| 1 | Transcriptome Analysis and Genes Function Verification of Root Development of Paeonia suffruticosa under Sandy Loam Cultivation显示文摘Relatively poor in vitro rooting has limited the large-scale commercial production of tree peony.In this study,on the basis of transcriptome sequencing,differentially expressed genes and the associated metabolic pathways were identified in tree peony roots at different stages of root formation under sandy loam cultivation.A total of 31.63 Gb raw data were generated and 120,188 unigenes(mean length of 911.98 bp)were annotated according to six databases(NR,NT,GO,KEGG,COG,and Swiss-Prot).Analyses of the ungerminated root primordium period,induced root primordium period,and root formation period detected 8,232,6,907,and 10,687 differentially expressed genes related to 133,132,and 133 metabolic pathways,respectively.Two significantly differentially expressed genes(Unigene13430_All and CL10096.Contig1_All)were associated with the auxin pathway.The full-length Unigene13430_All coding sequence(843 bp)encoded 280 amino acids,whereas the full-length CL10096.Contig1_All coding sequence(1,470 bp)encoded 489 amino acids.Unigene13430_All and CL10096.Contig1_All were identified as IAA gene family members and were respectively named PsIAA27 and PsARF19.The qRT-PCR analysis and functional verification indicated that the expressions of PsARF19 and PsIAA27 in tree peony seedlings,cuttings and grafted seedlings were significant different.PsARF19 promoted root development,it might be a regulatory gene related to the formation of tree peony roots,while PsIAA27 inhibited lateral root development,and it might be involved in controlling auxin sensitivity during root formation.The results of this study may form the basis of future investigations on the mechanism mediating peony root formation.The transcriptome data will be an excellent resource for researchers interested in characterizing the rooting-related tree peony genes. | Yinglong Song Wenqian Shang Zheng Wang Songlin He Xinya Meng Liyun Shi Yuxiao Shen Dan He Xueyuan Lou Yuke Sun | 2022 | Phyton-International Journal of Experimental Botany2022，91，12： | 0 |
| 2 | LncRNA109897-JrCCR4-JrTLP1b forms a positive feedback loop to regulate walnut resistance against anthracnose caused by Colletotrichum gloeosporioides显示文摘Walnut anthracnose induced by Colletotrichum gloeosporioides is a disastrous disease that severely restricts the development of the walnut industry in China.Long non-coding RNAs(lncRNAs)are involved in adaptive responses to disease,but their roles in the regulation of walnut anthracnose resistance response are not well defined.In this study,transcriptome analysis demonstrated that a C.gloeosporioides-induced lncRNA,lncRNA109897,located upstream from the target gene JrCCR4,upregulated the expression of JrCCR4.JrCCR4 interacted with JrTLP1b and promoted its transcriptional activity.In turn,JrTLP1b induced the transcription of lncRNA109897 to promote its expression.Meanwhile,transient expression in walnut leaves and stable transformation of Arabidopsis thaliana further proved that lncRNA,JrCCR4,and JrTLP1b improve the resistance of C.gloeosporioides.Collectively,these findings provide insights into the mechanism by which the lncRNA109897-JrCCR4-JrTLP1b transcriptional cascade regulates the resistance of walnut to anthracnose. | Rui Zhou Yuhui Dong Changxi Wang Jianning Liu Qiang Liang Xiaoye Meng Xinya Lang Shengyi Xu Wenjun Liu Shuhui Zhang Nan Wang Ke Qiang Yang Hongcheng Fang | 2023 | Horticulture Research2023，10，6： | 0 |
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