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| 1 | Artificial evolution of OsEPSPS through an improved dual cytosine and adenine base editor generated a novel allele conferring rice glyphosate tolerance显示文摘Exploiting novel endogenous glyphosate-tolerant alleles is highly desirable and has promising potential for weed control in rice breeding. Here,through fusions of different effective cytosine and adenine deaminases with nCas9-NG, we engineered an effective surrogate two-component composite base editing system, STCBE-2, with improved C-to-T and A-to-G base editing efficiency and expanded the editing window. Furthermore,we targeted a rice endogenous OsEPSPS gene for artificial evolution through STCBE-2-mediated near-saturated mutagenesis. After hygromycin and glyphosate selection, we identified a novel OsEPSPS allele with an Asp-213-Asn(D213N)mutation(OsEPSPS-D213N) in the predicted glyphosate-binding domain, which conferred rice plants reliable glyphosate tolerance and had not been reported or applied in rice breeding. Collectively, we developed a novel dual base editor which will be valuable for artificial evolution of important genes in crops. And the novel glyphosate-tolerant rice germplasm generated in this study will benefit weeds management in rice paddy fields. | Chen Zhang Xue Zhong Shaoya Li Lei Yan Jingying Li Yubing He Yong Lin Yangjun Zhang Lanqin Xia | 2023 | Journal of Integrative Plant Biology2023，65，9： | 1 |
| 2 | Fluorene-based narrow-band-gap copolymers for red lightemitting diodes and bulk heterojunction photovoltaic cells显示文摘A series of narrow band-gap conjugated copolymers(PFO-DDQ)derived from 9,9-dioctylfluorene(DOF)and 2,3-dimethyl-5,8-dithien-2-yl-quinoxalines(DDQ)is prepared by the palladium-catalyzed Suzuki coupling reaction with the molar feed ratio of DDQ at around 1%,5%,15%,30%and 50%,respectively.The obtained polymers are readily soluble in common organic solvents.The solutions and the thin solid films of the copolymers absorb light from 300-590 nm with two absorbance peaks at around 380 and 490 nm.The intensity of 490 nm peak increases with the increasing DDQ content in the polymers.Efficient energy transfer due to exciton trapping on narrow-band-gap DDQ sites has been observed.The PL emission consists exclusively of DDQ unit emission at around 591-643 nm depending on the DDQ content in solid film.The EL emission peaks are red-shifted from 580 nm for PFO-DDQ1 to 635 nm for PFO-DDQ50.The highest external quantum efficiency achieved with the device configuration ITO/PEDOT/PVK/PFO-DDQ15/Ba/Al is 1.33%with a luminous efficiency 1.54 cd/A.Bulk heterojunction photovoltaic cells fabricated from composite films of PFO-DDQ30 copolymer and[6,6]-phenyl C61 butyric acid methyl ester(PCBM)as electron donor and electron acceptor,respectively in device configuration:ITO/PEDOT:PSS/PFODDQ30:PCBM/PFPNBr/Al shows power conversion efficiencies of 1.18%with open-circuit voltage(V_(oc))of 0.90 V and short-circuit current density(J_(sc))of 2.66 mA/cm^(2)under an AM1.5 solar simulator(100 mW/cm^(2)).The photocurrent response wavelengths of the PVCs based on PFO-DDQ30/PCBM blends covers 300-700 nm.This indicates that these kinds of low band-gap polymers are promising candidates for polymeric solar cells and red light-emitting diodes. | Mingliang SUN Li WANG Yangjun XIA Bin DU Ransheng LIU Yong CAO | 2008 | Frontiers of Chemical Science and Engineering2008，2，3： | 0 |
| 3 | Engineering a plant A-to-K base editor with improved performance by fusion with a transactivation module显示文摘Dear Editor,Base editors(BEs)are promising tools that enable single-nucleotide substitutions in specific target genes,generating loss-offunction or gain-of-function mutations,which can greatly accelerate plant functional genomics and crop improvement(Ren et al.,2018;Zeng et al.,2020;Xu et al.,2021;Yan et al.,2021;Li et al.,2023).To date,three major classes of BEs have been engineered:cytosine BEs(CBEs)for C-to-T transitions(Komor et al.,2016),adenine BEs(ABEs)for A-to-G transitions(Gaudelli et al.,2017). | Yucai Li Shaoya Li Chenfei Li Chen Zhang Lei Yan Jingying Li Yubing He Yan Guo Yong Lin Yangjun Zhang Lanqin Xia | 2023 | Plant Communications2023，4，6： | 0 |
| 4 | A new photolabeling probe for efficient enrichment and deep profiling of cell surface membrane proteome by mass spectrometry显示文摘The cell surface membrane proteome is a class of proteins encoded by ~25% of all protein-coding genes in living organisms and plays a key role in mediating communication between the cells and their surrounding environment. However, most cell surface membrane proteins(CSMPs) are naturally expressed at very low levels compared with intracellular proteins. The difficulties in their purification with high specificity further hinder the understanding of their structure and function. In this study, we developed a new photolabeling probe to achieve efficient tagging and facile enrichment of the CSMPs. The probe is composed of a lipid tail for cell surface localization, a polyethylene glycol(PEG) spacer for increased water solubility, two 4-(N-maleimido)benzophenone(MBP) groups for UV-active tagging of the CSMPs, and a biotin tag for subsequent isolation. Application of this photolabeling probe resulted in the successful enrichment and identification of 3098 annotated CSMPs in HT22 cells with close to 70% selectivity. The proposed photolabeling probe and enrichment strategy were demonstrated to be a powerful method for deep cell surface proteome profiling, representing one of the largest groups of current drug targets. | Yuanyuan Li Chaoshuang Xia Hongxian Zhao Yuping Xie Yangjun Zhang Wanjun Zhang Yongliang Yu Jianhua Wang Weijie Qin | 2023 | Chinese Chemical Letters2023，34，2： | 0 |
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