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| 1 | Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells显示文摘AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment (ScFv) of anti-tumor monoclonal antibodiesfor fixed whole cells as the carriers of mAb-bindingantigens.ME,ODS: The recombinant phage displaying librariesfor anti-colorectal tumor mAb MC3Ab, MC5Ab andanti-gastric tumor mAb MGD1 was constructed.Panning and screening were carried outby means ofmodified fixation of colorectal and gastric tumor cellsexpressed the mAb-binding antigens. Concordance ofbinding specificity to tumor cells between phageclones and parent antibodies was analyzed. Thephage of positive clones was identified withcompetitive ELISA, and infected by E.coli HB2151 toexpress soluble ScFv.RESULTS:The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60 %, 24 % and 30 %. MC3-ScFv had Mr 32 000 confirmed by Western blot. Thespecificity to antigen had no difference between 4positive recombinant phage antibodies and MC3Ab.CONCLUSION:The modified process of fixing wholetumor cells is efficient, convenient and feasible to panand screen the phage-displaying ScFv of anti-tumormonoclonal antibodies. | Nie, YZ He, FT Li, ZK Wu, KC Cao, YX Chen, BJ Fan, DM | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 2 | P53 codon 72 polymorphism contributes to breast cancer risk: a metaanalysis based on 39 case-control studies(摘要)显示文摘 | Zhang, ZZ Wang, ML Wu, DM Wang, MM Tong, N Tian, YY Zhang, ZD | 2010 | 南京医科大学学报(自然科学版)2010，30，7： | 3 |
| 3 | Cloning and identification of an angiostatic molecule IP-10/crg-2显示文摘AIM To obtain human and murine cDNAsencoding IFN--y inducible protein 10 (lP--10) andcytokine responsive gene--2 (Crg-2).METHODS The encoding genes of lP-10 and Crg2 were amplified by RT--PCR from Cultured humanfibroblast cells and Balb/ c mouse liver treatedby IFN-y and TNF-a, respectively, and clonedinto plasmids of PUC19 and PGEM3Zf(+ ).RESULTS The nucleotide sequences ot theamplified DNA were confirmed by endonucleasesdigestion and sequencing.CONCLUSION Recombinant lP-10/ crg--2 geneclones with 306 hp and 314 hp inserts wereestablished for further research on biologicalactivities and ligands of hiP-10/mCrg--2. | Liu ZG Yang JH An HZ Wang HY He FT Han ZY Han Y Wu HP Xiao B Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 2 |
| 4 | Isolation and char-acterization of human CD34-Lin-hematopoietic stem cells u-sing cell surface markers AC133and CD7显示文摘 | Gallacher L Murdoch B Wu DM | | Blood0，95，9： | 1 |
| 5 | Prevalence and clustering of car- diovascular risk factors among healthy adults in a Chinese popula- tion: the MJ Health Screening Center Study in Taiwan 显示文摘 | Wu DM Pai L Chu NF | 2001 | Int J Obes Relat Metab Disord2001，25，8： | 1 |
| 6 | Reelin, a guidance signal for the regeneration of the entorhino-hippocampal path 显示文摘 | Wu P Li MS Yu DM | 2008 | Brain Res2008，1208，： | 1 |
| 7 | Association be- tween carbamazepine-induced cutaneous adverse drug reactions and the HLA-B * 1502 allele among patients in central China 显示文摘 | Wu XT Hu FY An DM | 2010 | Epilepsy Behav2010，19，3： | 1 |
| 8 | The tracing study of developingentorhino-hippocampal pathway显示文摘 | Deng JB Yu DM Wu P | 2007 | Int J Dev Neurosci2007，25，4： | 1 |
| 9 | EGFR-STAT3 signaling promotes formation of malignant peripheral nerve sheath tumors显示文摘 | Wu J Patmore DM Jousma E | 2013 | Oncogene2013，1，10： | 1 |
| 10 | Urate oxidase:primarystructure and evolutionary implications显示文摘 | WU XW LEE CC MUZNY DM | 1989 | Proc Natl Acad SciUSA1989，86，23： | 1 |
| 11 | The susceptibility of non-fermentative Gram-negative bacilli to cefoperazone and sulbactam compared with other antibacterial agents显示文摘 | Wang F Zhu DM Wu S | 2003 | Int J Antimicrob Agents2003，22，： | 1 |
| 12 | Association between carbamazepine-in- duced cutaneous adverse drug reactions and the HLA-B * 1502 allele among patients in central China显示文摘 | Wu XT Hu FY An DM | 2010 | Epilepsy & Behavior2010，19，3： | 1 |
| 13 | Impact of religious activity on depression and quality of life of chronic peritoneal dialysis patients in Taiwan显示文摘 | Tsai DM Wu KD | 2003 | J Formos Med Assoc2003，102，2： | 1 |
| 14 | Viral load of high-risk human papillomavirus in cervical squamous intraepithelial lesions显示文摘 | Sun CA Liu JF Wu DM | 2002 | Int J Gynaecol Obstet2002，76，1： | 1 |
| 15 | Significance of COX-1, COX-2 and iNOS expression in superficial gastritis, gastric mucosa atypical hyperplasia and gastric carcinoma显示文摘 | Luo YQ Wu KC Sun AH Pan BR Zhang XY Fan DM | 2000 | Chin J Digestion(中华消化杂志)2000，20，4： | 1 |
| 16 | Persistent nausea and anorexia after bone marrow transplantation显示文摘 | Wu D Hockenbery DM Brentnall TA | 1998 | Transplantation1998，66，： | 1 |
| 17 | Viral load of high-risk human Papillomavirus in cervical squamous intraepithelial lesions 显示文摘 | Sun CA Liu J F Wu DM | 2002 | Int J Gynecol Obstet2002，76，1： | 1 |
| 18 | miR-200 enhances mouse breast cancer cell colonization to form distant metastases 显示文摘 | Dykxhoom DM Wu Y Xie H | 2009 | PLoS One2009，4，： | 1 |
| 19 | Association between carbam- azepine-induced cutaneous adverse drug reactions and the HLA- B * 1502 allele among patients in central China 显示文摘 | Wu XT Hu FY An DM | 2010 | Epilepsy Be- hav2010，191，： | 1 |
| 20 | Peritumoral neutrophils link inflammatory response to disease progression by fostering angio-genesis in hepatocellular carcinoma 显示文摘 | Kuang DM Zhao Q Wu Y | 2011 | J Hepatol2011，54，5： | 1 |
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