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| 1 | Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma显示文摘BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.METHODS:A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression.Total RNA was purified and transcribed into cDNA by reverse transcription.Fragments of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by sequencing.GPC-3 was analyzed by immunohistochemistry,Western blotting or ELISA.RESULTS:Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm.Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT expression.The incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,respectively.There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,P<0.05) or liver GPC-3 (r=1.0,P<0.001) or serum GPC-3 (r= 0.994,P<0.001).CONCLUSION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC. | Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,Sai WL,Yang JL and Zhang HJ) Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ) Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG) | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 51 |
| 2 | Vascular endothelial growth factor receptor 2(VEGFR-2) plays a key role in vasculogenic mimicry formation,neovascularization and tumor initiation by Glioma stem-like cells显示文摘Human glioblastomas(GBM)are thought to be initiated by glioma stem-like cells(GSLCs).GSLCs also participate in tumor neovascularization by transdifferentiating into vascular endothelial cells.Here,we report a critical role of GSLCs in the formation of vasculogenic mimicry(VM),which defines channels lined by tumor cells to supply nutrients to early growing tumors and | Yao X Ping Y Liu Y Chen K Yoshimura T Liu M Gong W Chen C Niu Q Guo D Zhang X Wang JM Bian X | 2013 | 中国神经肿瘤杂志2013，11，3： | 23 |
| 3 | 功能的限制的积极选择而非松驰在鸡驯服期间驾驶视觉的进化显示文摘是由达尔文,注意了鸡有所有鸟,而是国内鸡和他们的野祖先之间的有趣的进化差别的最大的 phenotypic 差异,红 Junglefowl,是他们的比较地更弱的视觉。存在理论建议在国内鸡之中的减少的视觉英勇反映功能的限制的松驰在识别负责的内在的基因机制的视觉,而是证据上驾驶的变化因为这个变化决定性地没被描绘。这里,国内鸡的染色体宽的分析和红 Junglefowl 染色体为涉及视觉的发展的断然选择的基因显示出重要丰富。关于为在视网膜的这些基因的 mRNA 表示的水平国内鸡和他们的野祖先之间有重要差别。涉及视觉的发展的众多的另外的基因也在在国内鸡和他们的野祖先之间的 mRNA 表示显示出重要差别,特别地为与 phototransduction 和光敏电阻器开发联系的基因,例如 RHO (视紫红质) , GUCA1A, PDE6B 和 NR2E3。最后,我们在视觉描绘了 VIT 基因的潜在的角色,它在村庄鸡的视网膜经历了积极选择和 downregulated 表示。总的来说,我们的结果建议净化选择的那种积极选择,而非松驰,在国内鸡贡献了视觉的进化。怀有更弱的视觉的国内鸡的祖先可能显示出减少的害怕回答和警戒,使他们更安逸无意识地被选择或驯养。 | Ming-Shan Wang Rong-wei Zhang Ling-Yan Su Yah Li Min-Sheng Peng He-Qun Liu Lin Zeng David M Irwin Jiu-Lin Du Yong-Gang Yao Dong-Dong Wu Ya-Ping Zhang | 2016 | Cell Research2016，26，5： | 15 |
| 4 | 2020年日本胃肠内镜学会(JGES)《早期胃癌ESD和EMR指南》要点显示文摘日本胃肠内镜学会(The Japan Gastroenterological Endoscopy Society,JGES)最近发布了2020年版《早期胃癌内镜黏膜下剥离(endoscopic submucosal dissection,ESD)和内镜下黏膜切除(endoscopic mucosal resection,EMR)指南》,根据近年来大量新的研究成果,对2014年发布的《早期胃癌ESD及EMR指南》进行更新和充实。 | 凌世宝 王业涛 Ono H Yao K Fujishiro M | 2021 | 胃肠病学和肝病学杂志2021，30，3： | 9 |
| 5 | 笋芽顶端分生组织在竹秆横切面形态形成中的作用与机制研究显示文摘竹秆的横切面主要由表皮、皮层、维管组织、基本组织及髓腔(早期为髓组织)组成。这些组织均由笋芽茎顶端分生组织分化发育而来,且其在生长发育过程中促使了竹秆横切面形态(本文特指:竹秆横切面外轮廓形状、围度及壁-腔结构)的形成。然而,人们对于这一生长发育过程的生物学机制知之甚少。本研究利用解剖学、数学模拟等方法,对 20 种具不同秆径竹种的笋芽顶端分生组织进行了分析。研究结果发现,笋芽顶端分生组织指状形态的维持是竹子产生圆柱形竹秆的基础;在顶端分生组织细胞数目增加的同时,线性增加原体细胞数目应是竹子在演化过程中产生大径竹秆且保持规则壁-腔结构的方式。进一步通过研究一个竹壁增厚且壁-腔架构紊乱的稳定变异体———实肚竹( Phyllostachys nidularia f. farcta)发现,其笋芽顶端分生组织细胞数目减少且原体/原套细胞数比值降低使其产生了高比例的壁组分组织(指基本分生组织与肋状分生组织)与低比例的髓组织。上述 2 种组织的比例失衡,使得实肚竹笋芽在后续发育中壁组分组织随机挤入到了髓组织中,最终形成了实肚竹异常的竹秆横切面形态(竹壁多样化增厚且壁-腔结构紊乱)。此外,相对较小的顶端分生组织,使得实肚竹笋芽产生了较少的内源激素,如其生长素含量仅约为其原种篌竹的 67. 5%,各细胞分裂素含量也显著低于篌竹笋芽。转录组分析进一步发现,在实肚竹笋芽中与激素及代谢相关的功能基因呈显著下调表达。这些生理与分子变化最终导致了实肚竹竹秆矮化、秆径减小且生物量降低。本论文的研究结果为研究竹秆横切面发育提供了一个重要的视角,同时也为实肚竹厚壁茎秆的形成提出了一个合理的机制。 | Wang Y Sun X Ding Y Fei Z Jiao C Fan M Yao B Xin P Chu J Wei Q 王雨珺 魏强 | 2019 | 世界竹藤通讯2019，17，3： | 5 |
| 6 | Bupivacaine causes cytotoxicity in mouse C2C12 myoblast cells: involvement of ERK and Akt signaling pathways显示文摘 | Joseph M MAURICE Yan GAN Fan-xin MA Yong-chang CHANG Michael HIBNER Yao HUANG | 2010 | Acta Pharmacologica Sinica2010，31，4： | 5 |
| 7 | First-Principles Study of Phase Stability and Mechanical Property of Co_3(Al,W) Precipitates with Different Ratio of Al to W in Co Base Alloys显示文摘The energetics, electronic structure and mechanical property of Co3(Al,W) precipitates with different ratio of Al to W in Co base alloys were investigated by the method of supercell and augmented plane waves plus local orbitals within generalized gradient approximation. The calculated results show that the L12 Co3(Al,W) precipitate is the most stable when the ratio of Al to W is equal to 1. When the content of W is higher than 18.5 at%, the L12 and hexagonal structures co-exist in Co3(Al,W) precipitates at 1173 K. It is also shown that the L12 ordered Co3(Al,W) precipitates have an obvious strengthening effect in the disordered fcc cobalt matrix. | Yao Q Su X M Yan Y Zhao L W Sun J | 2009 | 稀有金属材料与工程2009，38，A03： | 3 |
| 8 | Investigation of microstructure modification of C-doped a-SiO_2/Si after Pb-ion irradiation显示文摘Thermally grown amorphous SiO 2 (a-SiO 2) films were implanted at room temperature (RT) with 100 keV C-ions to 5.0×10 17 ions/cm 2.These samples were irradiated at RT with 853 MeV Pb-ions to 1.0×10 12 and 5.0×10 12 ions/cm 2.Then the samples were investigated using Transmission Electron Microscopy (TEM) at RT.Significant microstructure modifications were observed in C-doped a-SiO 2/Si samples after high energy Pb-ion irradiations,and the formation of new structures depended strongly on the Pb-ion irradiation fluences.For example,tracks in high density were observed in a 1.0×10 12 Pb/cm 2 irradiated and C-doped sample.Additionally,the length of tracks grows,and a large number of 8H-SiC nanocrystals can be seen in the film when irradiation fluence is increased to 5.0×10 12 Pb/cm 2.Possible modification processes of C-doped a-SiO 2 under swift heavy ion irradiations are briefly discussed. | LIU ChunBao WEI KongFang YAO CunFeng WANG ZhiGuang JIN YunFan TOULEMONDE M | 2012 | Science China(Physics,Mechanics & Astronomy)2012，55，2： | 2 |
| 9 | Seismic design and analysis of underground structures显示文摘 | Hashash T M A Hook J J Schmidt B Yao J I | 2001 | Tunnelling and Underground Space Technology2001，16，4： | 2 |
| 10 | Association between big endothelin-1 and CHADS2/CHA2DS2-VASc scores in non-valvular atrial fibrillation显示文摘Background Endothelial function,as measured by big endothelin-1(ET-1),has been demonstrated to be useful in predicting adverse long-term events in patients with cardiovascular disease.Nevertheless,there are little data about the association between big ET-1 and thromboembolism risk in atrial fibrillation(AF).We aimed to investigate the relationship between big ET-1 and CHADS2/CHA2DS2-VASc scores used for evaluating thromboembolic risk in patients with non-valvular AF.Methods The study population consisted of 238 consecutive AF patients(67.6%with paroxysmal AF and 32.4%with persistent AF).The patients were divided into two groups(high-or low-intermediate risk group)based on CHADS2 and CHA2 DS2-VASc scores(score≥2 or<2,respectively).Clinical,laboratory,and echocardiographic parameters were evaluated,and the CHADS2/CHA2DS2-VASc scores were compared between groups.The association between big ET-1 levels and CHADS2/CHA2DS2-VASc score was assessed.Multivariate logistic regression analysis was performed to identify independent predictors of CHADS2/CHA2DS2-VASc scores.Results The high CHADS2/CHA2DS2-VASc score group had older age,higher big ET-1 levels,and enlarged left atrial diameter than the low CHADS2/CHA2DS2-VASc score group(P<0.05).Multiple logistic regression analysis revealed that big ET-1 level was an independent determinant of high CHADS2/CHA2DS2-VASc scores[odds ratio(OR)=2.545 and OR=3.816;both P<0.05].Conclusions Our study indicates that in non-valvular AF,big ET-1 was significantly correlated with CHADS2/CHA2DS2-VASc scores and an independent predictor of high CHADS2/CHA2DS2-VASc scores.Big ET-1 may serve as a useful marker for risk stratification in this setting. | Li-Hui ZHENG Shang-Yu LIU Feng HU Er-Peng LIANG Ling-Min WU Hong-Lei WANG Ammar M Killu Yan YAO | 2019 | Journal of Geriatric Cardiology2019，16，11： | 2 |
| 11 | Hepatocytes isolated from neoplastic liver-immunomagnetic purging as a new source for transplantation显示文摘AIM: To investigate whether hepatocytes isolated from macroscopically normal liver during hepatic resection for neoplasia could provide a novel source of healthy hepatocytes, including the development of reliable protocols for malignant cells removal from the hepatocyte preparation. METHODS: Hepatocytes were procured from resected liver of 18 patients with liver tumors using optimised digestion and cell-enrichment protocols. Suspensions of various known quantities of the HT-29 tumor cell line and patient hepatocytes were treated or not with Ep-CAM-antibody-coated immunomagnetic beads in order to investigate the effi cacy of tumor-purging by immunomagnetic depletion, using a semi-quantitative RT-PCR method developed to detect tumor cells. Immunomagnetic bead-treated or bead-untreated tumor cell-hepatocyte suspensions were transplanted intra-peritoneally in Balb/C nude mice to assess the rates of tumor development. RESULTS: Mean viable hepatocyte yield was 9.3 x 106 cells per gram of digested liver with mean viability of 70.5%. Immunomagnetic depletion removed tumor cells to below the RT-PCR detection-threshold of 1 tumor cell in 106 hepatocytes, representing a maximum tumor purging efficacy of greater than 400 000-fold. Transplanted, immunomagnetic bead-purged tumor cell-hepatocyte suspensions did not form peritonealtumors in Balb/C nude mice. Co-transplantation of hepatocytes with tumor cells did not increase tumorigenesis of the tumor cells. CONCLUSION: Immunomagnetic depletion appears to be an effective method of purging contaminating tumor cells to below threshold for likely tumorigenesis. Along with improved techniques for isolation of large numbers of viable hepatocytes, normal liver resected for neoplasia has potential as another clinically useful source of hepatocytes | Aravin Gunasegaram Javed Akhter Peng Yao Loreena A Johnson Stephen M Riodan David L Morris | 2008 | World Journal of Gastroenterology2008，14，32： | 2 |
| 12 | Effect of poxB gene knockout on metabolism in Escherichia coli based on growth characteristics and enzyme activities显示文摘 | Li, M Yao, SJ Shimizu, K | 2007 | 中国生物学文摘2007，21，10： | 2 |
| 13 | Sonochemical Synthesis of Highly Luminescent Zinc Oxide Nanoparticles Doped with Magnesium(II)显示文摘 | Huan‐Ming Xiong Dmitry G. Shchukin Helmuth M?hwald Yang Xu Yong‐Yao Xia | 2009 | Angew. Chem. Int. Ed2009，，15： | 2 |
| 14 | Equilibria,kinetics,and mechanism in the bicarbonate activation of hydrogen peroxide: oxidation of sulfides by peroxymonocarbonate显示文摘 | RICHARDSON D E YAO H FRANK K M | 2000 | J Am Chem Soc2000，122，： | 1 |
| 15 | Estrogen regulates Bcl-w and Bim expression: role in protection against beta-amyloid peptide-induced neuronal death 显示文摘 | Yao M Nguyen TV Pike CJ | 2007 | J Nearosci2007，27，6： | 1 |
| 16 | Removal and recovery of phosphate and ammonium as struvite from supernatant in anaerobic dlgestion显示文摘 | Yoshino M Yao M Tsuno H | 2003 | Water Sci Tech2003，48，2： | 1 |
| 17 | PTEN/MMAC1/TEP1 mutations in human primary renal cell carcinomas and renal carcinoma cell lines显示文摘 | Kondo K Yao M Kobayashi K | 2001 | Int J Cancer2001，91，2： | 1 |
| 18 | Controlling shrinkage in wool fabrics: effective hydrogen peroxide systems 显示文摘 | CARDAMONE J M YAO J NUNEZ A | 2004 | Textile Research Journal2004，74，10： | 1 |
| 19 | Rigid polyurethane foams from combined liquefaction mixtures of wood and starch 显示文摘 | Yao Y G Yoshioka M Shiraishi N | 1995 | Mokuzai Gakkaishi1995，41，7： | 1 |
| 20 | The resistance to wear and corrosion of laser-cladding Al2O3 ceramic coating on Mg alloy显示文摘 | Gao Y Wang C Yao M | 2007 | Applied Surface Science2007，253，12： | 1 |
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