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| 1 | Photoprotective Effects of Hydroxychloroqine and TCMs on Human Keratinocytes Damaged from Ultraviolet Irradiation显示文摘Objective: To investigate damage effects of ultraviolet irradiation on eternal keratinocyte-HaCaT cells and to evaluate photo-protective efficiency of hydroxychloroqine and Traditional Chinese Medicines ( epigallocatechingallate[EGCG], baikal skullcap root and szechwan lovge rhizome) on HaCaT ceils damaged by middle wave ultraviolet(UVB) irradiation. Methods: Subconfluent HaCaT cells were sham or UVB irradiated and treated with above TCM agents. The damage degree of HaCaT cells was observed by a light microscop. Cell growth was recorded by cellcount and cellular activity was detected by MTT method. The secretion amount of IL-6 and TNF-α was measured by ELISA. Results: The irradiation damage of HaCaT cells was depended on the irradiated dosages and cellular activity was reduced by 36%-80%, with a maximum decrease over 90% after 72 h. The intervention of the above drugs may increase the cellular activity by 10%-72%. The photo-protective efficiency was more apparent in F_A^G (from 1.19+0.07 to 1.28 + 0.06, P < 0.01 ) than that in hydroxychloroqine ( from 0.43 + 0.04 to 0.96 + 0.04, P < 0.05). The other two tested drugs also showed photo-protective effect( from 0.44 + 0.07 to 1.21 -+ 0.02, P < 0.05). As to cytokine secretion, EGCG could decline the secretion amount of IL-6 and TNF.a apparently. Hydroxychloroqine and baikal skullcap root baikal skullcap root could only reduce the secretion of ID6. The secretion of IL-6 and TNF-α could not be inhibitedby szechwan lovge rhizome. Conclusion : The injury effect of UVB irradiation on cultured keratinocytes is dose-dependent and the tested drugs have photo-protective potency. Inhibition of cytokine secretion may be one of the mechanisms related to reducing the damage effect of UVB irradiation. | LUODan MINWei LINXiang-fei WUDi MIAOXu | 2004 | Journal of Nanjing Medical University2004，18，4： | 10 |
| 2 | Generation of mouse anti-human urate anion exchanger antibody by genetic immunization and its identification显示文摘Background Human urate anion exchanger (hURAT1) as a major urate transporter expressed on renal tubular epithelial cells regulates blood urate level by reabsorbing uric acid. Antibody is an important tool to study hURAT1. This study aimed, by genetic immunization, to produce mouse anti-hURAT1 polyclonal antibody with high throughput and high specificity and to detect the location of hURAT1 in human kidney.Methods Human renal total RNA was isolated and the entire cDNA of hURAT1 was amplified by RT-PCR. The sequence of intracellular high antigenicity fragment (A280 to R349) was chosen by prediction software of protein antigenicity, and its cDNA was amplified from cDNA of hURAT1, and then cloned into pBQAP-TT vector to construct recombinant plasmid pBQAP-TT-hURAT1-210 for genetic immunization. Mice were inoculated with this recombinant plasmid and two other adjuvant plasmids, pCMVi-GMCSF and pCMVi-Flt3L, which helped to enhance the antibody’s generation. After four weeks, the mice were sacrificed to obtain the anti-hURAT1 antibody from serum. The antibody was identified by western blot analysis and immunohistochemistry. At the same time, rabbit anti-hURAT1 antibody was produced by protein immunization. The specificity and efficiency between the rabbit and mouse anti-hURAT1 antibody were compared by western blot analysis and immunohistochemistry. Results The entire cDNA of hURAT1 and cDNA of its intracellular high immunogenic fragment were amplified successfully. Recombinant plasmid pBQAP-TT-hURAT1-210 for genetic immunization was confirmed by restriction digestion and sequencing. Both!the mouse anti-hURAT1 antibody and rabbit anti-hURAT1 antibody recognized 58kD hURAT1 and 64kD glycosylated hURAT1 protein bands in western blot. Immunohistochemically, hURAT1 was located at the brush border membrane of renal proximal tubular cells. In addition, the throughput and specificity of the mouse anti-hURAT1 antibody were higher than those of the rabbit anti-hURAT1 antibody.Conclusion Genetic immunization can generate anti-hURAT1 polyclonal antibody of high throughput and specificity. | XUGuo-shuang WUDi CHENXiang-mei SHISuo-zhu HONGQuan ZHANGPing LUYang | 2005 | Chinese Medical Journal2005，，8： | 7 |
| 3 | 显示文摘 | CAOFang WUDi HEYong etal(曹芳 吴迪 何勇 等) | | 光学学报0，，： | 3 |
| 4 | Effects of Austempering after Hot Deformation on the Mechanical Properties of Hot Rolled Si-Mn TRIP Steel Sheets显示文摘Excellent mechanical properties are obtained by austempering after hot deformation without subsequent heat treatment in the present Si-Mn TRIP steel sheets. Isothermal holding time after finishing rolling has affected the mechanical properties of this steel. The results show that the sample exhibits a good combination of ultimate tensile strength and total elongation when it is held at the bainite transformation temperature after hot deformation. The stability of retained austenite increases with an increase of isothermal holding time, and a further increase in the holding duration results in a decrease of it. The tensile strength, total elongation and strength ductility reach the maximum values (774MPa, 33% and 25542MPa% respectively) for this sort of hot rolled Si-Mn TRIP steel using the optimal technology. | LIZhuang ZHANGPing-li WUDi | 2004 | 材料热处理学报2004，25，5： | 2 |
| 5 | The utilization of the poisonous Eupatorium adenophorum显示文摘 | Zhang Wudi | 1996 | Yunnan Forestry Sci1996，，1： | 1 |
| 6 | Study on the eatery wastewater treatment by biological disk显示文摘 | Zhang Wudi and Wang Haitao | 1996 | Environment Science and Technology(in Chinese)1996，24，2： | 1 |
| 7 | Ovarian tumor in a 12 - year old female with severe hypothyroidism: A case of Van Wyk and Gmmbach syndrome 显示文摘 | Hunold A Alzen G Wudy SA | 2009 | Pediatr Blood Cancer2009，552，5： | 1 |
| 8 | Anaerobic Fermenta- tion for hydrogen production from 11 organic acids 显示文摘 | Li Jianchang Liu Li Zhang Wudi | 2008 | Scientific Research Monthly2008，7，5： | 1 |
| 9 | The utilization of the poisonous Eupatorium adenophorum显示文摘 | Zhang Wudi | 1996 | Yunnan Forestry Sei1996，，1： | 1 |
| 10 | 显示文摘 | WUDi(吴迪) SHENZhen(沈珍) XUEZhao-Li(薛兆历) etal | | ChineseJlnorgChem(WujiHuaxueXuebao)0，，： | 1 |
| 11 | 显示文摘 | WUDi FENGLei ZHANGChuan-qing etal(吴迪 冯雷 张传清 等) | | 红外与毫米波学报0，，： | 1 |
| 12 | Reference range for serum cortisol in well preterm Infants 显示文摘 | Heekmann M Wudy SA Haack D | 1999 | Archives of disease in child- hood1999，81，： | 1 |
| 13 | Electric energy andpower consumption by light-duty plug-in electricvehicles显示文摘 | WuDi Aliprantis D C Gkritza K | 2011 | IEEE Transactions on Power Systems2011，26，2： | 1 |
| 14 | 显示文摘 | WUDi WUHong-xi CAIJing-bo etal(吴迪 吴洪喜 蔡景波 等) | | 红外与毫米波学报0，，： | 1 |
| 15 | Photoin-duced electron transfer from a conducting polymer to buck-mlnsterfullerene显示文摘 | Sariciftci N S Smilowitz L Heeger A J Wudi F | 1992 | Science1992，258，5087： | 1 |
| 16 | Glimepiride Treatment and IGF-I in Adolescents with Type 1 Diabetes:a Prospective, Randomized, Double-blind, Placebo-controlled Study显示文摘 | Wudy S A Hogel J Dollinger B | 2003 | Diabetes Care2003，26，4： | 1 |
| 17 | Polymer blends for selective laser sintering: material and process requirements 显示文摘 | Dietmar Drummer Katrin Wudy Florian Kuhnlein | 2012 | Physics Procedia2012，39，： | 1 |
| 18 | THIN FILM RHEOLOGY AND TRIBOLOGY OF CONFINED POLYMER MELTS - CONTRASTS WITH BULK PROPERTIES显示文摘 | Luengo G Camphell S E Srdanov V I Wudi F Israelachvili J N | | 0，，： | 1 |
| 19 | Polymer blends for selective laser sintering:Material and process requirements显示文摘 | Drummer D Wudy K Kuhnlein F | 2012 | Physics Procedia2012，39，： | 1 |
| 20 | Composition and roles of in- testinal microbiota in children显示文摘 | GUARINO A WUDY A BASILE F | 2012 | J Matern Fetal Neonatal Med2012，25，1： | 1 |
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