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| 1 | Tumor cells educate mesenchymal stromal cells to release chemoprotective and immunomodulatory factors显示文摘Factors released by surrounding cells such as cancer-associated mesenchymal stromal cells(CA-MSCs)are involved in tumor progression and chemoresistance.In this study,we characterize the mechanisms by which naYve mesenchymal stromal cells(MSCs)can acquire a CA-MSCs phenotype.Ovarian tumor cells trigger the transformation of MSCs to CA-MSCs by expressing pro-tumoral genes implicated in the chemoresistance of cancer cells,resulting in the secretion of high levels of CXC chemokine receptors 1 and 2(CXCR1/2)ligands such as chemokine(C-X-C motif)ligand 1(CXCL1),CXCL2,and interleukin 8(IL-8).CXCR1/2 ligands can also inhibit the immune response against ovarian tumor cells.Indeed,through their released factors,CA-MSCs promote the differentiation of monocytes towards M2 macrophages,which favors tumor progression.When CXCR1/2 receptors are inhibited,these CA-MSC-activated macrophages lose their M2 properties and acquire an anti-tumoral phenotype.Both ex vivo and in vivo,we used a CXCR1/2 inhibitor to sensitize ovarian tumor cells to carboplatin and circumvent the pro-tumoral effects of CA-MSCs.Since high concentrations of CXCR1/2 ligands in patients*blood are associated with chemoresistance,CXCR1/2 inhibition could be a potential therapeutic strategy to revert carboplatin resistance. | Augustin Le Naour Melissa Prat Benolt Thibault Renaud Mével Léa Lemaitre Helene Leray Marie-Veronique Joubert Kimberley Coulson Muriel Golzio Lise Lefevre Eliane Mery Alejandra Martinez Gwénael Ferron Jean-Pierre Delord Agnès Coste Bettina Couderc | 2020 | Journal of Molecular Cell Biology2020，12，3： | 3 |
| 2 | Cyto-kine and acute phase protein gene expression in repeat-ed liver biopsies of dairy cows with a lipopolysaccha-ride-induced mastitis 显示文摘 | VELS L R0NTVED C M BJERRING M | 2009 | Journal of Dairy Science2009，92，3： | 1 |
| 3 | Evaluation of seven housekeeping genes for multilocus sequence analysis of the genus Mesorhizobium : resolving the taxonomic affiliation of the Cicer canariense rhizobia显示文摘 | Juan Pérez-Yépez Natalia Armas-Capote Encarna Velázquez Ricardo Pérez-Galdona Raúl Rivas Milagros León-Barrios | 2014 | Systematic and Applied Microbiology2014，，： | 1 |
| 4 | Cubic Boron Nitride: Synthesis, Physiochemical Properties and Applications显示文摘 | Vel L Demazeav G Etourneau J | 1991 | Mater Sci Eng B1991，10，： | 1 |
| 5 | Sexual dimorphic expression pattern of asplice variant of zebrafish vasa during gonadal development显示文摘 | KRVEL A V OLSEN L C | 2004 | Develop-mental Biology2004，271，1： | 1 |
| 6 | The effect of electrical stimulation of the subthalamic nucleus on seizures is frequency dependent显示文摘 | Lado FA Velísek L Moshé SL | 2003 | Epilepsia2003，44，2： | 1 |
| 7 | Kinetic analysis of carbon monoxide and methanol oxidation on high performance carbon-supported Pt-Ru electrocatalyst for direct methanol fuel cells显示文摘 | VEL ZQUEZ-PALENZUELA A CENTELLAS F GARRIDO J A ARIAS C RODR GUEZ R M BRILLAS E CABOT P L | 2011 | J Power Sources2011，196，7： | 1 |
| 8 | A theoretical basis for the use of principal components models for monitoring multivariate processes 显示文摘 | Wise B M Ricker N L Vel Tkamp D J | 1990 | Process Control and Quality1990，1，1： | 1 |
| 9 | Fe-Nx/C electrocatalysts synthesized by pyrolysis of Fe(II)-2,3,5,6-tetra(2-pyridyl)pyrazine complex for PEM fuel cell oxygen reduction reaction显示文摘 | VELZQUEZ-PALENZUELA A ZHANG L WANG L CABOT P L BRILLAS E TSAYA K ZHANG J | 2011 | Electrochim Acta2011，56，13： | 1 |
| 10 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 11 | Electrical stimulation of substantia nigra pars reticulata is anticonvulsant in adult and young male rats显示文摘 | Velísek L Velísková J Moshé SL | 2002 | Exp Neurol2002，173，1： | 1 |
| 12 | Comparison of different ibuprofen-amino acid compounds with respect to emulsifying and cytotoxic properties显示文摘 | Baydoun L Düvel A Daniels R | 2004 | Int J Pharm2004，274，12： | 1 |
| 13 | Rapid identification of Agrobacterium species by staircase electrophoresis of low molecular weight RNA profiles显示文摘 | VEL(A)Z1QUEZ E PALOMO J L LASTRA B | 2001 | Eur J Plant Pathol2001，107，： | 1 |
| 14 | Cubic boron nitride:synthesis,physiochemical properties and applications显示文摘 | Vel L Demazeav G Etourneaum J | 1991 | Mater Sci Engng B1991，10，： | 1 |
| 15 | Kinetic of benzotriazole oxidation by ozone and hydroxyl radical 显示文摘 | VEL L N K ROSHANIB | 2010 | Water Res2010，44，6： | 1 |
| 16 | Vacuum pulse and brine composition effect on pickling kinetics of whole jalape?o pepper显示文摘 | Mujica Paz H Argüelles Pi?a L D Pérez Velázquez L C | 2006 | Innovative Food Science & Emerging Technologies2006，7，3： | 1 |
| 17 | Susceptibility of immature and adult brains to seizure effects显示文摘 | Haut S R Velísková J Moshé S L | 2004 | Lancet Neurol2004，3，10： | 1 |
| 18 | Neuroprotective effects of estrogens on hippocampal cells in adult female rats after status epilepticus显示文摘 | Velískov J Velísek L Galanopoulou AS Sperber EF | 2000 | Epilepsia2000，41，6： | 1 |
| 19 | Antifungal activity of orange ( Citrus sinensis var. Valencia) peel essential oil applied by direct addition or vapor contact显示文摘 | Maria José Velázquez-Nu?ez Raúl Avila-Sosa Enrique Palou Aurelio López-Malo | 2013 | Food Control2013，，1： | 1 |
| 20 | Multi-objective optimization of fiber reinforced composite laminates for strength, stiness and minimal mass显示文摘 | PELLETIER J L VEL S S | 2006 | Computers and Structures2006，84，12： | 1 |
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