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| 1 | Reappraisement and refinement of zircon U-Pb isotope and trace element analyses by LA-ICP-MS显示文摘A protocol was established for simultaneous measurements of zircon U-Pb ages and trace elements by LA-ICP-MS at spot sizes of 16 32 μm.This was accomplished by introducing N 2 into ICP to increase the sensitivity.The obtained U-Pb ages for zircon standards GJ-1,TEMORA and SK10-2 are consistent with the preferred values within about 1% uncertainty (2σ) by simple external calibration against zircon standard 91500.Different data reduction softwares could yield different uncertainties for calculation of U-Pb ages.The commercially available program GLITTER4.4 could apply an improper uncertainty calculation strategy,but it may yield artificial high precisions for single analyses.Our trace element analyses indicate that Si is not an ideal internal standard for zircon when calibrated against the NIST glasses.Calibration against the NIST glasses using Si as an internal standard,a systematic deviation of 10% 30% was found for most trace elements including Zr.However,the trace element compositions of zircon can be accurately measured by calibration against multiple reference materials with natural compositions (e.g.,BCR-2G,BHVO-2G and BIR-1G),or calibration against NIST SRM 610 and using Zr as an internal standard.Analyses of two pieces of GJ-1 demonstrate that it is relatively homogenous for most trace elements (except for Ti). | LIU YongSheng HU ZhaoChu ZONG KeQing GAO ChangGui GAO Shan XU Juan CHEN HaiHong | 2010 | Chinese Science Bulletin2010，55，15： | 1011 |
| 2 | 激光剥蚀信号在线平滑和去汞装置:原位微区铅同位素准确分析利器显示文摘激光原位微区分析可在微观尺度上完成样品的元素定量分析或同位素组成测定,为研究成岩、成矿物质来源和追踪地壳与地幔演化历史提供了独特的观察视野。但是,激光剥蚀等离子体质谱的一些缺陷阻碍了地质样品的高精度、高空间分辨率分析,比如:剥蚀池的位置效应、低剥蚀速率下信号波动影响、激光剥蚀脉动式信号导致的光谱螺纹效应、高频激光剥蚀造成的元素分馏效应等。为此,一些学者设计了不同规格和样式的激光剥蚀信号平滑装置来克服这些问题。但是,这些信号平滑装置普遍还存在气体吹扫时间过长和降低激光剥蚀信号灵敏度等问题。因此,研制新型的信号平滑装置对改善LA—ICP—MS的数据质量具有重要的意义。 | Hu Z Zhang W Liu Y Gao S Li M Zong K Chen H Hu S 刘勇胜 | 2015 | 矿物岩石地球化学通报2015，34，4： | 114 |
| 3 | Current cancer situation in China:good or bad news from the 2018 Global Cancer Statistics?显示文摘Cancer is the leading cause of death in China and depicting the cancer pattern of China would provide basic knowhows on how to tackle it more effectively.In this study we have reviewed several reports of cancer burden,including the Global cancer statistics 2018 and Cancer statistics in China,2015,along with the GLOBCAN 2018 online database,to investigate the differences of cancer patterns between China,the United States(USA)and the United Kingdom(UK).An estimated 4.3 million new cancer cases and 2.9 million new cancer deaths occurred in China in 2018.Compared to the USA and UK,China has lower cancer incidence but a 30%and 40%higher cancer mortality than the UK and USA,among which 36.4%of the cancer-related deaths were from the digestive tract cancers(stomach,liver,and esophagus cancer)and have relatively poorer prognoses.In comparison,the digestive cancer deaths only took up≤5%of the total cancer deaths in either USA or UK.Other reasons for the higher mortality in China may be the low rate of early-stage cancers at diagnosis and non-uniformed clinical cancer treatment strategies performed by different regions.China is undergoing the cancer transition stage where the cancer spectrum is changing from developing country to developed country,with a rapidly increase cancer burden of colorectal,prostate,female breast cancers in addition to a high occurrence of infection-related and digestive cancers.The incidence of westernized lifestyle-related cancers in China(i.e.colorectal cancer,prostate,bladder cancer)has risen but the incidence of the digestive cancers has decreased from 2000 to 2011.An estimated 40%of the risk factors can be attributed to environmental and lifestyle factors either in China or other developed countries.Tobacco smoking is the single most important carcinogenic risk factor in China,contributing to~24.5%of cancers in males.Chronic infection is another important preventable cancer contributor which is responsible for~17%of cancers.Comprehensive prevention and control strategies in China should include effective tobacco-control policy,recommendations for healthier lifestyles,along with enlarg-ing the coverage of effective screening,educating,and vaccination programs to better sensitize greater awareness control to the general public. | Rui-Mei Feng Yi-Nan Zong Su-Mei Cao Rui-Hua Xu | 2019 | Cancer Communications2019，39，1： | 675 |
| 4 | Natural herbal medicine Lianhuaqingwen capsule anti-influenza A (H1N1) trial: a randomized, double blind, positive controlled clinical trial显示文摘背景 2009 流行性感冒 A (H1N1 ) 病毒感染与严重复杂并发症的高风险被联系并且比另外的报导季节的流行性感冒在整个 wodd 更快正在传播。试图评估在病人的草药的药 Lianhuaqingwen 囊( LHC )与 16-65 年的 244 个病人的一个总数由实时 RT-PCR 与流行性感冒 A ( H1N1 )病毒感染证实了的流行性感冒 A ( H1N1 ) virus.Methods 感染了的性质的功效和安全的这研究被使随机化到之一二治疗各 122 组织病人。每个组分配了收到 LHC 或 Oseltamivir 五天和观察七天。如果他们有 37.4 并且与至少一下列症状的腋的温度,病人在 36 小时病发作以内被注册:鼻音阻塞,流鼻涕的鼻子,咳嗽,酸的喉咙,疲劳,头疼,肌痛,寒冷并且出汗。主要结束点是 244 个病人,的 illness.Results 的持续时间 240 (98.36 ) 有 21 年完成了的中部的年龄的病人在 10 月 24,2009 日和 2009 年 11 月 23 日之间的学习。对待的 LHC 之间没有重要全面差别, Oseltamivir 在病(LHC 69 小时对 Oseltamivir 85 小时 P 0.05 ) 的中部的持续时间或病毒的流的中部的持续时间对待病人(LHC 103 小时对 Oseltamivir 96 小时, P 0.05 ) 。然而,注意 LHC 显著地包括发烧,咳嗽,酸的喉咙,和疲劳(P 0.05 ) 减少了病和症状的持续时间的严厉是值得的。两学习药很好被容忍。没有药联系了严肃的不利事件与 Oseltamivir 相比发生在 study.Conclusions 期间, LHC 完成了病的持续时间和病毒的流的持续时间的类似的治疗学的有效性减小。因此, LHC 可能是为流行性感冒 A (H1N1 ) 病毒感染的一项其他的治疗学的措施。(临床的试用数字:ChiCTR-TRC-00000589 ) 。 | DUAN Zhong-ping JIA Zhen-hua ZHANG Jian LIU Shuang CHEN Yu LIANG Lian-chun ZHANG Chang-qing ZHANG Zong SUN Yan ZHANG Shu-qin WANG Yong-yan WU Yi-ling | 2011 | Chinese Medical Journal2011，，18： | 55 |
| 5 | 丝虫病传播阈值的研究显示文摘在浙江、河南等11个省、自治区选择经过海群生防治、丝虫和蚊媒种类有代表性的地方建点21个,不采取防治措施,纵向观察3-5年。主要结果:①279例残存微丝蚴血症者大部陆续自然转阴;②微丝蚴率,20个点逐年下降,1个点徘徊在原水平;③12个点的蚊媒已未查见幼丝虫。结果表明,在以人饵蚊帐诱捕法调查,蚊媒叮人率为20-50只/人、夜,和有40%左右居民合理使用蚊帐防蚊的情况下,20个点的微丝蚴率,班氏丝虫病点≤1.71%,马来丝虫病点≤1.55%,残存微丝蚴血症者60μl血平均密度大多在3-10条,只有个别的密度较高,丝虫病的传播处于阈值以下,趋于阻断;1个徘徊于原水平的马来丝虫病点,微丝蚴率为2.31%,有1例微丝蚴血症者密度较高(54条/60μl),处于丝虫病传播临界水平。 | Shi Zong Jun | 1994 | 中国寄生虫学与寄生虫病杂志1994，12，1： | 49 |
| 6 | Research on seismic fluid identification driven by rock physics显示文摘Seismic fluid identification works as an effective approach to characterize the fluid feature and distribution of the reservoir underground with seismic data. Rock physics which builds bridge between the elastic parameters and reservoir parameters sets the foundation of seismic fluid identification, which is also a hot topic on the study of quantitative characterization of oil/gas reservoirs. Study on seismic fluid identification driven by rock physics has proved to be rewarding in recognizing the fluid feature and distributed regularity of the oil/gas reservoirs. This paper summarizes the key scientific problems immersed in seismic fluid identification, and emphatically reviews the main progress of seismic fluid identification driven by rock physics domestic and overseas, as well as discusses the opportunities, challenges and future research direction related to seismic fluid identification. Theoretical study and practical application indicate that we should incorporate rock physics, numerical simulation, seismic data processing and seismic inversion together to enhance the precision of seismic fluid identification. | YIN XingYao ZONG ZhaoYun WU GuoChen | 2015 | Science China Earth Sciences2015，58，2： | 49 |
| 7 | Patterns of antiemetic prophylaxis for chemotherapy-induced nausea and vomiting in China显示文摘Background: Few studies have attempted to evaluate the use of antiemetic therapy for chemotherapyinduced nausea and vomiting(CINV) at a national level in China or to assess how treatment regimens adhere to current guidelines. Methods: We searched the China Health Insurance Research Association(CHIRA) Database to identify patients with cancer who were ≥18 years old and received either moderately or highly emetogenic chemotherapy(MEC and HEC, respectively) between 2008 and 2012. Patients' characteristics as well as usage of specific antiemetic regimens were analyzed using descriptive statistics. Results: Of the 14,548 patients included in the study, 6,477 received HEC while 8,071 were treated with MEC. Approximately 89.9% used antiemetics prophylactically to prevent acute CINV and 71.5% for delayed CINV while 9.0% were prescribed antiemetics as rescue therapy. A significantly lower proportion of patients treated with HEC received prophylactic antiemetic therapy for delayed CINV as compared to those treated with MEC(59.4% vs. 81.3%; P<0.001). The HEC group had a slightly lower proportion of patients using a mixed regimen containing a 5-HT3 antagonist to prevent both acute and delayed CINV than the MEC group(P≤0.012); however, a higher proportion received a mixed regimen containing corticosteroids(P≤0.007). Although more than half of the patients in the HEC group took three antiemetics to prevent acute and delayed CINV, these rates were significantly lower than those of the MEC group(both P<0.001). Finally, analysis of the regimens used revealed that there is over-utilization of drugs within the same class of antiemetic. Conclusions: These findings indicate that more attention is needed for treatment of delayed CINV, in terms of both overall use and the components of a typical treatment regimen. | Xianglong Zong Jie Zhang XinJi Jie Gao Jiafu Ji | 2016 | Chinese Journal of Cancer Research2016，28，2： | 49 |
| 8 | Genome-Wide Analysis of WOX Gene Family in Rice,Sorghum,Maize,Arabidopsis and Poplar显示文摘WUSCHEL-related homeobox (WOX) genes form a large gene family specifically expressed in plants.They are known to play important roles in regulating the development of plant tissues and organs by determining cell fate.Recent available whole genome sequences allow us to do more comprehensive phylogenetic analysis of the WOX genes in plants.In the present study,we identified 11 and 21 WOXs from sorghum (Sorghum bicolor) and maize (Zea mays),respectively.The 72 WOX genes from rice (Oryza sativa),sorghum,maize,Arabidopsis (Arabidopsis thaliana) and poplar (Populus trichocarpa) were grouped into three well supported clades with nine subgroups according to the amino acid sequences of their homodomains.Their phylogenetic relationship was also supported by the observation of the motifs outside the homodomain.We observed the variation of duplication events among the nine sub-groups between monocots and eudicots,for instance,more gene duplication events of WOXs within subgroup A for monocots,while,less for dicots in this subgroup.Furthermore,we observed the conserved intron/exon structural patterns of WOX genes in rice,sorghum and Arabidopsis.In addition,WUS (Wuschel)-box and EAR (the ERF-associated amphiphilic repression)-like motif were observed to be conserved among several WOX subgroups in these five plants.Comparative analysis of expression patterns of WOX genes in rice and Arabidopsis suggest that the WOX genes play conserved and various roles in plants.This work provides insights into the evolution of the WOX gene family and is useful for future research. | Xin Zhang Jie Zong Jianhua Liu Jinyuan Yin Dabing Zhang | 2010 | Journal of Integrative Plant Biology2010，52，11： | 38 |
| 9 | The First Imported Case of Monkeypox in the Mainland of China—Chongqing Municipality,China,September 16,2022显示文摘Monkeypox is a zoonotic viral disease caused by the monkeypox virus(MPXV),and historically,all outbreaks have been linked to Africa;however,monkeypox has been posing an alarming challenge to the world in 2022(1)as approximately 60,000 cases have been reported in more than 100 nations and regions worldwide(2).Currently,many cases of monkeypox were identified in many nonendemic countries outside of Central and West Africa,and human-to-human transmission has occurred frequently,especially among men who have sex with men(MSM)presenting new clinical symptoms similar to syphilis and other sexually transmitted infections(3). | Hua Zhao Wenling Wang Li Zhao Sheng Ye Jingdong Song Roujian Lu Hua Zong Changcheng Wu Wei Huang Baoying Huang Yao Deng Ruhan A Wujuan Xie Li Qi Wenbo Xu Hua Ling Wenjie Tan | 2022 | China CDC weekly2022，4，38： | 35 |
| 10 | The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice显示文摘Although AGAMOUS-LIKE6 (AGL6) MADS-box genes are ancient with wide distributions in gymnosperms and angiosperms, their functions remain poorly understood. Here, we show the biological role of the AGL6-1ike gene, OsMADS6, in specifying floral organ and meristem identities in rice (Oryza sativa L.). OsMADS6 was strongly ex- pressed in the floral meristem at early stages. Subsequently, OsMADS6 transcripts were mainly detectable in paleas, lodicules, carpels and the integument of ovule, as well as in the receptacle. Compared to wild type plants, osmads6 mutants displayed altered palea identity, extra glume-like or mosaic organs, abnormal carpel development and loss of floral meristem determinacy. Strikingly, mutation of a SEPALLATA (SEP)-like gene, OsMADS1 (LHS1), enhanced the defect of osmads6 flowers, and no inner floral organs or glume-like structures were observed in whorls 2 and 3 of osmadsl-z osmads6-1 flowers. Furthermore, the osmadsl-z osmads6-1 double mutants developed severely indetermi- nate floral meristems. Our finding, therefore, suggests that the ancient OsMADS6 gene is able to specify 'floral state' by determining floral organ and meristem identities in monocot crop rice together with OsMADS1. | Haifeng Li Wanqi Liang Ruidong Jia Changsong Yin Jie Zong Hongzhi Kong Dabing Zhang | 2010 | Cell Research2010，20，3： | 33 |
| 11 | Effect of Boschniakia rossica on expression of GSTP,p53 and p21^(ras)proteins in early stage of chemical hepatocarcinogenesis and its anti-inflammatory activities in rats显示文摘AIM To investigate the effect of Boschniakiarossica(BR)extract on expression of GST-P,p53 and p21rasproteins in early stage of chemicalhepatocarcinogenesis in rats and its anti-inflammatory activities.METHODS The expression of tumor marker-placental form glutathione S-transferase(GST-P),p53 and p21rasproteins were investigated byimmunohistochemical techniques and ABCmethod.Anti-inflammatory activities of BR werestudied by xylene and croton oil-induced mouseear edema,carrageenin,histamine and hotscald-induced rat pow edema,adjuvant-inducedrat arthritis and cotton pellet-induced mousegranuloma formation methods.RESULTS The 500 mg/kg of BR-H2O extractfractionated from BR-Methanol extract hadinhibitory effect on the formation of DEN-inducedGST-P-positive foci in rat liver(GST-P stainingwas 78% positive in DEN+AAF group vs 20%positive in DEN+AAF+BR group,P<0.05)andthe expression of mutant p53 and p21rasproteinwas lower than that of hepatic preneoplasticlesions(33% and 22% positive respectively inDEN+AAF group vs negative in DEN+AAF+BRgroup).Both CH2Cl2 and H2O extracts from BRhad anti-inflamatory effect in xylene and crotonoil-induced mouse ear edema(inhibitory rateswere 26%-29% and 35%-59%,respectively). BR-H2O extract exhibited inhibitory effect incarrageenin,histamine and hot scald-inducedhind paw edema and adjuvant-induced arthritis inrats and cotton pellet-induced granulomaformation in mice.CONCLUSION BR extract exhibited inhibitory effect on formation of preneoplastic hepatic foci in early stage of rat chemical hepato-carcinogenesis. Both CH2CI2 and H2O extracts from BR exerted anti-inflammatory effect in rats and mice. | Zong Zhu Yin Hai Ling Jin Xue Zhe Yin Tian Zhu Li Ji Shu Quan Zeng Nan Jin Institute for Cancer Research,Yanbian University College of Medicine,Yanji 133000,Jilin Province,China | 2000 | World Journal of Gastroenterology2000，6，6： | 33 |
| 12 | Carbon Dioxide, Methane, and Nitrous Oxide Emissions from a Rice-Wheat Rotation as Affected by Crop Residue Incorporation and Temperature显示文摘Field measurements were made from June 2001 to May 2002 to evaluate the e?ect of crop residue application and temperature on CO2, CH4, and N2O emissions within an entire rice-wheat rotation season. Rapeseed cake and wheat straw were incorporated into the soil at a rate of 2.25 t hm?2 when the rice crop was transplanted in June 2001. Compared with the control, the incorporation of rapeseed cake enhanced the emissions of CO2, CH4, and N2O in the rice-growing season by 12.3%, 252.3%, and 17.5%, respectively, while no further e?ect was held on the emissions of CO2 and N2O in the following wheat- growing season. The incorporation of wheat straw enhanced the emissions of CO2 and CH4 by 7.1% and 249.6%, respectively, but reduced the N2O emission by 18.8% in the rice-growing season. Signi?cant reductions of 17.8% for the CO2 and of 12.9% for the N2O emission were observed in the following wheat- growing season. A positive correlation existed between the emissions of N2O and CO2 (R2 = 0.445,n = 73,p < 0.001) from the rice-growing season when N2O was emitted. A trade-o? relationship between the emissions of CH4 and N2O was found in the rice-growing season. The CH4 emission was signi?cantly correlated with the CO2 emission for the period from rice transplantation to ?eld drainage, but not for the entire rice-growing season. In addition, air temperature was found to regulate the CO2 emissions from the non-waterlogged period over the entire rice-wheat rotation season and the N2O emissions from the non- waterlogged period of the rice-growing season, which can be quantitatively described by an exponential function. The temperature coe?cient (Q10) was then evaluated to be 2.3±0.2 for the CO2 emission and 3.9±0.4 for the N2O emission, respectively. | ZOU Jianwen(邹建文) HUANG Yao(黄耀) ZONG Lianggang(宗良纲) ZHENG Xunhua(郑循华) WANG Yuesi(王跃思) | 2004 | Advances in Atmospheric Sciences2004，21，5： | 29 |
| 13 | Effectiveness and safety of noninvasive positive-pressure ventilation for severe hypercapnic encephalopathy due to acute exacerbation of chronic obstructive pulmonary disease:a prospective case-control study显示文摘背景尽管严重 encephalopathy 作为可能的禁止徵候被建议了到 noninvasive 的使用积极压力的通风( NPPV ),增加的临床的报告证明它在有损害知觉甚至对尖锐呼吸失败第二等的昏迷的病人是有效的,特别 hypercapnic 尖锐呼吸失败( HARF ) .To 进一步为严重 hypercapnic encephalopathy 评估 NPPV 的有效性和安全。 | ZHU Guang-fa ZHANG Wei ZONG Hua XU Qiu-fen LIANG Ying | 2007 | Chinese Medical Journal2007，，24： | 30 |
| 14 | Clinical impact of plasma TGF-β1 and circulating TGF-β1 mRNA in diagnosis of hepatocellular carcinoma显示文摘BACKGROUND:Transforming growth factor-β(TGF-β) plays an important role in the regulation of cell growth and differentiation,angiogenesis,extracellular matrix formation,immunosuppression and cancer development. In this study,we investigated the levels of TGF-β1 and TGF-β1 mRNA expression,their relationship with HBV replication,and their diagnostic value for hepatocellular carcinoma(HCC). METHODS:Total RNAs were extracted from HCC samples and matched non-tumor tissues,and from peripheral blood mononuclear cells in HCC patients.TGF-β1 mRNA was amplified by RT-PCR and confirmed by DNA sequencing. The distribution of TGF-β1 expression was assessed by immunohistochemistry.The clinical characteristics were analyzed between TGF-β1 and HBV replication.The diagnostic value of circulating TGF-β1 and TGF-β1 mRNA levels were investigated in HCC patients. RESULTS:The incidence of hepatic TGF-β1 expression was 83.3%in HCC samples,43.3%in the surrounding tissues, 94.7%in the HBV DNA-positive group,and 63.6%in the HBV DNA-negative group.Liver TGF-β1 expression was associated with the degree of HCC differentiation and the status of HBV replication,but not with the size or number of tumors.Circulating TGF-β1 level and incidence of TGF-β1 mRNA were significantly higher in the HCC groupthan in any group of patients with benign liver disease, with a higher sensitivity of 89.5%and a specificity of 94.0% for HCC diagnosis when circulating TGF-β1 levels were >1.2μg/L.No significant correlation was found between TGF-β1 expression and AFP level or tumor size.Combining TGF-β1 level and serum AFP raised the detection rate to 97.4%. CONCLUSIONS:Abnormal expression of hepatic TGF-β1 is associated with the degree of HCC differentiation and HBV replication.Both circulating TGF-β1 and TGF-β1 mRNA can be used as sensitive biomarkers for the diagnosis and prognosis of HBV-induced HCC. | Zhi-Zhen Dong,Deng-Fu Yao,Min Yao,Li-Wei Qiu,Lei Zong,Wei Wu, Xin-Hua Wu,Deng-Bing Yao and Xian-Yong Meng Department of Diagnostics,Research Center of Clinical Molecular Biology,Department of Laboratory Science,and Department of GastroenterologyAffiliated Hospital and Institute of Neurosciences,Nantong University,Nantong 226001,China | 2008 | Hepatobiliary & Pancreatic Diseases International2008，7，3： | 27 |
| 15 | The prognostic value of preoperative serum levels of CEA,CA19-9 and CA72-4 in patients with colorectal cancer显示文摘INTRODUCTIONCarcinoembryonic antigen ( CEA) , originally described by Gold and Freedman [1] in 1965, is now an acknowledged member of immunoglobulin superfamily[2],with a role as an intracellular adhesion molecule[3].Carbohydrate antigen 19-9(CA19-9), obtained with a monoclonal antibody produced by immunizing a monoclonal antibody produced by immunizing a mouse with a colonic cancer cell line in 1979[4],is a ligand for E-selectin that plays an important role in the addhesion of cancer cells to endothelial cells [5,6]. | Chao Xu Zheng~1 Wen Hua Zhan~1 Ji Zong Zhao~2 Dong Zheng~3 Dong Ping Wang~1 Yu Long He~1 Zhang Qing Zheng~1 ~1Department of General Surgery,~2Laboratory of Surgery,~3Department of Medicine,First Affiliated Hospital,Sun Yat-Sen University of Medical Sciences,Guangzhou 510080,Guangdong Province,ChinaDr.Chao Xu Zheng now working as a surgeon and lecturer in the Department of General Surgery,First Affiliated Hospital,Sun Yat-Sen University of Medical Sciences,who is a Ph.D.student,having 7 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 25 |
| 16 | Combined serum hepatoma-specific alpha-fetoprotein and circulating alpha-fetoprotein-mRNA in diagnosis of hepatocellular carcinoma显示文摘BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide, its prognosis is poor, and early detection is of utmost importance. Although alpha-fetoprotein (AFP) is a useful marker for detecting and monitoring HCC development, the false-negative or false-positive rates with AFP alone may be as high as 30%-40% for patients with small HCCs. To enhance the specificity and accuracy of AFP measurements for HCC, we analyzed AFP expression states in livers, detected the hepatoma-specific AFP (HS-AFP) fraction and AFP-mRNA from peripheral blood mononuclear cells, and explored their clinical implications for HCC diagnosis. METHODS: AFP expression and hepatocyte distributions in liver specimens were investigated by an immunohistoche- mical assay. Total RNAs were extracted from circulating blood, synthesized to cDNA through random primers and reverse transcriptase, and fragments of the AFP gene were amplified by a nested-PCR assay. The HS-AFP fraction was separated by lectin-affinity chromatography and its level was detected by radioimmunoassay. RESULTS: The incidence of AFP was 73.3% in HCC tissues and its expression in HCCs with moderate or low differentiation was significantly stronger than that of HCCs with high differentiation (P<0.05). The incidence of AFP gene fragments was 100% in HCCs, and 60% in paracancerous tissues (P<0.01). In the HCC and liver cirrhosis groups, the incidence of HS-AFP was 91.7% and 18% (P<0.01), and of AFP-mRNA was 56.7% and 16% (P<0.01), respectively, and neither was found in controls.HS-AFP or AFP-mRNA was not significantly related to size or number of HCC, but to its differentiation, metastasis, and relapse (P<0.05). CONCLUSIONS: Different AFP expression is present in different parts of HCC tissues. HS-AFP and AFP-mRNA fragments improve sensitivity and specificity, and both are useful markers to diagnose HCC or monitor metastasis and relapse. | Wei Wu, Deng-Fu Yao, Yong-Mei Yuan, Ji-Wei Fan, Xiu-Feng Lu, Xiao-Hua Li, Li-Wei Qiu, Lei Zong and Xin-Hua Wu Research Center of Clinical Molecular Biology, Affiliated Hospital of Nantong University, Nantong 226001, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，4： | 26 |
| 17 | Overview of Citrin Deficiency:SLC25A13 Mutations and the Frequency显示文摘Citrin deficiency,autosomal recessive disorder,caused by mutation of SLC25A13 gene on chromosome 7q21.3 has two major phenotypes:neonatal intrahepatic cholestatic hepatitis(NICCD)and adult-onset type Ⅱ citrullinemia(CTLN2).So far,we have identified 52 SLC25A13 mutations and diagnosed the patients not only in Japan(166 CTLN2 and 238 NICCD) but also in other countries.We have detected 76 Chinese,13 Korean and 15 Vietnamese patients with the same mutations as Japanese,and 13 patients(from Israel,UK,USA or Czech)with mutations different from those found in Japanese,indicating a wide distribution of citrin deficiency.DNA diagnoses of 13 known SLC25A13 mutations revealed that the carrier frequency was high in East Asian populations:Chinese(73/4 600=1/63),Japanese(21/1 372=1/65) and Korean(25/2 690=1/108),suggesting that near by 100 000 East Asians are homozygotes.It is important to find out patients with citrin deficiency,to treat them,and to prevent onset of severe CTLN2. | Keiko Kobayashi Miharu Ushtkai Yuan - Zong Song Hong - Zhi Gao Jian - Sheng Sheng Ayako Tabata Furnihiko Okumura Sayaka lkeda Takeyori Saheki | 2008 | 实用儿科临床杂志2008，23，20： | 26 |
| 18 | Clinicopathologic features, diagnosis and surgical treatment of intrahepatic cholangiocarcinoma in 104 patients显示文摘BACKGROUND: The outcome of surgical treatment of pa- tients with intrahepatic cholangiocarcinoma (ICC) is poor. This study was designed to analyze the relationship between clinicopathologic features and the survival time after opera- tion. METHODS: The operation was performed in 104 patients with mass-forming type ICC at our hospital between No- vember 1996 and May 2000. Seventy-nine patients (76.0%) were followed up successfully. Sixteen clinicopathological variables including age, sex, history of chronic liver di- sease , HBsAg, operation, adjuvant therapy, ascites, lymph node metastasis, invasion of adjacent organs, tumor size, necrosis of tumor, envelope, intrahepatic metastasis, Inter- national Union Against Cancer (UICC) TNM staging, his- tology, and cirrhosis were selected for univariate and multi- variate analyses to evaluate their influence on the prognosis. RESULTS: The accumulative 1-, 3-, 5-year survival rates of the 79 patients were 49.4%, 17.3%, 9.6% respectively. Univariate analysis revealed that sex (P=0.0221), HBsAg (P=0.0115), operation (P=0.0042), adjuvant therapy (P= 0.0389), ascites (P=0.0001), invasion (P=0.0220), intra- hepatic metastasis (P=0.0000) and TNM stage (P= 0.0001) were related to survival time. Multivariate analysis revealed that HBsAg, ascites and TNM stage were signifi- cantly related to prognosis. CONCLUSION: Early diagnosis and treatment and major hepatectomy are essential to improving the results of surgi- cal treatment of ICC patients. | Xiao-Hui Fu, Zhao-Hui Tang, Ming Zong, Guang-Shun Yang, Xiao-Ping Yao and Meng-Chao Wu Shanghai, China Department of Comprehensive Therapy of Hepatobi- liary Tumors, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，2： | 24 |
| 19 | Protective effects of polydatin against CCl_4-induced injury to primarily cultured rat hepatocytes显示文摘NTRODUCTIONPolygonumcuspidatumSieb.etZucc.(Polygonaceae)isatraditionalChineseherbaldrug,withbitertasteandcoldnature.Itmainly... | HUANG Zhao Sheng, WANG Zong Wei, LIU Ming Ping, ZHONG Shi Qing, LI Qiao Mei and RONG Xiang Lu | 1999 | World Journal of Gastroenterology1999，5，1： | 24 |
| 20 | On the Hyper-Order of Solutions of Some Second-Order Linear Differential Equations | CHEN Zong Xuan Department of Mathematics. Jiangxi Normal University. Nanchang 330027. P. R. China | 2002 | Acta Mathematica Sinica,English Series2002，18，1： | 24 |
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