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| 1 | 黄瓜黑色果刺基因染色体定位及候选基因分析显示文摘【目的】黄瓜作为重要的果菜类蔬菜,果实品质一直是黄瓜育种研究的重点。果实品质包括内在品质和外观品质,其中外观品质对黄瓜的商品性具有重要影响。果刺颜色作为黄瓜重要的品质性状之一,对其进行遗传分析和基因定位将有助于了解果刺颜色遗传的分子机理,为黄瓜果实性状改良提供理论依据和技术支撑,同时也可为刺色基因的精细定位及克隆奠定基础。【方法】研究利用黄瓜白色果刺自交系GY14(P1)和黑色果刺自交系NC76(P2)为亲本构建遗传群体,进行黄瓜果刺颜色的遗传分析。以F2分离群体为试材,应用分离群体分组分析法(BSA)和2 112对SSR引物进行SSR分析,结合9 930黄瓜全基因组序列信息和100份核心种质重测序结果开发新标记,对初定位区域进行标记加密。采用JoinMap 4.0作图软件和MapInspect软件构建连锁群并完成连锁群与染色体的对应,实现黄瓜黑色果刺性状的基因定位。运用生物信息学,在定位区域进行候选基因分析。利用包含156个株系的重组自交系(RILs)群体,对黑色果刺基因紧密连锁的两侧翼分子标记进行验证,确定标记用于分子标记辅助选择(MAS)育种的准确性。【结果】研究表明,黄瓜自交系NC76的黑色果刺符合质量性状遗传特点,由显性单基因B控制,黑色对白色为显性。初步定位筛选获得了与B基因连锁的8对SSR引物,将B定位于黄瓜4号染色体(Chr.4)上,最近的连锁标记为SSR22231,遗传距离为10.8 cM;根据初定位区域的序列信息,设计合成了新的SSR引物212对和Indel引物25对,利用这些引物对双亲和F2群体DNA进行分析,最终构建了一个包含14个SSR标记和1个InDel标记的分子标记连锁群,获得与B连锁的两侧翼标记SSRB-181和SSRB-130,遗传距离分别为2.0 cM和1.6 cM,该区段物理距离为422.1 kb,有60个预测候选基因,推测Csa4G003095和Csa4G001690是与黑色果刺形成相关性较大的候选基因。与B紧密连锁的两侧翼标记用于MAS育种的准确率分别为96.8%和96.2%,其中SSRB-181对黑色果刺植株鉴定的准确率达到100%,将在MAS育种发挥重要作用。【结论】黄瓜自交系NC76的黑色果刺性状由显性单基因控制,该基因位于Chr.4上422.1 kb范围内,两侧翼标记为SSRB-181和SSRB-130,遗传距离为3.6 cM。本研究结果为黑色果刺基因的精细定位和克隆及MAS育种奠定了良好基础。 | 刘书林 顾兴芳 苗晗 王烨 Yiqun Weng Todd C Wehner 张圣平 | 2014 | 中国农业科学2014，47，1： | 11 |
| 2 | Adult metaplastic hutch diverticulum with robotic-assisted diverticulectomy and reconstruction:A case report显示文摘BACKGROUND Hutch diverticulum arises from the compromised muscular development at the ureteral orifice.It is a congenital disease and extremely rare in adult,only accounting for about 3%occurrence worldwide.It can be either symptomatic or asymptomatic,and relies on image tools for diagnosis and preoperative planning.Indications for surgery are dependent on the complications from the diverticulum.Metaplasia is about 10%among those with hutch diverticulum,and it still has chances turning into malignancy,especially urothelial cell carcinoma.CASE SUMMARY A 27-year-old man was presented with frequently recurrent urinary tract infection for one year,and had suffered from intermittent right flank pain for 3 mo.No past medical histories were recorded before.No obvious abnormalities on laboratory data and urine examination were found.Under ultrasound,right hydronephrosis was seen and an anatomical abnormality was observed on intravenous pyelography.Further computed tomography urogram showed one diverticulum seated at superolateral side of right ureteral orifice.Cystoscopy was done and biopsy results showed focal metaplasia.After discussing with him,roboticassisted diverticulectomy with reconstruction was performed.Right hydronephrosis was greatly improved after surgery.He has completed his 1.5-year follow-ups,and no malignancies were seen from urine cytology and image of intravenous pyelography.CONCLUSION Robotic-assisted diverticulectomy and reconstruction to hutch diverticulum is a safe and efficient operation,providing several advantages over open and laparoscopic ones. | Che H Yang Yi S Lin Yen C Ou Wei C Weng Li H Huang Chin H Lu Chao Y Hsu Min C Tung | 2020 | World Journal of Clinical Cases2020，8，20： | 2 |
| 3 | A note on Platt's probabilistic outputs for support vector machines显示文摘 | Lin H T Lin C J Weng R C | 2007 | Machine Learning2007，68，3： | 1 |
| 4 | Comparative studies on the hypolipidemic and growth suppressive effects of oolong, black, pu-erh, and green tea leaves in rats显示文摘 | Kuo K L Weng M S Chiang C T | 2005 | Journal of Agricultural and Food Chemistry2005，53，2： | 1 |
| 5 | Video object tracking using adaptive Kalman filter显示文摘 | WENG S K KUO C M TU S K | 2006 | Journal of Visual Communication and Image Representation2006，17，6： | 1 |
| 6 | Pu-erh tea supple-mentation suppresses fatty acid synthase expression in the rat liverthrough downregulating Akt and JNK signalings as demonstrated inhuman hepatoma HepG2 cells显示文摘 | CHIANG C T WENG M S LIN-SHIAU S Y | 2006 | Oncology Research Featuring Pre-clinical and Clinical Cancer Therapeutics2006，16，3： | 1 |
| 7 | Immobilizationof RGD peptide on HA coating through a chemical bondingapproach显示文摘 | YANG C L CHENG K WENG W J | 2009 | Journal of Materials Science:Materials in Medi-cine2009，20，11： | 1 |
| 8 | Effects of additives on micro-structures and microwave dielectric properties of (Zr,Sn)TiO4 ceramics显示文摘 | Huang C L Weng M H Chen H L | 2001 | Mater Chem Phys2001，71，： | 1 |
| 9 | Effects of addtives on microstructure and microwave dielectric properties of (Zr,Sn) TiO4 ceramics显示文摘 | HUANG C L WENG M H CHEN H L | 2001 | Mater Chem Phys2001，71，： | 1 |
| 10 | Surgical management of symptomatic os odontoideum with posterior screw fixation performed using the magerl and harms techniques with intraoperative 3 dimensional fluoroscopy-based navigation显示文摘 | Weng C Tian W Li ZY | 2012 | Spine2012，37，21： | 1 |
| 11 | PTEN induces apoptosis and cell cycle arrest through phosphoinosistol - 3 - kinase/Akt - dependent and independent pathways 显示文摘 | Weng L Brown JL Eng C | 2001 | Hum Mol Gent2001，10，3： | 1 |
| 12 | Adsorption of a cationic dye (methylene blue) onto spent activated clay 显示文摘 | Weng C H Part Y F | 2007 | Jour- nal of Hazardous Materials2007，144，： | 1 |
| 13 | Dynamic mag- netic forces in superconducting ceramic显示文摘 | Moon F C Weng K C Chang P Z | 1989 | J Appl Phys1989，66，3： | 1 |
| 14 | Removal of Cr(Ⅲ) from day soils by electrokinetics 显示文摘 | Weng C Yuan C | 2001 | Environmental Geochemistry and Health2001，23，3： | 1 |
| 15 | Transcutaneous oxygen pressure measurement in diabetic foot ulcers: mean va- lues and cut-point for wound healing显示文摘 | Yang C Weng H Chen L | 2013 | J Wound Osto- my Continence Nurs2013，40，6： | 1 |
| 16 | Molecular dynamics anal- ysis of temperature effects on nanoindentation measurement 显示文摘 | Fang T H Weng C I Chang J G | 2003 | Materials Science and Engineering A2003，357，12： | 1 |
| 17 | Development and validation of a sensitive and robust LC - tandem MS method for the analysis of warfarin enantiomers in human plasma 显示文摘 | Weng ND Ring PR Midtlien C | 2001 | Journal of Pharmaceutical and Biomedical Analysis2001，25，： | 1 |
| 18 | An evaluation approach to engineering design in QFD processes using fuzzy goal programming models显示文摘 | CHEN L H WENG M C | 2006 | European Journal of Operational Research2006，172，1： | 1 |
| 19 | Comparative studies on the hypolipidemic and growth suppressive effects of oolong,black,pu-erh,and green tea leaves in rats显示文摘 | KUO K L WENG M S CHIANG C T | 2005 | J Agric Food Chem2005，53，2： | 1 |
| 20 | Probability estimates for multi-class classification by pairwise coupling 显示文摘 | Wu T F LIN C J WENG R C | 2004 | Journal of Machine Learning Research2004，12，5： | 1 |
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