| 1 | Effects of dietary supplementation of probiotic,Clostridium butyricum,on growth performance,immune response,intestinal barrier function,and digestive enzyme activity in broiler chickens challenged with Escherichia coli K88显示文摘Background:Colibacillosis caused by enterotoxigenic Escherichia coli(E.coli) results in economic losses in the poultry industry.Antibiotics are usually used to control colibacillosis,however,E.coli has varying degrees of resistance to different antibiotics.Therefore the use of probiotics is becoming accepted as an alternative to antibiotics.In this study,we evaluated the effects of Clostridium butyricum(C.butyricum) on growth performance,immune response,intestinal barrier function,and digestive enzyme activity in broiler chickens challenged with Escherichia coli(E.coif) K88.Methods:The chickens were randomly divided into four treatment groups for 28 days.Negative control treatment(NC) consisted of birds fed a basal diet without E.coli K88 challenge and positive control treatment(PC) consisted of birds fed a basal diet and challenged with E.coli K88.C.butyricum probiotic treatment(CB) consisted of birds fed a diet containing 2×10~7 cfu C butyricum/kg of diet and challenged with E.coli K88.Colistin sulfate antibiotic treatment(CS)consisted of birds fed a diet containing 20 mg colistin sulfate/kg of diet and challenged with E.coli K88.Results:The body weight(BW) and average day gain(ADG) in the broilers of CB group were higher(P< 0.05) than the broilers in the PC group overall except the ADG in the 14-21 d post-challenge.The birds in CB treatment had higher(P < 0.05) concentration of tumor necrosis factor-α(TNF-α) at 3 and 7 d post-challenge,and higher(P < 0.05)concentration of interleukin-4(IL-4) at 14 d post-challenge than those in the PC treatment group.The concentration of serum endotoxin in CB birds was lower(P<0.05) at 21 d post-challenge,and the concentrations of serum diamine oxidase in CB birds were lower(P < 0.05) at 14 and 21 d post-challenge than in PC birds.Birds in CB treatment group had higher(P< 0.05) jejunum villi height than those in PC,NC,or CS treatment at 7,14,and 21 d post-challenge.In comparison to PC birds,the CB birds had lower(P < 0.05) jejunum crypt depth during the whole experiment.The birds in CB or CS treatment group had higher(P < 0.05) activities of amylase and protease at 3,7,and 14 d post-challenge,and higher(P< 0.05) activity of lipase at 3,7 d post-challenge than PC birds.Conclusions:In all,these results indicate that dietary supplementation with C.butyricum promotes immune response,improves intestinal barrier function,and digestive enzyme activities in broiler chickens challenged with E.coli K88.There is no significant difference between the C.butyricum probiotic treatment and the colistin sulfate antibiotic treatment.Therefore,the C.butyricum probiotic may be an alternative to antibiotic for broiler chickens. | Ling Zhang Lingling Zhang Xiu'an Zhan Xinfu Zeng Lin Zhou Guangtian Cao An'guo Chen Caimei Yang | 2016 | Journal of Animal Science and Biotechnology2016，7，1： | 43 |
| 2 | Level of serum neuron-specific enolase and brain damage in children with febrile seizures显示文摘BACKGROUND: Febrile seizure (FS) has good prognosis in the majority of cases. But there is an ongoing debate on the relationship between complicated febrile seizure (CFS) and later development of temporal lobe epilepsy (TLE) due to hippocampal sclerosis. OBJECTIVE: To evaluate the level of serum neuron-specific enolase (S-NSE) of children with simple febrile seizure (SFS) and complicated febrile seizure and compare with children with non-FS respiratory tract infection. DESIGN: Contrast observation. SETTING: Department of Pediatrics and Department of Laboratory, Fujian Provincial Hospital. PARTICIPANTS: Forty-nine patients who were admitted to Department of Pediatrics of Fujian Provincial Hospital from June 2002 to September 2003 with FS were enrolled in this study. There were 28 boys and 21 girls aged from 5 to 72 months. All children were divided into 2 groups based on frequency and duration. Thirty-two children whose FS occurred within 24 hours (lasting shorter than single and twice durations and also shorter than 10 minutes) were regarded as SFS group; meanwhile, 17 children whose FS occurred within 24 hours (lasting longer than single and twice durations and also longer than 10 minutes) were regarded as CFS group. Another 23 patients who were admitted to our hospital with respiratory tract infection in the same period, without the history and positive symptoms/features of neurological dysfunction, were enrolled as control group. There were 13 boys and 10 girls aged from 5 months to 86 months. All parents were told the facts. METHODS: 2 mL venous blood was collected from FS children within 24 hours after the seizures. Meanwhile, 2 mL venous blood was also collected from children in the control group. Level of S-NSE was measured with enzyme immunoassay procedure, and differences among groups were compared with t test. MAIN OUTCOME MEASURES: Level of S-NSE in each group. RESULTS: A total of 49 children in FS group and 23 ones in control group were involved in the final analysis without any loss. Levels of S-NSE were (5.17±1.31) μg/L in SFS group, (5.84±1.62) μg/L in CFS group and (4.98±1.51) μg/L in control group, respectively. There were no significant differences among groups (t =0.498-1.727, P > 0.05). CONCLUSION: FS may be not able to cause a severe brain damage. | Lang Chen Qiaobin Chen Fang Yang Zhi Lin Xinfu Lin Ying Huanc Xin Zheng Yu Lin | 2006 | Neural Regeneration Research2006，1，5： | 1 |
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