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您的检索式：作者名="Yushan Zou"
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| 1 | Fabrication of Sulfated Silk Fibroin‑Based Blend Nanofbrous Membranes for Lysozyme Adsorption显示文摘In this work,the silk fbroin/cellulose blend nanofbrous membranes modifed with sodium-3-sulfobenzoate(S-SCBNM)were fabricated by electrospinning technique for lysozyme adsorption.The morphology and structure of membranes was observed.The efect of sulfate contents,pH values and concentration of lysozyme on adsorption performance were studied,respectively.The reuse capacity was evaluated.The results showed that the resultant S-SCBNM exhibited integrated properties of ultrathin fber diameter(148 nm),fast adsorption equilibrium,excellent adsorption performance(636 mg g−1)and good reversibility.We envision that this new class of green and natural blend membranes is particularly promising for the development of proteins separation. | Ying Zhou Jiaxue Wu Yanyi Li Wei Zhang Yushan Zou Lian Duan Xiao Yang Bo Xiao Shixiong Yi | 2022 | Advanced Fiber Materials2022，4，1： | 1 |
| 2 | On Network Buzzwords From Meme Theory-“Mind”显示文摘In the era of network media,catchwords have become a discourse fact and cultural phenomenon with great influence in China in recent years.Language is in the sensitive dynamic change,common words develop into catchwords,and in addition to its own development,more important is often from the external cause of social events.This paper takes network buzzwords as the entry point to analyze the“Mind”of buzzwords.Although the word has always appeared in the public’s vision,it has gained a lot of attention after developing into buzzwords driven by hot social events.This paper explains the reasons for its popularity with the help of relevant theories of memetics,aiming to prove the inevitability of“Mind”of buzzwords becoming a powerful language on the Internet. | ZOU Yushan Ni Rong | 2022 | US-China Foreign Language2022，20，8： | 0 |
| 3 | A genetically engineered Escherichia coli that senses and degrades tetracycline antibiotic residue显示文摘Due to the abuse of antibiotics,antibiotic residues can be detected in both natural environment and various industrial products,posing threat to the environment and human health.Here we describe the design and implementation of an engineered Escherichia coli capable of degrading tetracycline(Tc)-one of the commonly used antibiotics once on humans and now on poultry,cattle and fisheries.A Tcdegrading enzyme,TetX,from the obligate anaerobe Bacteroides fragilis was cloned and recombinantly expressed in E.coli and fully characterized,including its Km and kcat value.We quantitatively evaluated its activity both in vitro and in vivo by UVeVis spectrometer and LC-MS.Moreover,we used a tetracycline inducible amplification circuit including T7 RNA polymerase and its specific promoter PT7 to enhance the expression level of TetX,and studied the dose-response of TetX under different inducer concentrations.Since the deployment of genetically modified organisms(GMOs)outside laboratory brings about safety concerns,it is necessary to explore the possibility of integrating a kill-switch.Toxin-Antitoxin(TA)systems were used to construct a mutually dependent host-plasmid platform and biocontainment systems in various academic and industrious situations.We selected nine TA systems from various bacteria strains and measured the toxicity of toxins(T)and the detoxifying activity of cognate antitoxins(A)to validate their potential to be used to build a kill-switch.These results prove the possibility of using engineered microorganisms to tackle antibiotic residues in environment efficiently and safely. | Zepeng Mu Zhuoning Zou Ye Yang Wenbo Wang Yue Xu Jianyi Huang Ruiling Cai Ye Liu Yajin Mo Boyi Wang Yiqun Dang Yongming Li Yushan Liu Yueren Jiang Qingyang Tan Xiaohong Liu Cheng Hu Hua Li Sha Wei Chunbo Lou Yang Yu Jiangyun Wang | 2018 | Synthetic and Systems Biotechnology2018，3，3： | 0 |
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