| 6 | RP-HPLC method for the quantitation of β-Sitosterol in Elaeagnus Gonyanthes Benth显示文摘Objective To establish an RP-HPLC method for the determination of β-Sitosterol in Elaeagnus Gonyanthes Benth.Methods The separation was performed on a luna C8(2)(150 mm×4.6 mm,5μm)column with the mobile phase of methanol-water(88∶12,v/v)at a flow rate of 1.0 mL/min,the detection wavelength was set at 210 nm,and the temperature of the column was maintained at 35 ℃.Results The calibration curve of β-Sitosterol was linear over the concentration range of 0.075-0.375 mg/mL(r=0.9999)and the average recovery of β-Sitosterol was 96.30% with RSD of 3.60%(n=3).Conclusion The method is simple,rapid,and accurate,and can be used for the quality control of Elaeagnus Gonyanthes Benth. | De-Xi Jiang,Wen-Zhi Chen,Dan Zhang West China School of Pharmacy,Si Chuan University,Chengdu 610041,China | 2010 | Journal of Pharmaceutical Analysis2010，22，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员