|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Development and characterization of a microfluidic glucose sensing system based on an enzymatic microreactor and chemiluminescence detection显示文摘Chemiluminescence detection was developed as an alternative to amperometric detection for glucose analysis in a portable, microfluidics-based continuous glucose monitoring system. Amperometric detection allows easy determination of hydrogen peroxide, a product of the glucose oxidase-catalyzed reaction of glucose with oxygen, by oxidation at a microelectrode. However, (micro)electrodes in direct contact with physiological sample are subject to electrode fouling, which leads to signal drift, decreased reproducibility and shortened detector lifetimes. Moreover, there are a few species present in the body (e.g. ascorbic acid, uric acid) which can undergo oxidation at the same applied potential as hydrogen peroxide. These species can thus interfere with the glucose measurement, reducing detection specificity. The rationale for exploring chemiluminescence as opposed to amperometric detection is thus to attempt to improve the lifetime and reproducibility of glucose analysis for monitoring purposes, while reducing interference caused by other chemicals in the body. The study reported here represents a first step in this direction, namely the realization of a microfluidic device with integrated silicon photodiode for chemiluminescence detection of glucose. This microflow device uses a chaotic mixing approach to perform enzymatic conversion of glucose, followed by reaction of the hydrogen peroxide produced with luminol to produce light at 425 nm. The chemiluminescence reaction is catalyzed by horseradish peroxidase in the presence of iodophenol. The performance of the fabricated chip was characterized to establish optimal reaction conditions with respect to sample and reagent flow rates, pH, and concentrations. A linear calibration curve was obtained for current response as a function of glucose concentration in the clinically relevant range between 2 and 10 mM, with a sensitivity of 39 pA/mM (R = 0.9963, one device, n = 3) and a limit of detection of 230 μM (S/N = 3). | MOON B.-U. de VRIES M.G. WESTERINK B.H.C. VERPOORTE E. | 2012 | Science China Chemistry2012，55，4： | 2 |
| 2 | Immune responses to pneumococcal vaccines in children and adults: rationale for age- specific vaccination 显示文摘 | Westerink MA Schroeder HW Jr Nahm MH | 2012 | Aging Dis2012，3，1： | 1 |
| 3 | Cytichrome P450 enzyme levels in HepG2 cells and cryopreserved primary human hepatocytes and their induction in HepG2 cells 显示文摘 | Westerink W M Schoonen W G | 2007 | Toxicol In Vitro2007，21，8： | 1 |
| 4 | The significance of extracellular calcium for the release of dopamine,acetylcholine and amino acids in conscious rats,evaluated by brain microdialysis显示文摘 | WESTERINK B H HOFSTEEDE H M DAMSMA G | 1988 | Naunyn Schmiedebergs Arch Pharmacol1988，337，4： | 1 |
| 5 | Novel vaccine strategies to T-independent antigens 显示文摘 | Lesinski GB Westerink MA | 2001 | J Microbiol Methods2001，47，2： | 1 |
| 6 | Adaptive Channel Error Protection of Subband Encoded Images显示文摘 | Westerink P H Wever J H | 1993 | IEEE Trans Communications1993，41，： | 1 |
| 7 | Continuous,discontinuous and coupled discontinuous-continuous Galerkin finite element methods for the shallow water equations显示文摘 | DAWSONL C WESTERINK J J FEYEN J C | 2006 | Int J Numer Methods in Fluids2006，52，： | 1 |
| 8 | Modulation of human ct 4显示文摘 | Hendriks HS van Kleef RG Westerink RH | 2012 | Toxicol Lett2012，213，2： | 1 |
| 9 | Cytochrome P450 enzyme levels in HepG2 cells and cryopreserved pri- mary human hepatocytes and their induction in HepGz cells显示文摘 | Westerink WM Schoonen WG | 2007 | Toxicol in Vitro2007，21，8： | 1 |
| 10 | Phase II enzyme levels in HepG2 cells and cryopreserved primary hu man hepatocytes and their induction in HepG2 cells 显示文摘 | Westerink WM Schoonen WG | 2007 | Toxicology in Vitro2007，21，8： | 1 |
| 11 | Brain microdialysis and its application for the study of animal behavior显示文摘 | Westerink BHC | 1995 | Behavioural brain research1995，70，2： | 1 |
| 12 | Liquid crystalline solutions of cellulose in phosphoric acid 显示文摘 | B Boerstoel H Maatman H Westerink J B | 2001 | Polymer2001，42，17： | 1 |
| 13 | High-throughput screening for analysis of in vitro toxicity 显示文摘 | Schoonen WG Westerink WM Horbach GJ | 2009 | EXS2009，99，： | 1 |
| 14 | Effect of statin therapy on incident type 2 diabetes mellitus in patients with clinically manifest vascular disease显示文摘 | van de Woestijne AP van der Graaf Y Westerink J | 2015 | Am J Cardiol2015，115，4： | 1 |
| 15 | Translating neu- robehavioural endpoints of developmental neurotoxicity tests into in vitro assays and readouts 显示文摘 | van Thriel C Westerink R Beste C | 2012 | Neurotoxicolo- gy2012，33，4： | 1 |
| 16 | Neurotoxicity of brominated flame retardants : (in) direct effects of parent and hydroxylated polybrominated diphenyl ethers on the (developing) nervous system 显示文摘 | Dingemans M M L van den Berg M Westerink R H S | 2011 | Environmental Health Perspectives2011，119，7： | 1 |
| 17 | Aspects of nonlinear simulations using shallow water models based on the wave continuity equation显示文摘 | Kolar R L Westerink J J CanteEn M E | 1994 | Computers and Fluids1994，23，3： | 1 |
| 18 | A multidimensional evaluation of the perceptual quality of television sets显示文摘 | Teunissen K Westerink J | 1996 | Journal of SMPTE1996，105，31： | 1 |
| 19 | Hydroxylation Increases the Neurotoxic Potential of BDE-47 to Affect Exocytosis and Calcium Homeostasis in PC12 Cells显示文摘 | Dingemans Milou M L Groot Aart de Kleef Regina G D M van Bergman ?ke Berg Martin van den Vijverberg Henk P M Westerink Remco H S | 2008 | Environmental Health Perspectives2008，，5： | 1 |
| 20 | High- throughput screening for analysis of in vitro toxicity 显示文摘 | Schoonen WG Westerink WM Horbach GJ | 2009 | EXS2009，1，99： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员