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| 1 | Hydrogen sutfide protects colon cancer cells from chemopreventative agent β-phenylethyl isothiocvanate induced apoptosis显示文摘AIM: Hydrogen sulfide (H2S) is a prominent gaseous constituent of the gastrointestinal (GI) tract with known cytotoxic properties. Endogenous concentrations of H2S are reported to range between 0.2-3.4 mmol/L in the GI tract of mice and humans. Considering such high levels we speculate that, at non-toxic concentrations, H2S may interact with chemical agents and alter the response of colonic epithelium cells to such compounds. The GI tract is a major site for the absorption of phytochemical constituents such as isothiocyanates, flavonoids, and carotenoids, with each group having a role in the prevention of human diseases such as colon cancer. The chemopreventative properties of the phytochemical agent β-phenyethyl isothiocyanate (PEITC) are well recognized. However, little is currently known about the physiological or biochemical factors present in the GI tract that may influence the biological properties of ITCs. The current study was undertaken to determine the effects of H2S on PEITC mediated apoptosis in colon cancer cells.METHODS: Induction of apoptosis by PEITC in human colon cancer HCT116 cells was assessed using classic apoptotic markers namely SubG1 population analysis,caspase-3 like activity and nuclear fragmentation and condensation coupled with the MTT (3-(4,5-dimethylthiazol2-yl)-2,5-diphenyl tetrasodium bromide) viability assay and LDH leakage.RESULTS: PEITC significantly induced apoptosis in HGT116cells as assessed by SubG1 population formation, nuclear condensation, LDH leakage and caspase-3 activity after 24 h, these data being significant from control groups (P<0.01). In contrast, co-treatment of cells with physiological concentrations of H2S (0.1-1 mmol/L) prevented PEITC mediated apoptosis as assessed using the parameters described.CONCLUSION: PEITC effectively induced cell death in the human adenocarcinoma cell line HCT116 in vitro through classic apoptotic mechanisms. However, in the presence of H2S, apoptosis was abolished. These data suggest that H2S may play a significant role in the response of colonic epithelial cells to beneficial as well as toxic agents present within the GI tract. | Peter Rose Philip K Moore Shen Han Ming Ong Choon Nam Jeffrey S Armstrong Matt Whiteman | 2005 | World Journal of Gastroenterology2005，11，26： | 13 |
| 2 | Effects of natural products on ischemic heart diseases and cardiovascular system显示文摘Ischemic heart diseases remain the leading cause of death in most developed countries as seen over the past quarter-century.Reducing the mortality rate and prevention of myocardial infarction (MI) are of utmost importance.The use of Western Medicinal drugs for the treatment of hypertension,congestive heart filure and post MI are widely accepted.Although some Traditional Chinese Medicines have been used in mainland,Hong Kong,Taiwan of China in clinics and other Chinese communities worldwide for centuries,our nuderstanding of the scientific principles of these herbal drugs is still far from satisfactory.As a result,their wide spread use in patients is limited throughout the world,especially in Western societies,although some of them could be used as complementary medicines in the treatment of Western medicines or tonics to maintain good health.The mechanisms of action and interaction,and the chemicals they contain remain undetermined in most Chinese traditional medicines,and need to be further elucidated using modem biological tools. | Benny KH TAN Matthew WHITEMAN | 2002 | Acta Pharmacologica Sinica2002，23，12： | 8 |
| 3 | Effect of raw material composition on the mineral phases in lime-fluxed iron ore sinter显示文摘 | Hsieh L H Whiteman J A | 1993 | ISIJ International1993，33，4： | 1 |
| 4 | Thiourea and dimethylthiourea inhibit peroxynitrite dependent damage:Nonspecificity as hydroxyl radical seavengers显示文摘 | Whiteman M Halliwell B | 1997 | Free Radical Biology and Medicine1997，22，7： | 1 |
| 5 | Raman lidar system for the measurement of water vapor and aerosols in the earth's atmosphere显示文摘 | WHITEMAN D N MELFI S H FERRARE R A | 1992 | Appl Opt1992，31，16： | 1 |
| 6 | Dietary pat- terns and risk of oesophageal cancers: a population-based case-control study显示文摘 | Ibiebele TI Hughes MC Whiteman DC | 2011 | Br J Nutr2011，7，： | 1 |
| 7 | Characterization of antioxidant and antiglycation properties and isolation of active ingredients from traditional Chinese medicine显示文摘 | Tang S Y Whiteman M Peng Z F | 2004 | Free Radio Biol Med2004，36，12： | 1 |
| 8 | The contribution of the forestry sector to Mrican economics 显示文摘 | WHITEMAN A LEBEDYS A | 2006 | International Forestry Re- view2006，8，1： | 1 |
| 9 | Using a quality improvement process to create measurable improvement in care delivery for acute stroke显示文摘 | Whiteman R Gould L Oczkowski W | 2011 | Healthc Q2011，14，3： | 1 |
| 10 | Characterization of a novel, water-soluble hydrogen sulfide - releasing molecule (GYY4137):unew insights into the biology of hydrogen sulfide显示文摘 | Li L Whiteman M Guan YY | 2008 | Circulation2008，117，18： | 1 |
| 11 | Modern process mineralogy: Two ease studies 显示文摘 | Lotter N O Kormos L J Oliveira J Fragomeni D Whiteman E | 2011 | Minerals Engineering2011，，24： | 1 |
| 12 | The effect of hydrogen sulfide donors on lipopolysaccharide-induced formation of inflammatory mediators in macrophages显示文摘 | Whiteman M Li L Rose P | | 0，，10： | 1 |
| 13 | Displacement-dependent dry friction damping of a beam-like structure显示文摘 | Whiteman W E Ferri A A | 1996 | Journal of Sound and Vibration1996，198，3： | 1 |
| 14 | Recrystallization and Grain Growth in Hot Rolling显示文摘 | Sellars C M Whiteman J A | 1979 | Metal Science1979，34，： | 1 |
| 15 | Recrystallization and Grain Growth in Hot Rolling 显示文摘 | Sellars C M Whiteman J A | 1979 | Metal Science1979，13，34： | 1 |
| 16 | Using a quality improvement process to create measurable improvement in care delivery for acute stroke显示文摘 | Whiteman R Gould L Oczkowski W | | 0，，03： | 1 |
| 17 | Serous ovarian,fallopian tubeand primary peritoneal cancers:a comparative epidemiological analysis显示文摘 | Jordan SJ Green AC Whiteman DC | 2008 | Int J Cancer2008，7，122： | 1 |
| 18 | Recrystallization and grain growth in hot rolling 显示文摘 | Sellars C M Whiteman J A | 1978 | Metal Science1978，13，34： | 1 |
| 19 | A family of difference set 显示文摘 | Whiteman A L | 1962 | Il- linois J Math1962，6，： | 1 |
| 20 | Nature of inclusions in steel weld metals and their influence on formation of acicular ferrite 显示文摘 | Mills A R Thewlis G Whiteman J A | 1999 | Materials Science and Technology1999，3，12： | 1 |
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