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| 1 | Differences in biological features of gastric dysplasia, indefinite dysplasia, reactive hyperplasia and discriminant analysis of these lesions显示文摘AIM: To investigate the differences in biological features of gastric dysplasia (Dys), indefinite dysplasia (IDys) and reactive hyperplasia (RH) by studying the biomarker alterations in cell proliferation, cell differentiation, cell cycle control and the expression of house-keeping genes, and further to search for markers which could be used in guiding the pathological diagnosis of three lesions. METHODS: Expressions of MUC5AC, MUC6, adenomatous polyposis coli (APC), p53, Ki-67, proliferation cell nuclear antigen (PCNA) and EGFR were studied by immunohistochemistry with a standard Envision technique in formalinfixed and paraffin-embedded specimens from 43 RH, 35 IDys, 35 Dys and 36 intestinal type gastric carcinomas (IGC). In addition, Bayes discriminant analysis was used to investigate the value of markers studied in differential diagnosis of RH, IDys, Dys and IGC. RESULTS: The MUC5AC and MUC6 antigen expressions in RH, IDys, Dys and IGC decreased gradually (MUC5AC:86.04%, 77.14%, 28.57%, 6.67%; MUC6: 65.15%, 54.29%, 20.00%, 25.00%, respectively). The expressions of the two markers had no significant difference between RH and IDys, but were all significantly higher than those ofthe other two lesions (MUC5AC: x2 = 27.607, 38.027 and 17.33, 26.092; MUC6: x2= 16.54, 12.665 and 9.282, 6.737, P<0.01). There was no significant differencebetween RH and IDys, Dys and IGC in MUC6 expression. The APC gene expression in the four lesions had a similar decreasing tendency (RH 69.76%, IDys 68.57%, Dys39.39%, IGC 22.86%), and it was significantly higher in the first two lesions than in the last two (x2 = 7.011,16.995 and 14.737, 19.817, P<0.05). The p53 expressionin RH, IDys, Dys and IGC was 6.98%, 20%, 57.14% and 50%, respectively. There was no significant differencebetween RH and IDys or Dys and IGC, but the p53 expression in RH and IDys was significantly lower than that in Dys and IGC (x2 = 7.011, 16.995 and 14.737, 19.817, P<0.01).The Ki-67 label index was significantly different among four lesions (RH: 0.298±8.92%, IDys: 0.358±9.25%,Dys: 0.498±9.03%, IGC: 0.620±10.8%, P<0.001). Positive immunostaining of PCNA was though observed in all specimens, significant differences were detected among four lesions (F= 95.318, P<0.01). In addition, we used Bayes discriminant analysis to investigate molecular pathological classification of the lesions, and obtained the best result with the combination of MUC5AC, Ki-67 and PCNA. The overall rate of correct classification was67.4% (RH), 68.6% (IDys), 70.6% (Dys) and 84.8% (IGC), respectively.CONCLUSION: Dys has neoplastic biological characteristics, while RH and IDys display hyperplastic characteristics. MUC5AC and proliferation-related biomarkers (Ki-67, PCNA) are more specific in distinguishing Dys from RH and IDys. | BinDong Yu-QuanXie KeChen TaoWang WeiTang Wei-ChengYou Ji-YouLi | 2005 | World Journal of Gastroenterology2005，11，23： | 7 |
| 2 | Post-transcriptional Gene Silencing Induced by Short Interfering RNAs in Cultured Transgenic Plant Cells显示文摘Short interfering RNA (siRNA) is widely used for studying post-transcriptional gene silencing and holds great promise as a tool for both identifying function of novel genes and validating drug targets. Two siRNA fragments (siRNA-a and -b), which were designed against di?erent speci?c areas of coding region of the same target green ?uorescent protein (GFP) gene, were used to silence GFP expression in cultured gfp transgenic cells of rice (Oryza sativa L.; OS), cotton (Gossypium hirsutum L.; GH), Fraser ?r [Abies fraseri (Pursh) Poir; AF], and Virginia pine (Pinus virginiana Mill.; PV). Di?erential gene silencing was observed in the bom- barded transgenic cells between two siRNAs, and these results were consistent with the inactivation of GFP con?rmed by laser scanning microscopy, Northern blot, and siRNA analysis in tested transgenic cell cultures. These data suggest that siRNA-mediated gene inactivation can be the siRNA speci?c in di?erent plant species. These results indicate that siRNA is a highly speci?c tool for targeted gene knockdown and for establishing siRNA-mediated gene silencing, which could be a reliable approach for large-scale screening of gene function and drug target validation. | WeiTang VanessaSamuels NickiWhitley NicoleBloom TinyaDeLaGarza RonaldJ.Newton | 2004 | Genomics, Proteomics & Bioinformatics2004，2，2： | 4 |
| 3 | Clinical and molecular insights into the hepatocellular carcinoma tumour marker des‐γ‐carboxyprothrombin显示文摘 | YoshinoriInagaki WeiTang MasatoshiMakuuchi KiyoshiHasegawa YasuhikoSugawara NorihiroKokudo | 2010 | Liver International2010，，1： | 1 |
| 4 | Morphogenesis and crystallization of Bi2 S3 nanostructures by an ionic liquid assisted templating route : synthesis, formation mechanism and properties 显示文摘 | Jiang jie Yu Shuhong Yao Weitang | 2005 | Chem Mater2005，17，24： | 1 |
| 5 | Architectural control synthese of CdS and CdSe nanoflowers, branched nanowires and nanotrees via a solvothermal approach in a mixed solution and photocatalytic property显示文摘 | Yao Weitang Yu Shuhong Liu Shujuan | 2006 | J Phys Chem C2006，110，11： | 1 |
| 6 | Functional Genomics of Wood Quality and Properties显示文摘Genomics promises to enrich the investigations of biology and biochemistry. Current advancements in genomics have major implications for genetic improvement in animals, plants, and microorganisms, and for our understanding of cell growth, development, differentiation, and communication. Significant progress has been made in the understanding of plant genomics in recent years, and the area continues to progress rapidly. Functional genomics offers enormous potential to tree improvement and the understanding of gene expression in this area of science worldwide.In this review we focus on functional genomics of wood quality and properties in trees, mainly based on progresses made in genomics study of Pinus and Populus.The aims of this review are to summarize the current status of functional genomics including: (1) Gene discovery; (2) EST and genomic sequencing; (3) From EST to functional genomics; (4) Approaches to functional analysis; (5) Engineering lignin biosynthesis; (6) Modification of cell wall biogenesis; and (7) Molecular modelling.Functional genomics has been greatly invested worldwide and will be important in identifying candidate genes whose function is critical to all aspects of plant growth, development, differentiation, and defense. Forest biotechnology industry will significantly benefit from the advent of functional genomics of wood quality and properties. | WeiTang XiaoyanLuo AaronNelsont HilaryCollver KatherineKinken | 2003 | Genomics, Proteomics & Bioinformatics2003，1，4： | 1 |
| 7 | Hierarchical porous nitrogen,oxygen,and phosphorus ternary doped hollow biomass carbon spheres for high-speed and long-life potassium storage显示文摘Limited lithium resources have promoted the exploration of new battery technologies.Among them,potassium-ion batteries are considered as promising alternatives.At present,commercial graphite and other carbon-based materials have shown good prospects as anodes for potassium-ion batteries.However,the volume expansion and structural collapse caused by periodic K+insertion/extraction have severely restricted further development and application of potassium-ion batteries.A hollow biomass carbon ball(NOP-PB)ternarily doped with N,O,and P was synthesized and used as the negative electrode of a potassium-ion battery.X-ray photoelectron spectroscopy,Fourier‐transform infrared spectroscopy,and transmission electron microscopy confirmed that the hollow biomass carbon spheres were successfully doped with N,O,and P.Further analysis proved that N,O,and P ternary doping expands the interlayer distance of the graphite surface and introduces more defect sites.DFT calculations simultaneously proved that the K adsorption energy of the doped structure is greatly improved.The solid hollow hierarchical porous structure buffers the volume expansion of the potassium insertion process,maintains the original structure after a long cycle and promotes the transfer of potassium ions and electrons.Therefore,the NOP‐PB negative electrode shows extremely enhanced electrochemical performance,including high specific capacity,excellent long‐term stability,and good rate stability. | Mengmeng Yang Qingquan Kong Wei Feng Weitang Yao Qingyuan Wang | 2022 | Carbon Energy2022，4，1： | 1 |
| 8 | 显示文摘 | Zhang Qian Yao Weitang Chen Xianyu | 2007 | Crystal Growth & Design2007，7，8： | 1 |
| 9 | Fumigation of sweet cherries with thymol and acetic acid to reduce post harvest brown rot and blue mold rot 显示文摘 | Chu Chunlung Liu Weitang Zhou Ting | 2001 | Fruits2001，56，2： | 1 |
| 10 | Comprehensive evaluation of combining CFD simulation and entropy weight to predict natural ventilation strategy in a sliding cover solar greenhouse显示文摘This study proposed a comprehensive evaluation system to incorporate the contribution of both numerical simulation and statistical decision theory in ventilation performance assessment.A high-resolution model based on the finite volume approach was established to analyze the influence of rotation angles(i.e.,side vent flip angle and roof vent flip angle)of the rack-and-pinion ventilated structure on the greenhouse microclimate.The water circulating system and tomato seeding canopies were considered.Heat removal efficiency and mean age of air were employed as quantitative attributes to reflect the internal thermal environment and the airflow organization in the sliding cover solar greenhouse.The simulation model was verified with the temperature profile measured and the average relative error was 1.74%.The results demonstrate that the rotating angles of ventilation schemes have a substantial impact on the microclimate and inhomogeneity of the tomato seeding canopies.The results suggest the average velocity and its inhomogeneity are the crucial predictors,and their entropy weight values are 0.231 and 0.218,respectively.The relative degree of membership of the side vent flip angle of 45°is 36%and 97%higher than that of the side vent flip angle of 35°and the side vent flip angle of 25°,respectively.This study can provide a reference to evaluate the ventilated strategies of the sliding cover solar greenhouse for the regional and central government. | He Li Dong Ji Xi Hu Tian Xie Weitang Song Subo Tian | 2021 | International Journal of Agricultural and Biological Engineering2021，14，6： | 1 |
| 11 | Complex wurtzite ZnSe microspheres with high hierarchy and their optical properties 显示文摘 | Weitang Yao Shuhong Yu Jun Jiang | 2006 | Chem Eur J2006，12，： | 1 |
| 12 | Architectural control syntheses of CdS and CdSe nanoflowers, branched nanowires, and nanotrees via a solvothermal approach in a mixed solution and their photocatalytie property显示文摘 | Weitang Yao Shuhong Yu Shujuan Liu | 2006 | J Phys Che B2006，110，11： | 1 |
| 13 | Fumigation of sweet cherries with thymol and acetic acid to reduce post harvest brown rot and blue mold rot显示文摘 | CHU Chunltmg LIU Weitang ZHOU Ting | 2001 | Fruits2001，56，2： | 1 |
| 14 | Control of postharvest gray mold rot of modified atmosphere packaged sweet cherries by fumigation with thymol and acetic acid显示文摘 | CHU Chunlung LIU Weitang ZHOU Ting | 1999 | Can J Plant Sci1999，79，： | 1 |
| 15 | Fumigation of sweet cherries with thymol and acetic acid to reduce post harvest brown rot and blue mold rot显示文摘 | CHU C L LIU Weitang ZHOU Ting | | 0，，： | 1 |
| 16 | Thymol and acetic acid vapors reduce post harvest brown rot of apricot and plums显示文摘 | LIU Weitang CHU Chunlung ZHOU Ting | 2002 | Hort Sci2002，37，： | 1 |
| 17 | Thymol and acetic acid vapors reduce post harvest brown rot of apricot and plums显示文摘 | LIU Weitang CHU C L ZHOU Ting | | 0，，： | 1 |
| 18 | Identification of liver metastasis-associated genes in human colon carcinoma by mRNA profiling显示文摘Objective: Liver metastasis,which contributes substantially to high mortality,is the most common recurrent mode of colon carcinoma.Thus,it is necessary to identify genes implicated in metastatic colonization of the liver in colon carcinoma.Methods: We compared mRNA profiling in 18 normal colon mucosa(N),20 primary tumors(T) and 19 liver metastases(M) samples from the dataset GSE49355 and GSE62321 of Gene Expression Omnibus(GEO) database.Gene ontology(GO) and pathways of the identified genes were analyzed.Co-expression network and proteinprotein interaction(PPI) network were employed to identify the interaction relationship.Survival analyses based on The Cancer Genome Atlas(TCGA) database were used to further screening.Then,the candidate genes were validated by our data.Results: We identified 22 specific genes related to liver metastasis and they were strongly associated with cell migration,adhesion,proliferation and immune response.Simultaneously,the results showed that C-X-C motif chemokine ligand 14(CXCL14) might be a favorable prediction factor for survival of patients with colon carcinoma.Importantly,our validated data further suggested that lower CXCL14 represented poorer outcome and contributed to metastasis.Gene set enrichment analysis(GSEA) showed that CXCL14 was negatively related to the regulation of stem cell proliferation and epithelial to mesenchymal transition(EMT).Conclusions: CXCL14 was identified as a crucial anti-metastasis regulator of colon carcinoma for the first time,and might provide novel therapeutic strategies for colon carcinoma patients to improve prognosis and prevent metastasis. | Jianling Liu Dan Wang Chaoqi Zhang Zhen Zhang Xinfeng Chen Jingyao Lian Jinbo Liu Guixian Wang Weitang Yuan Zhenqiang Sun Weijia Wang Mengjia Song Yaping Wang Qian Wu Ling Cao Dong Wang Yi Zhang | 2018 | Chinese Journal of Cancer Research2018，30，6： | 1 |
| 19 | A fourth locus for hereditary hemorrhagic telangiectasia maps to chromosome 7显示文摘 | PinarBayrak‐Toydemir JamieMcDonald NurtenAkarsu Reha M.Toydemir FernandaCalderon TimurTuncali WeiTang FranklinMiller RongMao | 2006 | Genet2006，，20： | 1 |
| 20 | Current Status of Greenhouse Tomato Production in Aksu Area of Southern Xinjiang显示文摘With the implementation of the '13^(th) Five-Year Plan' to promote the border area and raise rural living standards, the Xinjiang Production and Construction Corps has paid more and more attention to the development of protected agriculture, which is needed for the comprehensive development and stability of the border groups. In this paper, the current status of greenhouse tomato production in Aksu of southern Xinjiang is analyzed and summarized, finding the following: the structure is not reasonable with poor performance; the soilless cultivation mode is gradually developed, and there are innovative forms; the fertilization structure is not reasonable and needs improvement; the technical level is low, and it needs to be upgraded. Therefore, to solve the various problems that exist at present, some efforts are needed achieve a better production model. | Chunling WANG Weitang SONG | 2019 | Agricultural Biotechnology2019，8，2： | 1 |
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