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	题名	作者	年代	出处	被引量
1	Production and Characterization of Anti-estrone Monoclonal Antibody显示文摘Objective Determination of estrone (E1) levels has a significant meaning in evaluating physiological effect and diagnosing some diseases. In order to detect free E1 in biological fluids, a monoclonal antibody specific for E1 was prepared after the complete antigen of E1 was synthesized. The purified monoclonal antibody was fully characterized for later immunoassay. Methods 3-O-carboxymethyl ether derivative of E1 was synthesized and in turn coupled to bovine serum albumin (BSA) to form complete antigen E1-BSA. A monoclonal antibody (McAb) specific for E1 was produced both in vitro and in vivo by a hybridoma anti-E1. Anti-E1 was prepared by fusion of SP2/0 murine myeloma cells with spleen cells isolated from immunized BALB/c mouse. The McAb was characterized by enzyme-linked immunosorbent assay (ELISA), SDS-PAGE and Western-blotting. The specificity of the immunoassay was investigated by determining the cross-reactions of E1 analogs when free E1 was detected by competitive indirect enzyme-linked immunosorbent assay (CI-ELISA). Results Analysis revealed that anti-E1 McAb (E1-McAb) was of the IgG1 type, the molecular weight of E1-McAb was 164 000 daltons. The affinity constant of E1-McAb with coated complete antigen was 8.2108L/mol. The linear range for free E1 determined by CI-ELISA was 10pg/mL^10ng/mL. The detection limit was 21.4 pg/mL (defined as twice the standard deviation of the blank). Conclusion The CI-ELISA developed with E1-McAb was both sensitive and specific. The prepared E1-McAb can be used in some immunoassays.	WangYC GuoZQ 等	2002	Biomedical and Environmental Sciences2002，15，2：	6
2	Acomputedtomo-graphicimagestudyonthethicknessoftheinfrazygomaticcrestofthemaxillaanditsclinicalimplicationsforminis-crewinsertion显示文摘	LiouEJ ChenPH WangYC etal	2007	AmJOrthodDentofacialOrthop2007，131，3：	1
3	Preva- lence, isolation and partial sequence analysis of hepa- titis E virus from domestic animals in China显示文摘	WANGYC ZHANG H Y XIANS etal	2002	J Med Virol2002，67，4：	1
4	Effectsofpropranololontheleftventricular volume of normal subjects during CT coronaryangiography显示文摘	MoYH JawFS WangYC etal	2011	KoreanJRadiol2011，12，3：	1
5	Genetic diversity and differentiation of Sclerotinia sclerotiorum populations in sunflower 显示文摘	LiZ Q WangYC ChenY ZhangJ X FernandoW G D	2009	Phytoparasitica2009，37，：	1
6	Hyperbaric oxygen preconditioning reduces ischemia-reperfusion injury by stimulating autophagy in neuro- cyte 显示文摘	WangYC Zhang S Du TY	2010	Brain Res2010，1323，4：	1
7	Treatment of periarthfitis humeroscapularis with Hyaluronidase intra-articular injection显示文摘	WangXP WangYC	2004	Clin J Med Office(Chinese)2004，32，2：	1
8	FormsofphosphorusinsedimentofLakeBaihuaandLakeHongfengGuizhou显示文摘	WangYC(王雨春) WanGJ(万国江) WangS-L(王仕禄) etal		矿物学报0，，：	1
9	Growth and sur- vival of bifidobacteria and lactic acid bacteria during the fermentation and storage of cultured soymilk drinks 显示文摘	WangYC YouR C YangH Y	2002	Food Microbiology2002，19，5：	1
10	Tissue expressions of nine genes important to immune defence of the Pacific white shrimp Litopenaeus vannamei 显示文摘	WangYC Chang P S Chen H Y etal	2007	Fish & Shellfish Immunology2007，23，6：	1
11	Detection of RASSF1A promoter hypermethylation in serum from gastric and colorectal adenoearcinoma patients显示文摘	WANGYC YUZH LIUC	2013	{H}World Journal of Gastroenterology2013，，19：	1
12	ImprovementsynthesisofthesexpheromoneofAsiancorngorer(亚洲玉米螟性信息素的改良合成)[J]显示文摘	WANGYC(汪有初) LIUFC(刘复初)		昆明师专学报自然科学版0，，：	1
13	Impact of location of epicardial adipose tissue, measured by coronary artery calcium-scoring computed tomography on obstructive coronary artery disease显示文摘	WUFZ HUANGYL WANGYC	2013	Am J Car- diol2013，112，：	1
14	ProgressesofProduceSecondaryMetabolismUsingPlantTissueCulture[J]显示文摘	LiuCC WangYC OuyangP	1997	生物技术通报1997，，5：	1
15	1 FDA drug approval sum- ma ry : pemetrexed for injection (Alimta) for the treatment of non smallcell lung cancer 显示文摘	CohenMH Johnson JR WangYC eta	2005	Oncologist2005，10，6：	1
16	Lodox/Statscan provides benefits in evaluation of gunshot injuries显示文摘	FuCY WangYC HsiehCH etal	2011	Am J Emerg Med2011，29，7：	1
17	Mutations of Cx26 gene (GJB2) for prelingual deafnessin Taiwan显示文摘	WangYC Kung CY Su MC	2002	Eur J Hum Genet2002，10，：	1
18	Treatment of periarthr'itis humeroscapularis with Hyaluronidase intra-articular injeetion显示文摘	WangXP WangYC	2004	Clin J Med Office(Chinese)2004，32，2：	1
19	Rapidspeciationanalysisofmercuryinseawater and marine fish by cation exchangechromatography hyphenated with inductively coupledplasmamassspectrometry显示文摘	ChenXP HanC ChengHY WangYC LiuJH XuZG HuL	2013	JournalofChromatographyA2013，1314，：	1
20	Effectsofnitrogenlatetopdressingstagesonproductivityofpost-anthesisphotosynthatesandgrainyieldofYangmai20显示文摘	DingJF(丁锦峰) ChenFF(陈芳芳) WangYc(王云翠) etal	2012	扬州大学学报农学与生命科学版2012，33，3：	1

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