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| 1 | Protein adsorption on the poly(L-lactic acid) surface modified by chitosan and its derivatives显示文摘Surface modification of biomaterials has been adopted over the years to improve their biocompatibility. In this study, aiming to promote hydrophilicity and to introduce natural recognition sites onto poly(L-lactic acid) (PLLA) films, chitosan and its derivatives, carboxymethyl chitosan(CMC) and N-methylene phosphonic chitosan (NPC), were used to modify the surface of PLLA films by an entrapment method. Radiolabeled (125I) proteins were used to measure the amount of protein adsorbed to PLLA surfaces. Fibronectin (Fn) was used to study the protein adsorption on the modified PLLA surfaces, including isotherm adsorption and adsorption kinetics of single protein, competitive adsorption of binary proteins system and serum multi-proteins and the desorption behavior in serum solution. The results showed that in the isotherm adsorption, Fn had a larger adsorption capacity on the CS-modified surface at lower concentrations, but had a high adsorption capacity at CMC-modified surface at higher concentrations. In the study of absorption kinetics, Fn had a fastest adsorption equilibrium and a highest equilibrium adsorption capacity at the CS-modified surface, while it was opposite at the PCS-modified surface. When BSA and serum were added, it had the greatest effect on the adsorption of Fn on the PCS-modified surface. After 6 hours soaking in the desorption study, Fn had reached desorption equilibrium on all the modified surfaces, which had different effects on the desorption rate and the remaining percentage of Fn. | JIAO YanPeng ZHOU ChangRen LI LiHua DING Shan LU Lu LUO BingHong LI Hong | 2009 | Chinese Science Bulletin2009，54，18： | 2 |
| 2 | Study on effect of underground heat capacity on dynamic simulation of transient flow in natural gas pipelines 显示文摘 | DING Yanpeng LI Yuxing | 2011 | Journal of China university of petroleum China (Edition of natural science)2011，3，： | 1 |
| 3 | New paleomagnetic results from Ceno-Mesozoic volcanic rocks along southern rim of the Tarim Basin, China*显示文摘 | Meng Zifang Deng Yunshan Ding Zhaohai Zheng Yanpeng Li Yong’an Sun Dongjiang | 1998 | Science in China Series D: Earth Sciences1998，，2： | 1 |
| 4 | Study on the structure of heteropolymer pp38/pp24 and its enhancement on the bi-directional promoter upstream of pp38 gene in Marek’s disease virus显示文摘In the latest report, Chloramphenicol acetyltransferase (CAT) gene was used as a reporter to investi-gate the influence of pp38 on its upstream bi-directional promoter, and it was found that the co-expression of pp38 and pp24 can significantly enhance the transactivity of the bi-directional pro-moter between pp38 gene and 1.8-kb mRNA transcript in genome of Marek’s disease virus (MDV). In this study, enhanced green fluorescence protein (EGFP) gene was used as another reporter to further investigate the promoter activity. The transfection shows the promoter has the complete activity under the condition of co-expression of pp38 and pp24 in the same cells. Immunoprecipitation test was used to verify the structure of pp38/pp24 heteropolymer. The pp38-specific monoclonal antibody H19 was used in this test, and pp38, pp24 or both were prepared from the pcDNA-pp38, pcDNA-pp24 or pBud-pp38-pp24 transfected chicken embryonic fibroblast (CEF), respectively. Immunoprecipitation indicates that pp24 could be co-precipitated with pp38 by MabH19, implying that pp24 and pp38 were able to form a heteropolymer in the natural condition. The two separated tests clarify that pp38 and pp24 form a heteropolymer, which enhances the activity of the promoter. | DING JiaBo CUI ZhiZhong JIANG ShiJin LI YanPeng | 2008 | Science China(Life Sciences)2008，51，9： | 1 |
| 5 | TaACTIN7-D regulates plant height and grain shape in bread wheat显示文摘Exploitation of new gene resources and genetic networks contributing to the control of crop yield-related traits,such as plant height,grain size,and shape,may enable us to breed modern high-yielding wheat varieties through molecular methods.In this study,via ethylmethanesulfonate mutagenesis,we identify a wheat mutant plant,mu-597,that shows semi-dwarf plant architecture and round grain shape.Through bulked segregant RNA-seq and map-based cloning,the causal gene for the semi-dwarf phenotype of mu-597 is located.We find that a single-base mutation in the coding region of TaACTIN7-D(TaACT7-D),leading to a Gly-to-Ser(G65S)amino acid mutation at the 65th residue of the deduced TaACT7-D protein,can explain the semi-dwarfism and round grain shape of mu-597.Further evidence shows that the G65S mutation in TaACT7-D hinders the polymerization of actin from monomeric(G-actin)to filamentous(F-actin)status while attenuates wheat responses to multiple phytohormones,including brassinosteroids,auxin,and gibberellin.Together,these findings not only define a new semi-dwarfing gene resource that can be potentially used to design plant height and grain shape of bread wheat but also establish a direct link between actin structure modulation and phytohormone signal transduction. | Xiongtao Li Beilu Cao Dejie Du Long Song Lulu Tian Xiaoming Xie Zhaoyan Chen Yanpeng Ding Xuejiao Cheng Yingyin Yao Weilong Guo Zhenqi Su Qixin Sun Zhongfu Ni Lingling Chai Jie Liu | 2023 | Journal of Genetics and Genomics2023，50，11： | 0 |
| 6 | GhSCL4 Acts as a Positive Regulator in Both Transgenic Arabidopsis and Cotton during Salt Stress显示文摘GRAS transcription factors play important roles in plant abiotic stress response,but their characteristics and functions in cotton have not been fully investigated.A cotton SCL4/7 subgroup gene in the GRAS family,GhSCL4,was found to be induced by NaCl treatments.Nuclear localization and transactivation activity of GhSCL4 indicate its potential role in transcriptional regulation.Transgenic Arabidopsis thaliana over-expressing GhSCL4 showed enhanced resistance to salt and osmotic stress.What’s more,the transcript levels of salt stress-induced genes(AtNHX1 and AtSOS1)and oxidation-related genes(AtAPX3 and AtCSD2)were more highly induced in the GhSCL4 over-expression lines than in wild type after salt treatment.Furthermore,silencing of GhSCL4 resulted in reduced salt tolerance in cotton caused by reactive oxygen species(ROS)enrichment under salt treatment,and antioxidant enzyme activities were accordingly significantly reduced in GhSLC4-silenced lines.These results indicated that GhSCL4 enhances salt tolerance of cotton by detoxifying ROS.In addition,the transient expression assay confirmed an interactive relationship between GhSCL4 and GhCaM7,which indicated that salt tolerance conferred by GhSCL4 might be associated with salt-induced Ca^(2+)/CaM7-mediated signaling.Taken together,GhSCL4 acts as a positive regulator in cotton during salt stress that is potentially useful for engineering salt-tolerant cotton. | Yanyan Zhao Yanpeng Ding Bailin Duan Qingzhou Xie | 2023 | Phyton-International Journal of Experimental Botany2023，92，1： | 0 |
| 7 | Supramolecular assembly of Cp1-11 peptide and insulin for rapid-acting formulation显示文摘In order to improve the life quality of diabetic patients,it is very important to develop rapid-acting insulin formulations that can mimic the physiological meal-time secretion profile of insulin in healthy people.Although several insulin analogues have been designed to provide postprandial glycemic control,still there are some serious disadvantages.A supramolecular strategy is presented here to inhibit insulin aggregation and improve its bioactivity by using Cp1-11 peptide.As a fragment of C-peptide in proinsulin,Cp1-11 peptide was found to influence insulin oligomerization by supramolecular interactions.This work demonstrates that the Cp1-11 peptide can interact with oligomeric insulin and facilitate its disaggregation into the physiologically active monomeric form.Computer simulation indicates that Cp1-11 can insert into the space between the C-terminal tail and the N-terminal helix of the B-chain of insulin,causing dissociation of the insulin dimer.The supramolecular assembly of Cp1-11 and insulin can improve the bioavailability and therapeutic effect of insulin on the control of in vivo blood glucose levels.These results suggest that Cp1-11 peptide can modulate the intermolecular interaction of aggregated insulin and prevent the transition from monomeric to multimeric states,and shows great potential for the development of an effective rapid-acting strategy to treat diabetes. | Weigang Wang Sheyu Li Zhouxiang Zhao Anna Zhou Yanpeng Liu Yantao Chen Mingchang Lin Guosong Chen Chunmei Ding Jianshu Li | 2017 | Journal of Bioresources and Bioproducts2017，2，3： | 0 |
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