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| 1 | The modulation of radiation-induced cell death by genistein in K562 cells:Activation of thymidine kinase 1显示文摘Ionizing radiation is one of the most effective tools in cancer therapy. In a previous study, we reported that protein tyrosine kinase (PTK) inhibitors modulate the radiation responses in the human chronic myelogenous leukemia (CML)cell line K562. The receptor tyrosine kinase inhibitor, genistein, delayed radiation-induced cell death, while non-recepter tyrosine kinase inhibitor, herbimycin A (HMA) enhances radiation-induced apoptosis. In this study, we focused on the modulation of radiation-induced cell death by genistein and performed PCR-select suppression subtractive hybridization(SSH) to understand its molecular mechanism. We identified human thymidine kinase 1 (TK1), which is cell cycle regulatory gene and confirmed expression of TK1 mRNA by Northern blot analysis. Expression of TK1 mRNA and TK 1enzymatic activity were parallel in their increase and decrease. TK1 is involved in G1-S phase transition of cell cycle progression. In cell cycle analysis, we showed that radiation induced G2 arrest in K562 cells but it was not able to sustain. However, the addition of genistein to irradiated cells sustained a prolonged G2 arrest up to 120 h. In addition,the expression of cell cycle-related proteins, cyclin A and cyclin B 1, provided the evidences of G1/S progression and G2-arrest, and their relationship with TK1 in cells treated with radiation and genistein. These results suggest that the activation of TK1 may be critical to modulate the radiation-induced cell death and cell cycle progression in irradiated K562 cells. | Min Ho JEONG Young Hee JIN Eun Young KANG Wol Soon JO Hwan Tae PARK Jae Dong LEE Yeo Jin YOO Soo Jin JEONG | 2004 | Cell Research2004，14，4： | 10 |
| 2 | Skin island flaps supplied by the vascular axis of the sensitive superficial nerves: anatomic study and clinical experience in the leg显示文摘 | Masquelet AC Romana MC Wol fG | 1992 | Plast Reconstr Surg1992，89，： | 1 |
| 3 | Vascular dementia: Diagnostic criteria for research studies: Report of the NINDS-AIREN International Workshop*显示文摘 | G. C. Román T. K. Tatemichi T. Erkinjuntti J. L. Cummings J. C. Masdeu J. H. Garcia L. Amaducci J.-M. Orgogozo A. Brun A. Hofman D. M. Moody M. D. O?Brien T. Yamaguchi J. Grafman B. P. Drayer D. A. Bennett M. Fisher J. Ogata E. Kokmen F. Bermejo P. A. Wol | 1993 | Neurology1993，，2： | 1 |
| 4 | The antigenic index,a novel algorithm for predicting antigenic determinants显示文摘 | JAMESON B A WOL F H | 1988 | Biotechnol Appl Biochem1988，4，1： | 1 |
| 5 | Anti-corrosion Properties of Zinc Dust Pants显示文摘 | Ross T K Wol Stenholme J | 1977 | Corrosion Science1977，17，： | 1 |
| 6 | Intracoronary bone marrow celltransfer after myocardial infarction:eighteen months' fol ow-up data from the randomized,control ed BOOST(BOne marrOw transfer to enhance ST-elevation infarct regeneration)trial显示文摘 | Meyer GP Wol ert KC Lotz J | | 0，，10： | 1 |
| 7 | Intracoronary bone marrow celltransfer after myocardial infarction:5-year fol ow-up from the randomized-control ed BOOST trial显示文摘 | Meyer GP Wol ert KC Lotz J | | 0，，24： | 1 |
| 8 | Optimization of culture media for solidstate culture of Pleurotus ferulae显示文摘 | WOL SUK C DUBOK C SI HYUNGK | 2004 | Biotechnology and Bioprocess Engineering2004，9，： | 1 |
| 9 | Corrosion behaviour of the amorphous Mg65Y10Cu15Ag10 alloy显示文摘 | Subba Rao R V Wol U Baunack S | 2003 | Corrosion Science2003，45，： | 1 |
| 10 | Skin island flaps sup- plied by the vascular axis of the sensitive superficial nerve: anatomic study and clinical experience in the leg 显示文摘 | Masquelet A C Romana M C Wol fg | 1992 | Plast Reconstr Surg1992，89，6： | 1 |
| 11 | Tumor-derived exosomes are a source of shared tumor rejection antigens for CTL crosspriming显示文摘 | Wol fers J Lozier A Raposo G | 2001 | Nat Med2001，7，3： | 1 |
| 12 | Ozone- in- duced oxidat ive burnt in the ozone biem onitor plant to- bacco BelWs显示文摘 | Martina S Wol - ang M Cosima W | | 0，，16： | 1 |
| 13 | Residence time distributions in ozone contactors显示文摘 | Wols BA Uijttewaal W S Riteveld L C | | 0，，01： | 1 |
| 14 | Organizational Socialization:Its Content and Consequences显示文摘 | ChaoG T OLeary-kelly A M Wol? S Klein H J Gardner P D | 1994 | Journal of Applied Psychology1994，79，5： | 1 |
| 15 | Severe Ebola virus disease with vascular leakage and multiorgan failure: treatment of a patient in intensive care显示文摘 | Wol{ T Kann G Becker S Stephan C Brodt H R de Leuw P | 2015 | Lancet2015，385，： | 1 |
| 16 | Elf eel of varying nitrogen and concentration during growing setsort on pepper flowering and fruit yield显示文摘 | H Wol S Kafkafi U | 2001 | Journal of Nutrition2001，21，10： | 1 |
| 17 | Tissue-specific pattern of stress kinase activation in ischemic/reperfused heart and kidney显示文摘 | Yin T Sandhu G Wol fgang C D | 1997 | J Biol Chem1997，272，19： | 1 |
| 18 | Carbonate debris sheets and turbidites, exuma sound, bahamas 显示文摘 | PAUL D C WOL FGAN G S | 1980 | J Sedim Petrol1980，50，11： | 1 |
| 19 | Intracoronary bone marrow celltransfer after myocardial infarction:eighteen months' fol ow-up data from the randomized,control ed BOOST(BOne marrOw transfer to enhance ST-elevation infarct regeneration)trial显示文摘 | Meyer GP Wol ert KC Lotz J | | 0，，10： | 1 |
| 20 | Evaluation of experimental techniques to validate numerical computations of the hydraulics inside a UV bench - scale reactor 显示文摘 | WOLS B A SHAO L | 2010 | Chemical Engineering Science2010，65，15： | 1 |
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