVibrio 16S rDNA 在海水在细菌的植物群之中明确地碎裂,海味耕作的最重要的自然环境。 | LIU Yong YANG Guanpin WANG Hualei CHEN Jixiang SHI Xianming ZOU Guiwei WEI Qiwei SUN Xiuqin | 2006 | Journal of Ocean University of China2006，5，2： | 5 |
| 2 | Cloning and Expression Analysis of a Prion Protein Encoding Gene in Guppy (Poecilia reticulata)显示文摘The full length cDNA of a prion protein (PrP) encoding gene of guppy (Poecilia reticulata) and the corresponding ge-nomic DNA were cloned. The cDNA was 2245 bp in length and contained an open reading frame (ORF) of 1545 bp encoding a pro-tein of 515 amino acids,which held all typical structural characteristics of the functional PrP. The cloned genomic DNA fragment corresponding to the cDNA was 3720 bp in length,consisting of 2 introns and 2 exons. The 5’ untranslated region of cDNA origi-nated from the 2 exons,while the ORF originated from the second exon. Although the gene was transcribed in diverse tissues in-cluding brain,eye,liver,intestine,muscle and tail,its transcript was most abundant in the brain. In addition,the transcription of the gene was enhanced by 5 salinity,implying that it was associated with the response of guppy to saline stress. | WU Suihan WEI Qiwei YANG GuanDin WANG Dengqiang ZOU Guiwei CHEN Daqing | 2008 | Journal of Ocean University of China2008，7，4： | 1 |
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