|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Autophagy inhibition enhances apigenin-induced apoptosis in human breast cancer cells显示文摘Apigenin (4',5,7-trihydroxyflavone) is a member of the flavone subclass of flavonoids present in fruits and vegetables. The involvement of autophagy in the apigenin-induced apoptotic death of human breast cancer cells was investigated. Cell proliferation and viability were assessed by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) and clonogenic assays. Flow cytometry, fluorescent staining and Western blot analysis were employed to detect apoptosis and autophagy, and the role of autophagy was assessed using autophagy inhibitors. Apigenin dose- and time-dependently repressed the proliferation and clonogenic survival of the human breast cancer T47D and MDA-MB-231 cell lines. The death of T47D and MDA-MB-231 cells was due to apoptosis associated with increased levels of Caspase3, PARP cleavage and Bax/Bcl-2 ratios. The results from flow cytometry and fluorescent staining also verified the occurrence of apoptosis. In addition, the apigenin-treated cells exhibited autophagy, as characterized by the appearance of autophagosomes under fluorescence microscopy and the accumulation of acidic vesicular organelles (AVOs) by flow cytometry. Furthermore, the results of the Western blot analysis revealed that the level of LC3-II, the processed form of LC3-I, was increased. Treatment with the autophagy inhibitor, 3-methyladenine (3-MA), significantly enhanced the apoptosis induced by apigenin, which was accompanied by an increase in the level of PARP cleavage. Similar results were also confirmed by flow cytometry and fluorescence microscopy. These results indicate that apigenin has apoptosis-and autophagy-inducing effects in breast cancer cells. Autophagy plays a cyto-protective role in apigenin-induced apoptosis, and the combination of apigenin and an autophagy inhibitor may be a promising strategy for breast cancer control. | Xuchen Cao Bowen Liu Wenfeng Cao Weiran Zhang Fei Zhang Hongmeng Zhao Ran Meng Lin Zhang Ruifang Niu Xishan Hao Bin Zhang | 2013 | Chinese Journal of Cancer Research2013，25，2： | 12 |
| 2 | Validation of American Joint Committee on Cancer 8th edition of TNM staging in resected distal pancreatic cancer显示文摘BACKGROUND In order to improve risk stratification and clinical management of the pancreatic ductal adenocarcinoma(PDAC),the American Joint Committee on Cancer(AJCC)has published its eighth edition staging manual.Some major changes have been introduced in the new staging system for both T and N categories.Given the rarity of resectable disease,distal pancreatic cancer is likely underrepresented in the published clinical studies,and how the impact of the staging system actually reflects on to clinical outcomes remain unclear.AIM To validate the AJCC 8th edition of TNM staging in distal PDAC.METHODS A retrospective cohort study was performed in seven academic medical centers in the United States.Clinicopathological prognostic factors associated with progression-free survival(PFS)and overall survival(OS)were evaluated through univariate and multivariate analyses.RESULTS Overall,454 patients were enrolled in the study,and were divided into 2 subgroups:Invasive intraductal papillary mucinous neoplasms(IPMN)(115 cases)and non-IPMN associated adenocarcinoma(339 cases).Compared to invasive IPMN,non-IPMN associated adenocarcinomas are more common in relatively younger patients,have larger tumor size,are more likely to have positive lymph nodes,and are associated with a higher tumor(T)stage and nodal(N)stage,lymphovascular invasion,perineural invasion,tumor recurrence,and a worse PFS and OS.The cohort was predominantly categorized as stage 3 per AJCC 7th edition staging manual,and it’s more evenly distributed based on 8th edition staging manual.T and N staging of both 7th and 8th edition sufficiently stratify PFS and OS in the entire cohort,although dividing into N1 and N2 according to the 8th edition does not show additional stratification.For PDAC arising in IPMN,T staging of the 7th edition and N1/N2 staging of the 8th edition appear to further stratify PFS and OS.For PDAC without an IPMN component,T staging from both versions fails to stratify PFS and OS.CONCLUSION The AJCC 8th edition TNM staging system provides even distribution for the T staging,however,it does not provide better risk stratification than previous staging system for distal pancreatic cancer. | Feng Yin Mohammed Saad Hao Xie Jingmei Lin Christopher R Jackson Bing Ren Cynthia Lawson Dipti M Karamchandani Belen Quereda Bernabeu Wei Jiang Teena Dhir Richard Zheng Christopher W Schultz Dongwei Zhang Courtney L Thomas Xuchen Zhang Jinping Lai Michael Schild Xuefeng Zhang Xiuli Liu | 2020 | World Journal of Gastrointestinal Pharmacology and Therapeutics2020，11，2： | 1 |
| 3 | The Coevolutionary Relationship of Technology, Market and Government Regulation in Telecommunications显示文摘The telecommunications industry has been undergoing tremendous technological changes, and owning to continuous technological advancement, it has maintained sustained prosperity and development. In this paper, the interplay between technology, market and government in telecommunications is discussed briefly, and then we introduce technology and government into the traditional SCP(Structure – Conduct – Performance) paradigm to develop an industry analysis framework called TGM(SCP)(Technology – Government – Market(Structure – Conduct – Performance)). Based on this framework, we present the spiral coevolution model which elaborates on the interaction mechanism of technological innovation with government regulation and market dynamics from the perspective of industry evolution. Our study indicates that the development of the telecommunications industry is the result of the coevolution of technology, government regulation and market forces, and among the three actors, technology is the fundamental driving force. Relative to the 'invisible hand'(market) and 'visible hand'(government), we conceptualize technology as the 'third hand', which fundamentally drives the development of telecommunications industry in coordination with the other two hands. We also provide several policy implications regarding these findings. | Xuchen Lin Ting-Jie Lv Xia Chen | 2018 | China Communications2018，15，8： | 1 |
| 4 | Mapping HⅠ in the NGC 4636 Galaxy Group with FAST显示文摘This paper presents data from a 21 cm H I emission drift scan observation of a field partially covering the NGC 4636 galaxy group with the Five-hundred meter Aperture Radio Telescope(FAST). We construct a pipeline to reduce the data, and use So Fi A for source finding. When not contaminated by Radio Frequency Interference(RFI), the FAST observations are capable of detecting all of the galaxies previously detected by the Arecibo Legacy Fast ALFA(ALFALFA) survey in the same region. Comparing to ALFALFA for the detections in common, the FAST data show consistent integrated spectra when the H I disks are spatially unresolved, and capture more flux when the H I disks are resolved. The FAST data further reveal 10 new detections in the region mutually covered with ALFALFA, and 18 new detections beyond the footprint of ALFALFA. All of the new detections have the matching optical counterparts. For the member galaxies of the NGC 4636 group, the detection limit of FAST is deeper by 0.4 dex in H I mass than that of the ALFALFA data. After correcting for the incompleteness caused by RFI contamination, we show that the H I detection rate of galaxies rises steeply with radius out to the virial radius of the group, and flattens beyond that. We also examine four spatially resolved galaxy systems with potential tidal interaction features in detail. Considering that the data have been taken during the“shared-risk” period before a major source of local RFI was eliminated, the results highlight the power of FAST in detecting extragalactic H I. | Pei Zuo Dong Yang Jing Wang Lister Staveley-Smith Xuchen Lin Bi-Qing For Tobias Westmeier Jie Wang Kristine Spekkens Virginia Kilborn O.Ivy Wong Di Li Karen Lee-Waddell Barbara Catinella Luis C.Ho Barbel Koribalski Bumhyun Lee Ming Zhu | 2022 | Research in Astronomy and Astrophysics2022，22，9： | 0 |
| 5 | 脾血管浸润是胰体尾癌切除术后的不良预后因素显示文摘背景:由于较高的远处转移率,胰体尾癌是致死率最高的肿瘤之一。本研究旨在评估脾血管浸润对于胰体尾癌切除术患者的预后价值。方法:在这项回顾性研究中,我们收集了454例胰体尾癌患者的临床病理资料,通过单因素和多因素分析明确本组患者无进展生存(PFS)和总体生存(OS)的预后影响因素,重点关注脾动/静脉浸润的预后价值。结果:单因素分析结果显示,肿瘤较大、非导管内乳头状黏液瘤(non-IPMN)相关的腺癌、低分化、pT3期、淋巴结转移、脉管浸润、神经浸润、病理学脾静脉浸润、影像学脾静脉浸润者PFS和OS均显著缩短(均P<0.05)。多因素分析证实,non-IPMN相关腺瘤、pT3期、pN1-2期及术后辅助化疗是PFS和OS的独立危险因素;此外,肿瘤较大和影像学脾静脉浸润同时也是PFS的独立预后因素。结论:应当就如何检测和报告胰体尾癌手术标本的脾血管状态,形成一个统一的规范。 | Feng Yin Mohammed Saad Jingmei Lin Christopher R.Jackson Bing Ren Cynthia Lawson Dipti M.Karamchandani Belen Quereda Bernabeu Wei Jiang Teena Dhir Richard Zheng Christopher W.Schultz Dongwei Zhang Courtney L.Thomas Xuchen Zhang Jinping Lai Michael Schild Xuefeng Zhang Hao Xie Xiuli Liu | 2021 | Gastroenterology Report2021，9，2： | 0 |
| 6 | An Interactive Online Platform for Algorithms Teaching显示文摘Learning programming has become an important part of education.However,most students have extreme difficulty learning programming and complex algorithms.This is because programming has a hierarchical logic.Solving complex problems requires students to develop skills in decomposing problems.To this end,this paper describes an effective method to develop an online platform for teaching complex algorithms. | Wenhao Zhou Lin Liu Qiufeng Wang Yusen Wu Xuchen Wang Jia Wang | 2022 | 计算机教育2022，，12： | 0 |
| 7 | Cryptic divergences and repeated hybridizations within the endangered “living fossil” dove tree(Davidia involucrata) revealed by whole genome resequencing显示文摘The identification and understanding of cryptic intraspecific evolutionary units(lineages) are crucial for planning effective conservation strategies aimed at preserving genetic diversity in endangered species.However, the factors driving the evolution and maintenance of these intraspecific lineages in most endangered species remain poorly understood. In this study, we conducted resequencing of 77 individuals from 22 natural populations of Davidia involucrata, a “living fossil” dove tree endemic to central and southwest China. Our analysis revealed the presence of three distinct local lineages within this endangered species, which emerged approximately 3.09 and 0.32 million years ago. These divergence events align well with the geographic and climatic oscillations that occurred across the distributional range.Additionally, we observed frequent hybridization events between the three lineages, resulting in the formation of hybrid populations in their adjacent as well as disjunct regions. These hybridizations likely arose from climate-driven population expansion and/or long-distance gene flow. Furthermore, we identified numerous environment-correlated gene variants across the total and many other genes that exhibited signals of positive evolution during the maintenance of two major local lineages. Our findings shed light on the highly dynamic evolution underlying the remarkably similar phenotype of this endangered species. Importantly, these results not only provide guidance for the development of conservation plans but also enhance our understanding of evolutionary past for this and other endangered species with similar histories. | Yumeng Ren Lushui Zhang Xuchen Yang Hao Lin Yupeng Sang Landi Feng Jianquan Liu Minghui Kang | 2024 | Plant Diversity2024，46，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员