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| 1 | Essential roles of exosome and circRNA_101093 on ferroptosis desensitization in lung adenocarcinoma显示文摘Background:Resistance to ferroptosis,a regulated cell death caused by irondependent excessive accumulation of lipid peroxides,has recently been linked to lung adenocarcinoma(LUAD).Intracellular antioxidant systems are required for protection against ferroptosis.The purpose of the present studywas to investigate whether and how extracellular system desensitizes LUAD cells to ferroptosis.Methods:Established human lung fibroblasts MRC-5,WI38,and human LUAD H1650,PC9,H1975,H358,A549,and H1299 cell lines,tumor and matched normal adjacent tissues of LUAD,and plasma from healthy individuals and LUAD patients were used in this study.Immunohistochemistry and immunoblotting were used to analyze protein expression,and quantitative reverse transcription-PCR was used to analyze mRNA expression.Cell viability,cell death,and the lipid reactive oxygen species generationwere measured to evaluate the responses to ferroptosis.Exosomes were observed using transmission electron microscope.The localization of arachidonic acid(AA)was detected using click chemistry labeling followed by confocal microscopy.Interactions between RNAs and proteins were detected using RNA pull-down,RNA immunoprecipitation and photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation methods.Proteomic analysis was used to investigate RNA-regulated proteins,and metabolomic analysis was performed to analyze metabolites.Cellderived xenograft,patient-derived xenograft,cell-implanted intrapulmonary LUAD mouse models and plasma/tissue specimens from LUAD patients were used to validate the molecular mechanism.Results:Plasma exosome from LUAD patients specifically reduced lipid peroxidation and desensitized LUAD cells to ferroptosis.A potential explanation is that exosomal circRNA_101093(cir93)maintained an elevation in intracellular cir93 in LUAD to modulate AA,a poly-unsaturated fatty acid critical for ferroptosisassociated increased peroxidation in the plasma membrane.Mechanistically,cir93 interacted with and increased fatty acid-binding protein 3(FABP3),which transported AA and facilitated its reaction with taurine.Thus,global AA was reduced,whereas N-arachidonoyl taurine(NAT,the product of AA and taurine)was induced.Notably,the role of NAT in suppressing AA incorporation into the plasma membrane was also revealed.In pre-clinical in vivo models,reducing exosome improved ferroptosis-based treatment.Conclusion:Exosome and cir93 are essential for desensitizing LUAD cells to ferroptosis,and blocking exosome may be helpful for future LUAD treatment. | Xiao Zhang Yunhua Xu Lifang Ma Keke Yu Yongjie Niu Xin Xu Yi Shi Susu Guo Xiangfei Xue Yikun Wang Shiyu Qiu Jiangtao Cui Hong Wang Xiaoting Tian Yayou Miao Fanyu Meng Yongxia Qiao Yongchun Yu Jiayi Wang | 2022 | Cancer Communications2022，42，4： | 10 |
| 2 | The evolution of the Kuroshio Current over the last 5 million years since the Pliocene: Evidence from planktonic foraminiferal faunas显示文摘Meridional heat transport of the western Pacific boundary current(the Kuroshio Current)is one of the key factors in global climate change.This current is important because it controls the temperature gradient between low latitudes and the North Pacific and so significantly influences mid-latitude atmosphere-ocean interactions.Here we reconstruct changes in hydrological conditions within the mid-latitude mainstream of the Kuroshio Current based on faunal analysis of planktonic foraminifera in core DSDP 296 from the Northwest Pacific Ocean.This approach enabled us to deduce evolutionary processes within the Kuroshio Current since the Pliocene.A total of 57 species in the coarser section(>150μm)were identified;results indicate that planktonic foraminiferal faunal evolution has mainly been characterized by three major stages,the first of which comprised mixed-layer warm-water species of Globigerinoides ruber which first appeared between 3.5 and 2.7 Ma and then gradually increased in content.Percentages of another warm-water species of G.conglobatus also gradually increased in number over this interval.Variations in warm-water species indicate a gradual rise in sea surface temperature(SST)and imply initiation of Kuroshio Current impact on the Northwest Pacific Ocean since at least 3.5 Ma.Secondly,over the period between 2.7 and 2.0 Ma,thermocline species of Globigerina calida,Neogloboquadrina humersa,Neogloboquadrina dutertrei,and Pulleniatina obliquiloculata started to appear in the section.This fauna was dominated by G.ruber as well as increasing G.conglobatus contents.These features imply a further rise in SST and its gradually enhanced influence on thermocline water,suggesting strengthening of the Kuroshio Current since 2.7 Ma.Thirdly,between 2.0 Ma and present,increasing contents of thermocline species(i.e.,G.calida,N.dutertrei and P.obliquiloculata)indicate a gradual rise in seawater temperature at this depth and also imply more intensive Kuroshio Current during this period.On the basis of comparative records from cores ODP 806 and DSDP292 from the low latitude Western Pacific,we propose that initiation of the impact of the Kuroshio Current in the Northwest Pacific and it subsequent stepwise intensifications since 3.5 Ma can be closely related to the closure and restriction of the Indonesian and Central American seaways as well as variations in the Western Pacific Warm Pool(WPWP)and equatorial Pacific region. | Jia WANG Fengming CHANG Tiegang LI Hanjie SUN Yikun CUI Tianhao LIU | 2020 | Science China Earth Sciences2020，63，11： | 1 |
| 3 | A novel ant colony system based on delauney triangu- lation and self-adaptive mutation for TSP显示文摘 | WANG Chaoxue CUI Duwu ZHANG Yikun | 2006 | Interna- tional Journal of Information Technology2006，12，3： | 1 |
| 4 | A novel ant colony system based on cure mechanism of traditional chinese medicine for TSP显示文摘 | Wang Chaoxue Cui Duwu Zhang Yikun | 2006 | International Journal of Computer Science and Network Security2006，6，5： | 1 |
| 5 | Sea surface temperature evolution in the Yellow Sea Warm Current pathway and its teleconnection with high and low latitude forcing during the mid-late Holocene显示文摘Sea surface temperature(SST)in the Yellow Sea Warm Current(YSWC)pathway is sensitive to the East Asian Winter Monsoon(EAWM)and YSWC.However,the role of the YSWC in the evolution of regional SST remains unclear.Here,we present new U 37 k′based SST and grain size sequences spanning the last 6092 years in the sediment core Z1,which was retrieved from the central Yellow Sea muddy area.Overall,U 37 k′-SST gradually increased since 6.1 ka BP,with a series of centennial-scale fl uctuations.Its variation was mainly caused by EAWM when YSWC was weak between 6.1 and~3.9 ka BP,as shown by the end-member content of grain size.However,after YSWC was fully developed,i.e.,since~3.9 ka BP,it exerted critical eff ects on SST evolution in its pathway.The 1010-and 538-year cycles of the SST sequence indicated a basic control of solar activity on the oceanic conditions in the Yellow Sea.It is suggested that the variation of total solar irradiance was amplifi ed by thermohaline circulation and then transmitted to the Yellow Sea through the EAWM.Meanwhile,the tropical Pacifi c signal of El Niño was transmitted to the YSWC through the Kuroshio Current.The dual properties of warm water transported by YSWC to compensate the EAWM and driving by Kuroshio Current closely linked the variation of SST in the YSWC pathway to the Northern Hemisphere high latitude climate and the tropical Pacifi c.These fi ndings highlight the signifi cance of YSWC on regional SST evolution and its teleconnection to high and low latitude forcing,which grains a better understanding of the long-term evolution of SST in the middle latitude Yellow Sea. | Zhong PI Fengming CHANG Tiegang LI Yikun CUI | 2022 | Journal of Oceanology and Limnology2022，40，1： | 1 |
| 6 | Decoupling Algorithms for the Gravitational Wave Spacecraft显示文摘The gravitational wave spacecraft is a complex multi-input multi-output dynamic system.The gravitational wave detection mission requires the spacecraft to achieve single spacecraft with two laser links and high-precision control.Establishing one spacecraftwith two laser links,compared to one spacecraft with a single laser link,requires an upgraded decoupling algorithmfor the link establishment.The decoupling algorithmwe designed reassigns the degrees of freedomand forces in the control loop to ensure sufficient degrees of freedomfor optical axis control.In addressing the distinct dynamic characteristics of different degrees of freedom,a transfer function compensation method is used in the decoupling process to further minimize motion coupling.The open-loop frequency response of the systemis obtained through simulation.The upgraded decoupling algorithms effectively reduce the open-loop frequency response by 30 dB.The transfer function compensation method efficiently suppresses the coupling of low-frequency noise. | XueWang Weizhou Zhu Zhao Cui Xingguang Qian Jinke Yang Jianjun Jia Yikun Wang | 2024 | Computer Modeling in Engineering & Sciences2024，140，7： | 0 |
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