|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 离体心脏Langendorff灌流模型稳定性的探讨显示文摘Langendorff离体心脏灌注模型凭借其较高的可重复性、良好的稳定性和相对较低的技术要求等优点,成为了心血管研究领域中最为广泛使用的实验模型之一。在获取离体心脏后,将其连接于灌注装置并通过逆行灌注的方法对离体心脏进行灌注;进行灌注时,对灌注液成分进行调整并保持灌注压力、温度和pH值的稳定,就能使离体心脏功能恢复到生理状态。所以,模型是否稳定会对实验结果产生直接的影响。近十几年来,正是由于人们充分掌握了如何建立稳定的离体心脏灌注模型,并利用该模型所具有的优势,在心血管和药理学领域的研究才不断取得重大突破。 | 梁伟涛 Rusinkevich Vitali 臧旺福 | 2011 | 国际心血管病杂志2011，38，1： | 17 |
| 2 | Gold nanorods with a hematoporphyrin-loaded silica shell for dual-modality photodynamic and photothermal treatment of tumors in vivo显示文摘由一个金 nanorod 核心和与 hematoporphyrin (HP ) 做的 mesoporous 硅石壳组成的 Nanocomposites (NC ) 被制作了以便由在 vivo 联合 photothermal 和光力学的治疗(太平洋夏季时间 + PTT ) 改进癌症治疗的效率。除了 810830 nm 附近的长波长的电浆子回声,制作 NC 展出了相应的一座 400-nm 吸收度山峰围住 HP,在 632.5-nm Q 乐队附近在 633-nm 刺激下面产生了汗衫氧,并且生产了在 808-nm 下面的热在红外线附近(NIR ) 激光照耀。这些形式被用于联合太平洋夏季时间 + PTT 治疗大(大约 3 cm3 ) 在有一只 xenorafted 肿瘤老鼠的 vivo 的稳固的肿瘤当模特儿。NC 直接被注入肿瘤并且与 633-nm 和 808-nm 激光同时照耀刺激联合光力学并且 NC 的 photothermal 活动。联合治疗的效率被光连贯断层摄影术评估,组织学的分析,并且由肿瘤体积生长的大小在一个 21 天的时期期间。调停 NC 的太平洋夏季时间在组织组织学并且到导致了弱变化一中等在肿瘤体积的 20% 减少。相反,联合太平洋夏季时间 + PTT 处理导致了大区域的肿瘤坏死并且在肿瘤体积导致了戏剧的减少。 | Georgy Terentyuk Elizaveta Panfilova Vitaly Khanadeev Daniil Chumakov Elina Genina Alexey Bashkatov Valery Tuchin Alia Bucharskaya Galina Maslyakova Nikolai Khlebtsov Boris Khlebtsov | 2014 | Nano Research2014，7，3： | 17 |
| 3 | Hepatocellular carcinoma:Surgical perspectives beyond the barcelona clinic liver cancer recommendations显示文摘The barcelona clinic liver cancer(BCLC)staging system has been approved as guidance for hepatocellular carcinoma(HCC)treatment guidelines by the main Western clinical liver associations.According to the BCLC classification,only patients with a small single HCC nodule without signs of portal hypertension or hyperbilirubinemia should undergo liver resection.In contrast,patients with intermediate-advanced HCC should be scheduled for palliative therapies,even if the lesion is resectable.Recent studies report good short-term and long-term outcomes in patients with intermediate-advanced HCC treated by liver resection.Therefore,this classification has been criticised because it excludes many patients who could benefit from curative resection.The aim of this review was to evaluate the role of surgery beyond the BCLC recommendations.Safe liver resection can be performed in patients with portal hypertension and well-compensated liver function with a 5-year survival rate of 50%.Surgery also offers good long-term result in selected patients with multiple or large HCCs with a reported 5-year survival rate of over 50%and 40%,respectively.Although macrovascular invasion is associated with a poor prognosis,liver resection provides better long-term results than palliative therapies or best supportive care.Recently,researchers have identified several genes whose altered expression influences the prognosis of patients with HCC.These genes may be useful for classifying the biological behaviour of different tumours.A revision of the BCLC classification should be introduced to provide the best treatment strategy and to ensure the best prognosis in patients with HCC. | Alfredo Guglielmi rea Ruzzenente Simone Conci Alessro Valdegamberi Marco Vitali Francesca Bertuzzo Michela De Angelis Guido Mantovani Calogero Iacono | 2014 | World Journal of Gastroenterology2014，20，24： | 16 |
| 4 | When water meets iron at Earth's core-mantle boundary显示文摘Hydrous minerals in subducted crust can transport large amounts of water into Earth's deep mantle. Our laboratory experiments revealed the surprising pressure-induced chemistry that, when water meets iron at the core-mantle boundary, they react to form an interlayer with an extremely oxygen-rich form of iron, iron dioxide, together with iron hydride. Hydrogen in the layer will escape upon further heating and rise to the crust, sustaining the water cycle. With water supplied by the subducting slabs meeting the nearly inexhaustible iron source in the core, an oxygen-rich layer would cumulate and thicken, leading to major global consequences in our planet. The seismic signature of the D″ layer may echo the chemical complexity of this layer. Over the course of geological time, the enormous oxygen reservoir accumulating between the mantle and core may have eventually reached a critical eruption point. Very large-scale oxygen eruptions could possibly cause major activities in the mantle convection and leave evidence such as the rifting of supercontinents and the Great Oxidation Event. | Ho-Kwang Mao Qingyang Hu Liuxiang Yang Jin Liu Duck Young Kim Yue Meng Li Zhang Vitali B.Prakapenka Wenge Yang Wendy L.Mao | 2017 | National Science Review2017，4，6： | 14 |
| 5 | Using the polymerase chain reaction coupled with denaturing gradient gel electrophoresis to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis显示文摘AIM: To investigate the use of PCR and DGGE to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted severe AP.METHODS: Patients with biochemical and clinical evidence of acute pancreatitis and an APACHE Ⅱ score ≥8 were enrolled. PCR and DGGE were employed to detect bacterial translocation in blood samples collected on d1,3, and 8 after the admission. Standard microbial blood cultures were taken when there was clinical evidence of sepsis or when felt to be clinically indicated by the supervising team.RESULTS: Six patients were included. Of all the patients investigated, only one developed septic complications;the others had uneventful illness. Bacteria were detected using PCR in 4 of the 17 collected blood samples. The patient with sepsis was PCR-positive in two samples (taken on d 1 and 3), despite three negative blood cultures. Using DGGE and specific primers, the bacteria in all blood specimens which tested positive for the presence of bacterial DNA were identified as E coli.CONCLUSION: Our study confirmed thatunlike traditional microbiological techniques, PCR can detect the presence of bacteria in the blood of patients with severe AP. Therefore, this latter method in conjunction with DGGE is potentially an extremely useful tool in predicting septic morbidity and evaluating patients with the disease. Further research using increased numbers of patients, in particular those patients with necrosis and sepsis, is required to assess the reliability of PCR and DGGE in the rapid diagnosis of infection in AP. | Callum B Pearce Vitaly Zinkevich Iwona Beech Viera Funjika Ana Garcia Ruiz Afraa Aladawi Hamish D Duncan | 2005 | World Journal of Gastroenterology2005，11，45： | 11 |
| 6 | Presepsin teardown- pitfalls of biomarkers in the diagnosis and prognosis of bacterial infection in cirrhosis显示文摘AIM To evaluate the diagnostic and prognostic value of presepsin in cirrhosis-associated bacterial infections. METHODS Two hundred and sixteen patients with cirrhosis were enrolled. At admission, the presence of bacterial infections and level of plasma presepsin, serum C-reactive protein(CRP) and procalcitonin(PCT) were evaluated. Patients were followed for three months to assess the possible association between presepsin level and short-term mortality.RESULTS Present 34.7 of patients had bacterial infection. Presepsin levels were significantly higher in patients with infection than without(median, 1002 pg/m L vs 477 pg/m L, P < 0.001), increasing with the severity of infection [organ failure(OF): Yes vs No, 2358 pg/m L vs 710 pg/m L, P < 0.001]. Diagnostic accuracy of presepsin for severe infections was similar to PCT and superior to CRP(AUC-ROC: 0.85, 0.85 and 0.66, respectively, P = NS for presepsin vs PCT and P < 0.01 for presepsin vs CRP). At the optimal cut-off value of presepsin > 1206 pg/m L sensitivity, specificity, positive predictive values and negative predictive values were as follows: 87.5%, 74.5%, 61.8% and 92.7%. The accuracy of presepsin, however, decreased in advanced stage of the disease or in the presence of renal failure, most probably because of the significantly elevated presepsin levels in non-infected patients. 28-d mortality rate was higher among patients with > 1277 pg/m L compared to those with ≤ 1277 pg/m L(46.9% vs 11.6%, P < 0.001). In a binary logistic regression analysis, however, only PCT(OR = 1.81, 95%CI: 1.09-3.01, P = 0.022) but neither presepsin nor CRP were independent risk factor for 28-d mortality after adjusting with MELD score and leukocyte count.CONCLUSION Presepsin is a valuable new biomarker for defining severe infections in cirrhosis, proving same efficacy as PCT. However, it is not a useful marker of short-term mortality. | Maria Papp Tamas Tornai Zsuzsanna Vitalis Istvan Tornai David Tornai Tamas Dinya Andrea Sumegi Peter Antal-Szalmas | 2016 | World Journal of Gastroenterology2016，22，41： | 9 |
| 7 | Temporal dynamics of immune response following prolonged myocardial ischemia/reperfusion with and without cyclosporine A显示文摘Understanding the dynamics of the immune response following late myocardial reperfusion is critical for the development of immunomodulatory therapy for myocardial infarction (MI). Cyclosporine A (CSA) possesses multiple therapeutic applications for MI, but its effects on the inflammation caused by acute MI are not clear. This study aimed to determine the dynamics of the immune response following myocardial ischemia/reperfusion (I/R) and the effects of CSA in a mouse model of prolonged myocardial ischemia designated to represent the human condition of late reperfusion. Adult C57BL/6 mice were subjected to 90 min of closed-chest myocardial I/R, which induced severe myocardial injury and excessive infiammation in the heart. Multicomponent analysis of the immune response caused by prolonged I/R revealed that the peak of cytokines/chemokines in the systemic circulation was synchronized with the maximal infiux of neutrophils and T-cells in the heart 1 day after MI. The peak of cytokine/chemokine secretion in the infarcted heart coincided with the maximal macrophage and natural killer cell infiltration on day 3 after MI. The cellular composition of the mediastinal lymph nodes changed similarly to that of the infarcted hearts. CSA (10 mg/kg/day) given after prolonged I/R impaired heart function, enlarged the resulting scar, and reduced heart vascularization. It did not change the content of immune cells in hearts exposed to prolonged I/R, but the levels of MCP-1 and MIP-1α(hearts) and IL-12 (hearts and serum) were signi?cantly reduced in the CSA-treated group in comparison to the untreated group, indicating alterations in immune cell function. Our findings provide new knowledge necessary for the development of immunomodulatory therapy targeting the immune response after prolonged myocardial ischemia/reperfusion. | Vitali Rusinkevich Yin Huang Zhong-yan Chen Wu Qiang Yi-gang Wang Yu-fang Shi Huang-tian Yang | 2019 | Acta Pharmacologica Sinica2019，40，9： | 5 |
| 8 | Gut barrier failure biomarkers are associated with poor disease outcome in patients with primary sclerosing cholangitis显示文摘AIM To assess the prevalence of a panel of serologic markers that reflect gut barrier dysfunction in a mixed cohort of pediatric and adult primary sclerosing cholangitis(PSC) patients.METHODS Sera of 67 PSC patients [median age(range): 32(5-79) years, concomitant IBD: 67% and cirrhosis: 20%] were assayed for the presence of antibodies against to F-actin(AAA Ig A/Ig G) and gliadin(AGA Ig A/Ig G)] and for serum level of intestinal fatty acid-binding protein(I-FABP) by ELISA. Markers of lipopolysaccharide(LPS) exposure [LPS binding protein(LBP)] and various antimicrobial antibodies [anti-OMP Plus Ig A and endotoxin core Ig A antibody(Endo CAb)] were also determined. Poor disease outcome was defined as orthotopic liver transplantation and/or liver-related death during the follow-up [median: 99(14-106) mo]. One hundred and fifty-three healthy subjects(HCONT) and 172 ulcerative colitis(UC) patients were the controls. RESULTS A total of 28.4%, 28.0%, 9% and 20.9% of PSC patients were positive for AAA Ig A, AAA Ig G, AGA Ig A and AGA Ig G, respectively. Frequencies of AAA Ig A and AAA Ig G(P < 0.001, for both) and AGA Ig G(P = 0.01, for both) but not AGA Ig A were significantly higher compared to both of the HCONT and the UC groups. In survival analysis, AAA Ig A-positivity was revealed as an independent predictor of poor disease outcome after adjusting either for the presence of cirrhosis [HR = 5.15(1.27-20.86), P = 0.022 or for the Mayo risk score(HR = 4.24(0.99-18.21), P = 0.052]. AAA Ig A-positivity was significantly associated with higher frequency of antimicrobial antibodies(P < 0.001 for Endo Cab Ig A and P = 0.012 for anti-OMP Plus Ig A) and higher level of the enterocyte damage marker(median I-FABP_(AAA Ig A pos vs neg): 365 vs 166 pg/m L, P = 0.011), but not with serum LBP level. CONCLUSION Presence of Ig A type AAA identified PSC patients with progressive disease. Moreover, it is associated with enhanced mucosal immune response to various microbial antigens and enterocyte damage further highlighting the importance of the gut-liver interaction in PSC. | Tamas Tornai Eszter Palyu Zsuzsanna Vitalis Istvan Tornai David Tornai Peter Antal-Szalmas Gary L Norman Zakera Shums Gabor Veres Antal Dezsofi Gabriella Par Alajos Par Peter Orosz Ferenc Szalay Peter Laszlo Lakatos Maria Papp | 2017 | World Journal of Gastroenterology2017，23，29： | 5 |
| 9 | Surface-enhanced Raman scattering inside Au@Ag core/shell nanorods显示文摘有在他们内嵌入的 Raman 活跃的分子的 plasmonic nanoparticles 的设计和合成具有为察觉到和成像应用程序的重要兴趣。然而,有可控制的形状,尺寸,和 plasmonic 性质的如此的 nanostructures 的直接合成仍然保持极其挑战性。这里,我们在厚度与 1 ~ 25 nm 的可控制的 Ag 壳在一致 Au@Ag 核心 / 壳 nanorods 的准备报导。1,4-Aminothiophenol (4-ATP ) 分子,用作拉曼记者,在 Au 核心和 Ag 壳之间被定位。在核心 / 壳 nanoparticles 内的记者分子的成功的嵌入被氨基的组的选择氧化的缺席证实,由拉曼光谱学测量了。在里面并且在 nanorods 外面的记者分子的地点上的拉曼紧张的依赖被学习。在内部的分子给强壮、一致的拉曼看了紧张,至少比 nanoparticle 表面上的分子的高的一个数量级。与平常的 surface-functionalized 拉曼标签相对照,聚集并且与嵌入的分子 nanoparticles 聚类减少了散布的提高表面的拉曼(重量的单位) 信号。从这研究的调查结果为在核心 / 壳金属 nanostructures 内利用分子的高重量的单位反应的低 analyte 集中的一种新奇察觉技术提供基础。作为一个例子,我们显示出在答案象 10 9 M 一样低的 thiram 杀真菌剂的柔韧的重量的单位察觉。 | Boris Khlebtsov | 2016 | Nano Research2016，9，8： | 5 |
| 10 | Radiofrequency ablation vs surgical resection in elderly patients with hepatocellular carcinoma in Milan criteria显示文摘BACKGROUND Surgical resection and radiofrequency ablation(RFA)represent two possible strategy in treatment of hepatocellular carcinoma(HCC)in Milan criteria.AIM To evaluate short-and long-term outcome in elderly patients(>70 years)with HCC in Milan criteria,which underwent liver resection(LR)or RFA.METHODS The study included 594 patients with HCC in Milan criteria(429 in LR group and 165 in RFA group)managed in 10 European centers.Statistical analysis was performed using the Kaplan-Meier method before and after propensity score matching(PSM)and Cox regression.RESULTS After PSM,we compared 136 patients in the LR group with 136 patients in the RFA group.Overall survival at 1,3,and 5 years was 91%,80%,and 76%in the LR group and 97%,67%,and 41%in the RFA group respectively(P=0.001).Diseasefree survival at 1,3,and 5 years was 84%,60%and 44%for the LR group,and 63%,36%,and 25%for the RFA group(P=0.001).Postoperative Clavien-Dindo IIIIV complications were lower in the RFA group(1%vs 11%,P=0.001)in association with a shorter length of stay(2 d vs 7 d,P=0.001).In multivariate analysis,Model for End-stage Liver Disease(MELD)score(>10)[odds ratio(OR)=1.89],increased value of international normalized ratio(>1.3)(OR=1.60),treatment with radiofrequency(OR=1.46),and multiple nodules(OR=1.19)were independent predictors of a poor overall survival while a high MELD score(>10)(OR=1.51)and radiofrequency(OR=1.37)were independent factors associated with a higher recurrence rate.CONCLUSION Despite a longer length of stay and a higher rate of severe postoperative complications,surgery provided better results in long-term oncological outcomes as compared to ablation in elderly patients(>70 years)with HCC in Milan criteria. | Maria Conticchio Riccardo Inchingolo Antonella Delvecchio Letizia Laera Francesca Ratti Maximiliano Gelli Ferdinando Anelli Alexis Laurent Giulio Vitali Paolo Magistri Giacomo Assirati Emanuele Felli Taiga Wakabayashi Patrick Pessaux Tullio Piardi Fabrizio di Benedetto Nicola de'Angelis Javier Briceño AntonioRampoldi RenèAdam Daniel Cherqui Luca Antonio Aldrighetti Riccardo Memeo | 2021 | World Journal of Gastroenterology2021，27，18： | 4 |
| 11 | Molecular-Genetic Characterization of Human Rotavirus A Strains Circulating in Moscow, Russia(2009–2014)显示文摘Enteric viruses are the most common cause of acute gastroenteritis(AGE) in young children and a significant public health problem globally. Hospital admissions of children under 5 years of age with diarrhea are primarily associated with group A rotavirus(RVA) infection. In this retrospective study, the population structure of viruses linked to AGE etiology in young children hospitalized with AGE in Moscow was evaluated, and molecular characterization of RVA strains was performed.Fecal specimens were collected from children under 5 years old hospitalized with AGE between 2009 and 2014 in Moscow, Russia. Multiplex real-time reverse transcription PCR was used to detect enteric viruses and for G/[P]-genotyping of isolated RVAs. Sequencing of RVA VP7 and VP4 cDNA fragments was used to validate the data obtained by PCRgenotyping. The main causes for hospitalization of children with AGE were RVA(40.1%), followed by noroviruses(11.4%), while adenoviruses, astroviruses, sapoviruses, enteroviruses, and orthoreoviruses were detected in 4.7%, 1.9%,1.4%, 1.2%, and 0.2% of samples tested, respectively. Nosocomial infections, predominantly associated with RVAs and noroviruses, were detected in 24.8% of cases and occurred significantly more frequently in younger infants. The predominant RVA genotype was G4 P[8], detected in 38.7% of RVA-positive cases, whereas genotypes G1 P[8], G9 P[8],G3 P[8], and G2P[4] were found in 11.8%, 6.6%, 4.2%, and 3.3% of cases, respectively. Together, the presence of circulating RVA strains with rare VP7 and VP4 gene variants(G6 and P[9]) highlights the need to conduct continuous epidemiological monitoring of RVA infection. | Victoria Kiseleva Evgeny Faizuloev Elena Meskina Anna Marova Alexey Oksanich Tatiana Samartseva Georgy Bakhtoyarov Natalia Bochkareva Nikolay Filatov Andrey Linok Yulia Ammour Vitaly Zverev | 2018 | Virologica Sinica2018，33，4： | 4 |
| 12 | To Gate, or Not to Gate: Regulatory Mechanisms for Intercellular Protein Transport and Virus Movement in Plants显示文摘Cell-to-cell 信号 transduction 为安排多细胞的有机体的整个身体的生理学是重要的,并且许多内长的大分子,蛋白质,和 nucleic 酸作为如此的搬运信号工作。在植物,许多通过 plasmodesmata (Pd ) 这些分子被搬运,房间互连植物房间的跨越墙的隧道结构。而且, Pd 也为演变盗版这些隧道散布感染的大多数植物病毒的 cell-to-cell 运动充当水管。Pd 运输被假定高度选择,并且仅仅分子的一个有限全部剧目通过这些隧道被搬运。最近的研究开始解开了活跃地调整 Pd 隧道的开始到的机制允许交通。在房间之间的这 macromolecular 运输包括二连续的步:通过隧道的细胞内部的指向到 Pd 和 translocation。这里,我们考察不过被搬运的分子的种类的当前的知识控制这交通的 Pd 和机制。通常, Pd 交通能通过 trans-Pd 细胞质或通过 trans-Pd 的膜 / 腔由被动散开发生嗯,或由活跃运输,那包括 proteinprotein 相互作用。它是涉及大多数信号分子的细胞间的交通并且被不同、有时相互依赖的机制调整的 Pd 运输的这个后者模式,它代表这篇文章的焦点。 | Shoko Ueki Vitaly Citovsky | 2011 | Molecular Plant2011，4，5： | 3 |
| 13 | Effects of probiotic bacteria on gastrointestinal motility in guinea-pig isolated tissue显示文摘AIM: To evaluate the intestinal motility changes evoked by 8 bacterial strains belonging to Bifidobacterium , Lactobacillus and Streptococcus genera within the probiotic preparation VSL#3. METHODS: Ileum and proximal colon segments isolated from guinea-pigs were used as a study model. Entire cells and cell fractions (cell debris, cell wall fraction, cytoplasmatic fraction, proteinaceous and non- proteinaceous cytoplasmatic components) of VSL#3 strains and, as controls, Escherichia coli, Salmonella aboni and Bacillus licheniformis were tested in this in vitro model. RESULTS: Among the bacterial cell fractions tested, only the cytoplasmatic fraction modified intestinal motility. Lactobacillus strains stimulated the contraction of ileum segment, whereas all probiotic strains tested induced proximal colon relaxation response. The non-proteinaceous cytoplasmatic components were responsible for the colon relaxation. CONCLUSION: The results obtained in this study suggest that the proximal colon relaxation activity showed by the probiotic bacteria could be one of the possible mechanisms of action by which probiotics exert their positive effects in regulating intestinal motility. | Matteo Massi Pierfranco Ioan Roberta Budriesi Alberto Chiarini Beatrice Vitali Karen M Lammers Paolo Gionchetti Massimo Campieri Anthony Lembo Patrizia Brigidi | 2006 | World Journal of Gastroenterology2006，12，37： | 3 |
| 14 | Structure of the Ca2+-dependent PP2A heterotrimer and insights into Cdc6 dephosphorylation显示文摘B″蛋白质磷酸酶 2A (PP2A ) 的 /PR72 家庭一个重要 PP2A 家庭涉及多样的细胞的过程,并且特别地由钙绑定调整了到规章的子单元。在这个家庭的 PR70 子单元与房间部门控制 6 交往(Cdc6 ) ,为 DNA 复制的控制重要的一个房间周期管理者。这里,我们在 2.1 和 2.4 Å 的一个决定报导孤立的 PR72 和 trimeric PR70 holoenzyme 的水晶结构;,分别地并且在 Cdc6 dephosphorylation 的 vitro 描述。holoenzyme 结构表明 PR70 钙绑定主题之一直接联系支架子单元,导致在所有 PP2A holoenzymes 之中的最紧缩的支架子单元符合构造。PR70 也在活跃地点附近区别地绑在催化子单元,它被要求让 PR70 向 Cdc6 提高磷酸酶活动。我们的研究为 B″ 的唯一的规定提供一个结构的基础;由钙离子的 /PR72 holoenzymes,并且在 PP2A holoenzymes 之中为底层特性的精确控制建议机制。 | Nathan Wlodarchak Feng Guo Kenneth A Satyshur Li Jiang Philip D Jeffrey Tingwan Sun Vitali Stanevich Marc C Mumby Yongna Xing | 2013 | Cell Research2013，23，7： | 3 |
| 15 | Moving of a submarine under an ice cover in fluid of finite depth显示文摘The influence of a basin depth on ice cover deflections, when a submarine moves under it in fluid, is experimentally and theoretically analyzed. For a theoretical research the ice cover is modelled by an elastic floating plate, and the submarine is modelled by a source-sink system in a fluid flow. The problem is solved by means of asymptotic analysis and integral transformations. The experimental study is conducted with submarine models in the pool with the sizes L*B*H= 10m*3m*lm . The ice cover for experiments is modelled in two ways. The first way is the thin ice plate received by freezing of the surface layer of water at low temperatures. In the second case the rubber sheet floating on a reservoir surface is used. The special velocity (the velocity at which maximum values of the wave height are reached) is analyzed. It is obtained that the decrease of the basin depth leads to decrease of the special speed. For small speeds (less than the special speed) there is the fact that the basin depth decrease leads to the increase of the height of the flexural-gravity wave. | Alexandra V. Pogorelova Vitali L. Zemlyak Victor M. Kozin | 2019 | Journal of Hydrodynamics2019，31，3： | 3 |
| 16 | Antibiotics and probiotics in chronic pouchitis:A comparative proteomic approach显示文摘AIM:To profile protein expression in mucosal biopsies from patients with chronic refractory pouchitis following antibiotic or probiotic treatment,using a comparative proteomic approach. METHODS:Two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ ionization-time of flight mass spectrometry were used to characterize the changes related to antibiotic therapy in the protein expression profiles of biopsy samples from patients with chronic refractory pouchitis.The same proteomic approach was applied to identify differentially expressed proteins in the non-inflamed pouch before and after probiotic administration. RESULTS:In the first set of 2D gels,26 different proteins with at least 2-fold changes in their expression levels between the pouchitis condition and antibiotic-induced remission were identified.In the second set of analysis,the comparison between mucosal biopsy proteomes in the normal and probiotic-treated pouch resulted in 17 significantly differently expressed proteins.Of these,8 exhibited the same pattern of deregulation as in the pouchitis/pouch remission group. CONCLUSION:For the first time,2D protein maps of mucosal biopsies from patients with ileal pouch-anal anastomosis were provided,and differentially expressed proteins following antibiotic/probiotic treatment were identified. | Silvia Turroni Beatrice Vitali Marco Candela Paolo Gionchetti Fernando Rizzello Massimo Campieri Patrizia Brigidi | 2010 | World Journal of Gastroenterology2010，16，1： | 3 |
| 17 | Structural basis of PP2A activation by PTPA, an ATP- dependent activation chaperone显示文摘蛋白质磷酸酶 2A (PP2A ) 的合适的激活催化子单元为复杂 PP2A 规定是中央的并且为细胞的功能的宽广方面是关键的。PP2A 的水晶结构跳了到 PP2A 磷酸酶使活跃之物(PTPA ) 和 ATPγ S 表明 PTPA 与包围 PP2A 的结构的元素做宽广接触活跃地点和 ATP 的腺嘌一半。PTPA 有约束力稳定为激活要求的 apo-PP2A 的蛋白质褶层,并且东方 ATP phosphoryl 组织直接绑在 PP2A 活跃地点。这允许 ATP 调制 PP2A 活跃地点的金属绑定偏爱并且利用 PP2A 为 ATP 水解作用的活跃地点。在 vitro, ATP 有选择地并且急速地提高内长的催化金属离子的绑定,它要求 ATP 水解作用并且为 pSer/Thr-specific 磷酸酶活动的获得是关键的。而且, PP2A 有约束力、 ATP 有约束力在房间增长和幸存为 PTPA 功能被要求。我们的结果与结构的经济和能潜在地用作一个特定的治疗学的目标的一个唯一的 ATP 有约束力的衣袋在 PP2A 激活建议 PTPA 的新奇机制。 | Feng Guo Vitali Stanevich Nathan Wlodarchak Ritupama Sengupta Li Jiang Kenneth A Satyshur Yongna Xing | 2014 | Cell Research2014，24，2： | 3 |
| 18 | The Mg-carbonate-Fe interaction:Implication for the fate of subducted carbonates and formation of diamond in the lower mantle显示文摘The fate of subducted carbonates in the lower mantle and at the core-mantle boundary was modelled via experiments in the MgCO3-Fe^0 system at 70-150 GPa and 800-2600 Kin a laser-heated diamond anvil cell.Using in situ synchrotro n X-ray diffraction and ex situ transmission electron microscopy we show that the reduction of Mg-carbonate can be exemplified by:6 MgCO3+19 Fe=8 FeO+10(Mg0.6Fe^0.4)O+Fe7 C3+3 C.The presented results suggest that the interaction of carbonates with Fe^0 or Fe^0-bearing rocks can produce Fe-carbide and diamond,which can accumulate in the D'region,depending on its carbon to Fe ratio.Due to the sluggish kinetics of the transformation,diamond can remain metastable at the core-mantle boundary(CMB)unless it is in a direct contact with Fe-metal.In addition,it can be remobilized by redox melting accompanying the generation of mantle plumes. | Naira S.Martirosyan Konstantin D. Litasov Sergey S. Lobanov Alexander F. Goncharov Anton Shatskiy Hiroaki Ohfuji Vitali Prakapenka | 2019 | Geoscience Frontiers2019，10，4： | 2 |
| 19 | Calibration of the Lorentz force flowmeter显示文摘 | Vitaly Minchenya Christian Karcher Yuri Kolesnikov André Thess | 2011 | Flow Measurement and Instrumentation2011，，3： | 2 |
| 20 | 小剂量环孢素对大鼠心脏移植的免疫抑制效果显示文摘目的观察同种异基因大鼠腹部异位心脏移植术后采用不同方案给予小剂量环孢素的免疫抑制效果。方法以SD大鼠为供体,Wistar大鼠为受体建立腹部异位心脏移植模型。随机分为3组,每组6只。对照组:移植术后不进行免疫抑制;小剂量环孢素短期给药组:移植术后当天起给予2mg/(kg·d)小剂量环孢素,连续10 d;小剂量环孢素长期给药组:移植术后当天起长期给予2 mg/(kg·d)小剂量环孢素,直至供心死亡或实验终止(术后40 d)。记录各组移植心脏存活时间,并行病理学检查。结果各组供心存活时间为:对照组(6.83±0.93)d;小剂量环孢素短期给药组(16.25±1.70)d;小剂量环孢素长期给药组(38.42±2.50)d。病理结果显示对照组与小剂量环孢素短期给药组移植心脏出现大量单个核炎症细胞渗出和浸润,心肌实质损伤严重,与之相比小剂量环孢素长期给药组心肌实质损伤较轻,冠状动脉内膜出现增生性病变。结论使用同种异基因SD和Wistar大鼠建立腹部异位心脏移植模型,术后短期给予2 mg/(kg·d)小剂量环孢素能在一定程度上抑制移植心脏的急性排斥反应,长期给药能够诱导移植心脏发生慢性排斥反应。 | 李洲斌 韩晨俊 R Vitali 臧旺福 | 2010 | 同济大学学报(医学版)2010，31，4： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员