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| 1 | Nanotechnology-based combination therapy for overcoming multidrug-resistant cancer显示文摘Multidrug resistance(MDR) is a major obstacle to successful cancer treatment and is crucial to cancer metastasis and relapse.Combination therapy is an effective strategy for overcoming MDR. However, the different pharmacokinetic(PK) profiles of combined drugs often undermine the combination effect in vivo, especially when greatly different physicochemical properties(e.g.,those of macromolecules and small drugs) combine. To address this issue, nanotechnology-based codelivery techniques have been actively explored. They possess great advantages for tumor targeting, controlled drug release, and identical drug PK profiles. Thus,a powerful tool for combination therapy is provided, and the translation from in vitro to in vivo is facilitated. In this review, we present a summary of various combination strategies for overcoming MDR and the nanotechnology-based combination therapy. | Meng Zhang Ergang Liu Yanna Cui Yongzhuo Huang | 2017 | Cancer Biology & Medicine2017，14，3： | 10 |
| 2 | Tat-functionalized Ag-Fe_3O_4 nano-composites as tissue-penetrating vehicles for tumor magnetic targeting and drug delivery显示文摘In this paper, we prepared a dual functional system based on dextrin-coated silver nanoparticles which were further attached with iron oxide nanoparticles and cell penetrating peptide(Tat), producing Tat-modified Ag-Fe_3O_4 nanocomposites(Tat-FeAgNPs). To load drugs, an –SH containing linker, 3-mercaptopropanohydrazide, was designed and synthesized. It enabled the silver carriers to load and release doxorubicin(Dox) in a pH-sensitive pattern. The delivery efficiency of this system was assessed in vitro using MCF-7 cells, and in vivo using null BalB/c mice bearing MCF-7 xenograft tumors. Our results demonstrated that both Tat and externally applied magnetic field could promote cellular uptake and consequently the cytotoxicity of doxorubicin-loaded nanoparticles,with the IC_(50) of Tat-FeAgNP-Dox to be 0.63 mmol/L. The in vivo delivery efficiency of Tat-FeAgNP carrying Cy5 to the mouse tumor was analyzed using the in vivo optical imaging tests, in which TatFeAgNP-Cy5 yielded the most efficient accumulation in the tumor(6.772.4% ID of Tat-FeAgNPs).Anti-tumor assessment also demonstrated that Tat-FeAgNP-Dox displayed the most significant tumor-inhibiting effects and reduced the specific growth rate of tumor by 29.6%(P ? 0.009), which could be attributed to its superior performance in tumor drug delivery in comparison with the control nanovehicles. | Ergang Liu Meng Zhang Hui Cui Junbo Gong Yongzhuo Huang Jianxin Wang Yanna Cui Weibing Dong Lu Sun Huining He Victor C.Yang | 2018 | Acta Pharmaceutica Sinica B2018，8，6： | 5 |
| 3 | Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions显示文摘 | Yanna Liang Nicolas Sarkany Yi Cui | 2009 | Biotechnology Letters2009，，7： | 2 |
| 4 | Biomass and lipid productivities of Chlorella vulgaris under autotrophic, het- erotrophic and mixotrophic growth conditions 显示文摘 | LIANG Yanna SARKANY N CUI Yi | 2009 | Biotechnology Letters2009，31，7： | 1 |
| 5 | Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions显示文摘 | Yanna Liang Nicolas Sarkany Yi Cui | 2009 | Biotechnology Letters2009，，7： | 1 |
| 6 | Biomass and lipid productivities of Chlorella vulgaris under au- totrophic,heterotrophic and mixotrophic growth condi- tions显示文摘 | YANNA LIANG NICOLAS SARKANY YI CUI | 2009 | Biotechnol Lett2009，31，7： | 1 |
| 7 | Construction of Ad-EGFP-BDNF vector and its expression in neural stem cells显示文摘BACKGROUND:Brain-derived neurotrophic factor(BDNF) provides nourishment to injured neurons.Neural stem cells can differentiate into neurons to repair neuronal injury in vivo.It has been hypothesized that continuous secretion of BDNF from neural stem cells could benefit brain injury repair.OBJECTIVE:To transfect BDNF and enhanced green fluorescent protein(EGFP) into neural stem cells with adenovirus vector and to observe expression of BDNF and EGFP in transfected neural stem cells.DESIGN,TIME AND SETTING:Observational,cellular,molecular study was performed at the Biochemistry Laboratory,Tongji University School of Medicine,China from July 2004 to September 2006.MATERIALS:Neural stem cells were provided by the Anatomy and Histoembryology Laboratory of Fudan University Medical School,China.METHODS:BDNF cDNA was extracted by reverse transcription polymerase chain reaction from the rat hippocampus.Following gene cloning and packaging by HEK293.BDNF,the EGFP gene was transfected into cultured neural stem cells with the Ad-EGFP-BDNF vector.BDNF-expressing neural stem cell clones were selected by G418 selection.MAIN OUTCOME MEASURES:EGFP expression and cell morphology were observed by fluorescent microscopy;neural stem cell expressing BDNF mRNA was examined by reverse transcription polymerase chain reaction;BDNF expression was detected by enzyme-linked immunosorbent assay from supernatant of infected neural stem cells.RESULTS:High transfection efficiency was obtained using 5 × 108 virus titers to transfect neural stem cells.G418-resistant neural stem cell clones integrated BDNF mRNA fragments.Enzyme-linked immunosorbent assay results showed that BDNF expression in the supernatant increased with increasing culture time and peaked at 72 hours.CONCLUSION:Adenovirus-mediated BDNF and EGFP genes were successfully transfected into neural stem cells and were expressed in neural stem cells for a long period of time. | Shaohua Li Zhenhua Li Huilong Wen Xin Gu Yinjiang Cui Yang Wang Lixia Lu Yanna Li Manli Na | 2010 | Neural Regeneration Research2010，5，13： | 1 |
| 8 | Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heter- otrophic and mixotrophic growth conditions 显示文摘 | LIANG Yanna SARKANY N CUI Yi | 2009 | Biotechnol Lett2009，31，7： | 1 |
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