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| 1 | A candidate DNA vaccine elicits HCV specific humoral and cellular immune responses显示文摘AIM: TO investigate the immunogenicity of candidate DNA vaccine against hepatitis C virus (HCV) delivered by two plasmids expressing HCV envelope protein 1 (El) and envelope protein 2 (E2) antigens respectively and to study the effect of CpG adjuvant on this candidate vaccine.METHODS: Recombinant plasmJds expressing HCV EI and E2 antigens respectively were used to simultaneously inoculate mice with or without CpG adjuvant. Antisera were then collected and tJters of antJ-HCV antibodies were analyzed by ELISA. One month after the last injection, animals were sacrificed to prepare single-cell suspension of splenocytes.These cells were subjected to HCV antigen specific proliferaion assays and cytokine secretion assays to evaluate the cellular immune responses of the vaccinated animals.RESULTS: Antibody responses to HCV EI and E2 antigens were detected in vaccinated animals. Animals receiving CpG adjuvant had slightly lower titers of anti-HCV antibodies in the sera, while the splenocytes from these animals showed higher HCV-antigen specific proliferation. Analysis of cytokine secretion from the splenocytes was consistent with the above results. While no antigen-specific IL-4 secretion was detected for all vaccinated animals, HCV antigen-specific INF-γ, secretion was detected for the splenocytes of vaccinated animals. CpG adjuvant enhanced the secretion of INF-γ, but did not change the profile of IL-4 secretion.CONCLUSION: Vaccination of mice with plasmids encoding HCV E1 and E2 antigens induces humoral and cellular immune responses. CpG adjuvant significantly enhances the cellular immune response. | Li-XinZhu JingLiu YeYe You-HuaXie Yu-YingKong Guang-DiLi YuanWang | 2004 | World Journal of Gastroenterology2004，10，17： | 12 |
| 2 | Expression of hepatitis C virus envelope protein 2 induces apoptosis in cultured mammalian cells显示文摘AIM: To explore the role of hepatitis C virus (HCV) envelope protein 2 (E2) in the induction of apoptosis. METHODS: A carboxyterminal truncated E2 (E2-661) was transiently expressed in several cultured mammalian cell lines or stably expressed in Chinese hamster ovary (CliO) cell line. Cell proliferation was assessed by ^3H thymidine uptake. Apoptosis was examined by Hoechst 33258 staining, flow cytometry and DNA fragmentation analysis. RESULTS: Reduced proliferation was readily observed in the E2-661 expressing cells. These cells manifested the typical features of apoptosis, including cell shrinkage, chromatin condensation and hypodiploid genomic DNA content. Similar apoptotic cell death was observed in an E2-661 stably expressing cell line. CONCLUSION: HCV E2 can induce apoptosis in cultured mammalian cells. | Li-XinZhu JingLiu You-HuaXie Yu-YingKong YeYe Chun-LinWang Guang-DiLi YuanWang | 2004 | World Journal of Gastroenterology2004，10，20： | 6 |
| 3 | Study on the Detection Methods of DC Series Arc Fault显示文摘It is difficult to detect and extinguish direct current(DC)arc in power electronics systems,and the arc could easily lead to a fire and cause great damage to surrounding equipment.A DC arc generation simulation unit is established,in which DC series arcs are generated by dragging the moving electrode away from the fixed one with the help of the stepper motor.In addition,a ferrite rod antenna is used to receive the electromagnetic radiation signals induced by the arcs.Based on experiments using the unit,the general characteristics of DC arc,including the pulse characteristics of arc current and source output in corresponding time window,and the frequency-domain characteristics of arc current,are studied.With discussion on three detection methods,it is concluded that the variation of current and voltage of arc,the spectrum of the arc current during the discontinuous intervals and the radiating electromagnetic signal are all features that can be adopted for detecting DC series arc.Therefore,a synthetic judgment method is suggested for further study. | JI Shengchang LIU Yuan ZHU Yeye ZHU Lingyu | 2013 | 高电压技术2013，39，9： | 3 |
| 4 | CRISPR-Cas9: A method for establishing rat models of drug metabolism and pharmacokinetics显示文摘The 2020 Nobel Prize in Chemistry recognized CRISPR-Cas9,a super-selective and precise gene editing tool.CRISPR-Cas9 has an obvious advantage in editing multiple genes in the same cell,and presents great potential in disease treatment and animal model construction.In recent years,CRISPRCas9 has been used to establish a series of rat models of drug metabolism and pharmacokinetics(DMPK),such as Cyp,Abcb1,Oatp1 b2 gene knockout rats.These new rat models are not only widely used in the study of drug metabolism,chemical toxicity,and carcinogenicity,but also promote the study of DMPK related mechanism,and further strengthen the relationship between drug metabolism and pharmacology/toxicology.This review systematically introduces the advantages and disadvantages of CRISPR-Cas9,summarizes the methods of establishing DMPK rat models,discusses the main challenges in this field,and proposes strategies to overcome these problems. | Jian Lu Jie Liu Yuanqing Guo Yuanjin Zhang Yeye Xu Xin Wang | 2021 | Acta Pharmaceutica Sinica B2021，11，10： | 2 |
| 5 | An integrated remote sensing and GIS approach in the monitoring and evaluation of rapid urban growth for sustainable development in the Pearl River Delta, China 显示文摘 | Yey A G O Li X | 1997 | International Planning Studies1997，2，2： | 1 |
| 6 | Genome manipulationincarp (Cyprinus carpio) 显示文摘 | WuC YeY ChenR | 1986 | Aquaculture1986，54，： | 1 |
| 7 | Keyword + + : A Frame- work to Improve Keyword Search over Entity Data- bases显示文摘 | Ganti V He Yeye Xin Dong | 2010 | Proceedings of the VLDB Endowment2010，3，12： | 1 |
| 8 | The synthesis of dialkoxyphosphorylcarboxamides 显示文摘 | YEY LIP HUXL | 2008 | Phosphorus Sulfur and Silicon and the Related Elements2008，，： | 1 |
| 9 | Separation and Recovery of Chromium and Vanadium from Vana- dium-Containing Chromate Solution by Ion Exchange 显示文摘 | Fan Yeye Wang Xuewen Wang Mingyu | 2013 | Hydrometallurgy2013，136，： | 1 |
| 10 | Recovery of vanadium from stone coal acid leaching solution by coprecipitation, alkaline roasting and water leaching显示文摘 | Puhong Ye Xuewen Wang Mingyu Wang Yeye Fan Xiaoyan Xiang | 2012 | Hydrometallurgy2012，，： | 1 |
| 11 | StudiesonchemicalconstituentsfromPoriacocos(Schw)Wolf(茯苓化学成分研究)显示文摘 | HUB(胡斌) YANGYP(杨益平) YEY(叶阳) | 2010 | ChineseTraditionalandHerbalDrugs2010，05，： | 1 |
| 12 | An Integrated Remote Sensing and GIS Approach in the Monitoring and Evaluation of Rapid Urban Growth for Sustainable Development in the Pearl River Delta China显示文摘 | Li X | 1997 | International Planning Studies1997，2，2： | 1 |
| 13 | Separation and recovery of chromium and vanadium from vanadium-containing chromate solution by ion exchange显示文摘 | Yeye Fan Xuewen Wang Mingyu Wang | 2013 | Hydrometallurgy2013，，： | 1 |
| 14 | 查看详情显示文摘 | XIAO J YEY YOU H | | 0，，： | 1 |
| 15 | An evaluation of the bonding of glass-ionomer restoratives to dentine and enamel 显示文摘 | Jenkins CBG | 1986 | Br Dent J1986，161，5： | 1 |
| 16 | Early to intermediate steps of tumor embolic formation involve specific proteolytic processing of E-cadherin regulated by Rab7显示文摘 | YeY Gao lX TianH etal | 2012 | Mol Cancer Res2012，10，6： | 1 |
| 17 | An Integrated Remote Sensing and GIS Approach in The Monitoring and Evaluation of Rapid Urban Growth for Sustainable Development in The Pearl River Delta, China显示文摘 | Li X | 1997 | International Planning Studies1997，2，2： | 1 |
| 18 | Copper(Ⅱ)-catalyzed synthesis of N-substituted-3-amino-4-cyanoisoquinoline-1(2H)-ones by the reaction of N-substituted-2-iodobenzamides with malononitrile显示文摘 | LIU Yeye ZENG Runsheng PAN Jie | 2014 | Chin J Chem2014，32，9： | 1 |
| 19 | A fiilly polynomial-time approximation algorithm forcomputing a stationary point of the general linearcomplementarity problem 显示文摘 | YeY | 1993 | Mathematics of OpreationsResearch1993，18，2： | 1 |
| 20 | Crawling Deep Web Entity Pages显示文摘 | Yeye He Dong Xin Venkatesh Ganti | 2013 | WSDM2013，，4： | 1 |
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