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您的检索式：作者名="Yingchen Ling"
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| 1 | Regulation of shear stress on rolling behaviors of HL-60 cells on P-selectin显示文摘Circulating leukocytes in trafficking to the inflammatory sites, will be first tether to, and then roll on the vascular surface. This event is mediated through specific interaction of P-selectin and P-selectin glycoprotein ligand-1(PSGL-1), and regulated by hemodynamics. Poor data were reported in understanding P-selectin-mediated rolling. With the flow chamber technique, we herein observed HL-60 cell rolling on P-selectin with or without 3% Ficoll at various wall shear stresses from 0.05 to 0.4 dyn/cm2. The results demonstrated that force rather than transport regulated the rolling, similar to rolling on L- and E-selectin. The rolling was accelerated quickly by an increasing force below the optimal shear threshold of 0.15 dyn/cm2 first and then followed by a slowly decelerating phase starting at the optimum, showing a catch-slip transition and serving as a mechanism for the rolling. The catch-slip transition was completely reflected to the tether lifetime and other rolling parameters, such as the mean and fractional stop time. The narrow catch bond regime stabilized the rolling quickly, through steeply increasing fractional stop time to a plateau of about 0.85. Data presented here suggest that the low shear stress threshold serves as a mechanism for most cell rolling events through P-selectin. | LING YingChen FANG Ying YANG XiaoFang LI QuHuan LIN QinYong WU JianHua | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，10： | 4 |
| 2 | Force-dependent calcium signaling and its pathway of human neutrophils on P-selectin in flow显示文摘P-selectin glycoprotein ligand-1 (PSGL-1 ) 的 P-selectin 约会引起传播白血球滚动在上并且遵守脉管的表面,并且调停细胞内部的钙流动,为在坚定地的随后的逮捕并且移居进感染的一个关键却不清楚的事件或受伤织物。用一种平行的板流动房间技术和细胞内部的钙离子察觉者(Fluo-4 AM ) ,当在各种各样的墙的 chemokines 不在时的使不能调动的 P-selectin 上的坚定地粘住的 neutrophils 的细胞内部的钙流动砍压力被荧光显微镜学在实时这里调查。结果示威了,力量被触发,提高并且加快并且,使不能调动的 P-selectin 上的 neutrophils 爆炸的细胞质的钙 PSGL-1 的那个 P-selectin 约会在流动导致了坚定地粘住的 neutrophils 的细胞内部的钙流动,增加集中提高了细胞的钙发信号的 P-selectin。发信号的这 P-selectin-induced 钙应该来自细胞内部的钙版本而非细胞外的钙流入,并且沿着包含细胞骨架, moesin 和怒气酷氨酸 kinase (Syk ) 的 mechano 化学的信号小径。这些结果为 P-selectin-induced 钙在流动发信号 neutrophils 提供新奇卓见进 mechano 化学的规定机制。 | Bing Huang Yingchen Ling Jiangguo Lin Xin Du Ying Fang Jianhua Wu | 2017 | Protein & Cell2017，8，2： | 3 |
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