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| 1 | Down.regulation of E.cadherin enhances prostate cancer chemoresistance via Notch signaling显示文摘Background:The chemoresistance of prostate cancer(PCa)is invariably associated with the aggressiveness and metastasis of this disease.New emerging evidence indicates that the epithelial-to-mesenchymal transition(EMT)may play pivotal roles in the development of chemoresistance and metastasis.As a hallmark of EMT,E-cadherin is suggested to be a key marker in the development of chemoresistance.However,the molecular mechanisms underlying PCa chemoresistance remain unclear.The current study aimed to explore the association between EMT and chemoresistance in PCa as well as whether changing the expression of E-cadherin would affect PCa chemoresistance.Methods:Parental PC3 and DU145 cells and their chemoresistant PC3-Tx R and DU145-Tx R cells were analyzed.PC3-Tx R and DU145-Tx R cells were transfected with E-cadherin-expressing lentivirus to overexpress E-cadherin;PC3 and DU145 cells were transfected with small interfering RNA to silence E-cadherin.Changes of EMT phenotype-related markers and signaling pathways were assessed by Western blotting and quantitative real-time polymerase chain reaction.Tumor cell migration,invasion,and colony formation were then evaluated by wound healing,transwell,and colony formation assays,respectively.The drug sensitivity was evaluated using MTS assay.Results:Chemoresistant PC3-Tx R and DU145-Tx R cells exhibited an invasive and metastatic phenotype that associated with EMT,including the down-regulation of E-cadherin and up-regulation of Vimentin,Snail,and N-cadherin,comparing with that of parental PC3 and DU145 cells.When E-cadherin was overexpressed in PC3-Tx R and DU145-Tx R cells,the expression of Vimentin and Claudin-1 was down-regulated,and tumor cell migration and invasion were inhibited.In particular,the sensitivity to paclitaxel was reactivated in E-cadherin-overexpressing PC3-Tx R and DU145-Tx R cells.When E-cadherin expression was silenced in parental PC3 and DU145 cells,the expression of Vimentin and Snail was up-regulated,and,particularly,the sensitivity to paclitaxel was decreased.Interestingly,Notch-1 expression was up-regulated in PC3-Tx R and DU145-Tx R cells,whereas the E-cadherin expression was down-regulated in these cells comparing with their parental cells.The use ofγ-secretase inhibitor,a Notch signaling pathway inhibitor,significantly increased the sensitivity of chemoresistant cells to paclitaxel.Conclusion:The down-regulation of E-cadherin enhances PCa chemoresistance via Notch signaling,and inhibiting the Notch signaling pathway may reverse PCa chemoresistance. | Wenchu Wang Lihui Wang Atsushi Mizokami Junlin Shi Chunlin Zou Jinlu Dai Evan T. Keller Yi Lu Jian Zhang | 2017 | Chinese Journal of Cancer2017，36，3： | 16 |
| 2 | Study on hydraulic performance of drip emitters by computational fluid dynamics显示文摘 | Wei Qingsong Shi Yusheng Dong Wenchu | 2006 | Agricultural Water Management2006，84，12： | 1 |
| 3 | Mitochondrial temperature-responsive drug delivery reverses drug resistance in lung cancer显示文摘Reversal of cancer drug resistance remains a critical challenge in chemotherapy.Mitochondria-targeted drug delivery has been suggested to mitigate drug resistance in cancer.To overcome the intrinsic limitations in conventional mitochondrial targeting strategies,we develop mitochondrial temperature-responsive drug delivery to reverse doxorubicin(DOX)resistance in lung cancer.Results demonstrate that the thermoresponsive nanocarrier can prevent DOX efflux and facilitate DOX accumulation and mitochondrial targeting in DOX-resistant tumors.As a consequence,thermoresponsive nanocarrier enhances the cytotoxicity of DOX and reverses the drug resistance in tumor-bearing mice.This work represents the first example of mitochondrial temperature-responsive drug delivery for reversing cancer drug resistance. | Lifo Ruan Jun Chen Chuanchao Du Huiru Lu Jiayu Zhang Xiaomeng Cai Rui Dou Wenchu Lin Zhifang Chai Guangjun Nie Yi Hu | 2022 | Bioactive Materials2022，7，7： | 1 |
| 4 | Study on hydraulic performance of drip emitters by computational fluid dynamics显示文摘 | Qingsong Wei Yusheng Shi Wenchu Dong Gang Lu Shuhuai Huang | 2006 | Agricultural Water Management2006，，1： | 1 |
| 5 | Study on hydraulic performance of drip emitters by computational fluid dynamics 显示文摘 | Qingsong Wei Yusheng Shi Wenchu Dong | 2006 | Agricultural Water Management2006，，84： | 1 |
| 6 | E-cadherin表达下调激活Notch信号通路增强前列腺癌化疗耐药的机制研究显示文摘背景与目的前列腺癌(prostate cancer,PCa)化疗耐药与其侵袭性和转移性密切相关。最新研究显示上皮细胞–间充质转化(epithelial-to-mesenchymal transition,EMT)可能在前列腺癌化疗耐药和转移中发挥重要作用。因此,作为EMT的标志物,E-cadherin被认为有可能与肿瘤化疗耐药有重要关系。然而,PCa化疗耐药的分子机制尚不清楚。本研究旨在探讨EMT与PCa化疗耐药的关系,以及E-cadherin表达变化是否影响PCa化疗耐药。方法以亲代PC3和DU145细胞及其化疗耐药PC3-TxR和DU145-TxR细胞作为研究对象。用E-cadherin过表达的慢病毒感染PC3-TxR和DU145-TxR细胞,使E-cadherin过表达;同时,用小干扰RNA转染PC3和DU145细胞,使E-cadherin表达沉默。采用蛋白免疫印迹和实时定量聚合酶链反应分析EMT相关标志物及信号通路的变化。分别通过划痕、transwell和克隆形成实验检测肿瘤细胞的迁移、侵袭和克隆形成能力。采用MTS法检测细胞对紫杉醇的药物敏感性。结果与亲代PC3和DU145细胞相比,化疗耐药PC3-TxR和DU145-TxR细胞表现出EMT相关的标志物表达改变,包括E-cadherin表达下调和Vimentin、Snail与N-cadherin表达上调。当E-cadherin在PC3-TxR和DU-145-TxR细胞中过表达时,Vimentin和Caldun-1表达下调,肿瘤细胞的迁移和侵袭受到抑制。特别是E-cadherin过表达以后,PC3-TxR和DU145-TxR细胞对紫杉醇的敏感性被重新激活。并且,在亲代PC3和DU145细胞中沉默E-cadherin表达后,Vimentin和Snail表达上调,对紫杉醇的敏感性降低。有趣的是,与亲代PC3和DU145细胞相比,PC3-TxR和DU145-TxR细胞中Notch-1表达上调,而E-cadherin在这些细胞中的表达下调。我们的研究还显示,Notch信号通路抑制剂γ-分泌酶抑制剂可显著增加化疗耐药细胞对紫杉醇的敏感性。结论 E-cadherin下调可以激活Notch信号通路,从而增强PCa化疗耐药,抑制Notch信号通路可能逆转PCa化疗耐药。 | Wenchu Wang Lihui Wang Atsushi Mizokami Junlin Shi Chunlin Zou Jinlu Dai Evan T.Keller Yi Lu Jian Zhang | 2019 | 癌症2019，38，3： | 1 |
| 7 | Study on hydraulic performance of drip emitters by computational fluid dynamics显示文摘 | Wei Qingsong Shi Yusheng Dong Wenchu | 2006 | Agricultural Water Management2006，84，12： | 1 |
| 8 | Study on hydraulic performance of drip emitters by computational fluid dynamics显示文摘 | Qingsong Wei Yusheng Shi Wenchu Dong | 2006 | Agricultural Water Management2006，，84： | 1 |
| 9 | Inactivation and mechanisms of chlorine dioxide on Nosema bombycis显示文摘 | Zhengyong Wang Fupin Liao Jianrong Lin Wenchu Li Yangsheng Zhong Peichan Tan Ziran Huang | 2009 | Journal of Invertebrate Pathology2009，，2： | 1 |
| 10 | Study on hydraulic performance of drip emitters by computational fluid dynamics 显示文摘 | Wei Qingsong Shi Yusheng Dong Wenchu | 2006 | Agricultural Water Management2006，84，12： | 1 |
| 11 | Advanced methods to develop drip emitters with new channel types显示文摘 | Wei Qingsong Shi Yusheng Dong Wenchu | 2006 | Applied Engineering in Agriculture2006，22，2： | 1 |
| 12 | Advanced methods to develop drip emitters with new channel types 显示文摘 | Wei Qingsong Shi Yusheng Dong Wenchu | 2006 | Applied Engineering in Agriculture2006，22，2： | 1 |
| 13 | Advanced methods to' develop drip emitters with new channel types显示文摘 | Qingsong Wei Yusheng Shi Wenchu Dong | 2006 | American gociety of Agricultural and Bio- logical Engineer2006，22，2： | 1 |
| 14 | Study on hydraulic performance of drip emitters by computational fluid dynamics 显示文摘 | Wei Qingsong Shi Yusheng Dong Wenchu | 2006 | Agricultural Water Management2006，84，12： | 1 |
| 15 | Study on hydraulic performance of drip emitters by computational fluid dynamics显示文摘 | Qingsong Wei Yusheng Shi Wenchu Dong | 2006 | Agricultural Water Management2006，84，12： | 1 |
| 16 | Advanced methods to develop drip emitters with new channel types 显示文摘 | Wei Qingsong Shi Yusheng Dong Wenchu | 2006 | American Society of Agricultural and Biological Engineer2006，22，2： | 1 |
| 17 | Study on hydraulic performance of drip emitters by computational fluid dynamics 显示文摘 | Wei Qingsong Shi Yusheng Dong Wenchu | 2006 | Agricultural Water Management2006，84，12： | 1 |
| 18 | Study on hydraulic performance of drip emitters by computational fluid dynamics 显示文摘 | Wei Qingsong Shi Yusheng Dong Wenchu | 2006 | Agricultural Water Management2006，84，12： | 1 |
| 19 | On the Understanding of Beauty by Keats显示文摘尽管当下济慈研究的重心是济慈同其所处时代的历史联系,但济慈对美的追求是一个未被否认也无法被否认的事实。济慈从整体性角度肯定美的永恒,又从个体存在的层面哀痛美的非永恒性。在济慈眼里,美既来自于人与现实的'遭遇'也来自于人的想象;美是充满活力与能量的人的生活与生命。济慈承认生命内含着自我毁灭性,但生命的真正意义在于超越毁灭;美正是生命的超越性表现。济慈对美的理解是其丰富的诗学思想的基础,他的一些极具创见的诗学观念如'消极能力说'、'非身份性认同'等均是从其基本美学理念中生发出来的. | Zhang Wenchu Hunan Normal University | 2007 | Comparative Literature:East & West2007，，1： | 0 |
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