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| 1 | An Effective Method for Total RNA Isolation from Bamboo显示文摘Trizol reagent was used for RNA isolation from fresh leaves of Dendrocalamopsis oldhami, Bambusa ventricosa and Phyllostachys aureosulcata cv. Pekinensis. The extracted RNA from leaves had the normal ultraviolet absorption, the value of OD260/OD280 varied between 1.8-2.0. The 28s rRNA was more than two times brighter than 18s rRNA in electrophoresis. These results indicated that the total RNA was complete and not degraded. According to our experiments, RNA could be obtained simply with this method, and can be used for molecular manipulation such as cDNA synthesis and gene cloning. | GAO Zhimin LI Xueping LI Lubin PENG Zhenhua | 2006 | Chinese Forestry Science and Technology2006,5,3: | 39 |
| 2 | Co-occurrence Patterns of Above-ground and Below-ground Mite Communities in Farmland of Sanjiang Plain, Northeast China显示文摘One of the fundamental questions in community ecology is whether communities are random or formed by deterministic mechanisms. Although many efforts have been made to verify non-randomness in community structure, little is known with regard to co-occurrence patterns in above-ground and below-ground communities. In this paper, we used a null model to test non-randomness in the structure of the above-ground and below-ground mite communities in farmland of the Sanjiang Plain, Northeast China. Then, we used four tests for non-randomness to recognize species pairs that would be demonstrated as significantly aggregated or segregated co-occurrences of the above-ground and below-ground mite communities. The co-occurrence pattern of the above-ground mite community was significantly non-random in October, suggesting species segregation and hence interspecific competition. Additionally, species co-occurrence patterns did not differ from randomness in the above-ground mite community in August or in below-ground mite communities in August and October. Only one significant species pair was detected in the above-ground mite community in August, while no significant species pairs were recognized in the above-ground mite community in October or in the below-ground mite communities in August and October. The results indicate that non-randomness and significant species pairs may not be the general rule in the above-ground and below-ground mite communities in farmland of the Sanjiang Plain at the fine scale. | LIN Lin GAO Meixiang LIU Dong ZHANG Xueping WU Haitao WU Donghui | 2014 | Chinese Geographical Science2014,24,3: | 7 |
| 3 | Gene characterization and molecular pathway analysis of reverse thermosensitive genic male sterility in eggplant(Solanum melongena L.)显示文摘The naturally occurring mutant eggplant line 05ms was identified with reverse thermosensitive genic male sterility(rTGMS),but its temperature-responsive fertility mechanisms remain largely unknown.Here,we studied the flower morphology,anther cellular structure,and genome-wide gene expression of this rTGMS line.Candidate genes for thermosensitive male sterility during the microspore development of 05ms and the temperature-insensitive line S63 under low-temperature(LT)and high-temperature(HT)conditions were identified.Under LT,tapetum cells were vacuolated and had delayed disintegration in 05ms.RNA-seq analysis indicated that DEGs were enriched in the KEGG pathways‘plant hormone signal transduction’,‘starch and sucrose metabolism’,and‘phenylpropanoid biosynthesis’.We identified two genes,4CLL1(Sme2.5_00368.1_g00010.1)and CKI1(Sme2.5_10056.1_g00002.1),which could potentially regulate eggplant anther development and may be candidate genes for rTGMS.Finally,we propose a working model of anther abortion for rTGMS in eggplant.CKI1 responds to LT stress and causes expression changes in genes related to anther development,such as 4CLL1,and the cellular structure of the tapetum becomes abnormal,causing male sterility.The findings of this study explain the underlying molecular mechanisms of male sterility in eggplant rTGMS lines. | Bing Li Xueping Chen Yanrong Wu Aixia Gu Jingjing Zhang Shuangxia Luo Xiurui Gao Jianjun Zhao Xiuqing Pan Shuxing Shen | 2019 | Horticulture Research2019,6,1: | 5 |
| 4 | The characteristic and prediction of scour in front of breakwaters by standing waves显示文摘Thecharacteristicandpredictionofscourinfrontofbreakwatersbystandingwaves¥GaoXueping(DeptartmentofWaterResourcesandHarborEngin... | Gao Xueping(Deptartment of Water Resources and Harbor Engineering, Tianjin University, Tianjin 300072, China) | 1995 | Acta Oceanologica Sinica1995,14,1: | 4 |
| 5 | Responses of Soil Fauna Structure and Leaf Litter Decomposition to Effective Microorganism Treatments in Da Hinggan Mountains,China显示文摘Microorganisms are nutritious resources for various soil fauna.Although soil fauna grazing affects microorganism composition and decomposition rate,the responses of soil fauna and leaf litter decomposition to added microorganism is little understood.In this study,in the coniferous and broad-leaved mixed forest of Tahe County in the northern Da Hinggan Mountains,China,three sampling sites(each has an area of 10 m2) were selected.The first two sites were sprinkled with 250 times(EM1) and 1000 times(EM2) diluted effective microorganism(EM) preparations evenly,and the third site was sprinkled with the same volume of water as a control site.The responses of soil fauna structure and leaf litter decomposition to EM treatment were conducted during three years.The results revealed that EM treatment resulted in significant increase of soil organic matter.The number of soil fauna in the EM1 and EM2 sites increased by 12.88% and 2.23% compared to the control site,and among them springtails and mites showed the highest increase.However,the groups of soil fauna in the EM1 and EM2 sites decreased by 6 and 9,respectively.And the changes in the diversity and evenness index were relatively complicated.EM treatment slowed the decomposition of broad-leaved litter,but accelerated the decomposition of coniferous litter.However,the decomposition rate of broad-leaved litter was still higher than that of coniferous litter.The results of this study suggested that the added microorganisms could help individual growth of soil fauna,and this method led to a change in the process of leaf litter decomposition.This paper did not analyze the activity of soil microorganisms,thus it is difficult to clearly explain the complex relationships among litter type,soil fauna and soil microorganisms.Further research on this subject is needed. | GAO Meixiang LI Jingke ZHANG Xueping | 2012 | Chinese Geographical Science2012,22,6: | 4 |
| 6 | INTERACTION BETWEEN WAVES,STRUCTURES AND SAND BEDS显示文摘INTERACTIONBETWEENWAVES,STRUCTURESANDSANDBEDS¥GaoXue-ping(Dept.ofWaterResources&HarbourEng.,TianjinUniv.,Tianjin,300072,P.R.C... | Gao, Xueping Zhao, Zidan | 1995 | Journal of Hydrodynamics1995,7,4: | 4 |
| 7 | Distribution of soil aggregates and organic carbon in deep soil under long-term conservation tillage with residual retention in dryland显示文摘To ascertain the effects of long-term conservation tillage and residue retention on soil organic carbon(SOC) content and aggregate distribution in a deep soil(>20-cm depth) in a dryland environment,this paper analyzed the SOC and aggregate distribution in soil, and the aggregate-associated organic carbon(OC) and SOC physical fractions. Conservation tillage(reduced tillage with residue incorporated(RT) and no-tillage with residue mulch(NT)) significantly increased SOC sequestration and soil aggregation in deep soil compared with conventional tillage with residue removal(CT). Compared with CT, RT significantly increased the proportion of small macroaggregates by 23%–81% in the 10–80 cm layer, and the OC content in small macroaggregates by 1%–58% in the 0–80 cm layer. RT significantly increased(by 24%–90%) the OC content in mineral-SOC within small macroaggregates in the 0–60 cm layer, while there was a 23%–80% increase in the 0–40 cm layer with NT. These results indicated that:(1) conservation tillage treatments are beneficial for soil aggregation and SOC sequestration in a deep soil in a dryland environment; and(2)the SOC in mineral-associated OC plays important roles in soil aggregation and SOC sequestration. In conclusion, RT with NT is recommended as an agricultural management tool in dryland soils because of its role in improving soil aggregation and SOC sequestration. | WANG Bisheng GAO Lili YU Weishui WEI Xueqin LI Jing LI Shengping SONG Xiaojun LIANG Guopeng CAI Dianxiong WU Xueping | 2019 | Journal of Arid Land2019,11,2: | 3 |
| 8 | Phase–amplitude model for doubly fed induction generators显示文摘The doubly fed induction generator(DFIG) is major type of wind turbine generator used in grid-connected wind farms. Practical models of DFIG have been built to study the influence of wind power generation on power system dynamics. However, most existing practical models of the DFIG are based on rectangular coordinates,in which frequency variation is neglected. In this paper, a phase-amplitude(P-A) model is proposed for a DFIG based on phase and amplitude of the internal voltage. The model structure is much like that of the synchronous generator, and the rotor voltage can manipulate both the amplitude and the phase of the internal voltage.Comparisons have been made between the new P-A model of the DFIG and the synchronous generator model,as well as the asynchronous motor model.The contributions of the new P-A model of the DFIG are discussed and it is demonstrated that the proposed model has better ability in describing power system dynamic phenomena such as voltage dynamics and structural dynamics. Simulation results and a field test validate these contributions. | Hua HUANG Ping JU Xueping PAN Yuqing JIN Xiaoming YUAN Yuan GAO | 2019 | Journal of Modern Power Systems and Clean Energy2019,7,2: | 2 |
| 9 | Effect of carbon and nitrogen ratio control on Artemia growth, water quality, biofloc microbial diversity under high salinity and zero-water exchange culture condition显示文摘Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species. The growth of heterotrophic microorganisms can be stimulated through adding extra carbon, which reduces the nitrogen level in the water and provides microbial protein to the animals. However, most of the studies and practical applications have been conducted in freshwater and marine environment. This paper focused on brine shrimp Artemia that lives in high salinity environment together with other halophilic or halotolerant microorganisms. The effect of carbon supplementation on Artemia growth, water quality, and microbial diversity of biofl ocs was studied in the closed culture condition without any water exchange. The salinity of the culture medium was 100. A 24-d culture trial was conducted through supplementing sucrose at carbon/nitrogen (C/N) ratio of 5, 15, and 30 (Su5, Su15, and Su30), respectively. The culture without adding sucrose was used as a control. Artemia was fed formulated feed at a feeding ration of 60% recommended feeding level. The results showed that sucrose supplementation at higher C/N ratio (15 and 30) signifi cantly improved the Artemia survival, growth and water quality ( P <0.05). Addition of sucrose at C/N ratio of 15 and 30 significantly increased biofloc volume (BFV)( P <0.05). The Illumina MiSeq sequencing analysis showed that supplementing carbon at C/N ratio of 15 had a better total bacterial diversity and richness, and shaped the microbial composition at genera level. This study should provide information for studying the mechanism of biofloc technology and its application in high salinity culture conditions. | WANG Shanyue CUI Xueping XU Ruyi GAO Meirong SUI Liying | 2019 | Journal of Oceanology and Limnology2019,37,5: | 2 |
| 10 | Metal phosphides and borides as the catalytic host of sulfur cathode for lithium–sulfur batteries显示文摘Lithium−sulfur batteries are one of the most competitive high-energy batteries due to their high theoretical energy density of _(2)600 W·h·kg^(−1).However,their commercialization is limited by poor cycle stability mainly due to the low intrinsic electrical conductivity of sulfur and its discharged products(Li_(2)S_(2)/Li_(2)S),the sluggish reaction kinetics of sulfur cathode,and the“shuttle effect”of soluble intermediate lithi-um polysulfides in ether-based electrolyte.To address these challenges,catalytic hosts have recently been introduced in sulfur cathodes to en-hance the conversion of soluble polysulfides to the final solid products and thus prevent the dissolution and loss of active-sulfur material.In this review,we summarize the recent progress on the use of metal phosphides and borides of different dimensions as the catalytic host of sulfur cathodes and demonstrate the catalytic conversion mechanism of sulfur cathodes with the help of metal phosphides and borides for high-en-ergy and long-life lithium-sulfur batteries.Finally,future outlooks are proposed on developing advanced catalytic host materials to improve battery performance. | Rui Gao Zhenyu Wang Sheng Liu Guangjie Shao Xueping Gao | 2022 | International Journal of Minerals,Metallurgy and Materials2022,29,5: | 2 |
| 11 | Photo-assisted Rechargeable Metal Batteries for Energy Conversion and Storage显示文摘Solar cells hold a function of photovoltaic conversion,while rechargeable metal batteries have an advantage of high energy storage.The conventional charge mode of batteries is made based on complete utilization of electric energy.The combination of solar cells and rechargeable metal batteries brings a new opportunity for the development of photo-assisted rechargeable batteries,in which the solar energy can be utilized to partially achieve photo-charging with or without external electrical bias.This review highlights the working mechanism and structure design of photo-assisted rechargeable metal batteries according to the characteristics of rechargeable metal batteries and advantage of the photovoltaic technology.In particular,the recent advances are introduced for photo-assisted rechargeable batteries based on light-weight metal anodes,including metal lithium,metal sodium,and metal zinc.The working features of the integrated devices are also discussed for energy saving under photo-assisted charging mode.Finally,a future outlook is provided for further improving the performance of photoassisted rechargeable metal batteries. | Nanfu Yan Xueping Gao | 2022 | Energy & Environmental Materials2022,5,2: | 2 |
| 12 | Carbon nitride transparent counter electrode prepared by magnetron sputtering for a dye-sensitized solar cell显示文摘Carbon nitride(CN_x) films supported on fluorine-doped tin oxide(FTO) glass are prepared by radio frequency magnetron sputtering, in which the film thicknesses are 90-100 nm, and the element components in the CNX films are in the range of x = 0.15-0.25. The as-prepared CN_x is for the first time used as counter electrode for dye-sensitized solar cells(DSSCs), and show a preparation-temperature dependent electrochemical performance. X-ray photoelectron spectroscopy(XPS) demonstrates that there is a higher proportion of sp^2 C=C and sp^3 C-N hybridized bonds in CN_x-500(the sample treated at 500 ℃) than in CNX-RT(the sample without a heat treatment). It is proposed that the sp^2 C=C and sp^3 C-N hybridized bonds in the CN_x films are helpful for improving the electrocatalytic activities in DSSCs. Meanwhile, Raman spectra also prove that CN_x-500 has a relatively high graphitization level that means an increasing electrical conductivity. This further explains why the sample after the heat treatment has a higher electrochemical performance in DSSCs. In addition, the as-prepared CN_x counter electrodes have a good light transmittance in the visible light region. The results are meaningful for developing low-cost metal-free transparent counter electrodes for DSSCs. | Chaoyu Wu Guoran Li Xueqin Cao Bao Lei Xueping Gao | 2017 | Green Energy & Environment2017,2,3: | 2 |
| 13 | Preparation of porous spherical α-Ni(OH) 2 and enhancement of high-temperature electrochemical performances through yttrium addition 显示文摘 | Ren Junxia Zhou Zhen Gao Xueping | 2006 | Electrochim Acta2006,52,3: | 1 |
| 14 | 显示文摘 | Zhou Zhen Gao Xueping | 2006 | Carbon2006,44,5: | 1 |
| 15 | Fair Efficiency Comparisons of Decoy-State Quantum Key Distribution Protocols显示文摘Taking the schemes based on different formulas of secure key rate as examples, we give a fair comparison between typical protocols under Universal composable(UC) security frame. Through analyzing the relationship of security parameters in post-processing stage and final secure key rate, we achieve the unified quantification between protocols based on Gottesman-Lo-L¨utkenhausPreskill(GLLP) formula and the ones under UC security.Based on the above research, the impact of different sending lengths and secure parameters on secure key rate is investigated. Besides, we analyze the conditions of fair comparison, and for the first time we give a fair comparison between the protocols based on GLLP formula and smooth entropy. By typical efficiency comparisons, we summarize the way for improving secure key rate in the light intensity choice and the single bit error rate estimation method. | LI Hongxin GAO Ming YAN Xueping YAN Bao HAN Yu SHAN Ling MA Zhi | 2018 | Chinese Journal of Electronics2018,27,2: | 1 |
| 16 | An in Vitro Investigation of Acid Etching Treatment Prior to Post and Core Cementation and Teeth Fracture Resistance显示文摘In order to investigate how to enhance the teeth fracture resistance after the post and core treatment, an in vitro study was conducted to measure the fracture resistance of endodontically treated teeth restored with cast post and core with two kinds of surface treatment technology and acid etching preparation on the dentinal surface. Sixty-four recently extracted human single-rooted first premolars were endodontically treated and sectioned approximately 1.5 mm above the cementoenamel junction to remove the coronal portion. Each specimen received a cast post, core build-up and a metal alloy crown restoration. All teeth were randomly divided into the smooth surface post, core repair group, the sand blasting surface post, and core repair group, each group was divided into 10 s, 30 s, 60 s acid corrosion treatment group and control group. In acid test groups, an acid etching solution was applied for 10, 30 and 60 seconds, respectively, to the root canal wall surface. Each specimen was embedded in acrylic resin block and tested in an electronic universal testing machine. Fracture loads results showed that canal acid etching could increase teeth fracture resistance strength both in smooth groups and sandblasting group, and achieved the best effect when acid etching for 30 s. Sand spray treatment on the surface of the cast metal post can improve the flexural strength of the teeth after postcrown restoration. Acid etching on the root canal wall surfaces and sand spray treatment on the surface of the cast metal post can improve the flexural strength of the root after post-crown restoration. Therefore, these two methods could be used to strengthen the tooth fracture resistance, and maintain the long-term therapeutic effect of cast post and core restoration. | 马晓妮 GAO Xueping SUN Baiyu HOU Xiujuan 高旭 徐欣 | 2019 | Journal of Wuhan University of Technology(Materials Science)2019,34,5: | 1 |
| 17 | Free fatty acid receptor 2 promotes cardiomyocyte hypertrophy by activating STAT3 and GATA4显示文摘Free fatty acids(FFAs)play important roles in cardiovascular disease.Studies have shown that it is an important way for FAs to exert biological effects through their own receptors besides directly participating biochemical reaction in body.Free fatty acid receptor 2(FFA2)can be activated by short-chain FAs and is involved in inflammatory reactions and lipid accumulation.Since the known pathological changes caused by FFA2 are also implicated in cardiac hypertrophy,we hypothesized that FFA2 might be pathogenic in cardiac hypertrophy.This paper showed that FFA2 expression significantly increased in cardiac hypertrophy in vivo and in vitro.FFA2 agonist 4-CMTB or TUG-1375 promoted the expression of the hypertrophy markers ANF and BNP and increased cell surface area in vitro,which was further strengthened by FFA2 overexpression,suggesting that FFA2 might contribute to cardiomyocyte hypertrophy.Furthermore,4-CMTB treatment or FFA2 overexpression combined with 4-CMTB treatment elevated the phosphorylation and transcriptional activity of GATA4 and STAT3,which were inhibited by an ERK1/2 inhibitor,and GATA4 and STAT3 knockdown inhibited the elevation of hypertrophy biomarkers in cardiomyocytes treated with 4-CMTB.Taken together,these data indicate that FFA2 can enhance cardiomyocyte hypertrophy by activating STAT3 and GATA4 via ERK1/2,providing a potential new target for therapy. | Hui Gao Kunming Tian Xiaojun Feng Mengqing Yan Chen Gao Yisheng Jiang Chenhao Zhu Huzhe Zhu Xueping Liu Yingfu Peng | 2022 | Food Science and Human Wellness2022,11,2: | 1 |
| 18 | High-Entropy Alloys to Activate the Sulfur Cathode for Lithium-Sulfur Batteries显示文摘Sulfur element possesses an ultrahigh theoretical specific capacity,while the utilization of sulfur in the whole cathode is lower obviously owing to the sluggish kinetics of sulfur and discharged products,limiting the enhancement on energy density of lithium-sulfur batteries.Herein,for the first time,Fe_(0.24)Co_(0.26)Ni_(0.10)Cu_(0.15)Mn_(0.25)high-entropy alloy is introduced as the core catalytic host to activate the electrochemical performance of the sulfur cathode for lithium-sulfur batteries.It is manifested that Fe_(0.24)Co_(0.26)Ni_(0.10)Cu_(0.15)Mn_(0.25)high-entropy alloy nanocrystallites distributed on nitrogen-doped carbon exhibit high electrocatalytic activity toward the conversion of solid sulfur to solid discharged products across soluble intermediate lithium polysulfides.In particular,benefiting from the accelerated kinetics by high-entropy alloy nanocrystallites and synergistic adsorption by nitrogen-doped carbon,the cathode exhibits high reversible capacity of 1079.5 mAh g_(-cathode)^(-1)(high utilization of 89.4%)with the whole cathode as active material,instead of sulfur element.Moreover,under both lean electrolyte(3μmg^(-1))and ultrahigh sulfur loading(27.0 mg cm^(-2))condition,the high discharge capacity of 868.2 mAh g_(-cathode)^(-1)can be still achieved for the sulfur cathode.This strategy opens up a new path to explore catalytic host materials for enhancing the utilization of sulfur in the whole cathode for lithium-sulfur batteries. | Zhenyu Wang Hailun Ge Sheng Liu Guoran Li Xueping Gao | 2023 | Energy & Environmental Materials2023,6,3: | 1 |
| 19 | A promising sol-gel route based on citric acid to synthesize Li3V2(PO4)Jcarbon positive material for lithium ion batteries 显示文摘 | Li Yuzhan Zhou Zhen Gao Xueping | 2007 | Electrochimica Acta2007,52,15: | 1 |
| 20 | Scours of sandy seabed in front of breakwaters under the action of standing waves显示文摘 | Gao Xueping Zao Zidan | 1994 | Journal of Hydraulic Engineering1994,6,: | 1 |