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| 1 | Identification of Cold Tolerance of Rice Germplasm Resource at Germinating Stage in Cold Region显示文摘Total 75 rice varieties(lines) in Heilongjiang Province(or cold region) as germplasm resources were identified for cold tolerance at germinating stage by controlling temperature in artificial incubator.The results showed that the shooting seed rate at the germinating stage could be used as the evaluation index of cold tolerance.The cold tolerance was recorded on 1-9 scale and could be identified by the criteria of five indexes such as highly tolerant(HT),tolerant(T),moderately tolerant(MT),susceptible(S),highly susceptible(HS). | LIU Hualong SUN Shiche WANG Jingguo ZOU Detang | 2008 | Journal of Northeast Agricultural University(English Edition)2008，15，2： | 6 |
| 2 | OsADR3 increases drought stress tolerance by inducing antioxidant defense mechanisms and regulating OsGPX1 in rice(Oryza sativa L.)显示文摘The C (Cys) 2H (His) 2-type transcription factor is one of the most important transcription factors in plants and plays a regulatory role in the physiological responses of rice to abiotic stresses.A novel rice C2H2-type zinc finger protein,abscisic acid (ABA)-drought-reactive oxygen species (ROS) 3 (OsADR3),was found to confer drought stress tolerance by enhancing antioxidant defense and regulating Os GPX1.Overexpression of OsADR3 in rice increased tolerance to drought stress by increasing ROS scavenging ability and ABA sensitivity.In contrast,CRISPR/Cas9-mediated knockout of osadr3 increased the sensitivity of rice to drought and oxidative stress.An exogenous ROS-scavenging reagent restored the droughtstress tolerance of osadr3-CRISPR plants.Global transcriptome analysis suggested that OsADR3 increased the expression of Os GPX1 under drought stress.Electrophoretic mobility shift,yeast one-hybrid,and dualluciferase reporter assays revealed that OsADR3 modified the expression of Os GPX1 by directly binding to its promoter.Knockdown of Os GPX1 repressed ROS scavenging ability under drought stress in OsADR3-overexpression plants.These findings suggest that OsADR3 plays a positive regulatory role in droughtstress tolerance by inducing antioxidant defense and associated with the ABA signaling pathway in rice. | Jiaming Li Minghui Zhang Luomiao Yang Xinrui Mao Jinjie Li Lu Li Jingguo Wang Hualong Liu Hongliang Zheng Zichao Li Hongwei Zhao Xianwei Li Lei Lei Jian Sun Detang Zou | 2021 | The Crop Journal2021，9，5： | 2 |
| 3 | Study on Difference in Cold Tolerance Between Weedy Rice and Cultivated Rice in Heilongjiang Province of China显示文摘A long-red awn weedy rice and rice cultivars named Ken99004(ZC1), Crossing-503(ZC2), Ken Sticky Rice(ZC3), Shashani(ZC4), Long-Grain Aromatic Rice(ZC5) were used to study typical wild traits of weedy rice and to identify the cold resistance of all genotypes. The results showed that the stem of long-red awn weedy rice was the weakest. No difference was found in the early stage of rice growth, e.g. the vegetable growth stage, between long-red awn weedy rice and other rice cultivars in the stem rigidity, but in the later stage, or reproductive growth stage, their leaves and stems were senescent rapidly because of the speedy supply of nutrients for panicle growth, meanwhile the rigidity of stem was reduced sharply just as withered weeds. The germination rate of longred awn weedy rice was the highest in cold condition, and in turn were ZC1, ZC2, ZC3, ZC4, ZC5, respectively. The performances of genotypes in cold tolerance were identical, those with a high ability of germination in low temperature also showed a strong cold tolerance in main traits in whole growth period, the order from strong to weak in the extent of cold tolerance were long-red awn weedy rice, ZC1, ZC2, ZC3, ZC4, ZC5, respectively. | ZOU Detang GUO Junxiang LIU Hualong WANG Jingguo | 2008 | Journal of Northeast Agricultural University(English Edition)2008，15，1： | 1 |
| 4 | Elite sd1 alleles in japonica rice and their breeding applications in northeast China显示文摘The Green Revolution gene sd1 has been used extensively in modern rice breeding,especially in indica cultivars.However,elite sd1 alleles and related germplasm resources used for japonica rice breeding have not been identified,and extensive efforts are needed for japonica rice breeding to obtain new dwarfing sources.Data from MBKbase-Rice revealed seven sd1 haplotypes in indica and four in japonica rice.Two new sd1 alleles were identified in indica rice.In 295 japonica accessions from northeast Asia,except for the weak functional allele SD1-EQ,sd1-r was the major allele,reducing plant height in comparison with SD1-EQ.Japonica germplasm resources carrying reported sd1 alleles were identified by genotype searching and further verified by literature search,genealogical analysis,and d Caps markers.Pedigrees and geographic distribution showed that sd1-r is an excellent allele widely used in northern China and Tohoku in Japan,and sd1-j is commonly used in east China and Kyushu in Japan.Dongnong-and Xiushui-series cultivars carrying sd1-r and sd1-j,respectively,are essential branches of the backbone parents of Chinese japonica rice,Akihikari and Ce21,with the largest number of descendants and derived generations.In semi-dwarf japonica rice breeding,sd1-d was introgressed into Daohuaxiang 2(DHX2).Dwarf and semi-dwarf lines carrying sd1-d were selected and designated as 1279 and 1280,respectively,after withstanding typhoon-induced strong winds and heavy rains in 2020,and are anticipated to become useful intermediate materials for future genetic research and breeding.This work will facilitate the introduction,parental selection,and marker-assisted breeding,and provide a material basis for the next step in identifying favorable genes that selected together with the sd1 alleles in japonica backbone parents. | Hanjing Sha Hualong Liu Guangxin Zhao Zhongmin Han Huilin Chang Jingguo Wang Hongliang Zheng Jifeng Zhang Yang Yu Yuqiang Liu Detang Zou Shoujun Nie Jun Fang | 2022 | The Crop Journal2022，10，1： | 0 |
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