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| 1 | Effects of propofol on the systolic and diastolic performance of the postischaemic, reperfused myocardium in rabbits 显示文摘 | Leather HA de Wolff MH Wouters PF | 2003 | Eur J Anaesthesiol2003，20，3： | 1 |
| 2 | High-grade acute organ toxicity as a positive prognostic factor in primary radiochemotherapy for anal carci- noma 显示文摘 | Wolff HA Raus I Jung K | 2011 | International Journal of Radiation Oncology Biology Physics2011，79，5： | 1 |
| 3 | The molecular ba- sis of ehemoradiosensitJvity in rectal cancer:implications for personalized therapies显示文摘 | Grade M Wolff HA Gaedeke J | 2012 | Langenbecks Arch Surg2012，397，4： | 1 |
| 4 | The molecular basis of chemoradiosensitivity in rectal cancer: implications for personalized therapies 显示文摘 | Grade M Wolff HA Gaedcke J | 2012 | Langenbecks Arch Surg2012，397，： | 1 |
| 5 | Ionizing radiation regulates cardiac Ca handling via increased ROS and activated CaMKII显示文摘 | Sag CM Wolff HA Neumann K | 2013 | Basic Res Cardiol2013，108，6： | 1 |
| 6 | STAT3 : A Novel Molecular Mediator of Resistance to Chemoradiotherapy 显示文摘 | Spitzner M Ebner R Wolff HA et 02 | 2014 | Cancers (Basel)2014，6，4： | 1 |
| 7 | The molecular basis of chemoradiosensitivity in rectal cancer: implications for personalized therapies 显示文摘 | Grade M Wolff HA Gaedcke J | 2012 | Langenbecks Arch Surg2012，397，： | 1 |
| 8 | Gender affects acute or- gan toxicity during radiochemotherapy for rectal cancer:Long - term results of the German CAO/ARO/AIO -94 phase IM trial显示文摘 | Wolff HA Conradi LC Beissbarth T | 2013 | Ra- diother Onco12013，13，： | 1 |
| 9 | Is early oral feeding safe 'after elective colorectal surgery? a prospective randomized trial显示文摘 | Wolff BG Pemberton JH Heerden HA | 1995 | Ann Surg1995，222，： | 1 |
| 10 | Effect of clevidipine, an ultra-short acting 1,4-dihydropyridine calcium channel blocking drug, on the potency of isoflurane in rats and dogs 显示文摘 | DE WOLFF MH LEATHER HA WOUTERS PF | 2000 | Eur J Anaesthesiol2000，17，8： | 1 |
| 11 | Analysis of chemokine and chemokine receptor expression in squamous cell carcinoma of the head and neck (SCCHN) cell lines 显示文摘 | Wolff HA Rolke D Rave-Frank M | 2011 | Radiat Environ Biophys2011，50，1： | 1 |
| 12 | Effect of clevidipine, an ultra-short acting 1,4-dihydropyridine calcium channel blocking drug, on the potency of isoflurane in rats and dogs显示文摘 | de Wolff MH Leather HA Wouters PF | 2000 | Eur J Anaesthesiol2000，17，8： | 1 |
| 13 | Tuf m RNA rather than 16S r RNA is associated with culturable Staphylococcus aureus显示文摘AIM: To study the presence of various nucleic acids targets of Staphylococcus aureus(S. aureus) during bacterial growth and antibiotic induced killing in relation to viability.METHODS: S. aureus was cultured to log phase and spiked in Todd Hewitt(TH) broth and whole blood of healthy human volunteers. Viability of S. aureus after flucloxacillin treatment(0, 1, 3 and 6 d) was assessed by culture on bloodagar plates. DNA and RNA were isolated from 200 μL. c DNA synthesis was performed by using random primers. The presence of S. aureus DNA, r RNA, and m RNA were determined by real-time polymerase chain reaction of the 16 S r DNA and tuf gene(elongation factor Tu).RESULTS: S. aureus spiked in TH broth without antibiotics grew from day 0-6 and DNA(tuf and 16S), and 16 S r RNA remained detectable during this whole period. During flucloxacillin treatment S. aureus lost viability from day 3 onwards, while the 16 S r RNA-gene and its RNA transcripts remained detectable. DNA andr RNA can be detected in flucloxacillin treated S. aureus cultures that do not further contain culturable bacteria.However, tuf m RNA became undetectable from day 3onwards. Tuf m RNA can only be detected from samples with culturable bacteria. When spiking S. aureus in whole blood instead of broth no bacterial growth was seen, neither in the absence nor in the presence of flucloxacillin. Accordingly, no increase in DNA and RNA levels of both 16 S r DNA and the tuf gene were detected. CONCLUSION: Tuf m RNA expression is associated with culturable S. aureus and might be used to monitor antibiotic effects. | Anne JM Loonen Petra FG Wolffs Maikel de Bresser Maurice Habraken Cathrien A Bruggeman Mirjam HA Hermans Adriaan JC van den Brule | 2015 | World Journal of Clinical Infectious Diseases2015，5，4： | 0 |
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