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| 1 | Prokaryotical expression of structural and non-structural proteins of hepatitis G virus显示文摘AIM To study the epitope distribution of hepatitis G virus (HGV) and to seek for the potential recombinant antigens for the development of HGV diagnositic reagents.METHODS Fourteen clones encompassing HGV gene fragments from core to NS3 and NS5 were constructed using prokaryotic expression vector pRSET and (or)pGEX. and expressed in E. coli. Western blotting and ELISA were used to detect the immunoreactivity of these recombinant proteins.``RESULTS One clone with HGV fragment from core to El(Gl). one from E2 (G31), three from NS3 (G6, G61, G7),one from NS5B (G821) and one chimeric fragment from NS3and NS5B (G61 821) could be expressed well and showed obvious immunoreactivity by Western blotting.One clone with I-KGV framment from NS5B (G82) was also well expressed, but could not show immunoreactivity by Western blotting. No obvious expression was found in the other six clones. All the expressed recombinant proteins were in inclusion body form, except the protein G61 which could be expressed in soluble form. Further purified recombinant proteins Gl, G,31, G61, G821 and G61 821were detected in indirected ELISA as coating antigen respectively. Only recombinant Gl could still show immunoreactivity, and the other four recombinant proteins failed to react to the HGV antibody positive sera.Western blotting results indicated that the immunoactivity of these four recombinant proteins were lost during purification.``CONCLUSION Core to El, E2. NS3 and NS5 fragment of HGV contain antigenic epitopes, which could be produced in prokaryotically expressed recombinant proteins. A high. yield recombinant protein (Gl) located in HGV core to E1 could remain its epitope after purification, which showed the potential that G1 could be used as a coating antigen to develop an ELISA kit for HGV specific antibody diagnosis. | Ning-Shao Xia~1 Hai-Jie Yang~1 Jun Zhang~1 Chang-Qing Lin~1 Ying-Bin Wang~1 Juan Wang~1 Mei-Yun Zhan~2 MH Ng~3 1 Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering,Xiamen University,Xiamen 361005,Fujian Province,China2 Institute of Virology,Chinese Academy of Preventive Medicine Beijing 100052,China3 Department of Microbiology,Hoog Kong University,Hongkong,China | 2001 | World Journal of Gastroenterology2001，7，5： | 4 |
| 2 | 一般人群中腹型肥胖和新发心房颤动的关系显示文摘已知身高越高,体质量越大,心房颤动发生风险越高。然而,心房颤动的风险是否与腹型肥胖有关尚不清楚。Baek等纳入韩国国民健康保险计划全国样本队列中基线无心房颤动的成人501 690人,年龄(47.6±14.3)岁,女性250 664人,占50%。测定其体质量指数(body mass index,BMI)和腰围,将BMI〈18.5kg/m2定义为低体质量,18.5~〈25.0kg/m2定义为正常体质量;25.0~〈30.0kg/m2定义为超重;≥30.0kg/m2定义为肥胖;将腰围≥90(男性)和≥80cm(女性)定义为腹型肥胖。 | 刘莉 叶鹏 Baek YS Yang PS Kim TH Uhm JS Park J Pak HN Lee MH Joung B | 2017 | 中华高血压杂志2017，25，9： | 4 |
| 3 | Preliminary results of integrated therapy for patients with knee osteoarthritis显示文摘 | Huang MH Yang RC Lee CL | | Arthritis and Rheumatism0，，： | 2 |
| 4 | Incidence and determinants of spontaneous hepatitis B surface antigen seroclearance: a community- based foltow-up study 显示文摘 | Liu J Yang HI Lee MH | 2010 | Gastroenterology2010，139，： | 1 |
| 5 | Enhanced NMDA receptor-media- ted synaptic transmission, enhanced long-term potentiation, and im- paired learning and memory in mice lacking IRSp53 显示文摘 | Kim MH Choi J Yang J | 2009 | J Neu~os- ci2009，29，5： | 1 |
| 6 | Cloning differentially expressed genes by linker capture subtraction显示文摘 | Yang MH Sytkowski AJ | 1996 | Anal Biochem1996，237，： | 1 |
| 7 | Changes in Ca, Cu, Fe, Mg, and Zn contents in mouse brain tissues after prolonged oral ingestion of brick tea liquor containing a high level of AI 显示文摘 | Yang MS Wong MH | 2001 | Biol Trace Elem Res2001，80，1： | 1 |
| 8 | Radiation therapy versus chemotherapy as initial treatment for localized nasal natural killer (NK)/T-cell lymphoma:a single institute survey in Taiwan显示文摘 | You JY Chi KH Yang MH | 2004 | Ann Oncol2004，15，4： | 1 |
| 9 | Placental and membrane aquaporin water channels:correlation with amniotic fluid volume and composition显示文摘 | Beall MH Wang S Yang B | 2006 | Placenta2006，，： | 1 |
| 10 | Hydrogen peroxide 2 induced cell death in a human retinal pigment epithelial cell line, ARPE219 显示文摘 | Kim MH Chung J Yang JW | 2003 | Korean J Oph thalmol2003，17，1： | 1 |
| 11 | The significance of blood pressure variability for the development of hemor- rhagic transformationin acute ischemic stroke 显示文摘 | Ko Y Park JH Yang MH | 2010 | Stroke2010，41，11： | 1 |
| 12 | Comprehensive analysis of the independent effect of twist and snail in promoting metastasis of hepatocellular carcinoma 显示文摘 | Yang MH Chen CL Chau GY | 2009 | Hepatology2009，50，5： | 1 |
| 13 | The role of contrast-enhanced ultrasound in planning treatment protocols for hepatocellular carcinoma before radiofrequency ablation 显示文摘 | Chen MH Yang W Yan K | 2007 | Clin Radiol2007，62，8： | 1 |
| 14 | The association of serum lipids with coloreetal adenomas 显示文摘 | Yang MH Rampal S Sung J | 2013 | Am J Gastroenterol2013，108，5： | 1 |
| 15 | MALATl promotes colorectal cancer cell proliferation/migration/invasion via PRKA kinase anchor protein 9显示文摘 | Yang MH Hu ZY Xu C | 2015 | Biochim Biophys Acta2015，1852，1： | 1 |
| 16 | Effects of propofol on nociceptive respone and power spectra of electroencephalographic and systemic arterial pressure signals in the ral: correction with plasmas concentration显示文摘 | Yang CH Shyr MH Kuo TB | 1995 | J Pharmacol Exp Ther1995，2，7： | 1 |
| 17 | Fibrosis of collagen I and remodeling of connexin 43 in atrial myocardium of patients with atrial fibrillation显示文摘 | Luo MH Li YS Yang KID | 2007 | Cardiology2007，107，4： | 1 |
| 18 | Comparison of tuberculosis and pyogenic epididymal abscesses:clinical gray-scale sonographic,and color Doppler sonographic features显示文摘 | Yang DM Yoon MH Kim HS | 2001 | AJR Am J Roentgenol2001，177，5： | 1 |
| 19 | The role of contrast-enhanced ultrasound in planning treatment protocols for hepatocellular carcinoma before radiofrequency ablation显示文摘 | Chen MH Yang W Yan K | | 0，，： | 1 |
| 20 | The differential expression of intercellular adhesion molecule -1 (ICAM-1) and regulation by interferon gamma during the pathogenesis of endometriosis 显示文摘 | Wu MH Yang BC Lee YC | 2004 | Am J Reprod Immunol2004，51，5： | 1 |
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